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基于质谱的多尺度代谢组学技术进展及其在生命医学研究中的应用 被引量:2
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作者 汪子怡 朱洪影 熊伟 《Science Bulletin》 SCIE EI CAS CSCD 2023年第19期2268-2284,M0004,共18页
代谢组学是20世纪后期出现的一个新兴研究领域,主要关注各类生命体中代谢物的全面分析.随着分析手段的飞速发展,这一领域在过去几十年取得了显著进步.精密的分析技术,如液相色谱-质谱(LCMS)、气相色谱-质谱(GC-MS)、毛细管电泳-质谱(CE-... 代谢组学是20世纪后期出现的一个新兴研究领域,主要关注各类生命体中代谢物的全面分析.随着分析手段的飞速发展,这一领域在过去几十年取得了显著进步.精密的分析技术,如液相色谱-质谱(LCMS)、气相色谱-质谱(GC-MS)、毛细管电泳-质谱(CE-MS)以及核磁共振等构成了代谢组分析的技术基础.基于这些技术手段的一系列全新改进和融合不断推动着代谢组学的发展.在近几十年里,单细胞或单细胞器水平上进行代谢组学检测一直是一个具有挑战性的任务,该领域近年来出现了重要突破,如单细胞质谱技术和单细胞器质谱技术实现了对细胞和细胞器水平上代谢组成分的检测,并深入揭示了代谢组在细胞或细胞器水平的异质性及其在生命活动中的重要意义;空间代谢组学使得原位代谢组分析成为可能,并结合三维重构技术清晰地展示了代谢物在组织内含量和分布情况.代谢组学技术的不断进步对于生物学和临床研究都具有重要意义,例如新型代谢通路的发现、细胞分化命运与代谢之间关系、以及病理诊断相关生物标志物等.本文总结了传统代谢组学技术和新型多尺度代谢组学技术的最新进展,以及这些技术在生物医学中的应用. 展开更多
关键词 代谢组学技术 质谱技术 代谢通路 细胞分化 病理诊断 生物标志物 核磁共振 毛细管电泳
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Effect of Cognitive Behavioral Intervention on Stress Response of Nurses with PTSD in Operating Room
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作者 Yi Huang Yajuan Ke +4 位作者 hongying zhu Yunsuo Gao Duozhi Wu Qiu Cai Juncheng Guo 《World Journal of Neuroscience》 2021年第1期8-16,共9页
<strong>Objective:</strong><span style="font-family:;" "=""> To investigate the effect of cognitive behavioral intervention on nurses with PTSD by using cognitive behavioral ... <strong>Objective:</strong><span style="font-family:;" "=""> To investigate the effect of cognitive behavioral intervention on nurses with PTSD by using cognitive behavioral intervention. <b>Methods:</b> The subjects were selected by randomized control method, and the PTSD nurses were divided into control group (n = 30) and intervention group (n = 30). The intervention group used cognitive behavioral intervention;the control group did not use cognitive behavioral intervention. Nurses with PTSD in the two groups com</span><span style="font-family:Verdana;"></span><span style="font-family:;" "="">pleted the basic situation survey, medical coping questionnaire (MCMQ) and anxiety self-rating form (SAS) psychological scale one month later. <b>Results:</b> There was no significant difference in MCMQ and SAS scores between the two groups before intervention (P > 0.05). After intervention, the scores of MCMQ and SAS in the intervention group were compared with the scores of MOCQ and SAS in the control group. The scores of the control group after intervention were significantly higher than those of the intervention group, with statistical significance (P < 0.05). There were statistically significant differences in MCMQ and SAS in the intervention group before and after intervention (P < 0.05). There was no statistical significance in the control group before and after intervention (P > 0.05). It shows that cognitive behavioral intervention therapy has the effect and value of improving the psychological status of nurses with PTSD. <b>Conclusion:</b> Cognitive behavioral intervention therapy combined with psychology for nurses with PTSD can effectively relieve their stress level, relieve their mood, and improve their positive coping ability and work efficiency.</span> 展开更多
关键词 Stress Response Cognitive Behavioral Therapy Operating Room Nurse Men-tal Health
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Hidden Dangers and Preventive Measures in the Inventory of Articles in Operating Room
