目的:系统分析髓鞘碱性蛋白(MBP)在不同程度缺血缺氧性脑病(HIE)新生儿血清水平变化。方法:计算机检索中国知网、万方数据、PubMed、Embase、Web of Science和Cochrane Library等数据库,从建库至2016-11-25收录的有关HIE新生儿MBP血清...目的:系统分析髓鞘碱性蛋白(MBP)在不同程度缺血缺氧性脑病(HIE)新生儿血清水平变化。方法:计算机检索中国知网、万方数据、PubMed、Embase、Web of Science和Cochrane Library等数据库,从建库至2016-11-25收录的有关HIE新生儿MBP血清水平变化的研究。对符合纳入和排除标准的随机或半随机对照研究进行数据提取和质量评估后,用RevMan5.3软件对文献数据进行Meta分析。结果:共检索文献156篇,最终纳入6篇。6篇文献结果数据均完整,对结局指标均有报道,不存在选择性结果报道,其它潜在偏倚风险不确定。Meta分析显示,与对照组相比,轻度、中度和重度HIE均可引起新生儿外周血MBP水平升高,且重度>中度>轻度。轻度HIE新生儿MBP水平合并标准化均数差(SMD)=1.82(95%CI:0.65-2.99,P<0.01),中度HIE新生儿MBP水平SMD=4.02(95%CI:1.55-6.49,P<0.01),重度HIE新生儿MBP水平SMD=6.06(95%CI:3.35-8.78,P<0.01)。中度与轻度HIE相比,MBP水平SMD=2.46(95%CI:0.71-4.22,P<0.01);重度与轻度HIE相比,MBP水平SMD=4.85(95%CI:2.59-7.10,P<0.01);重度与中度HIE相比,MBP水平SMD=2.94(95%CI:1.21-4.67,P<0.01)。纳入6项研究存在明显发表偏倚。结论:不同程度HIE新生儿外周血MBP水平升高可为其临床诊断提供帮助。展开更多
植物生长型和生活型是理解植物响应和适应环境变化的重要形态性状,对研究植物与环境和生态系统功能的关系,理解植物地理分布格局和生物多样性形成与维持机制具有重要意义。《中国植物志》和Flora of China中有关植物生长型和生活型的描...植物生长型和生活型是理解植物响应和适应环境变化的重要形态性状,对研究植物与环境和生态系统功能的关系,理解植物地理分布格局和生物多样性形成与维持机制具有重要意义。《中国植物志》和Flora of China中有关植物生长型和生活型的描述存在分类标准不统一,部分信息缺失、错误或模糊不清等问题。本文基于全国和地方植物志书及野外调查的植物性状信息,订正了中英文版维管植物的生长型和生活型性状,建立了中国维管植物生长型和生活型数据集。该数据集包含35,329种维管植物,内容包括植物名称、生长型和生活型性状及其数据来源,其中植物名称包括来自《中国植物志》、Flora of China和来自《中国生物物种名录(2023版)》的两个分类系统版本,以便读者相互对照不同分类系统的差异。依据茎木质化程度分为木本植物和草本植物两类,木本植物有12,600种,占35.7%;草本植物为22,729种,占64.3%。木本植物以中灌木生长型(2,917种,占23.2%)和常绿生活型(7,107种,占56.4%)为主,草本植物以杂类草生长型(19,900种,占87.6%)和多年生生活型(19,554种,占86.0%)为主。木本生长型中乔木物种以樟科和壳斗科占比较大,灌木物种以杜鹃花科、蔷薇科和豆科占比较大;常绿生活型以杜鹃花科占比较大,落叶生活型以蔷薇科占比较大。草本生长型中杂类草以菊科占比较大,禾草以禾本科占比较大;生活型中一年生、二年生和多年生分别以禾本科、紫草科和菊科占优势。本数据集涵盖了目前中国所有维管植物物种的生长型和生活型信息,可为植物性状研究提供数据基础。展开更多
BACKGROUND Situs inversus totalis(SIT)is an extremely rare congenital malformation characterized by mirror displacement of the thoracoabdominal organs such as the heart,liver,spleen,and stomach.Herein,we describe a pa...BACKGROUND Situs inversus totalis(SIT)is an extremely rare congenital malformation characterized by mirror displacement of the thoracoabdominal organs such as the heart,liver,spleen,and stomach.Herein,we describe a patient with SIT complicated with cholangiocarcinoma who underwent successful pancreaticoduodenectomy with the assistance of a da Vinci robot.CASE SUMMARY A 58-year-old female presented to the hospital with paroxysmal pain in her left upper abdomen,accompanied by jaundice and staining of the sclera as chief complaints.Imaging examination detected a mass at the distal end of the common bile duct,with inverted thoracic and abdominal organs.Endoscopic retrograde cholangiopancreatography forceps biopsy revealed the presence of a well-differentiated adenocarcinoma.The patient successfully underwent robotic-assisted pancreaticoduodenectomy;the operation lasted 300 min,the intraoperative blood loss was 500 mL,and there were no intraoperative and postoperative complications.CONCLUSION SIT is not directly related to the formation of cholangiocarcinoma.Detailed preoperative imaging examination is conducive to disease diagnosis and also convenient for determining the feasibility of tumor resection.Robot-assisted pancreaticoduodenectomy for SIT complicated with cholangiocarcinoma provides a safe,feasible,minimally invasive,and complication-free alternative with adequate preoperative planning combined with meticulous intraoperative procedures.展开更多
