Regulation of mitochondrial biogenesis is essential for proper cellular functioning. Mitochondrial DNA （mtDNA） depletion and the resulting mitochondrial malfunction have been implicated in cancer, neurodegeneration,...Regulation of mitochondrial biogenesis is essential for proper cellular functioning. Mitochondrial DNA （mtDNA） depletion and the resulting mitochondrial malfunction have been implicated in cancer, neurodegeneration, diabetes, aging, and many other human diseases. Although it is known that the dynamics of the mammalian mitochondrial genome are not linked with that of the nuclear genome, very little is known about the mechanism of mtDNA propagation. Nevertheless, our understanding of the mode of mtDNA replication has ad- vanced in recent years, though not without some controversies. This review summarizes our current knowledge of mtDNA copy number control in mammalian cells, while focusing on both mtDNA replication and turnover. Although mtDNA copy number is seemingly in excess, we reason that mtDNA copy number control is an important aspect of mitochondrial genetics and biogenesis and is essential for normal cellular function.展开更多
Soft magnetic metallic materials have been widely used for absorbing electromagnetic wave.Flaky Fe 86 t Si t Al 14（t=9,11,14 and 16） alloys powders were prepared from melt-quenched ribbons by annealing and milling.I...Soft magnetic metallic materials have been widely used for absorbing electromagnetic wave.Flaky Fe 86 t Si t Al 14（t=9,11,14 and 16） alloys powders were prepared from melt-quenched ribbons by annealing and milling.In a previous report we discussed the order-disordered structure of this alloys.In this article,we studied their electron structure.Covalence electron numbers of（111） and（100） plane increase with increasing Si content but Bohr magneton decreases.Complex permittivity and complex permeability are both decreased with increasing Si content t.The lowest value of reflectivity among the four alloys is originated from Fe 70 Si 14 Al 14.The peak values of reflectivity are all lower than 10 dB,and the absorbing frequency range（R 10 dB） increases from 1 to 2 GHz when t reaches 16.展开更多
The feature of reinforcing bars is introduced into dynamic cavity-expansion theory. Based on the elastic-plastic response penetration model of plain (i.e., unreinforced) concrete (Forrestal and Tzou, 1997), a dynamic ...The feature of reinforcing bars is introduced into dynamic cavity-expansion theory. Based on the elastic-plastic response penetration model of plain (i.e., unreinforced) concrete (Forrestal and Tzou, 1997), a dynamic spherical cavity-expansion penetration model for reinforced-concrete targets is developed with consideration of the circumferential restriction effect derived from reinforcing bars in the crushed region. The theoretical solution and simplified calculation formula for the cavity radial stress in incompressible and compressible reinforced concrete are obtained by introducing a reinforcement ratio as the volume fraction of rebars in the concrete target. A damping function is presented to describe the restriction effect of a single layer of reinforcing bars on the surrounding concrete, thus establishing a model to calculate the penetration resistance of multilayer reinforced-concrete targets. Compared with test data for the penetration depth, this model considering the circumferential restriction effect produces better results compared with the existing theory.展开更多
In this study,with the meso-scale model reliably validated in our previous work(Construction and Building Materials,2018),the waveform features of plain concrete under various loading conditions and especially with co...In this study,with the meso-scale model reliably validated in our previous work(Construction and Building Materials,2018),the waveform features of plain concrete under various loading conditions and especially with considering stress non-equilibrium are reliably reproduced and predicted.Associating with waveform features,the violation indicator of the specimen stress equilibrium in the split Hopkinson pressure bar test is identified for concrete-like damage softening materi-als.The concrete material behaviors for stress non-equilibrium are further analyzed,e.g.the dynamic increase factor(DIF)and damage development,etc.The conception of“damage failure volume”is introduced,and a new method of defining the development of concrete dynamic damage is given in the nimierical study.What’s more,the“compression wave”and“double peak”phenomena observed in the experiment are further interpreted based on the means of numerical simulation.Waveform features how to reflect the concrete material properties is also concluded.The results show that,the disappearance of the“double peak” phenomenon of reflection curve under high