AIM To investigate the hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells(h UC-MSCs) and to evaluate their therapeutic effect on liver fibrosis/cirrhosis.METHODS A CCl4-induced li...AIM To investigate the hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells(h UC-MSCs) and to evaluate their therapeutic effect on liver fibrosis/cirrhosis.METHODS A CCl4-induced liver fibrotic/cirrhotic rat model was used to assess the effect of h UC-MSCs. Histopathology was assessed by hematoxylin and eosin(H&E), Masson trichrome and Sirius red staining. The liver biochemical profile was measured using a Beckman Coulter analyzer. Expression analysis was performed using immunofluorescent staining, immunohistochemistry, Western blot, and real-time PCR.RESULTS We demonstrated that the infused h UC-MSCs could differentiate into hepatocytes in vivo. Functionally, the transplantation of h UC-MSCs to CCl4-treated rats improved liver transaminases and synthetic function, reduced liver histopathology and reversed hepatobiliary fibrosis. The reversal of hepatobiliary fibrosis was likely due to the reduced activation state of hepatic stellate cells, decreased collagen deposition, and enhanced extracellular matrix remodeling via the up-regulation of MMP-13 and down-regulation of TIMP-1. CONCLUSION Transplanted h UC-MSCs could differentiate into functional hepatocytes that improved both the biochemical and histopathologic changes in a CCl4-induced rat liver fibrosis model. h UC-MSCs may offer therapeutic opportunities for treating hepatobiliary diseases, including cirrhosis.展开更多
Background Recently,new anti-epileptic drugs （AEDs） have been more frequently selected to treat epilepsy.In the present study,we evaluated the dynamic changes of efficacy and safety of three newer AEDs for treating ...Background Recently,new anti-epileptic drugs （AEDs） have been more frequently selected to treat epilepsy.In the present study,we evaluated the dynamic changes of efficacy and safety of three newer AEDs for treating partial epilepsy in China.Methods Patients were collected sequentially and were divided into three groups which accepted oxcarbazepine （OXC）,lamotrigine （LTG） or topiramate （TPM） therapy.Each group included monotherapy and add-on therapy subgroups.We followed all patients for one year and recorded the indexes of efficacy and safety in detail.Results A total of 909 patients finished the follow-up observation.No significant difference was found in proportion of patients with 〉 or =50% reduction,〉 or =75% reduction and 100% seizure reduction in the LTG and OXC groups between the first and the second six months.In the TPM group there was a statistical difference between the first and the second six months in proportion of patients with 〉 or =50% reduction （P=-0.002）,〉 or =75% reduction （P 〈0.0001） and 100% seizure reduction （P=0.009） in the monotherapy subgroup,and about 〉 or =75% reduction and 100% seizure reduction in the add-on therapy subgroup （P 〈0.0001）.The efficacy between the add-on and monotherapy subgroups showed a statistical difference.The safety of the three newer AEDs was good.Conclusions The three newer AEDs all showed good efficacy and tolerability for partial epilepsy.And the efficacy can be maintained for at least one year.展开更多
This study aims to improve the mechanical behavior of disintegrated carbonaceous mudstone, which is used as road embankment filler in southwestern China. Triaxial tests were performed on disintegrated carbonaceous mud...This study aims to improve the mechanical behavior of disintegrated carbonaceous mudstone, which is used as road embankment filler in southwestern China. Triaxial tests were performed on disintegrated carbonaceous mudstone modified by fly ash, cement, and red clay. Then the stress-strain relationships and shear strength parameters were analyzed. The microstructure and mineral composition of the materials were identified via scanning electron microscopy and X-ray diffraction. The results show that the stress-strain relationships changed from strain-hardening to strain-softening when disintegrated carbonaceous mudstone was modified with cement. By contrast, the addition of fly ash and red clay did not change the type of stress-strain relationships. The order of these three additives is cement, red clay and fly ash according to their influences on the cohesion. Disintegrated carbonaceous mudstone without cement all showed bulging failures, and that modified with cement exhibited shear failures or bulging-shear failures. The soil particles of the improved soil were well bonded by cementitious substances, so the microstructure was denser and more stable, which highly enhanced the mechanical behavior of disintegrated carbonaceous mudstone. The findings could offer references for the use of carbonaceous mudstone in embankment engineering.展开更多
