The modulation of dielectric anisotropy(△ε)is pivotal for elucidating molecular interactions and directing the alignment of liquid crystals.In this study,we combine liquid crystals with opposing dielectric anisotrop...The modulation of dielectric anisotropy(△ε)is pivotal for elucidating molecular interactions and directing the alignment of liquid crystals.In this study,we combine liquid crystals with opposing dielectric anisotropies to explore the impact of varying concentrations on their properties.We report the sign-reversal of△εin both the nematic and smectic A phases of these mixed liquid crystals,alongside a dual-frequency behaviour across a broad temperature spectrum.Our research further quantifies the influence of mixture ratios under various temperatures and electric field frequencies.This exploration may pave the way for the discovery of new physical phenomena.展开更多
In this study, the effect of icariin, a flavonoid from the Chinese traditional medicine epimedium, on miRNA-21 of mouse developmental blastocysts in vitro and the development of preimplantation embryos were studied. T...In this study, the effect of icariin, a flavonoid from the Chinese traditional medicine epimedium, on miRNA-21 of mouse developmental blastocysts in vitro and the development of preimplantation embryos were studied. The possible effective targets of icariin promoting preimplantation embryo development in vitro and anti-apoptosis were determined. The embryos were cultured in modified CZB medium （mCZB） as control group. The experimental group （Ica group） was supplemented with 0.6 μg mL-1 icariin. Mouse pronuclear embryos were cultured in vitro until blastocysts. The development rates of preimplantation embryos were observed. The total cell number, apoptotic cell number and the rate of apoptotic cells in blastocysts were analysed by the staining of Hoechst33342 and labeling of TUNEL and detected under a laser confocal scanning microscope. The miRNA-21 expression, the mRNA levels of pro-apoptotic Caspase3, and the target genes of miRNA-21： pro-apoptotic PTEN, anti-apoptotic Bcl-2 were detected by real-time RT-PCR. The results showed that percentages of morulaes and blastocysts in Ica group were both extremely higher than control group （（85.14±6.57）% vs. （72.04±11.58）%; （82.50±7.11）% vs. （66.80±11.70）%, respectively, P〈0.01）. The total cell number ofblastocysts had extreme difference between Ica group and control group （（61.40±9.64） vs. （46.23±4.50）, P〈0.01）. The apoptotic cell number and rate of apoptotic cells of blastocysts were both reduced in Ica group （（1.47±0.51） vs. （2.94±0.66）; （2.40±0.27）% vs. （6.25±0.62）%, respectively, P〈0.01）. Compared to control group, addition of icariin into mCZB extremely increased the expression of anti-apoptotic miRNA-21 （P〈0.01）, down-regulated pro-apoptotic Caspase3 （P〈0.05） and PTEN （P〈0.01）, up-regulated anti-apoptotic Bcl-2 （P〈0.01）. In conclusion, icariin could reduce the apoptosis, promote the embryo development in vitro by enhancing miRNA-21 expression to up-regulated anti-展开更多
目的系统评价肺癌中医临床疗效评估工具的测量学特性和相关研究的方法学质量,为医护人员选择高质量的工具提供循证依据。方法系统检索PubMed、The Cochrane Library、Web of Science、Embase、PsycINFO、CINAHL、中国生物医学文献数据...目的系统评价肺癌中医临床疗效评估工具的测量学特性和相关研究的方法学质量,为医护人员选择高质量的工具提供循证依据。方法系统检索PubMed、The Cochrane Library、Web of Science、Embase、PsycINFO、CINAHL、中国生物医学文献数据库、中国知网、万方数据库、维普数据库,检索时限为建库至2023年6月。分别采用基于共识的健康测量工具遴选标准(Consensus-based Standards for the Selection of Health Measurement Instruments,COSMIN)偏倚风险清单和量表测量学性能评价标准评定相关工具的方法学质量及测量属性并形成最终推荐意见。结果最终纳入8个肺癌中医临床疗效评估工具,均未报告测量误差、跨文化效度或测量等同性。结论现有证据提示肺癌中医临床疗效评估工具测量学特性评价不够全面,总体研究方法学质量偏低,其中基于中医理论的恶性肿瘤生活质量评价体系之肺癌量表(Quality of Life Assessment System for Cancer based on Traditional Chinese Medicine-Lung Cancer,QLASTCM-Lu)研究质量及性能评价尚可,未来应进一步完善和验证现有工具,或重新构建低偏倚风险、高适用性的肺癌中医临床疗效测量工具。展开更多
基金Project supported by the National Key Research and Development Program of China (Grant No.2022YFA1405000)the National Natural Science Foundation of China (Grant No.62375141)+1 种基金the Natural Science Foundation of Jiangsu Province,Major Project (Grant No.BK20212004)the Natural Science Research Start-up Foundation of Recruiting Talents of Nanjing University of Posts and Telecommunications (Grant Nos.NY222122 and NY222105)。
文摘The modulation of dielectric anisotropy(△ε)is pivotal for elucidating molecular interactions and directing the alignment of liquid crystals.In this study,we combine liquid crystals with opposing dielectric anisotropies to explore the impact of varying concentrations on their properties.We report the sign-reversal of△εin both the nematic and smectic A phases of these mixed liquid crystals,alongside a dual-frequency behaviour across a broad temperature spectrum.Our research further quantifies the influence of mixture ratios under various temperatures and electric field frequencies.This exploration may pave the way for the discovery of new physical phenomena.
