Hepatocellular carcinoma （HCC） is currently the third leading cause of cancer mortality and a common poor-prognosis malignancy due to postoperative recurrence and metastasis. There is a significant correlation betwe...Hepatocellular carcinoma （HCC） is currently the third leading cause of cancer mortality and a common poor-prognosis malignancy due to postoperative recurrence and metastasis. There is a significant correlation between chronic hepatitis B virus （HBV） infection and hepatocarcinogenesis. As the first line of host defense against viral infections and tumors, natural killer （NK） cells express a large number of immune recognition receptors （NK receptors （NKRs）） to recognize ligands on hepatocytes, liver sinusoidal endothelial cells, stellate cells and Kupffer cells, which maintain the balance between immune response and immune tolerance of NK cells. Unfortunately, the percentage and absolute number of liver NK cells decrease significantly during the development and progression of HCC. The abnormal expression of NK cell receptors and dysfunction of liver NK cells contribute to the progression of chronic HBV infection and HCC and are significantly associated with poor prognosis for liver cancer. In this review, we focus on the role of NK cell receptors in anti-tumor immune responses in HCC, particularly HBV-related HCC. We discuss specifically how tumor cells evade attack from NK cells and how emerging understanding of NKRs may aid the development of novel treatments for HCC. Novel mono- and combination therapeutic strategies that target the NK cell receptor-ligand system may potentially lead to successful and effective immunotherapy in HCC.展开更多
Genomic data have demonstrated considerable traction in accelerating contemporary studies in traditional medicine. However,the lack of a uniform format and dispersed storage limits the full potential of herb genomic d...Genomic data have demonstrated considerable traction in accelerating contemporary studies in traditional medicine. However,the lack of a uniform format and dispersed storage limits the full potential of herb genomic data. In this study, we developed a Global Pharmacopoeia Genome Database(GPGD). The database contains 34,346 records for 903 herb species from eight global pharmacopoeias(Brazilian, Egyptian, European, Indian, Japanese, Korean, the Pharmacopoeia of the People’s Republic of China, and U.S. Pharmacopoeia’s Herbal Medicines Compendium). In particular, the GPGD contains 21,872 DNA barcodes from 867 species, 2,203 organelle genomes from 674 species, 55 whole genomes from 49 species, 534 genomic sequencing datasets from 366 species, and 9,682 transcriptome datasets from 350 species. Among the organelle genomes, 534 genomes from 366 species were newly generated in this study. Whole genomes, organelle genomes, genomic fragments, transcriptomes, and DNA barcodes were uniformly formatted and arranged by species. The GPGD is publicly accessible at http://www.gpgenome.com and serves as an essential resource for species identification, decomposition of biosynthetic pathways, and molecular-assisted breeding analysis. Thus, the database is an invaluable resource for future studies on herbal medicine safety, drug discovery, and the protection and rational use of herbal resources.展开更多
DBSCAN （density-based spatial clustering of ap- plications with noise） is an important spatial clustering tech- nique that is widely adopted in numerous applications. As the size of datasets is extremely large nowad...DBSCAN （density-based spatial clustering of ap- plications with noise） is an important spatial clustering tech- nique that is widely adopted in numerous applications. As the size of datasets is extremely large nowadays, parallel process- ing of complex data analysis such as DBSCAN becomes in- dispensable. However, there are three major drawbacks in the existing parallel DBSCAN algorithms. First, they fail to prop- erly balance the load among parallel tasks, especially when data are heavily skewed. Second, the scalability of these al- gorithms is limited because not all the critical sub-procedures are parallelized. Third, most of them are not primarily de- signed for shared-nothing environments, which makes them less portable to emerging parallel processing paradigms. In this paper, we present MR-DBSCAN, a scalable DBSCAN algorithm using MapReduce. In our algorithm, all the crit- ical sub-procedures are fully parallelized. As such, there is no performance bottleneck caused by sequential process- ing. Most importantly, we propose a novel data partitioning method based on computation cost estimation. The objective is to achieve desirable load balancing even in the context of heavily skewed data. Besides, We conduct our evaluation us- ing real large datasets with up to 1.2 billion points. The ex- periment results well confirm the efficiency and scalability of MR-DBSCAN.展开更多
Efficient immune responses against invading pathogens often involve coordination between cells from both the innate and adaptive immune systems.For multiple decades,it has been believed that CD8+memory T cells and nat...Efficient immune responses against invading pathogens often involve coordination between cells from both the innate and adaptive immune systems.For multiple decades,it has been believed that CD8+memory T cells and natural killer(NK)cells constantly and uniformly recirculate.Only recently was the existence of noncirculating memory T and NK cells that remain resident in the peripheral tissues,termed tissue-resident memory T(TRM)cells and tissue-resident NK(trNK)cells,observed in various organs owing to improved techniques.TRM cells populate a wide range of peripheral organs,including the skin,sensory ganglia,gut,lungs,brain,salivary glands,female reproductive tract,and others.Recent findings have demonstrated the existence of TRM in the secondary lymphoid organs(SLOs)as well,leading to revision of the classic theory that they exist only in peripheral organs.trNK cells have been identified in the uterus,skin,kidney,adipose tissue,and salivary glands.These tissue-resident lymphocytes do not recirculate in the blood or lymphatic system and often adopt a unique phenotype that is distinct from those of circulating immune cells.In this review,we will discuss the recent findings on the tissue residency of both innate and adaptive lymphocytes,with a particular focus on CD8+memory T cells,and describe some advances regarding unconventional T cells(invariant NKT cells,mucosal-associated invariant T cells(MAIT),andγδT cells)and the emerging family of trNK cells.Specifically,we will focus on the phenotypes and functions of these subsets and discuss their implications in anti-viral and anti-tumor immunity.展开更多