strain rate can be regarded as the indicator of the violation of stress equilibrium.After the violation of the stress equilibrium,the relevant DIFs of the concrete specimen will not change significantly.Especially,the concrete specimen will turn into structural response from material response.The conception of“damage failure volume”can well explain the generation of the“double peak”phenomenon of the reflection curve.The “compression wave” phenomenon of reflection curve under lower strain rates is derived from the unloading expansion recovery of the concrete specimen.Furthermore,under the same loading condition,the amplitude of the first peak of the reflection curve can be used as the evaluation standard of the bonding quality between mortar and aggregates.展开更多
Rockbursts are sudden and violent rock failures that can lead to huge production and equipment losses,injury or death of mining workers.Buckling has been regarded as one of the key mechanisms of rockbursts,which are o...Rockbursts are sudden and violent rock failures that can lead to huge production and equipment losses,injury or death of mining workers.Buckling has been regarded as one of the key mechanisms of rockbursts,which are often induced by dynamic loads from mining excavations,such as drilling and blasting in underground mining.The paper attempts to investigate the dynamic buckling mechanism of pillar rockbursts in underground mining,by considering rockbursts as a dynamic stability problem of underground rock structures.The results include:(1)A new explanation of the“sudden and violent”phenomenon of rockbursts,characterized by exponential growth of the amplitudes of transverse displacement responses,even in the presence of rock damping;(2)Identification of the critical role in inducing rockbursts of dynamic loads that bear frequencies approximately double the natural pillar frequency;(3)The greater influence on rockburst occurrence of the amplitude of dynamic component relative to the static component of loads;and(4)Quantification of the relative effects of stress waveform of dynamic loads on pillar rockbursts,which are in decreasing order if other parameters remain constant:rectangular,sinusoidal,and exponential waveforms.Application examples are provided and limitations of the approach are discussed.This research is motivated by the on-going and ubiquitous occurrence of rockbursts in underground excavations all around the world.In contrast to conventional methods that use rock specimens or rock materials to study rockbursts,this investigation emphasizes the structural effects on rockbursts,which has potential applications in hard rock mining engineering.展开更多
Thelow cyclefatigue( LCF) propertiesof Ti 2 Al 2 5 Zralloytubeand rod specimens weredetermined at R.T. Thestress～strain curve equation under cyclic and monotonicloading andtherelation of totalstrain vsfatiguelife...Thelow cyclefatigue( LCF) propertiesof Ti 2 Al 2 5 Zralloytubeand rod specimens weredetermined at R.T. Thestress～strain curve equation under cyclic and monotonicloading andtherelation of totalstrain vsfatiguelife of Ti 2 Al 2 5Zr alloy were established. Theeffectsofstrain ratio R,strain amplitudeεaandspecimenshapeoncyclicdeformingbehavior and fatigueproperties were analyzed . The fatiguefracture features were also discussed ac cording as SEMobservation. The resultsshow , at R = 0 1 ,thecyclicstress straincurvesof Ti 2 Al 2 5Zr alloytube and rodspecimensappear ascyclesoftening. When Ris 1 ,itsrodspecimen behavesascyclesoftening;atlow εavalues; whereasitbehavesascyclehardeningat high εa values. The LCFproperties determined withtubespecimen islower apparentlythanthat withrod one. Thereare muchirregularrecrystallineconvexin LCFfracturesof Ti 2 Al 2 5Zr alloytubeand rodspecimens, and muchsecondarycracks,fatiguestriationsand holes alsoexistamongthem .展开更多
Bajiaolian, one of the species in the Mayapple family ( Podophyllum pelatum ), has been widely used as a traditional Chinese herbal medicine for the remedies of snake bites, general weakness, poisons, condyloma accumi...Bajiaolian, one of the species in the Mayapple family ( Podophyllum pelatum ), has been widely used as a traditional Chinese herbal medicine for the remedies of snake bites, general weakness, poisons, condyloma accuminata, lymphadenopathy, and certain tumors in China. In Western medicine, Podophyllum was first used medically as a laxative in the early 19th century. Since 1940, the resin of podophyllum has also been used topically for various skin lesions, such as warts and condyloma. Human poisonings have been reported.An animal model was established to investigate the neurotoxic effects of Bajiaolian. Podophyllotoxin, the major active ingredient in Podophyllum, was injected (ip) to young adult male rats at doses of 0, 5, 10, or 15 mg-kg-1 b.w.. The