Whether in the monitoring of critically ill patients such as shock, respiratory failure, brain injury, or in major anesthesia surgeries, it is necessary to evaluate the patient’s pO<sub>2</sub> and pH. An...Whether in the monitoring of critically ill patients such as shock, respiratory failure, brain injury, or in major anesthesia surgeries, it is necessary to evaluate the patient’s pO<sub>2</sub> and pH. An optical fiber sensor presented is capable of monitoring the presence of oxygen partial pressure (pO<sub>2</sub>) and pH in the real-time. The sensor is based on fluorescence sensing of polymer immobilized in the oxygen/pH-sensitive membranes and covalently attached to the optical fiber probe. The design of this sensor uses LED as light source, which is an excitation light source, inducing specific wavelengths of fluorescence on the oxygen/pH-sensitive membrane. The intensity and lifetime of fluorescence are related to the pO<sub>2</sub> and pH. So the pO<sub>2</sub> and pH can be measured by the relationship between the pO<sub>2</sub>/pH values and the intensity and lifetime of fluorescence. The signal conditioning system based on DSP and STM32 was used to store and process data, and display test values. The response of the sensor for pO<sub>2</sub> and pH monitoring with nitrogen (N<sub>2</sub>) as a balancing gas in the laboratory was performed. Finally, the oxygen/pH sensing scheme presented in this work is intended for using in biological, medical and environmental applications.展开更多
文摘Whether in the monitoring of critically ill patients such as shock, respiratory failure, brain injury, or in major anesthesia surgeries, it is necessary to evaluate the patient’s pO<sub>2</sub> and pH. An optical fiber sensor presented is capable of monitoring the presence of oxygen partial pressure (pO<sub>2</sub>) and pH in the real-time. The sensor is based on fluorescence sensing of polymer immobilized in the oxygen/pH-sensitive membranes and covalently attached to the optical fiber probe. The design of this sensor uses LED as light source, which is an excitation light source, inducing specific wavelengths of fluorescence on the oxygen/pH-sensitive membrane. The intensity and lifetime of fluorescence are related to the pO<sub>2</sub> and pH. So the pO<sub>2</sub> and pH can be measured by the relationship between the pO<sub>2</sub>/pH values and the intensity and lifetime of fluorescence. The signal conditioning system based on DSP and STM32 was used to store and process data, and display test values. The response of the sensor for pO<sub>2</sub> and pH monitoring with nitrogen (N<sub>2</sub>) as a balancing gas in the laboratory was performed. Finally, the oxygen/pH sensing scheme presented in this work is intended for using in biological, medical and environmental applications.
基金Scientific Research Project of Shanxi Provincial Health Commission(Grant No.2021040)Postgraduate Education Innovation Plan of Shanxi Provincial Department of Education(Grant No.2022Y710)Shanxi University of Traditional Chinese Medicine Postgraduate Innovation and Entrepreneurship Project(Grant No.2022CX007)。
文摘本研究使用网络药理学、分子对接结合实验验证探究当归补血汤在糖尿病视网膜病变(Diabetic retinopathy,DR)中的作用机制。从TCMSP、TCMID、Swiss Target Prediction数据库筛选当归补血汤活性成分及其靶点,通过Gene Cards、Dis Ge NET收集DR靶点,利用Venny 2.1获得二者交集靶点。使用Cytoscape构建“疾病-药物-成分-靶点”、蛋白互作(PPI)网络;DAVID数据库对关键靶点进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)富集分析;Auto Dock Vina对主要活性成分与关键靶点进行分子对接验证。最后,通过动物实验验证网络药理学结果。收集到当归补血汤与DR共同靶点84个。GO分析显示220个条目;KEGG富集分析得到113条信号通路。分子对接结果显示关键靶点与槲皮素、豆甾醇等有效成分均具有较好的对接活性。动物实验表明当归补血汤可改善DR大鼠视网膜组织形态,降低血糖,增加胰岛素分泌,降低DR大鼠视网膜组织VEGF、TNF-α蛋白表达水平、提高PI3K、Akt1表达水平(P<0.05,P<0.01)。当归补血汤治疗DR具有多活性成分、多靶点、多通路的特点,可通过调节TNF-α、VEGFA、PI3K、AKT等核心靶点,影响PI3K/AKT、TNF等信号通路发挥对DR的治疗作用。