From the prehistoric era until the publishing of the Compendium of Materia Medica and the first scientific Nobel Prize in the Chinese mainland for Tu's discovery on anti-malarial tablets,each milestone and steppin...From the prehistoric era until the publishing of the Compendium of Materia Medica and the first scientific Nobel Prize in the Chinese mainland for Tu's discovery on anti-malarial tablets,each milestone and stepping stone in the developmental history of herbal medicine involved intrepid exploration,bold hypothesis formulation,and cautious verification.After thousands of years of discovery and development,herbal research has entered a new era—the era of herbgenomics.Herbgenomics combines herbal and genomic research,bridging the gap between traditional herbal medicine and cutting-edge omics studies.Therefore,it provides a general picture of the genetic background of traditional herbs,enabling researchers to investigate the mechanisms underlying the prevention and treatment of human diseases from an omics perspective.展开更多
Insulation performance of high voltage IGBT modules is one of the key attributes in power system applications.However,the existing standards of IGBT devices and research on the evaluation of insulation performance of ...Insulation performance of high voltage IGBT modules is one of the key attributes in power system applications.However,the existing standards of IGBT devices and research on the evaluation of insulation performance of high voltage IGBT modules are insufficient;for example,partial discharge resistance under DC voltage blocking condition is not considered.In this paper,a new test was proposed to allow the measurement of partial discharges in all the components of IGBT modules under DC voltage.The topology of the measuring circuit is arranged in the polarity discrimination way to exclude the interference,and the voltage and discharge current waveforms during the partial discharge process are measured by the wideband time domain measurement technique.According to the proposed test,the discharge phenomenon of the IGBT modules below the rating voltage were detected.A comprehensive waveform analysis on the voltage and discharge current was performed,and the influence of the applied voltage on the waveform parameters was obtained.The waveform parameters are influenced by the applied voltage and insulation structure,which enables the discrimination of the causes of the observed partial discharge in the IGBT module under DC voltage by the waveform analysis technique.Based on the waveform analysis technique,the types and causes of the observed partial discharges were discussed and inferred,and the correctness of the inference was further verified by observation.The proposed test and waveform analysis technique provide the possibility to evaluate and distinguish partial discharges in the high voltage IGBT module under DC voltage,which may be helpful to insulation performance evaluation and insulation defect diagnosis in high voltage IGBT module.展开更多
Organs such as the liver, uterus and lung possess hallmark immunotolerant features, making these organs important for sustaining self-homeostasis. These organs contain a relatively large amount of negative regulatory ...Organs such as the liver, uterus and lung possess hallmark immunotolerant features, making these organs important for sustaining self-homeostasis. These organs contain a relatively large amount of negative regulatory immune cells, which are believed to take part in the regulation of immune responses. Because natural killer cells constitute a large proportion of all lymphocytes in these organs, increasing attention has been given to the roles that these cells play in maintaining immunotolerance. Here, we review the distribution, differentiation, phenotypic features and functional features of natural killer cells in these immunotolerant organs, in addition to the influence of local microenvironments on these cells and how these factors contribute to organ-specific diseases.展开更多
The first research and experimental results obtained in China of high-accuracy radiometric calibration based on cryogenic radiometer are reported. Uncertainties of cryogenic radiometer and trap detectors at 7 waveleng...The first research and experimental results obtained in China of high-accuracy radiometric calibration based on cryogenic radiometer are reported. Uncertainties of cryogenic radiometer and trap detectors at 7 wavelengths in the visible spectrum (488-786 nm) were less than 0.023% and 0.035% respectively, which proved the reasonability and possibility of establishing and transferring high-accuracy radiometric standards based on detectors.展开更多
This paper presents a preliminary experimental investigation on magnetohydrodynamic （MHD） power generation using seeded supersonic argon flow as working fluid. Helium and argon are used as driver and driven gas resp...This paper presents a preliminary experimental investigation on magnetohydrodynamic （MHD） power generation using seeded supersonic argon flow as working fluid. Helium and argon are used as driver and driven gas respectively in a shock tunnel. Equilibrium contact surface operating mode is used to obtain high temperature gas, and the conductivity is obtained by adding seed K2CO3 powder into the driven section. Under the conditions of nozzle inlet total pressure being 0.32 MPa, total temperature 6 504 K, magnetic field density about 0.5 T and nozzle outlet velocity 1 959 m/s, induction voltage and short-circuit current of the segmentation MHD power generation channel are measured, and the experimental results agree with theoretical calculations; the average conductivity is about 20 S/m calculated from characteristics of voltage and current. When load factor is 0.5, the maximum power density of the MHD power generation channel reaches 4.797 1 MW/m3, and the maximum enthalpy extraction rate is 0.34%. Finally, the principle and method of indirect testing for gas state parameters are derived and analyzed.展开更多
The liver has been characterized as a frontline lymphoid organ with complex immunological features such as liver immunity and liver tolerance. Liver tolerance plays an important role in liver diseases including acute ...The liver has been characterized as a frontline lymphoid organ with complex immunological features such as liver immunity and liver tolerance. Liver tolerance plays an important role in liver diseases including acute inflammation, chronic infection, autoimmune disease, and tumors. The liver contains a large proportion of natural killer （NK） cells, which exhibit heterogeneity in phenotypic and functional characteristics. NK cell activation, well known for its role in the immune surveillance against tumor and pathogen-infected cells, depends on the balance between numerous activating and inhibitory signals. In addition to the innate direct ＂killer＂ functions, NK cell activity contributes to regulate innate and adaptive immunity （helper or regulator）. Under the setting of liver diseases, NK cells are of great importance for stimulating or inhibiting immune responses, leading to either immune activation or immune tolerance. Here, we focus on the relationship between NK cell biology, such as their phenotypic features and functional diversity, and liver diseases.展开更多