The Ti-20Zr-6.5Al-4V(T20Z,wt%)alloy surface was treated by the process of laser surface nitriding.The evolution of microstructures and microhardness has been investigated by changing the laser power parameter from 120...The Ti-20Zr-6.5Al-4V(T20Z,wt%)alloy surface was treated by the process of laser surface nitriding.The evolution of microstructures and microhardness has been investigated by changing the laser power parameter from 120 to 240 W.All laser-treated T20Z samples show two regions with distinctly different microstructural features,as compared with the untreated substrate:dense TiN dendrites and(α+β)-Ti(remelting zone,RMZ),nanoscaleαlaths doped with part of p phase(heat-affected zone,HAZ).The formation of TiN dendrites can be analyzed by a series of complex reactions during the process of melting and solidification.The increase in laser power results in the increase in content of TiN dendrite which is mainly due to the increase in energy input.In HAZ,the self-quenching effect leads to the formation of nanoscale a laths and the residue ofβphase.Microhardness profile of different regions was measured from the surface to the interior,and the highest microhardness was obtained(~HV 916.8)in the RMZ,as the laser power was set to 240 W.In the present study,we explained various microstructural characteristics induced by laser surface nitriding treatment.展开更多
Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf...Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.展开更多
Zinc Cr(Ⅲ)passivation layer(Zn-PL)could effectively protect NdFeB materials from corrosion.However,long-term exposure of Zn-PL to ambient atmosphere would reduce its wettability spontaneously,which hinders or deterio...Zinc Cr(Ⅲ)passivation layer(Zn-PL)could effectively protect NdFeB materials from corrosion.However,long-term exposure of Zn-PL to ambient atmosphere would reduce its wettability spontaneously,which hinders or deteriorates the adhesion of post-deposited materials.To solve this issue,a multilayer developed by layer-by-layer assembly approach was designed,and alumina sol and silica sol were prepared.A poly acrylic acid(PAA)layer was firstly deposited on Zn-PL to remove the preabsorbed organic carbon and form poly(zinc acrylate).Then,Al_(2)O_(3)and SiO_(2)sol-gel layers were sequentially assembled on PAA-coated Zn-PL.As a result,the hydrophobic Zn-PL turns into super-hydrophilic by coating organic-inorganic multilayers,which is attributed to the surface enrichment of hydrophilic hydroxyl groups and increases surface roughness.Furthermore,the super-hydrophilic surface displays excellent adhesion property without the negative effect on its other properties,which highlights a good prospect for a wide application of NdFeB or other potential materials.展开更多
BACKGROUND Follicular lymphoma(FL)is a type of B-cell lymphoma that originates at the germinal center and has a low malignancy rate.FL has become the most common inert lymphoma in Europe and America but has a relative...BACKGROUND Follicular lymphoma(FL)is a type of B-cell lymphoma that originates at the germinal center and has a low malignancy rate.FL has become the most common inert lymphoma in Europe and America but has a relatively low incidence in Asia.AIM To explore the clinical features,curative effects,and prognostic factors of FL.METHODS Completed medical records of 49 patients with FL who were admitted to the Ningbo First Hospital from June 2010 to June 2021 were examined.These patients were definitively diagnosed by pathological biopsy or immunohistochemical staining.The diagnostic criteria were based on the 2008 World Health Organization classification of lymphomas.Ann Arbor staging was performed according to the imaging and bone marrow examination results.Risk stratification of all patients was performed based on the International Prognostic Index(IPI),age-adjusted IPI,Follicular Lymphoma International Prognosis Index(FLIPI),and FLIPI2 to compare the efficacy of different treatment regimens and analyze the related prognostic factors.RESULTS The age of onset in patients ranged from 24 to 76 years,with a median age of 51 years.Most patients developed the disease at 40–59 years of age,and the male:female ratio was 1.6:1.No significant difference was noted in the curative effect between the non-chemotherapy,combined chemotherapy,and other chemotherapy regimens(P>0.05).Hemoglobin(Hb)level<120 g/L,Ki-67 value>50%,bone marrow involvement,and clinical stagesⅢ–IV were associated with a poor prognosis of FL(P<0.05).However,the influence of other indicators was not statistically significant.Risk grouping was performed using the FLIPI,and the results showed that 24.5%,40.8%,and 34.7%of patients were in the low-,moderate-,and high-risk groups,respectively.According to the survival analysis results,the survival rate of patients was lower in the high-risk group than in the other low-risk and moderate-risk groups(P<0.05).CONCLUSION FL mainly occurs in middle-aged and elderly men,primarily affecting lymph nodes and bone marrow展开更多
