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黄槿木材结构特征的分析 被引量:6
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作者 陈家宝 盛佳乐 +2 位作者 齐文玉 关鑫 林金国 《西北林学院学报》 CSCD 北大核心 2020年第1期257-260,267,共5页
借助连续变倍体视显微镜、生物数码显微镜和电镜观察黄槿木材的宏观、微观构造,按照许尔兹发进行解离,测量分析各种细胞的形态特征。结果表明,黄槿为散孔材,管孔主要为单管孔及径列复管孔,导管分子主要为圆柱形,单穿孔,椭圆形,穿孔板倾... 借助连续变倍体视显微镜、生物数码显微镜和电镜观察黄槿木材的宏观、微观构造,按照许尔兹发进行解离,测量分析各种细胞的形态特征。结果表明,黄槿为散孔材,管孔主要为单管孔及径列复管孔,导管分子主要为圆柱形,单穿孔,椭圆形,穿孔板倾斜,螺纹加厚缺如,无侵填体;管间纹孔互列,射线-导管间纹孔似管间纹孔;木纤维呈细长纺锤形,两端尖削,纤维长度417~1727μm,平均1092μm,属中等长度纤维;轴向薄壁组织肉眼下可见,主为环管束状及星散状;木射线肉眼下可见,射线组织异Ⅰ型及异Ⅱ型,宽2~4细胞,高5~47细胞,宽多为2~3细胞,高8~17细胞;瓦形细胞具翅子树型;轴向薄壁细胞和射线细胞皆含有晶体及颗粒状内含物,晶体多为晶簇状,少有菱形及方形。通过上述分析为黄槿木材的合理利用提供基础理论依据,丰富木材识别鉴定的基础理论。 展开更多
关键词 黄槿 瓦细胞 木材宏观构造 木材微观构造 扫描电子显微镜
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lncRNA DSCAM-AS1通过miR-144-5p/IRS2轴对甲状腺乳头状癌细胞的影响
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作者 吴天思 陈珊珊 +4 位作者 高博 石佳宝 管佳琪 张小宝 张立广 《中国现代普通外科进展》 CAS 2024年第3期173-176,共4页
目的:探究长链非编码RNA(lncRNA)唐氏综合征细胞黏附分子反义1(DSCAM-AS1)通过微小RNA(miR)-144-5p/胰岛素受体底物2(IRS2)轴促进甲状腺乳头状癌(PTC)细胞生长和侵袭的作用。方法:将PTC细胞株TPC-1随机分为对照组(Control组,完全培养基... 目的:探究长链非编码RNA(lncRNA)唐氏综合征细胞黏附分子反义1(DSCAM-AS1)通过微小RNA(miR)-144-5p/胰岛素受体底物2(IRS2)轴促进甲状腺乳头状癌(PTC)细胞生长和侵袭的作用。方法:将PTC细胞株TPC-1随机分为对照组(Control组,完全培养基正常培养)、si-NC组(转染si-NC)、si-DSCAM-AS1组(转染si-DSCAM-AS1)、si-DSCAM-AS1+inhibitor NC组(si-DSCAM-AS1与inhibitor NC共转染)、si-DSCAM-AS1+miR-144-5p inhibitor组(si-DSCAM-AS1与miR-144-5p inhibitor共转染)。RT-qPCR法检测DSCAM-AS1、miR-144-5p和IRS2 mRNA的表达;CCK-8法检测细胞增殖能力;Transwell实验检测细胞的侵袭能力;Western blot检测IRS2蛋白的表达。双荧光素酶报告基因实验验证靶向关系。结果:与Control组相比,si-DSCAM-AS1组TPC-1细胞DSCAM-AS1表达、吸光度(A450)值、细胞侵袭数目、IRS2表达显著降低(P<0.05),miR-144-5p表达显著升高(P<0.05)。与si-DSCAM-AS1组相比,si-DSCAM-AS1+miR-144-5p inhibitor组A450值、细胞侵袭数目、IRS2表达显著升高(P<0.05),miR-144-5p表达显著降低(P<0.05)。DSCAM-AS1靶向负调控miR-144-5p表达,miR-144-5p靶向负调控IRS2表达。结论:沉默DSCAM-AS1可能通过上调miR-144-5p来抑制IRS2蛋白的表达,从而抑制PTC细胞生长和侵袭。 展开更多
关键词 长链非编码RNA 唐氏综合征细胞黏附分子反义1 miR-144-5p 胰岛素受体底物2 甲状腺乳头状癌 侵袭
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Beam dynamics study of the photoinjector at Wuhan advanced light source
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作者 Ze-Yi Dai Yuan-Cun Nie +8 位作者 jian-Hua Zhong Lan-Xin Liu Zi-Shuo Liu jia-bao guan Ji-Ke Wang Yuan Chen Ye Zou Hao-Hu Li jian-Hua He 《Radiation Detection Technology and Methods》 CSCD 2024年第3期1319-1332,共14页
Background A full-energy LINAC is under development at Wuhan Advanced Light Source(WALS)to provide beams for the 1.5 GeV storage ring proposed as a fourth-generation synchrotron radiation light source and a future fre... Background A full-energy LINAC is under development at Wuhan Advanced Light Source(WALS)to provide beams for the 1.5 GeV storage ring proposed as a fourth-generation synchrotron radiation light source and a future free electron laser(FEL)facility.The LINAC starts from a photoinjector that is able to produce electron beams with low emittance(<1 mm·mrad),small relative energy spread(<0.5%),and high bunch charge(~1 nC).Purpose and methods To minimize the output emittance and RMS bunch length for the purpose of improving the photoinjector brightness,the influence of different laser pulse profiles on the