We build a high-resolution early aftershock catalog for the 2023 SE Türkiye seismic sequence with PALM,a seamless workflow that sequentially performs phase picking,association,location,and matched filter for cont...We build a high-resolution early aftershock catalog for the 2023 SE Türkiye seismic sequence with PALM,a seamless workflow that sequentially performs phase picking,association,location,and matched filter for continuous data.The catalog contains 29,519 well-located events in the two mainshocks rupture region during 2023-02-01–2023-02-28,which significantly improves the detection completeness and relocation precision compared to the public routine catalog.Employing the new PALM catalog,we analyze the structure of the seismogenic fault system.We find that the Eastern Anatolian Fault(EAF)that generated the first M_(W)7.9 mainshock is overall near-vertical,whereas complexities are revealed in a small-scale,such as subparallel subfaults,unmapped branches,and stepovers.The seismicity on EAF is shallow(<15 km)and concentrated in depth distribution,indicating a clear lock-creep transition.In contrast,the SürgüFault(SF)that is responsible for the second M_(W)7.8 mainshock is shovel-shaped for the nucleation segment and has overall low dip angles(~40°–80°).Aftershocks on the SF distribute in a broad range of depth,extending down to~35 km.We also analyze the temporal behavior of seismicity,discovering no immediate foreshocks within~5 days preceding the first mainshock,and no seismic activity on the SF before the second mainshock.展开更多
A hybrid finite-discrete element method was implemented to study the fracture process of rough rock joints under direct shearing. The hybrid method reproduced the joint shear resistance evolution process from asperity...A hybrid finite-discrete element method was implemented to study the fracture process of rough rock joints under direct shearing. The hybrid method reproduced the joint shear resistance evolution process from asperity sliding to degradation and from gouge formation to grinding. It is found that, in the direct shear test of rough rock joints under constant normal displacement loading conditions, higher shearing rate promotes the asperity degradation but constraints the volume dilation, which then results in higher peak shear resistance, more gouge formation and grinding, and smoother new joint surfaces. Moreover, it is found that the joint roughness affects the joint shear resistance evolution through influencing the joint fracture micro mechanism. The asperity degradation and gouge grinding are the main failure micro-mechanism in shearing rougher rock joints with deeper asperities while the asperity sliding is the main failure micro-mechanism in shearing smoother rock joints with shallower asperities. It is concluded that the hybrid finite-discrete element method is a valuable numerical tool better than traditional finite element method and discrete element method for modelling the joint sliding, asperity degradation, gouge formation, and gouge grinding occurred in the direct shear tests of rough rock joints.展开更多
Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and th...Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and there are currently no effective treatments. The development of neural stem cell(NSC) transplantation provides a promising strategy to treat neurodegenerative disease. However, the limited availability of NSCs prevents their application in neural disease therapy. In this study, we generated NSCs from induced pluripotent stem cells(iPSCs) and transplanted these cells into mice with experimental autoimmune encephalomyelitis(EAE), a model of MS. The results showed that transplantation of iPSC-derived NSCs dramatically reduced T cell infiltration and ameliorated white matter damage in the treated EAE mice. Correspondingly, the disease symptom score was greatly decreased, and motor ability was dramatically rescued in the iPSC-NSC-treated EAE mice, indicating the effectiveness of using iPSC-NSCs to treat MS. Our study provides pre-clinical evidence to support the feasibility of treating MS by transplantation of iPSC-derived NSCs.展开更多
Herbgenomics is an emerging field of traditional Chinese medicine(TCM)research and development.By combining TCM research with genomics,herbgenomics can help to establish the scientific validity of TCM and bring it int...Herbgenomics is an emerging field of traditional Chinese medicine(TCM)research and development.By combining TCM research with genomics,herbgenomics can help to establish the scientific validity of TCM and bring it into wider usage within the field of medicine.Salvia Linn.(S.Linn.)is a large genus of Labiatae that includes important medicinal plants.In this herbgenomics study,the complete chloroplast(cp)genomes of two Salvia spp.—namely,S.przewalskii and S.bulleyana,which are used as a surrogate for S.miltiorrhiza—were sequenced and compared with those of two other reported Salvia spp.—namely,S.miltiorrhiza and S.japonica.The genome organization,gene number,type,and repeat sequences were compared.The annotation results showed that both Salvia plants contain 114 unique genes,including 80 protein-coding,30 transfer RNA(tRNA),and four ribosomal RNA(rRNA)genes.Repeat sequence analysis revealed 21 forward and 22 palindromic sequences in both Salvia cp genomes,and 17 and 21 tandem repeats in S.przewalskii and S.bulleyana,respectively.A synteny comparison of the Salvia spp.cp genomes showed a high degree of sequence similarity in the coding regions and a relatively high divergence of the intergenic spacers.Pairwise alignment and single-nucleotide polymorphism(SNP)analyses found some candidate fragments to identify Salvia spp.,such as the intergenic region of the trnV–ndhC,trnQ–rps16,atpI–atpH,psbA–ycf3,ycf1,rpoC2,ndhF,matK,rpoB,rpoA,and accD genes.All of the results—including the repeat sequences and SNP sites,the inverted repeat(IR)region border,and the phylogenetic analysis—showed that S.przewalskii and S.bulleyana are extremely similar from a genetic standpoint.The cp genome sequences of the two Salvia spp.reported here will pave the way for breeding,species identification,phylogenetic evolution,and cp genetic engineering studies of Salvia medicinal plants.展开更多