animals were sacrificed 72 h after injection.Neuronal changes were readily observable in animals treated with 10 or 15 mg-kg-1 of the toxin. Edematous changes of the anterior horn motoneurons were observed in the spinal cord. No neuronal necrosis was found. The type of neuronal swelling is believed to be only a transient change and would probably subside with time if no further assaults occur. More serious and perhaps longer term of changes were found in the dorsal ganglion neurons and the nerve fibers (axons) in the central and peripheral nervous system. Severe depletion of the Nissl substance (RNA/polyribosomes) was observed in the dorsal root ganglion neurons. Alterations in these sensory neurons would give rise to and correlate with the sensory disturbances experienced by the patients. Bodian staining also revealed a dose-related increase in the coarseness (thickness) of the nerve fibers (axons) in the cerebellum, cerebral cortex, brainstem, and spinal cord.This is the first scientific study showing the neurotoxicity of Bajiaolian, a commonly used Chinese herbal medicine. Toxicities on other organ systems by this drug certainly exist. Caution should be exercised in the dispensing and usage of this medicine.展开更多
Young male rats were orally intubated with podophyllotoxin: Group I, control animals, orally fed with vehicle only; Group Ⅱ, fed with an initial dose of 5 mg.kg-1 b.w., followed by a daily dose of 1.67 mg-kg-1 b.w. f...Young male rats were orally intubated with podophyllotoxin: Group I, control animals, orally fed with vehicle only; Group Ⅱ, fed with an initial dose of 5 mg.kg-1 b.w., followed by a daily dose of 1.67 mg-kg-1 b.w. for 7d. Group III, fed with an initial dose of 15 mg.kg-1 b.w., followed by a daily dose of 5 mg.kg-1 b.w. for 7d. All animals were sacrificed 72 h after the last dosing.Histopathological examination revealed dose-related fatty change of the liver, atrophy andi degenerative changes of the intestinal epithelial linings and testicular seminiferous tubules. Depletion of the pancreatic acinar cell granules was also apparent in the Group III animals. No pathology, however, was observed in the kidneys. The present study demonstrated for the first time degenerative changes in the liver, intestine, testis, and pancreas of animals ingested podophyllotoxin. These pathological changes correlate well with the clinical signs/symptoms of abnormal liver function, abdominal pain and diarrhea, and reduced serum amylase in humans poisonded by podophyllum. Inhibition of protein synthesis and mitosis (disruption of microtubules) are believed to be the underlying mechanisms of these changes observed in the animals intoxicated by. podophyllotoxin.展开更多
基金supported by the National Institute of Aging/National Institution of Health, USA (No. AG025223 and AG024640) to YB.
文摘Regulation of mitochondrial biogenesis is essential for proper cellular functioning. Mitochondrial DNA （mtDNA） depletion and the resulting mitochondrial malfunction have been implicated in cancer, neurodegeneration, diabetes, aging, and many other human diseases. Although it is known that the dynamics of the mammalian mitochondrial genome are not linked with that of the nuclear genome, very little is known about the mechanism of mtDNA propagation. Nevertheless, our understanding of the mode of mtDNA replication has ad- vanced in recent years, though not without some controversies. This review summarizes our current knowledge of mtDNA copy number control in mammalian cells, while focusing on both mtDNA replication and turnover. Although mtDNA copy number is seemingly in excess, we reason that mtDNA copy number control is an important aspect of mitochondrial genetics and biogenesis and is essential for normal cellular function.
基金State Key Laboratory of Electronic Thin Films and Integrated Devices for support of this work (KFJJ200815)supported by the Scientific Research Fund of Sichuan Provincial Education Department,China (08ZA026)Talents Project of Xihua University,China (R1020106)
文摘Soft magnetic metallic materials have been widely used for absorbing electromagnetic wave.Flaky Fe 86 t Si t Al 14（t=9,11,14 and 16） alloys powders were prepared from melt-quenched ribbons by annealing and milling.In a previous report we discussed the order-disordered structure of this alloys.In this article,we studied their electron structure.Covalence electron numbers of（111） and（100） plane increase with increasing Si content but Bohr magneton decreases.Complex permittivity and complex permeability are both decreased with increasing Si content t.The lowest value of reflectivity among the four alloys is originated from Fe 70 Si 14 Al 14.The peak values of reflectivity are all lower than 10 dB,and the absorbing frequency range（R 10 dB） increases from 1 to 2 GHz when t reaches 16.