The East Asian monsoon has a tremendous impact on agricultural production in China. An assessment of the risk of drought disaster in maize-producing regions is therefore important in ensuring a reduction in such disas...The East Asian monsoon has a tremendous impact on agricultural production in China. An assessment of the risk of drought disaster in maize-producing regions is therefore important in ensuring a reduction in such disasters and an increase in food security. A risk assessment model, EPIC（Environmental Policy Integrated Climate） model, for maize drought disasters based on the Erosion Productivity Impact Calculator crop model is proposed for areas with the topographic characteristics of the mountainous karst region in southwest China. This region has one of the highest levels of environmental degradation in China. The results showed that the hazard risk level for the maize zone of southwest China is generally high. Most hazard index values were between 0.4 and 0.5,accounting for 47.32% of total study area. However,the risk level for drought loss was low. Most of the loss rate was ＆lt;0.1, accounting for 96.24% of the total study area. The three high-risk areas were mainlydistributed in the parallel ridge–valley areas in the east of Sichuan Province, the West Mountain area of Guizhou Province, and the south of Yunnan Province.These results provide a scientific basis and support for the reduction of agricultural drought disasters and an increase in food security in the southwest China maize zone.展开更多
文摘AIM To investigate the hepatic differentiation potential of human umbilical cord-derived mesenchymal stem cells(h UC-MSCs) and to evaluate their therapeutic effect on liver fibrosis/cirrhosis.METHODS A CCl4-induced liver fibrotic/cirrhotic rat model was used to assess the effect of h UC-MSCs. Histopathology was assessed by hematoxylin and eosin(H&E), Masson trichrome and Sirius red staining. The liver biochemical profile was measured using a Beckman Coulter analyzer. Expression analysis was performed using immunofluorescent staining, immunohistochemistry, Western blot, and real-time PCR.RESULTS We demonstrated that the infused h UC-MSCs could differentiate into hepatocytes in vivo. Functionally, the transplantation of h UC-MSCs to CCl4-treated rats improved liver transaminases and synthetic function, reduced liver histopathology and reversed hepatobiliary fibrosis. The reversal of hepatobiliary fibrosis was likely due to the reduced activation state of hepatic stellate cells, decreased collagen deposition, and enhanced extracellular matrix remodeling via the up-regulation of MMP-13 and down-regulation of TIMP-1. CONCLUSION Transplanted h UC-MSCs could differentiate into functional hepatocytes that improved both the biochemical and histopathologic changes in a CCl4-induced rat liver fibrosis model. h UC-MSCs may offer therapeutic opportunities for treating hepatobiliary diseases, including cirrhosis.
文摘Background Recently,new anti-epileptic drugs （AEDs） have been more frequently selected to treat epilepsy.In the present study,we evaluated the dynamic changes of efficacy and safety of three newer AEDs for treating partial epilepsy in China.Methods Patients were collected sequentially and were divided into three groups which accepted oxcarbazepine （OXC）,lamotrigine （LTG） or topiramate （TPM） therapy.Each group included monotherapy and add-on therapy subgroups.We followed all patients for one year and recorded the indexes of efficacy and safety in detail.Results A total of 909 patients finished the follow-up observation.No significant difference was found in proportion of patients with 〉 or =50% reduction,〉 or =75% reduction and 100% seizure reduction in the LTG and OXC groups between the first and the second six months.In the TPM group there was a statistical difference between the first and the second six months in proportion of patients with 〉 or =50% reduction （P=-0.002）,〉 or =75% reduction （P 〈0.0001） and 100% seizure reduction （P=0.009） in the monotherapy subgroup,and about 〉 or =75% reduction and 100% seizure reduction in the add-on therapy subgroup （P 〈0.0001）.The efficacy between the add-on and monotherapy subgroups showed a statistical difference.The safety of the three newer AEDs was good.Conclusions The three newer AEDs all showed good efficacy and tolerability for partial epilepsy.And the efficacy can be maintained for at least one year.