Heavy metals have been viewed as hazardous environmental pollutants, and anthropogenic activities due to their high toxicity and persistent nature in the environment. Anthropogenic activities such as artisanal mining,...Heavy metals have been viewed as hazardous environmental pollutants, and anthropogenic activities due to their high toxicity and persistent nature in the environment. Anthropogenic activities such as artisanal mining, industrial activities, improper usage of fertilizers and pesticides, and indiscriminate open waste disposal bring about an increase in the presence of heavy metals in the environment. In the Keffi Metropolis, different elements lead to land contamination which debilitates soil quality, plant survival, human well-being, and the environment as a result of extensive dispersion or quantity of heavy metals in the soil and water. In recent years, biochar has emerged as a promising soil amendment for mitigating heavy metal pollution due to its unique physicochemical properties. This paper provides the effects of softwood pellet biochar on the retention of heavy metals in contaminated soils. A microcosm experiment was carried out to investigate the effects of biochar on the retention of heavy metals in contaminated soils. This research aimed to give an overview of the effects of softwood biochar at different temperatures (550˚C and 700˚C) on the retention of heavy metals and metalloids released from the soil during water inundation. The results show that the addition of organic matter (grass chippings) minimizes heavy metal mobilization. Also, biochar at high temperatures is more effective than those at low temperatures. The expected outcome of the research analysis includes providing insights into the role of biochar in retaining heavy metal contamination and further understanding the use of biochar as a sorbent for the management of contaminated soil.展开更多
Terahertz(THz)communication has been envisioned as a key enabling technology for sixthgeneration(6G).In this paper,we present an extensive THz channel measurement campaign for 6G wireless communications from 220 GHz t...Terahertz(THz)communication has been envisioned as a key enabling technology for sixthgeneration(6G).In this paper,we present an extensive THz channel measurement campaign for 6G wireless communications from 220 GHz to 330 GHz.Furthermore,the path loss is analyzed and modeled by using two single-frequency path loss models and a multiplefrequencies path loss model.It is found that at most frequency points,the measured path loss is larger than that in the free space.But at around 310 GHz,the propagation attenuation is relatively weaker compared to that in the free space.Also,the frequency dependence of path loss is observed and the frequency exponent of the multiple-frequencies path loss model is 2.1.Moreover,the cellular performance of THz communication systems is investigated by using the obtained path loss model.Simulation results indicate that the current inter-site distance(ISD)for the indoor scenario is too small for THz communications.Furthermore,the tremendous capacity gain can be obtained by using THz bands compared to using microwave bands and millimeter wave bands.Generally,this work can give an insight into the design and optimization of THz communication systems for 6G.展开更多
Gut microbiota and circulating metabolite dysbiosis predate important pathological changes in glucose metabolic disorders;however,comprehensive studies on impaired glucose tolerance(IGT),a diabetes mellitus(DM)precurs...Gut microbiota and circulating metabolite dysbiosis predate important pathological changes in glucose metabolic disorders;however,comprehensive studies on impaired glucose tolerance(IGT),a diabetes mellitus(DM)precursor,are lacking.Here,we perform metagenomic sequencing and metabolomics on 47 pairs of individuals with IGT and newly diagnosed DM and 46 controls with normal glucose tolerance(NGT);patients with IGT are followed up after 4 years for progression to DM.Analysis of baseline data reveals significant differences in gut microbiota and serum metabolites among the IGT,DM,and NGT groups.In addition,13 types of gut microbiota and 17 types of circulating metabolites showed significant differences at baseline before IGT progressed to DM,including higher levels of Eggerthella unclassified,Coprobacillus unclassified,Clostridium ramosum,L-valine,L-norleucine,and L-isoleucine,and lower levels of Eubacterium eligens,Bacteroides faecis,Lachnospiraceae bacterium 3_1_46FAA,Alistipes senegalensis,Megaspaera elsdenii,Clostridium perfringens,α-linolenic acid,10E,12Z-octadecadienoic acid,and dodecanoic acid.A random forest model based on differential intestinal microbiota and circulating metabolites can predict the progression from IGT to DM(AUC=0.87).These results suggest that microbiome and metabolome dysbiosis occur in individuals with IGT and have important predictive values and potential for intervention in preventing IGT from progressing to DM.展开更多
To further extend study on celestial attitude determination with strapdown star sensor from static into dynamic field, one prerequisite is to generate precise dynamic simulating star maps. First a neat analytical solu...To further extend study on celestial attitude determination with strapdown star sensor from static into dynamic field, one prerequisite is to generate precise dynamic simulating star maps. First a neat analytical solution of the smearing trajectory caused by spacecraft attitude maneuver is deduced successfully, whose parameters cover the geometric size of optics, three-axis angular velocities and CCD integral time. Then for the first time the mathematical law and method are discovered about how to synthesize the two formulae of smearing trajectory and the static Gaussian distribution function （GDF） model, the key of which is a line integral with regard to the static GDF attenuated by a factor 1/Ls （Ls is the arc length of the smearing trajectory） along the smearing trajectory. The dynamic smearing model is then obtained, also in an analytical form. After that, three sets of typical simulating maps and data are simulated from this dynamic model manifesting the expected smearing effects, also compatible with the linear model as its special case of no boresight rotation. Finally, model validity tests on a rate turntable are carried out, which results in a mean correlation coefficient 0.920 0 between the camera images and the corresponding model simulated ones with the same parameters. The sufficient similarity verifies the validity of the dynamic smearing model. This model, after pa- rameter calibration, can serve as a front-end loop of the ground semi-physical simulation system for celestial attitude determination with strapdown star sensor.展开更多
Two experiments were carried out on the same compact strip production (CSP) line, which differs in that one of them experienced γ→α→γ thermal history. The differences in microstructure, precipitation, misorientat...Two experiments were carried out on the same compact strip production (CSP) line, which differs in that one of them experienced γ→α→γ thermal history. The differences in microstructure, precipitation, misorientation etc between two experiments were investigated by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), electron back-scattered diffraction (EBSD) and positron annihilation technique (PAT). The carbon concentration in matrix is more inhomogeneous in the experiment than that with γ→α→γ7 thermal history. The specific precipitation characteristic in the experiment without γ→α→γ thermal history is discussed on the basis of different carbon diffusion behavior and interaction between dislocation and excess carbon.展开更多
基金This work was supported by grants from the Chinese Government Department of Science ＆ Technology of China （2012ZX10002006 2012ZX 10002014+4 种基金 2013 ZX 10002002 2012AA020901 2010CB911901 2013CB944901） and the Natural Science Foundation of China （81273220 81472646）.