基金the National Outstanding Young Scientist Foundation of China (Grant 11225213)the National Natural Science Foundation of China (Grants 11390361 and 11390362).
文摘The feature of reinforcing bars is introduced into dynamic cavity-expansion theory. Based on the elastic-plastic response penetration model of plain (i.e., unreinforced) concrete (Forrestal and Tzou, 1997), a dynamic spherical cavity-expansion penetration model for reinforced-concrete targets is developed with consideration of the circumferential restriction effect derived from reinforcing bars in the crushed region. The theoretical solution and simplified calculation formula for the cavity radial stress in incompressible and compressible reinforced concrete are obtained by introducing a reinforcement ratio as the volume fraction of rebars in the concrete target. A damping function is presented to describe the restriction effect of a single layer of reinforcing bars on the surrounding concrete, thus establishing a model to calculate the penetration resistance of multilayer reinforced-concrete targets. Compared with test data for the penetration depth, this model considering the circumferential restriction effect produces better results compared with the existing theory.
基金supported by the National Natural Science Foundations of China(Grants 11390361,11627901,and 11872118).
文摘In this study,with the meso-scale model reliably validated in our previous work(Construction and Building Materials,2018),the waveform features of plain concrete under various loading conditions and especially with considering stress non-equilibrium are reliably reproduced and predicted.Associating with waveform features,the violation indicator of the specimen stress equilibrium in the split Hopkinson pressure bar test is identified for concrete-like damage softening materi-als.The concrete material behaviors for stress non-equilibrium are further analyzed,e.g.the dynamic increase factor(DIF)and damage development,etc.The conception of“damage failure volume”is introduced,and a new method of defining the development of concrete dynamic damage is given in the nimierical study.What’s more,the“compression wave”and“double peak”phenomena observed in the experiment are further interpreted based on the means of numerical simulation.Waveform features how to reflect the concrete material properties is also concluded.The results show that,the disappearance of the“double peak” phenomenon of reflection curve under high strain rate can be regarded as the indicator of the violation of stress equilibrium.After the violation of the stress equilibrium,the relevant DIFs of the concrete specimen will not change significantly.Especially,the concrete specimen will turn into structural response from material response.The conception of“damage failure volume”can well explain the generation of the“double peak”phenomenon of the reflection curve.The “compression wave” phenomenon of reflection curve under lower strain rates is derived from the unloading expansion recovery of the concrete specimen.Furthermore,under the same loading condition,the amplitude of the first peak of the reflection curve can be used as the evaluation standard of the bonding quality between mortar and aggregates.
基金supported,in part,by the Natural Sciences and Engineering Research Council of Canada(NSERC)
文摘Rockbursts are sudden and violent rock failures that can lead to huge production and equipment losses,injury or death of mining workers.Buckling has been regarded as one of the key mechanisms of rockbursts,which are often induced by dynamic loads from mining excavations,such as drilling and blasting in underground mining.The paper attempts to investigate the dynamic buckling mechanism of pillar rockbursts in underground mining,by considering rockbursts as a dynamic stability problem of underground rock structures.The results include:(1)A new explanation of the“sudden and violent”phenomenon of rockbursts,characterized by exponential growth of the amplitudes of transverse displacement responses,even in the presence of rock damping;(2)Identification of the critical role in inducing rockbursts of dynamic loads that bear frequencies approximately double the natural pillar frequency;(3)The greater influence on rockburst occurrence of the amplitude of dynamic component relative to the static component of loads;and(4)Quantification of the relative effects of stress waveform of dynamic loads on pillar rockbursts,which are in decreasing order if other parameters remain constant:rectangular,sinusoidal,and exponential waveforms.Application examples are provided and limitations of the approach are discussed.This research is motivated by the on-going and ubiquitous occurrence of rockbursts in underground excavations all around the world.In contrast to conventional methods that use rock specimens or rock materials to study rockbursts,this investigation emphasizes the structural effects on rockbursts,which has potential applications in hard rock mining engineering.