基金Projects(51908069, 51908073, 51838001, 51878070) supported by the National Natural Science Foundation of ChinaProject(2019SK2171) supported by the Key Research and Development Program of Hunan Province, China+3 种基金Project(2019IC04) supported by the Double First-Class Scientific Research International Cooperation Expansion Project of Changsha University of Science & Technology,ChinaProject(kfj190605) supported by the Open Fund of Engineering Laboratory of Spatial Information Technology of Highway Geological Disaster Early Warning in Hunan Province (Changsha University of Science & Technology), ChinaProject(kq1905043) supported by the Training Program for Excellent Young Innovators of Changsha, ChinaProject(SJCX202017) supported by the Practical Innovation Program for Graduates of Changsha University of Science & Technology, China。
文摘This study aims to improve the mechanical behavior of disintegrated carbonaceous mudstone, which is used as road embankment filler in southwestern China. Triaxial tests were performed on disintegrated carbonaceous mudstone modified by fly ash, cement, and red clay. Then the stress-strain relationships and shear strength parameters were analyzed. The microstructure and mineral composition of the materials were identified via scanning electron microscopy and X-ray diffraction. The results show that the stress-strain relationships changed from strain-hardening to strain-softening when disintegrated carbonaceous mudstone was modified with cement. By contrast, the addition of fly ash and red clay did not change the type of stress-strain relationships. The order of these three additives is cement, red clay and fly ash according to their influences on the cohesion. Disintegrated carbonaceous mudstone without cement all showed bulging failures, and that modified with cement exhibited shear failures or bulging-shear failures. The soil particles of the improved soil were well bonded by cementitious substances, so the microstructure was denser and more stable, which highly enhanced the mechanical behavior of disintegrated carbonaceous mudstone. The findings could offer references for the use of carbonaceous mudstone in embankment engineering.
基金financially supported by the Youth Top Talents Research Project of Hebei Provincial Education Department China(No.BJ2018052)the Natural Science Foundation of Hebei Province of China(Nos.E2019208205 and E2018208126)+3 种基金the National Natural Science Foundation of China(No.51701064)the Science and Technology on Plasma Dynamics Laboratory Fund Project(No.614220206021806)the Key Research and Development Program of Hebei Province(No.19211016D)the Open Foundation of State Key Laboratory of Metastable Materials Science and Technology(Nos.201804 and 201812).
文摘The Ti-20Zr-6.5Al-4V(T20Z,wt%)alloy surface was treated by the process of laser surface nitriding.The evolution of microstructures and microhardness has been investigated by changing the laser power parameter from 120 to 240 W.All laser-treated T20Z samples show two regions with distinctly different microstructural features,as compared with the untreated substrate:dense TiN dendrites and(α+β)-Ti(remelting zone,RMZ),nanoscaleαlaths doped with part of p phase(heat-affected zone,HAZ).The formation of TiN dendrites can be analyzed by a series of complex reactions during the process of melting and solidification.The increase in laser power results in the increase in content of TiN dendrite which is mainly due to the increase in energy input.In HAZ,the self-quenching effect leads to the formation of nanoscale a laths and the residue ofβphase.Microhardness profile of different regions was measured from the surface to the interior,and the highest microhardness was obtained(~HV 916.8)in the RMZ,as the laser power was set to 240 W.In the present study,we explained various microstructural characteristics induced by laser surface nitriding treatment.
基金supported by grants from the Key Project of Application Basic Research of Hebei Province,China (12965517D)the Youth Fund of Hebei Academy of Agriculture and Forestry Sciences,China (A09110103).
文摘Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.