文摘Hepatocellular carcinoma （HCC） is currently the third leading cause of cancer mortality and a common poor-prognosis malignancy due to postoperative recurrence and metastasis. There is a significant correlation between chronic hepatitis B virus （HBV） infection and hepatocarcinogenesis. As the first line of host defense against viral infections and tumors, natural killer （NK） cells express a large number of immune recognition receptors （NK receptors （NKRs）） to recognize ligands on hepatocytes, liver sinusoidal endothelial cells, stellate cells and Kupffer cells, which maintain the balance between immune response and immune tolerance of NK cells. Unfortunately, the percentage and absolute number of liver NK cells decrease significantly during the development and progression of HCC. The abnormal expression of NK cell receptors and dysfunction of liver NK cells contribute to the progression of chronic HBV infection and HCC and are significantly associated with poor prognosis for liver cancer. In this review, we focus on the role of NK cell receptors in anti-tumor immune responses in HCC, particularly HBV-related HCC. We discuss specifically how tumor cells evade attack from NK cells and how emerging understanding of NKRs may aid the development of novel treatments for HCC. Novel mono- and combination therapeutic strategies that target the NK cell receptor-ligand system may potentially lead to successful and effective immunotherapy in HCC.
基金supported by the National Key Research and Development Program of China (2019YFC1711100)the National Natural Science Foundation of China and Karst Science Research Center of Guizhou Province (U1812403-1)+4 种基金the Special Foundation for National Science and Technology Basic Research Program of China (2018FY100701)the Fundamental Research Funds for the Central Public Welfare Research Institutes (ZXKT17027, ZXKT18014)the Open Research Fund of Chengdu University of Traditional Chinese Medicine Key Laboratory of Systematic Research of Distinctive Chinese Medicine Resources in Southwest China (2020GZ2011016)the Funds for Fostering Outstanding Scholars in Science and Technology (Innovation) (ZZ13-YQ-047)Innovation Fund of China Academy of Chinese Medical Sciences
文摘Genomic data have demonstrated considerable traction in accelerating contemporary studies in traditional medicine. However,the lack of a uniform format and dispersed storage limits the full potential of herb genomic data. In this study, we developed a Global Pharmacopoeia Genome Database(GPGD). The database contains 34,346 records for 903 herb species from eight global pharmacopoeias(Brazilian, Egyptian, European, Indian, Japanese, Korean, the Pharmacopoeia of the People’s Republic of China, and U.S. Pharmacopoeia’s Herbal Medicines Compendium). In particular, the GPGD contains 21,872 DNA barcodes from 867 species, 2,203 organelle genomes from 674 species, 55 whole genomes from 49 species, 534 genomic sequencing datasets from 366 species, and 9,682 transcriptome datasets from 350 species. Among the organelle genomes, 534 genomes from 366 species were newly generated in this study. Whole genomes, organelle genomes, genomic fragments, transcriptomes, and DNA barcodes were uniformly formatted and arranged by species. The GPGD is publicly accessible at http://www.gpgenome.com and serves as an essential resource for species identification, decomposition of biosynthetic pathways, and molecular-assisted breeding analysis. Thus, the database is an invaluable resource for future studies on herbal medicine safety, drug discovery, and the protection and rational use of herbal resources.
文摘DBSCAN （density-based spatial clustering of ap- plications with noise） is an important spatial clustering tech- nique that is widely adopted in numerous applications. As the size of datasets is extremely large nowadays, parallel process- ing of complex data analysis such as DBSCAN becomes in- dispensable. However, there are three major drawbacks in the existing parallel DBSCAN algorithms. First, they fail to prop- erly balance the load among parallel tasks, especially when data are heavily skewed. Second, the scalability of these al- gorithms is limited because not all the critical sub-procedures are parallelized. Third, most of them are not primarily de- signed for shared-nothing environments, which makes them less portable to emerging parallel processing paradigms. In this paper, we present MR-DBSCAN, a scalable DBSCAN algorithm using MapReduce. In our algorithm, all the crit- ical sub-procedures are fully parallelized. As such, there is no performance bottleneck caused by sequential process- ing. Most importantly, we propose a novel data partitioning method based on computation cost estimation. The objective is to achieve desirable load balancing even in the context of heavily skewed data. Besides, We conduct our evaluation us- ing real large datasets with up to 1.2 billion points. The ex- periment results well confirm the efficiency and scalability of MR-DBSCAN.
基金by the National Key R&D Program of China(2018YFA0507403)the National Natural Science Foundation of China(81788101,81701631,31390433,and 31670908)the Chinese Academy of Sciences(XDB29030000).
文摘Efficient immune responses against invading pathogens often involve coordination between cells from both the innate and adaptive immune systems.For multiple decades,it has been believed that CD8+memory T cells and natural killer(NK)cells constantly and uniformly recirculate.Only recently was the existence of noncirculating memory T and NK cells that remain resident in the peripheral tissues,termed tissue-resident memory T(TRM)cells and tissue-resident NK(trNK)cells,observed in various organs owing to improved techniques.TRM cells populate a wide range of peripheral organs,including the skin,sensory ganglia,gut,lungs,brain,salivary glands,female reproductive tract,and others.Recent findings have demonstrated the existence of TRM in the secondary lymphoid organs(SLOs)as well,leading to revision of the classic theory that they exist only in peripheral organs.trNK cells have been identified in the uterus,skin,kidney,adipose tissue,and salivary glands.These tissue-resident lymphocytes do not recirculate in the blood or lymphatic system and often adopt a unique phenotype that is distinct from those of circulating immune cells.In this review,we will discuss the recent findings on the tissue residency of both innate and adaptive lymphocytes,with a particular focus on CD8+memory T cells,and describe some advances regarding unconventional T cells(invariant NKT cells,mucosal-associated invariant T cells(MAIT),andγδT cells)and the emerging family of trNK cells.Specifically,we will focus on the phenotypes and functions of these subsets and discuss their implications in anti-viral and anti-tumor immunity.