基金supported by the Beijing Natural Science Foundation of China (6112004)the High Quality Paper Support Project of Beijing University of Agriculture, China (GL2012006)
文摘In this study, the effect of icariin, a flavonoid from the Chinese traditional medicine epimedium, on miRNA-21 of mouse developmental blastocysts in vitro and the development of preimplantation embryos were studied. The possible effective targets of icariin promoting preimplantation embryo development in vitro and anti-apoptosis were determined. The embryos were cultured in modified CZB medium （mCZB） as control group. The experimental group （Ica group） was supplemented with 0.6 μg mL-1 icariin. Mouse pronuclear embryos were cultured in vitro until blastocysts. The development rates of preimplantation embryos were observed. The total cell number, apoptotic cell number and the rate of apoptotic cells in blastocysts were analysed by the staining of Hoechst33342 and labeling of TUNEL and detected under a laser confocal scanning microscope. The miRNA-21 expression, the mRNA levels of pro-apoptotic Caspase3, and the target genes of miRNA-21： pro-apoptotic PTEN, anti-apoptotic Bcl-2 were detected by real-time RT-PCR. The results showed that percentages of morulaes and blastocysts in Ica group were both extremely higher than control group （（85.14±6.57）% vs. （72.04±11.58）%; （82.50±7.11）% vs. （66.80±11.70）%, respectively, P〈0.01）. The total cell number ofblastocysts had extreme difference between Ica group and control group （（61.40±9.64） vs. （46.23±4.50）, P〈0.01）. The apoptotic cell number and rate of apoptotic cells of blastocysts were both reduced in Ica group （（1.47±0.51） vs. （2.94±0.66）; （2.40±0.27）% vs. （6.25±0.62）%, respectively, P〈0.01）. Compared to control group, addition of icariin into mCZB extremely increased the expression of anti-apoptotic miRNA-21 （P〈0.01）, down-regulated pro-apoptotic Caspase3 （P〈0.05） and PTEN （P〈0.01）, up-regulated anti-apoptotic Bcl-2 （P〈0.01）. In conclusion, icariin could reduce the apoptosis, promote the embryo development in vitro by enhancing miRNA-21 expression to up-regulated anti-
文摘目的系统评价肺癌中医临床疗效评估工具的测量学特性和相关研究的方法学质量,为医护人员选择高质量的工具提供循证依据。方法系统检索PubMed、The Cochrane Library、Web of Science、Embase、PsycINFO、CINAHL、中国生物医学文献数据库、中国知网、万方数据库、维普数据库,检索时限为建库至2023年6月。分别采用基于共识的健康测量工具遴选标准(Consensus-based Standards for the Selection of Health Measurement Instruments,COSMIN)偏倚风险清单和量表测量学性能评价标准评定相关工具的方法学质量及测量属性并形成最终推荐意见。结果最终纳入8个肺癌中医临床疗效评估工具,均未报告测量误差、跨文化效度或测量等同性。结论现有证据提示肺癌中医临床疗效评估工具测量学特性评价不够全面,总体研究方法学质量偏低,其中基于中医理论的恶性肿瘤生活质量评价体系之肺癌量表(Quality of Life Assessment System for Cancer based on Traditional Chinese Medicine-Lung Cancer,QLASTCM-Lu)研究质量及性能评价尚可,未来应进一步完善和验证现有工具,或重新构建低偏倚风险、高适用性的肺癌中医临床疗效测量工具。