slice emittance is investigated,and the beam dynamics simulation is performed with non-dominated sorting genetic algorithm-II(NSGA-II)combining with ASTRA code to find the optimal solution at a bunch charge of 1 nC.Results and conclusion In this paper,the beam dynamics optimization of the photoinjector is presented in detail;the simulation results imply that the laser pulse profile with 1σtruncated Gaussian distribution in transverse and flat-top-like temporal distribution is beneficial to the improvement of beam brightness of the photoinjector,which shows an output emittance of 0.63 mm·mrad in the case of intrinsic thermal emittance of 0.43 mm·mrad. 展开更多
关键词 PHOTOINJECTOR Beam dynamics optimization Beam emittance Driving laser NSGA-II algorithm
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人参果提取物对缺氧复氧诱导HUVEC细胞损伤的保护作用 被引量:1
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作者 李丽静 周凯旋 +7 位作者 薛嘉宝 刘佳 关婉辰 石美玲 王月洁 马晓彤 宋嘉昱 鲍慧玮 《人参研究》 2020年第6期6-9,共4页
目的研究人参果总皂昔对缺氧复氧诱导人脐静脉内皮细胞(HUVEC细胞)损伤的保护作用。方法体外培养的HUVEC细胞分为正常组、模型组、参麦注射液组、人参果总皂昔低、中、高剂量组(25、50、100μg/ml),缺氧复氧处理细胞,采用CCK8法检测细... 目的研究人参果总皂昔对缺氧复氧诱导人脐静脉内皮细胞(HUVEC细胞)损伤的保护作用。方法体外培养的HUVEC细胞分为正常组、模型组、参麦注射液组、人参果总皂昔低、中、高剂量组(25、50、100μg/ml),缺氧复氧处理细胞,采用CCK8法检测细胞活力;倒置显微镜下检测细胞形态,MDC荧光染色观察自噬;检测丙二醛(MDA)、乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)含量。结果造模后与正常对照组比较,模型组细胞部分皱缩,生长受到抑制,细胞活力减弱,MDC染色显示自噬增加,MDA、LDH升高,SOD降低,与模型组比较,人参果总皂昔使细胞损伤程度减轻,细胞增殖率增加,细胞自噬减轻,SOD升高,MDA、LDH减少。结论人参果总皂昔对缺氧复氧的HUVEC细胞具有保护作用,可能通过提高细胞抗氧化能力,降低自噬水平发挥作用。 展开更多
关键词 人参果总皂昔 HUVEC 缺氧复氧 自噬
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Design of S-band photoinjector with high bunch charge and low emittance based on multi-objective genetic algorithm 被引量:1
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作者 Ze-Yi Dai Yuan-Cun Nie +9 位作者 Zi Hui Lan-Xin Liu Zi-Shuo Liu jian-Hua Zhong jia-bao guan Ji-Ke Wang Yuan Chen Ye Zou Hao-Hu Li jian-Hua He 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第3期93-105,共13页
High-brightness electron beams are required to drive LINAC-based free-electron lasers(FELs)and storage-ring-based synchrotron radiation light sources.The bunch charge and RMS bunch length at the exit of the LINAC play... High-brightness electron beams are required to drive LINAC-based free-electron lasers(FELs)and storage-ring-based synchrotron radiation light sources.The bunch charge and RMS bunch length at the exit of the LINAC play a crucial role in the peak current;the minimum transverse emittance is mainly determined by the injector of the LINAC.Thus,a photoin-jector with a high bunch charge and low emittance that can simultaneously provide high-quality beams for 4th generation synchrotron radiation sources and FELs is desirable.The design of a 1.6-cell S-band 2998-MHz RF gun and beam dynamics optimization of a relevant beamline are presented in this paper.Beam dynamics simulations were performed by combining ASTRA and the multi-objective genetic algorithm NSGA II.The effects of the laser pulse shape,half-cell length of the RF gun,and RF parameters on the output beam quality were analyzed and compared.The normalized transverse emittance was optimized to be as low as 0.65 and 0.92 mm·mrad when the bunch charge was as high as 1 and 2 nC,respectively.Finally,the beam stability properties of the photoinjector,considering misalignment and RF jitter,were simulated and analyzed. 展开更多