基金supported by the National Key Research and Development Program of China(2017-YFC1702100)the Fundamental Research Funds for the Central Public Welfare Research Institutes(L2017004)
文摘From the prehistoric era until the publishing of the Compendium of Materia Medica and the first scientific Nobel Prize in the Chinese mainland for Tu's discovery on anti-malarial tablets,each milestone and stepping stone in the developmental history of herbal medicine involved intrepid exploration,bold hypothesis formulation,and cautious verification.After thousands of years of discovery and development,herbal research has entered a new era—the era of herbgenomics.Herbgenomics combines herbal and genomic research,bridging the gap between traditional herbal medicine and cutting-edge omics studies.Therefore,it provides a general picture of the genetic background of traditional herbs,enabling researchers to investigate the mechanisms underlying the prevention and treatment of human diseases from an omics perspective.
基金This work was supported by the National Natural Science Foundation of China-State Grid Corporation Joint Fund for Smart Grid(No.U1766219)the Fundamental Research Funds for the Central Universities(No.2017XS027).
文摘Insulation performance of high voltage IGBT modules is one of the key attributes in power system applications.However,the existing standards of IGBT devices and research on the evaluation of insulation performance of high voltage IGBT modules are insufficient;for example,partial discharge resistance under DC voltage blocking condition is not considered.In this paper,a new test was proposed to allow the measurement of partial discharges in all the components of IGBT modules under DC voltage.The topology of the measuring circuit is arranged in the polarity discrimination way to exclude the interference,and the voltage and discharge current waveforms during the partial discharge process are measured by the wideband time domain measurement technique.According to the proposed test,the discharge phenomenon of the IGBT modules below the rating voltage were detected.A comprehensive waveform analysis on the voltage and discharge current was performed,and the influence of the applied voltage on the waveform parameters was obtained.The waveform parameters are influenced by the applied voltage and insulation structure,which enables the discrimination of the causes of the observed partial discharge in the IGBT module under DC voltage by the waveform analysis technique.Based on the waveform analysis technique,the types and causes of the observed partial discharges were discussed and inferred,and the correctness of the inference was further verified by observation.The proposed test and waveform analysis technique provide the possibility to evaluate and distinguish partial discharges in the high voltage IGBT module under DC voltage,which may be helpful to insulation performance evaluation and insulation defect diagnosis in high voltage IGBT module.
文摘Organs such as the liver, uterus and lung possess hallmark immunotolerant features, making these organs important for sustaining self-homeostasis. These organs contain a relatively large amount of negative regulatory immune cells, which are believed to take part in the regulation of immune responses. Because natural killer cells constitute a large proportion of all lymphocytes in these organs, increasing attention has been given to the roles that these cells play in maintaining immunotolerance. Here, we review the distribution, differentiation, phenotypic features and functional features of natural killer cells in these immunotolerant organs, in addition to the influence of local microenvironments on these cells and how these factors contribute to organ-specific diseases.
文摘The first research and experimental results obtained in China of high-accuracy radiometric calibration based on cryogenic radiometer are reported. Uncertainties of cryogenic radiometer and trap detectors at 7 wavelengths in the visible spectrum (488-786 nm) were less than 0.023% and 0.035% respectively, which proved the reasonability and possibility of establishing and transferring high-accuracy radiometric standards based on detectors.
基金National Natural Science Foundation of China （10972236） The Scientific and Technical Innovation for Postgraduates of Air Force Engineering University （DX2010102）
文摘This paper presents a preliminary experimental investigation on magnetohydrodynamic （MHD） power generation using seeded supersonic argon flow as working fluid. Helium and argon are used as driver and driven gas respectively in a shock tunnel. Equilibrium contact surface operating mode is used to obtain high temperature gas, and the conductivity is obtained by adding seed K2CO3 powder into the driven section. Under the conditions of nozzle inlet total pressure being 0.32 MPa, total temperature 6 504 K, magnetic field density about 0.5 T and nozzle outlet velocity 1 959 m/s, induction voltage and short-circuit current of the segmentation MHD power generation channel are measured, and the experimental results agree with theoretical calculations; the average conductivity is about 20 S/m calculated from characteristics of voltage and current. When load factor is 0.5, the maximum power density of the MHD power generation channel reaches 4.797 1 MW/m3, and the maximum enthalpy extraction rate is 0.34%. Finally, the principle and method of indirect testing for gas state parameters are derived and analyzed.
基金This work was supported by grants from the National Natural Science Foundation of China （Nos. 81771685, 91429303, 31390433, 81761128013, and 91542000） and Ministry of Science and Technology of China （973 Basic Science Project, No. 2013CB944902）.
文摘The liver has been characterized as a frontline lymphoid organ with complex immunological features such as liver immunity and liver tolerance. Liver tolerance plays an important role in liver diseases including acute inflammation, chronic infection, autoimmune disease, and tumors. The liver contains a large proportion of natural killer （NK） cells, which exhibit heterogeneity in phenotypic and functional characteristics. NK cell activation, well known for its role in the immune surveillance against tumor and pathogen-infected cells, depends on the balance between numerous activating and inhibitory signals. In addition to the innate direct ＂killer＂ functions, NK cell activity contributes to regulate innate and adaptive immunity （helper or regulator）. Under the setting of liver diseases, NK cells are of great importance for stimulating or inhibiting immune responses, leading to either immune activation or immune tolerance. Here, we focus on the relationship between NK cell biology, such as their phenotypic features and functional diversity, and liver diseases.