文摘Thelow cyclefatigue( LCF) propertiesof Ti 2 Al 2 5 Zralloytubeand rod specimens weredetermined at R.T. Thestress～strain curve equation under cyclic and monotonicloading andtherelation of totalstrain vsfatiguelife of Ti 2 Al 2 5Zr alloy were established. Theeffectsofstrain ratio R,strain amplitudeεaandspecimenshapeoncyclicdeformingbehavior and fatigueproperties were analyzed . The fatiguefracture features were also discussed ac cording as SEMobservation. The resultsshow , at R = 0 1 ,thecyclicstress straincurvesof Ti 2 Al 2 5Zr alloytube and rodspecimensappear ascyclesoftening. When Ris 1 ,itsrodspecimen behavesascyclesoftening;atlow εavalues; whereasitbehavesascyclehardeningat high εa values. The LCFproperties determined withtubespecimen islower apparentlythanthat withrod one. Thereare muchirregularrecrystallineconvexin LCFfracturesof Ti 2 Al 2 5Zr alloytubeand rodspecimens, and muchsecondarycracks,fatiguestriationsand holes alsoexistamongthem .
文摘Bajiaolian, one of the species in the Mayapple family ( Podophyllum pelatum ), has been widely used as a traditional Chinese herbal medicine for the remedies of snake bites, general weakness, poisons, condyloma accuminata, lymphadenopathy, and certain tumors in China. In Western medicine, Podophyllum was first used medically as a laxative in the early 19th century. Since 1940, the resin of podophyllum has also been used topically for various skin lesions, such as warts and condyloma. Human poisonings have been reported.An animal model was established to investigate the neurotoxic effects of Bajiaolian. Podophyllotoxin, the major active ingredient in Podophyllum, was injected (ip) to young adult male rats at doses of 0, 5, 10, or 15 mg-kg-1 b.w.. The animals were sacrificed 72 h after injection.Neuronal changes were readily observable in animals treated with 10 or 15 mg-kg-1 of the toxin. Edematous changes of the anterior horn motoneurons were observed in the spinal cord. No neuronal necrosis was found. The type of neuronal swelling is believed to be only a transient change and would probably subside with time if no further assaults occur. More serious and perhaps longer term of changes were found in the dorsal ganglion neurons and the nerve fibers (axons) in the central and peripheral nervous system. Severe depletion of the Nissl substance (RNA/polyribosomes) was observed in the dorsal root ganglion neurons. Alterations in these sensory neurons would give rise to and correlate with the sensory disturbances experienced by the patients. Bodian staining also revealed a dose-related increase in the coarseness (thickness) of the nerve fibers (axons) in the cerebellum, cerebral cortex, brainstem, and spinal cord.This is the first scientific study showing the neurotoxicity of Bajiaolian, a commonly used Chinese herbal medicine. Toxicities on other organ systems by this drug certainly exist. Caution should be exercised in the dispensing and usage of this medicine.
文摘Young male rats were orally intubated with podophyllotoxin: Group I, control animals, orally fed with vehicle only; Group Ⅱ, fed with an initial dose of 5 mg.kg-1 b.w., followed by a daily dose of 1.67 mg-kg-1 b.w. for 7d. Group III, fed with an initial dose of 15 mg.kg-1 b.w., followed by a daily dose of 5 mg.kg-1 b.w. for 7d. All animals were sacrificed 72 h after the last dosing.Histopathological examination revealed dose-related fatty change of the liver, atrophy andi degenerative changes of the intestinal epithelial linings and testicular seminiferous tubules. Depletion of the pancreatic acinar cell granules was also apparent in the Group III animals. No pathology, however, was observed in the kidneys. The present study demonstrated for the first time degenerative changes in the liver, intestine, testis, and pancreas of animals ingested podophyllotoxin. These pathological changes correlate well with the clinical signs/symptoms of abnormal liver function, abdominal pain and diarrhea, and reduced serum amylase in humans poisonded by podophyllum. Inhibition of protein synthesis and mitosis (disruption of microtubules) are believed to be the underlying mechanisms of these changes observed in the animals intoxicated by. podophyllotoxin.