Background:Collagen type Ⅳ(COL4)-related nephropathy includes a variety of kidney diseases that occur with or without extra-renal manifestations caused by COL4A3-5 mutations.Previous studies revealed several novel mu...Background:Collagen type Ⅳ(COL4)-related nephropathy includes a variety of kidney diseases that occur with or without extra-renal manifestations caused by COL4A3-5 mutations.Previous studies revealed several novel mutations,including three COL4A3 missense mutations (G619R,G801R,and C1616Y) and the COL4A3 chr:228172489delA c.4317delA p.Thr1440ProfsX87 frameshift mutation that resulted in a truncated NC1 domain (hereafter named COL4A3 c.4317delA);however,the mutation mechanisms that lead to podocyte injury remain unclear.This study aimed to further explore the mutation mechanisms that lead to podocyte injury.Methods:Wild-type (WT) and four mutant COL4A3 segments were constructed into a lentiviral plasmid,then stably transfected into human podocytes.Real-time polymerase chain reaction and Western blotting were applied to detect endoplasmic reticulum stress (ERS)-and apoptosis-related mRNA and protein levels.Then,human podocytes were treated with MG132 (a proteasome inhibitor) and brefeldin A (a transport protein inhibitor).The human podocyte findings were verified by the establishment of a mus-Col4a3 knockout mouse monoclonal podocyte using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Casg) technology.Results:Our data showed that COL4A3 mRNA was significantly overexpressed in the lentivirus stably transfected podocytes.Moreover,the COL4A3 protein level was significantly increased in all groups except the COL4A3 c.4317delA group.Compared to the other test groups,the COL4A3 c.4317delA group showed excessive ERS and apoptosis.Podocytes treated with MG 132 showed remarkably increased intra-cellular expression of the COL4A3 c.4317delA mutation.MG132 intervention improved higher ERS and apoptosis levels in the COL4A3 c.4317delA group.Mouse monoclonal podocytes with COL4A3 chr:82717932insA c.4852insA p.Arg1618ThrfsX4 were successfully acquired;this NC1-truncated mutation suggested a higher level of ERS and relatively remarkable level of apoptosis compared to that of the WT展开更多
文摘目的:系统分析髓鞘碱性蛋白(MBP)在不同程度缺血缺氧性脑病(HIE)新生儿血清水平变化。方法:计算机检索中国知网、万方数据、PubMed、Embase、Web of Science和Cochrane Library等数据库,从建库至2016-11-25收录的有关HIE新生儿MBP血清水平变化的研究。对符合纳入和排除标准的随机或半随机对照研究进行数据提取和质量评估后,用RevMan5.3软件对文献数据进行Meta分析。结果:共检索文献156篇,最终纳入6篇。6篇文献结果数据均完整,对结局指标均有报道,不存在选择性结果报道,其它潜在偏倚风险不确定。Meta分析显示,与对照组相比,轻度、中度和重度HIE均可引起新生儿外周血MBP水平升高,且重度>中度>轻度。轻度HIE新生儿MBP水平合并标准化均数差(SMD)=1.82(95%CI:0.65-2.99,P<0.01),中度HIE新生儿MBP水平SMD=4.02(95%CI:1.55-6.49,P<0.01),重度HIE新生儿MBP水平SMD=6.06(95%CI:3.35-8.78,P<0.01)。中度与轻度HIE相比,MBP水平SMD=2.46(95%CI:0.71-4.22,P<0.01);重度与轻度HIE相比,MBP水平SMD=4.85(95%CI:2.59-7.10,P<0.01);重度与中度HIE相比,MBP水平SMD=2.94(95%CI:1.21-4.67,P<0.01)。纳入6项研究存在明显发表偏倚。结论:不同程度HIE新生儿外周血MBP水平升高可为其临床诊断提供帮助。
文摘植物生长型和生活型是理解植物响应和适应环境变化的重要形态性状,对研究植物与环境和生态系统功能的关系,理解植物地理分布格局和生物多样性形成与维持机制具有重要意义。《中国植物志》和Flora of China中有关植物生长型和生活型的描述存在分类标准不统一,部分信息缺失、错误或模糊不清等问题。本文基于全国和地方植物志书及野外调查的植物性状信息,订正了中英文版维管植物的生长型和生活型性状,建立了中国维管植物生长型和生活型数据集。该数据集包含35,329种维管植物,内容包括植物名称、生长型和生活型性状及其数据来源,其中植物名称包括来自《中国植物志》、Flora of China和来自《中国生物物种名录(2023版)》的两个分类系统版本,以便读者相互对照不同分类系统的差异。依据茎木质化程度分为木本植物和草本植物两类,木本植物有12,600种,占35.7%;草本植物为22,729种,占64.3%。木本植物以中灌木生长型(2,917种,占23.2%)和常绿生活型(7,107种,占56.4%)为主,草本植物以杂类草生长型(19,900种,占87.6%)和多年生生活型(19,554种,占86.0%)为主。木本生长型中乔木物种以樟科和壳斗科占比较大,灌木物种以杜鹃花科、蔷薇科和豆科占比较大;常绿生活型以杜鹃花科占比较大,落叶生活型以蔷薇科占比较大。草本生长型中杂类草以菊科占比较大,禾草以禾本科占比较大;生活型中一年生、二年生和多年生分别以禾本科、紫草科和菊科占优势。本数据集涵盖了目前中国所有维管植物物种的生长型和生活型信息,可为植物性状研究提供数据基础。