基金jointly supported by the National Key R&D Program (No.2022YFF0800601)the Istanbul Technical University Research Fund (ITU-BAP)+1 种基金the Alexander von Humboldt Foundation Research Fellowship Award for providing computing facilities through the Humboldt-Stiftung Follow-Up Programthe University of California,Riverside。
文摘We build a high-resolution early aftershock catalog for the 2023 SE Türkiye seismic sequence with PALM,a seamless workflow that sequentially performs phase picking,association,location,and matched filter for continuous data.The catalog contains 29,519 well-located events in the two mainshocks rupture region during 2023-02-01–2023-02-28,which significantly improves the detection completeness and relocation precision compared to the public routine catalog.Employing the new PALM catalog,we analyze the structure of the seismogenic fault system.We find that the Eastern Anatolian Fault(EAF)that generated the first M_(W)7.9 mainshock is overall near-vertical,whereas complexities are revealed in a small-scale,such as subparallel subfaults,unmapped branches,and stepovers.The seismicity on EAF is shallow(<15 km)and concentrated in depth distribution,indicating a clear lock-creep transition.In contrast,the SürgüFault(SF)that is responsible for the second M_(W)7.8 mainshock is shovel-shaped for the nucleation segment and has overall low dip angles(~40°–80°).Aftershocks on the SF distribute in a broad range of depth,extending down to~35 km.We also analyze the temporal behavior of seismicity,discovering no immediate foreshocks within~5 days preceding the first mainshock,and no seismic activity on the SF before the second mainshock.
基金The first author would like to thank the supports of the NARGS, IRGS and AAS grants of Australia, and the National Science Foundation grants （No. 51574060 and No. 51079017） of China, in which the first author is the intemational collaborator. The academic visits of the third and fourth authors to the University of Tasmania are partly supported by a PhD visiting scholarship and an academic visiting scholarship, respectively, provided by the China Scholarship Council, which are greatly appreciated.
文摘A hybrid finite-discrete element method was implemented to study the fracture process of rough rock joints under direct shearing. The hybrid method reproduced the joint shear resistance evolution process from asperity sliding to degradation and from gouge formation to grinding. It is found that, in the direct shear test of rough rock joints under constant normal displacement loading conditions, higher shearing rate promotes the asperity degradation but constraints the volume dilation, which then results in higher peak shear resistance, more gouge formation and grinding, and smoother new joint surfaces. Moreover, it is found that the joint roughness affects the joint shear resistance evolution through influencing the joint fracture micro mechanism. The asperity degradation and gouge grinding are the main failure micro-mechanism in shearing rougher rock joints with deeper asperities while the asperity sliding is the main failure micro-mechanism in shearing smoother rock joints with shallower asperities. It is concluded that the hybrid finite-discrete element method is a valuable numerical tool better than traditional finite element method and discrete element method for modelling the joint sliding, asperity degradation, gouge formation, and gouge grinding occurred in the direct shear tests of rough rock joints.
基金supported by the China National Basic Research Program(2013CB966901,2012CBA01303)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA01040108)+1 种基金National Thousand Young Talents Program to Tongbiao Zhaothe National Natural Science Foundation of China Program((31271592,31570995)to Tongbiao Zhao,(31400831)to Jiani Cao)
文摘Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and there are currently no effective treatments. The development of neural stem cell(NSC) transplantation provides a promising strategy to treat neurodegenerative disease. However, the limited availability of NSCs prevents their application in neural disease therapy. In this study, we generated NSCs from induced pluripotent stem cells(iPSCs) and transplanted these cells into mice with experimental autoimmune encephalomyelitis(EAE), a model of MS. The results showed that transplantation of iPSC-derived NSCs dramatically reduced T cell infiltration and ameliorated white matter damage in the treated EAE mice. Correspondingly, the disease symptom score was greatly decreased, and motor ability was dramatically rescued in the iPSC-NSC-treated EAE mice, indicating the effectiveness of using iPSC-NSCs to treat MS. Our study provides pre-clinical evidence to support the feasibility of treating MS by transplantation of iPSC-derived NSCs.
基金This work is supported by the National Nature Science Foundation of China(QFSL2018004,2017YFC1702100,and 81741060)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZXKT17004).
文摘Herbgenomics is an emerging field of traditional Chinese medicine(TCM)research and development.By combining TCM research with genomics,herbgenomics can help to establish the scientific validity of TCM and bring it into wider usage within the field of medicine.Salvia Linn.(S.Linn.)is a large genus of Labiatae that includes important medicinal plants.In this herbgenomics study,the complete chloroplast(cp)genomes of two Salvia spp.—namely,S.przewalskii and S.bulleyana,which are used as a surrogate for S.miltiorrhiza—were sequenced and compared with those of two other reported Salvia spp.—namely,S.miltiorrhiza and S.japonica.The genome organization,gene number,type,and repeat sequences were compared.The annotation results showed that both Salvia plants contain 114 unique genes,including 80 protein-coding,30 transfer RNA(tRNA),and four ribosomal RNA(rRNA)genes.Repeat sequence analysis revealed 21 forward and 22 palindromic sequences in both Salvia cp genomes,and 17 and 21 tandem repeats in S.przewalskii and S.bulleyana,respectively.A synteny comparison of the Salvia spp.cp genomes showed a high degree of sequence similarity in the coding regions and a relatively high divergence of the intergenic spacers.Pairwise alignment and single-nucleotide polymorphism(SNP)analyses found some candidate fragments to identify Salvia spp.,such as the intergenic region of the trnV–ndhC,trnQ–rps16,atpI–atpH,psbA–ycf3,ycf1,rpoC2,ndhF,matK,rpoB,rpoA,and accD genes.All of the results—including the repeat sequences and SNP sites,the inverted repeat(IR)region border,and the phylogenetic analysis—showed that S.przewalskii and S.bulleyana are extremely similar from a genetic standpoint.The cp genome sequences of the two Salvia spp.reported here will pave the way for breeding,species identification,phylogenetic evolution,and cp genetic engineering studies of Salvia medicinal plants.