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作者 Huang Yi Yajuan Ke +3 位作者 hongying zhu Suwen Lu Liurong Cheng Minghui Liu 《Open Journal of Preventive Medicine》 2021年第5期192-198,共7页
Operating Room is an important place for surgical diagnosis and treatment and rescue of patients. With the rapid development of medical technology, high, precision, sharp continuous development, operating room safety ... Operating Room is an important place for surgical diagnosis and treatment and rescue of patients. With the rapid development of medical technology, high, precision, sharp continuous development, operating room safety management is particularly important. The error of the counting of the surgical articles causes the foreign body left in the patient’s body, which will cause irreversible damage to the patient and even cause the medical dispute and touch the legal problem. Operation items inventory system is an important working system in the operating room. Scientific and standardized methods and systems for counting surgical instruments and dressings during operation can prevent foreign bodies from being left in the body, prevent errors and accidents in the operating room, and are very important for ensuring the safety of the operation of patients. In order to ensure the safety of patients, it is necessary to ensure the correct inventory of surgical items. The factors affecting the accuracy of item counting in the operating room and the preventive measures are summarized as follows. 展开更多
关键词 Operating Room ARTICLES Hidden Danger of Safety Preventive Measures Reference
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静音MR血管成像在颅内动脉瘤弹簧圈介入栓塞术后随访中的作用 被引量:18
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作者 尚松安 叶靖 +4 位作者 甄勇 罗先富 张洪英 朱庆强 吴晶涛 《中华放射学杂志》 CAS CSCD 北大核心 2016年第10期779-783,共5页
目的:探讨静音MRA在颅内动脉瘤弹簧圈介入栓塞术后随访中的应用价值。方法前瞻性收集行单纯弹簧圈介入栓塞术治疗的窄颈颅内动脉瘤患者15例,术后行DSA检查,并于DSA检查前当日行MRA检查。MRA图像采集分别采用静音MRA和时间飞跃法MRA(... 目的:探讨静音MRA在颅内动脉瘤弹簧圈介入栓塞术后随访中的应用价值。方法前瞻性收集行单纯弹簧圈介入栓塞术治疗的窄颈颅内动脉瘤患者15例,术后行DSA检查,并于DSA检查前当日行MRA检查。MRA图像采集分别采用静音MRA和时间飞跃法MRA(TOF MRA)。对栓塞动脉瘤周围血管结构采用4分法进行评估,并评价栓塞状态(分为完全栓塞或不完全栓塞)。采用Wilcoxon秩和检验比较静音MRA和TOF MRA间瘤周血管结构评分的差异。采用一致性(Kappa)检验评估静音MRA与TOF MRA间、静音MRA与DSA间、TOF MRA与DSA间所显示栓塞状态的一致性。结果 DSA显示完全栓塞11例,不完全栓塞4例。静音MRA和TOF MRA显示瘤周血管结构的评分分别为(3.50±0.62)、(3.00±0.63)分,差异有统计学意义(Z=-3.12,P=0.002)。15例静音MRA与DSA结果一致,具有高度一致性,Kappa值为0.82;13例TOF MRA与DSA结果一致,具有中度一致性, Kappa值为0.60;13例静音MRA与TOF MRA结果一致,具有高度一致性,Kappa值为0.76。静音MRA图像上显示残留瘤颈、瘤体清晰,TOF MRA图像上无法完整显示残留瘤颈。结论静音MRA显示颅内动脉瘤弹簧圈介入栓塞术后瘤周血管结构以及栓塞状态优于TOF MRA,与DSA结果一致。 展开更多
关键词 磁共振血管造影术 颅内动脉瘤 栓塞 治疗性 对比研究
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心肌细胞缺氧/复氧损伤下硫辛酸的保护性作用研究 被引量:1
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作者 逄晓玲 王雪原 +2 位作者 张洪颖 王倩 朱杰 《中国医师进修杂志》 2017年第9期821-824,共4页
目的 研究硫辛酸对H9c2心肌细胞缺氧/复氧损伤模型的保护作用,探讨其相关机制.方法 将8株H9c2大鼠心肌细胞株进行培养,待细胞生长至满视野时传代后分为常氧组、缺氧/复氧组和硫辛酸组.检测三组H9c2心肌细胞存活率、乳酸脱氢酶(LDH)活... 目的 研究硫辛酸对H9c2心肌细胞缺氧/复氧损伤模型的保护作用,探讨其相关机制.方法 将8株H9c2大鼠心肌细胞株进行培养,待细胞生长至满视野时传代后分为常氧组、缺氧/复氧组和硫辛酸组.检测三组H9c2心肌细胞存活率、乳酸脱氢酶(LDH)活性、丙二醛(MDA)和血红素氧合酶-1(HO-1),并进行比较.结果 缺氧/复氧组和硫辛酸组H9c2心肌细胞存活率明显低于常氧组[(52.86±6.39)%、(69.25±7.63)%比(92.31±7.82)%],但硫辛酸组明显高于缺氧/复氧组,差异均有统计学意义(P〈0.01).缺氧/复氧组和硫辛酸组LDH活性和MDA明显高于常氧组[(286.37±27.49)、(209.72±25.63)U/L比(126.32±18.94)U/L和(1.72±0.06)、(1.13±0.07)μmol/L比(0.68±0.06)μmol/L],但硫辛酸组明显低于缺氧/复氧组,差异均有统计学意义(P〈0.01).缺氧/复氧组和硫辛酸组HO-1明显高于常氧组[(213.71±18.94)%、(367.26±23.07)%比(87.92±19.23)%],而且硫辛酸组明显高于缺氧/复氧组,差异均有统计学意义(P〈0.01).结论 硫辛酸对心肌细胞缺氧/复氧损伤具有保护作用,增加HO-1含量,发挥其抗氧化应激的作用. 展开更多
关键词 再灌注损伤 硫辛酸 肌细胞 心脏 氧化性应激
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