基金financially supported by the National Natural Science Foundation of China(No.51371020)。
文摘Zinc Cr(Ⅲ)passivation layer(Zn-PL)could effectively protect NdFeB materials from corrosion.However,long-term exposure of Zn-PL to ambient atmosphere would reduce its wettability spontaneously,which hinders or deteriorates the adhesion of post-deposited materials.To solve this issue,a multilayer developed by layer-by-layer assembly approach was designed,and alumina sol and silica sol were prepared.A poly acrylic acid(PAA)layer was firstly deposited on Zn-PL to remove the preabsorbed organic carbon and form poly(zinc acrylate).Then,Al_(2)O_(3)and SiO_(2)sol-gel layers were sequentially assembled on PAA-coated Zn-PL.As a result,the hydrophobic Zn-PL turns into super-hydrophilic by coating organic-inorganic multilayers,which is attributed to the surface enrichment of hydrophilic hydroxyl groups and increases surface roughness.Furthermore,the super-hydrophilic surface displays excellent adhesion property without the negative effect on its other properties,which highlights a good prospect for a wide application of NdFeB or other potential materials.
基金Supported by Zhejiang TCM Science and Technology Project,No.2023ZL653。
文摘BACKGROUND Follicular lymphoma(FL)is a type of B-cell lymphoma that originates at the germinal center and has a low malignancy rate.FL has become the most common inert lymphoma in Europe and America but has a relatively low incidence in Asia.AIM To explore the clinical features,curative effects,and prognostic factors of FL.METHODS Completed medical records of 49 patients with FL who were admitted to the Ningbo First Hospital from June 2010 to June 2021 were examined.These patients were definitively diagnosed by pathological biopsy or immunohistochemical staining.The diagnostic criteria were based on the 2008 World Health Organization classification of lymphomas.Ann Arbor staging was performed according to the imaging and bone marrow examination results.Risk stratification of all patients was performed based on the International Prognostic Index(IPI),age-adjusted IPI,Follicular Lymphoma International Prognosis Index(FLIPI),and FLIPI2 to compare the efficacy of different treatment regimens and analyze the related prognostic factors.RESULTS The age of onset in patients ranged from 24 to 76 years,with a median age of 51 years.Most patients developed the disease at 40–59 years of age,and the male:female ratio was 1.6:1.No significant difference was noted in the curative effect between the non-chemotherapy,combined chemotherapy,and other chemotherapy regimens(P>0.05).Hemoglobin(Hb)level<120 g/L,Ki-67 value>50%,bone marrow involvement,and clinical stagesⅢ–IV were associated with a poor prognosis of FL(P<0.05).However,the influence of other indicators was not statistically significant.Risk grouping was performed using the FLIPI,and the results showed that 24.5%,40.8%,and 34.7%of patients were in the low-,moderate-,and high-risk groups,respectively.According to the survival analysis results,the survival rate of patients was lower in the high-risk group than in the other low-risk and moderate-risk groups(P<0.05).CONCLUSION FL mainly occurs in middle-aged and elderly men,primarily affecting lymph nodes and bone marrow
基金supported by National Natural Science Foundation of China (Grant Nos. 41301593 and 41471428)the Arid Meteorology Science Foundation, CMA (IAM201407)the State Key Development Program for BasicResearch of China (Grant No. 2012CB955402)
文摘The East Asian monsoon has a tremendous impact on agricultural production in China. An assessment of the risk of drought disaster in maize-producing regions is therefore important in ensuring a reduction in such disasters and an increase in food security. A risk assessment model, EPIC（Environmental Policy Integrated Climate） model, for maize drought disasters based on the Erosion Productivity Impact Calculator crop model is proposed for areas with the topographic characteristics of the mountainous karst region in southwest China. This region has one of the highest levels of environmental degradation in China. The results showed that the hazard risk level for the maize zone of southwest China is generally high. Most hazard index values were between 0.4 and 0.5,accounting for 47.32% of total study area. However,the risk level for drought loss was low. Most of the loss rate was ＆lt;0.1, accounting for 96.24% of the total study area. The three high-risk areas were mainlydistributed in the parallel ridge–valley areas in the east of Sichuan Province, the West Mountain area of Guizhou Province, and the south of Yunnan Province.These results provide a scientific basis and support for the reduction of agricultural drought disasters and an increase in food security in the southwest China maize zone.