关键词 Electron linear accelerator PHOTOINJECTOR Beam dynamics Multi-objective genetic algorithm
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Design and optimization of diffraction-limited storage ring lattices based on many-objective evolutionary algorithms 被引量:1
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作者 He-Xing Yin jia-bao guan +1 位作者 Shun-Qiang Tian Ji-Ke Wang 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第10期20-35,共16页
Multi-objective evolutionary algorithms(MOEAs) are typically used to optimize two or three objectives in the accelerator field and perform well. However, the performance of these algorithms may severely deteriorate wh... Multi-objective evolutionary algorithms(MOEAs) are typically used to optimize two or three objectives in the accelerator field and perform well. However, the performance of these algorithms may severely deteriorate when the optimization objectives for an accelerator are equal to or greater than four. Recently, many-objective evolutionary algorithms(MaOEAs)that can solve problems with four or more optimization objectives have received extensive attention. In this study, two diffraction-limited storage ring(DLSR) lattices of the Extremely Brilliant Source(ESRF-EBS) type with different energies were designed and optimized using three MaOEAs and a widely used MOEA. The initial population was found to have a significant impact on the performance of the algorithms and was carefully studied. The performances of the four algorithms were compared, and the results demonstrated that the grid-based evolutionary algorithm(GrEA) had the best performance.Ma OEAs were applied in many-objective optimization of DLSR lattices for the first time, and lattices with natural emittances of 116 and 23 pm·rad were obtained at energies of 2 and 6 GeV, respectively, both with reasonable dynamic aperture and local momentum aperture(LMA). This work provides a valuable reference for future many-objective optimization of DLSRs. 展开更多
关键词 Storage ring lattices Many-objective evolutionary algorithms GrEA algorithm NSGA
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银鲳早期鳞被的覆盖和初生鳞的发育
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作者 唐杰 周硕 +5 位作者 王亚军 胡佳宝 王想兵 王冠林 蒋欢 严小军 《水生生物学报》 CAS CSCD 北大核心 2023年第12期1948-1953,共6页
为了研究人工养殖银鲳早期鳞被的覆盖和初生鳞的发育过程,采用茜素红染色法和苏木精-伊红染色法对人工养殖银鲳的鳞片进行了观察和测量。结果显示:银鲳鳞片的起始中心有3个,分别为尾柄中部区域、鳃盖后缘和胸鳍基部;在(20±1.8)℃... 为了研究人工养殖银鲳早期鳞被的覆盖和初生鳞的发育过程,采用茜素红染色法和苏木精-伊红染色法对人工养殖银鲳的鳞片进行了观察和测量。结果显示:银鲳鳞片的起始中心有3个,分别为尾柄中部区域、鳃盖后缘和胸鳍基部;在(20±1.8)℃的育苗水体中,银鲳在体长(30±0.95)mm开始出现鳞片,鳃盖后缘及胸鳍基部的鳞片沿着侧线方向扩展,直至与尾柄中心向侧线方向扩展的鳞片相接;在体长(45.58±0.73)mm鳞被覆盖完全。鳞被向头部扩展的速度快于向尾部扩展的速度,向背部扩展的速度快于向腹部扩展的速度。银鲳整个生命阶段都为圆鳞,不存在圆鳞-栉鳞的过渡。组织学结果显示:鳞片是由鱼类表皮和真皮之间的成纤维细胞聚集发育而来的,其过程共包括形态发生早期、形态发生晚期、分化早期和分化晚期4个阶段;银鲳鳞片的发生在变态(由梭形过渡到成年侧扁状外形)之后,且各鳍条均已完全形成,与其他真骨鱼具有共性。这些研究结果丰富了银鲳早期发育的生物学数据,有助于了解其在系统学和功能形态学上的特征。 展开更多
关键词 鳞被 覆盖 初生鳞 发育 银鲳
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