Objective:To observe the effects of electroacupuncture(EA)on type 2 innate lymphoid cell(ILC2)function in white adipose tissue(WAT)of high-fat diet(HFD)-induced obese rats to reveal the mechanism underlying weight los...Objective:To observe the effects of electroacupuncture(EA)on type 2 innate lymphoid cell(ILC2)function in white adipose tissue(WAT)of high-fat diet(HFD)-induced obese rats to reveal the mechanism underlying weight loss.Methods:Male Winstar rats(n=15)were randomly divided into control,HFD,and HFD+EA groups,with five rats in each group.An obesity model was established by feeding rats with a HFD.EA(2 Hz,1 mA)was applied to"Zhongwan(CV12)"bilateral"Tianshu(ST25)""Zusanli(ST36)"and"Sanyinjiao(SP6)"for15 min,7 days a week,for a total of 5 weeks.During EA treatment,the HFD group was fed a HFD,whereas rats in the control group were maintained on a normal diet.There was no intervention in either the control or the HFD groups.Body weight was measured weekly.Full-field adipocyte morphology,adipocyte volume,and the number of abdominal WAT were observed under a microscope after sectioning and hematoxylin and eosin staining.The levels of IL-5(interleukin-5),IL-13(interleukin-13),IL-33(interleukin-33),and ST2(growth stimulating gene 2 protein)in the WAT were assayed using enzymelinked immunosorbent assay and reverse transcription-polymerase chain reaction(RT-PCR).The expression levels of browning markers including PGClα(peroxisome proliferator-activated receptor gamma coactivator 1-alpha),PPARγ(peroxisome proliferator-activated receptor gamma),PRDM16(histone-lysine Nmethyltransferase),and UCP1(uncoupling protein 1 in WAT were detected by western blot analysis and RT-PCR.Results:After a 7-week induction period,rats in both the HFD and HFD+EA groups were 20%heavier than their initial weights before modeling(P<0.01,P<0.001).Following a 5-week EA intervention,the body mass and Lee index of obese rats were significantly reduced(P<0.05).The HE results showed that adipocytes in the HFD group displayed a significant increase in diameter and a decrease in number compared to the control group.However,in the HFD+EA group,white adipocytes exhibited reduced diameter and appeared more regular in shape.In the control group,the proportion of展开更多
基金Supported by the State Key Laboratory of Dampness Syndrome of Chinese Medicine Special Fund:SZ2021ZZ08the Guangdong Provincial Bureau of Chinese Medicine:20233013the Guangdong Provincial Hospital of Chinese Medicine Fund:YN2020MS02,YN2019ZWB01,YN2019ML01。
文摘Objective:To observe the effects of electroacupuncture(EA)on type 2 innate lymphoid cell(ILC2)function in white adipose tissue(WAT)of high-fat diet(HFD)-induced obese rats to reveal the mechanism underlying weight loss.Methods:Male Winstar rats(n=15)were randomly divided into control,HFD,and HFD+EA groups,with five rats in each group.An obesity model was established by feeding rats with a HFD.EA(2 Hz,1 mA)was applied to"Zhongwan(CV12)"bilateral"Tianshu(ST25)""Zusanli(ST36)"and"Sanyinjiao(SP6)"for15 min,7 days a week,for a total of 5 weeks.During EA treatment,the HFD group was fed a HFD,whereas rats in the control group were maintained on a normal diet.There was no intervention in either the control or the HFD groups.Body weight was measured weekly.Full-field adipocyte morphology,adipocyte volume,and the number of abdominal WAT were observed under a microscope after sectioning and hematoxylin and eosin staining.The levels of IL-5(interleukin-5),IL-13(interleukin-13),IL-33(interleukin-33),and ST2(growth stimulating gene 2 protein)in the WAT were assayed using enzymelinked immunosorbent assay and reverse transcription-polymerase chain reaction(RT-PCR).The expression levels of browning markers including PGClα(peroxisome proliferator-activated receptor gamma coactivator 1-alpha),PPARγ(peroxisome proliferator-activated receptor gamma),PRDM16(histone-lysine Nmethyltransferase),and UCP1(uncoupling protein 1 in WAT were detected by western blot analysis and RT-PCR.Results:After a 7-week induction period,rats in both the HFD and HFD+EA groups were 20%heavier than their initial weights before modeling(P<0.01,P<0.001).Following a 5-week EA intervention,the body mass and Lee index of obese rats were significantly reduced(P<0.05).The HE results showed that adipocytes in the HFD group displayed a significant increase in diameter and a decrease in number compared to the control group.However,in the HFD+EA group,white adipocytes exhibited reduced diameter and appeared more regular in shape.In the control group,the proportion of