文摘Heavy metals have been viewed as hazardous environmental pollutants, and anthropogenic activities due to their high toxicity and persistent nature in the environment. Anthropogenic activities such as artisanal mining, industrial activities, improper usage of fertilizers and pesticides, and indiscriminate open waste disposal bring about an increase in the presence of heavy metals in the environment. In the Keffi Metropolis, different elements lead to land contamination which debilitates soil quality, plant survival, human well-being, and the environment as a result of extensive dispersion or quantity of heavy metals in the soil and water. In recent years, biochar has emerged as a promising soil amendment for mitigating heavy metal pollution due to its unique physicochemical properties. This paper provides the effects of softwood pellet biochar on the retention of heavy metals in contaminated soils. A microcosm experiment was carried out to investigate the effects of biochar on the retention of heavy metals in contaminated soils. This research aimed to give an overview of the effects of softwood biochar at different temperatures (550˚C and 700˚C) on the retention of heavy metals and metalloids released from the soil during water inundation. The results show that the addition of organic matter (grass chippings) minimizes heavy metal mobilization. Also, biochar at high temperatures is more effective than those at low temperatures. The expected outcome of the research analysis includes providing insights into the role of biochar in retaining heavy metal contamination and further understanding the use of biochar as a sorbent for the management of contaminated soil.
基金supported by the National Science Fund for Distinguished Young Scholars(No.61925102)the National Key R&D Program of China(No.2020YFB1805002)the Key Project of State Key Lab of Networking and Switching Technology(No.NST20180105).
文摘Terahertz(THz)communication has been envisioned as a key enabling technology for sixthgeneration(6G).In this paper,we present an extensive THz channel measurement campaign for 6G wireless communications from 220 GHz to 330 GHz.Furthermore,the path loss is analyzed and modeled by using two single-frequency path loss models and a multiplefrequencies path loss model.It is found that at most frequency points,the measured path loss is larger than that in the free space.But at around 310 GHz,the propagation attenuation is relatively weaker compared to that in the free space.Also,the frequency dependence of path loss is observed and the frequency exponent of the multiple-frequencies path loss model is 2.1.Moreover,the cellular performance of THz communication systems is investigated by using the obtained path loss model.Simulation results indicate that the current inter-site distance(ISD)for the indoor scenario is too small for THz communications.Furthermore,the tremendous capacity gain can be obtained by using THz bands compared to using microwave bands and millimeter wave bands.Generally,this work can give an insight into the design and optimization of THz communication systems for 6G.
基金supported by the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202001)the National Natural Science Foundation of China(82104835).
文摘Gut microbiota and circulating metabolite dysbiosis predate important pathological changes in glucose metabolic disorders;however,comprehensive studies on impaired glucose tolerance(IGT),a diabetes mellitus(DM)precursor,are lacking.Here,we perform metagenomic sequencing and metabolomics on 47 pairs of individuals with IGT and newly diagnosed DM and 46 controls with normal glucose tolerance(NGT);patients with IGT are followed up after 4 years for progression to DM.Analysis of baseline data reveals significant differences in gut microbiota and serum metabolites among the IGT,DM,and NGT groups.In addition,13 types of gut microbiota and 17 types of circulating metabolites showed significant differences at baseline before IGT progressed to DM,including higher levels of Eggerthella unclassified,Coprobacillus unclassified,Clostridium ramosum,L-valine,L-norleucine,and L-isoleucine,and lower levels of Eubacterium eligens,Bacteroides faecis,Lachnospiraceae bacterium 3_1_46FAA,Alistipes senegalensis,Megaspaera elsdenii,Clostridium perfringens,α-linolenic acid,10E,12Z-octadecadienoic acid,and dodecanoic acid.A random forest model based on differential intestinal microbiota and circulating metabolites can predict the progression from IGT to DM(AUC=0.87).These results suggest that microbiome and metabolome dysbiosis occur in individuals with IGT and have important predictive values and potential for intervention in preventing IGT from progressing to DM.
文摘To further extend study on celestial attitude determination with strapdown star sensor from static into dynamic field, one prerequisite is to generate precise dynamic simulating star maps. First a neat analytical solution of the smearing trajectory caused by spacecraft attitude maneuver is deduced successfully, whose parameters cover the geometric size of optics, three-axis angular velocities and CCD integral time. Then for the first time the mathematical law and method are discovered about how to synthesize the two formulae of smearing trajectory and the static Gaussian distribution function （GDF） model, the key of which is a line integral with regard to the static GDF attenuated by a factor 1/Ls （Ls is the arc length of the smearing trajectory） along the smearing trajectory. The dynamic smearing model is then obtained, also in an analytical form. After that, three sets of typical simulating maps and data are simulated from this dynamic model manifesting the expected smearing effects, also compatible with the linear model as its special case of no boresight rotation. Finally, model validity tests on a rate turntable are carried out, which results in a mean correlation coefficient 0.920 0 between the camera images and the corresponding model simulated ones with the same parameters. The sufficient similarity verifies the validity of the dynamic smearing model. This model, after pa- rameter calibration, can serve as a front-end loop of the ground semi-physical simulation system for celestial attitude determination with strapdown star sensor.
基金Authors acknowledge the financial support from the National Natural Science Foundation of China(No.50334010).
文摘Two experiments were carried out on the same compact strip production (CSP) line, which differs in that one of them experienced γ→α→γ thermal history. The differences in microstructure, precipitation, misorientation etc between two experiments were investigated by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), electron back-scattered diffraction (EBSD) and positron annihilation technique (PAT). The carbon concentration in matrix is more inhomogeneous in the experiment than that with γ→α→γ7 thermal history. The specific precipitation characteristic in the experiment without γ→α→γ thermal history is discussed on the basis of different carbon diffusion behavior and interaction between dislocation and excess carbon.