文摘BACKGROUND Situs inversus totalis(SIT)is an extremely rare congenital malformation characterized by mirror displacement of the thoracoabdominal organs such as the heart,liver,spleen,and stomach.Herein,we describe a patient with SIT complicated with cholangiocarcinoma who underwent successful pancreaticoduodenectomy with the assistance of a da Vinci robot.CASE SUMMARY A 58-year-old female presented to the hospital with paroxysmal pain in her left upper abdomen,accompanied by jaundice and staining of the sclera as chief complaints.Imaging examination detected a mass at the distal end of the common bile duct,with inverted thoracic and abdominal organs.Endoscopic retrograde cholangiopancreatography forceps biopsy revealed the presence of a well-differentiated adenocarcinoma.The patient successfully underwent robotic-assisted pancreaticoduodenectomy;the operation lasted 300 min,the intraoperative blood loss was 500 mL,and there were no intraoperative and postoperative complications.CONCLUSION SIT is not directly related to the formation of cholangiocarcinoma.Detailed preoperative imaging examination is conducive to disease diagnosis and also convenient for determining the feasibility of tumor resection.Robot-assisted pancreaticoduodenectomy for SIT complicated with cholangiocarcinoma provides a safe,feasible,minimally invasive,and complication-free alternative with adequate preoperative planning combined with meticulous intraoperative procedures.
基金grants from the National Key Research and Development Program of China (No. 2016YFC0904100)National Natural Science Foundation of China (Nos. 81870460, 81570598, and 81370015)+3 种基金Science and Technology Innovation Action Plan of Shanghai Science and Technology Committee (No. 17441902200)Shanghai Municipal Education Commission, Gaofeng Clinical Medicine Grant (No. 20152207)Shanghai Jiao Tong University School of Medicine, MultiCenter Clinical Research Project (No. DLY201510)the Shanghai Health and Family Planning Committee Hundred Talents Program (No. 2018BR37).
文摘Background:Collagen type Ⅳ(COL4)-related nephropathy includes a variety of kidney diseases that occur with or without extra-renal manifestations caused by COL4A3-5 mutations.Previous studies revealed several novel mutations,including three COL4A3 missense mutations (G619R,G801R,and C1616Y) and the COL4A3 chr:228172489delA c.4317delA p.Thr1440ProfsX87 frameshift mutation that resulted in a truncated NC1 domain (hereafter named COL4A3 c.4317delA);however,the mutation mechanisms that lead to podocyte injury remain unclear.This study aimed to further explore the mutation mechanisms that lead to podocyte injury.Methods:Wild-type (WT) and four mutant COL4A3 segments were constructed into a lentiviral plasmid,then stably transfected into human podocytes.Real-time polymerase chain reaction and Western blotting were applied to detect endoplasmic reticulum stress (ERS)-and apoptosis-related mRNA and protein levels.Then,human podocytes were treated with MG132 (a proteasome inhibitor) and brefeldin A (a transport protein inhibitor).The human podocyte findings were verified by the establishment of a mus-Col4a3 knockout mouse monoclonal podocyte using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Casg) technology.Results:Our data showed that COL4A3 mRNA was significantly overexpressed in the lentivirus stably transfected podocytes.Moreover,the COL4A3 protein level was significantly increased in all groups except the COL4A3 c.4317delA group.Compared to the other test groups,the COL4A3 c.4317delA group showed excessive ERS and apoptosis.Podocytes treated with MG 132 showed remarkably increased intra-cellular expression of the COL4A3 c.4317delA mutation.MG132 intervention improved higher ERS and apoptosis levels in the COL4A3 c.4317delA group.Mouse monoclonal podocytes with COL4A3 chr:82717932insA c.4852insA p.Arg1618ThrfsX4 were successfully acquired;this NC1-truncated mutation suggested a higher level of ERS and relatively remarkable level of apoptosis compared to that of the WT
