Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis,but the adverse effects of hyperuricemia on the liver have been seriously neglected.This research investigated the ameliorating effect...Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis,but the adverse effects of hyperuricemia on the liver have been seriously neglected.This research investigated the ameliorating effect of Lacticaseibacillus rhamnosus Fmb14 on hyperuricemia induced liver dysfunction both in vitro and in vivo.Cell free extracts of high dose L.rhamnosus Fmb14 treatment reduced the death rate of HepG2 cell lines from 24.1%to 14.9%by inhibiting NLRP3 recruitment,which was mainly activated by reactive oxygen species release and mitochondrial membrane potential disorder.In purine dietary induced hyperuricemia(PDIH)mice model,liver oedema and pyroptosis were ameliorated after L.rhamnosus Fmb14 administration through downregulating the expression levels of NLRP3,caspase-1 and gasdermin-D from 1.61 to 0.86,3.15 to 1.01 and 5.63 to 2.02,respectively.L.rhamnosus Fmb14 administration restored mitochondrial inner membrane protein(MPV17)and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice,indicating that dysbiosis of mitochondrial membrane potential was restored in liver.Intriguingly,PDIH pyroptosis stimulates the process of apoptosis,which leads to severe leakage of hepatocytes,and both of pyroptosis and apoptosis were decreased after L.rhamnosus Fmb14 treatment.Therefore,L.rhamnosus Fmb14 is a promising biological resource to maintain homeostasis of the liver in hyperuricemia and the prevention of subsequent complications.展开更多
Water-lubrication bearings are critical components in ship operation.However,studies on their maintenance and failure detection are highly limited.The use of sensors to continually monitor the working operation of bea...Water-lubrication bearings are critical components in ship operation.However,studies on their maintenance and failure detection are highly limited.The use of sensors to continually monitor the working operation of bearings is a potential approach to solve this problem,which is collectively called intelligent bearings.In this literature review,the recent progress of electrical resistance strain gauges,Fiber Bragg grating,triboelectric nanogenerators,piezoelectric nanogenerators,and thermoelectric sensors for in-situ monitoring is summarized.Future research and design concepts on intelligent water-lubrication bearings are also comprehensively discussed.The findings show that the accident risks,lubrication condition,and remaining life of water-lubricated bearings can be evaluated with the surface temperature,coefficient of friction,and wear volume monitoring.The research work on intelligent water-lubricated bearings is committed to promoting the development of green,electrified,and intelligent technologies for ship propulsion systems,which have important theoretical significance and application value.展开更多
基金Grant support was received from the National Natural Science Foundation of China(32072182).
文摘Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis,but the adverse effects of hyperuricemia on the liver have been seriously neglected.This research investigated the ameliorating effect of Lacticaseibacillus rhamnosus Fmb14 on hyperuricemia induced liver dysfunction both in vitro and in vivo.Cell free extracts of high dose L.rhamnosus Fmb14 treatment reduced the death rate of HepG2 cell lines from 24.1%to 14.9%by inhibiting NLRP3 recruitment,which was mainly activated by reactive oxygen species release and mitochondrial membrane potential disorder.In purine dietary induced hyperuricemia(PDIH)mice model,liver oedema and pyroptosis were ameliorated after L.rhamnosus Fmb14 administration through downregulating the expression levels of NLRP3,caspase-1 and gasdermin-D from 1.61 to 0.86,3.15 to 1.01 and 5.63 to 2.02,respectively.L.rhamnosus Fmb14 administration restored mitochondrial inner membrane protein(MPV17)and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice,indicating that dysbiosis of mitochondrial membrane potential was restored in liver.Intriguingly,PDIH pyroptosis stimulates the process of apoptosis,which leads to severe leakage of hepatocytes,and both of pyroptosis and apoptosis were decreased after L.rhamnosus Fmb14 treatment.Therefore,L.rhamnosus Fmb14 is a promising biological resource to maintain homeostasis of the liver in hyperuricemia and the prevention of subsequent complications.
基金Supported by the National Natural Science Foundation of China(Grant No.52171319).
文摘Water-lubrication bearings are critical components in ship operation.However,studies on their maintenance and failure detection are highly limited.The use of sensors to continually monitor the working operation of bearings is a potential approach to solve this problem,which is collectively called intelligent bearings.In this literature review,the recent progress of electrical resistance strain gauges,Fiber Bragg grating,triboelectric nanogenerators,piezoelectric nanogenerators,and thermoelectric sensors for in-situ monitoring is summarized.Future research and design concepts on intelligent water-lubrication bearings are also comprehensively discussed.The findings show that the accident risks,lubrication condition,and remaining life of water-lubricated bearings can be evaluated with the surface temperature,coefficient of friction,and wear volume monitoring.The research work on intelligent water-lubricated bearings is committed to promoting the development of green,electrified,and intelligent technologies for ship propulsion systems,which have important theoretical significance and application value.
