Internode number and length are the foundation to constitute plant height, ear height and the above-ground spatial structure of maize plant. In this study, segregating populations were constructed between EHel with ex...Internode number and length are the foundation to constitute plant height, ear height and the above-ground spatial structure of maize plant. In this study, segregating populations were constructed between EHel with extremely low ear height and B73. Through the SNP-based genotyping and phenotypic characterization, 13 QTL distributed on the chromosomes (Chrs) of Chr1, Chr2, Chr5-Chr8 were detected for four traits of internode no. above ear (INa), average internode length above ear (ILaa), internode no. below ear (INb), and average internode length below ear (ILab). Phenotypic variation explained (PVE) by a single QTL ranged from 6.82% (qILab2-2) to 12.99% (qILaa5). Zm00001d016823 within the physical region of qILaa5, the major QTL for ILaa with the largest PVE was determined as the candidate through the genomic annotation and sequence alignment between EHel and B73. Product of Zm00001d016823 was annotated as a WEB family protein homogenous to At1g75720. qRT-PCR assay showed that Zm00001d016823 highly expressed within the tissue of internode, exhibiting statistically higher expression levels among internodes of IN4 to IN7 in EHel than those in B73 (P Zm00001d016823 might provide novel insight into molecular mechanism beyond phytohormones controlling internode development in maize.展开更多
Nitrogen (N) plays a vital role in the productivity of maize (<i>Zea</i> <i>mays</i> L). To investigate the fertilizer effects of N on the yield and growth of maize hybrid variety (Gorilla), th...Nitrogen (N) plays a vital role in the productivity of maize (<i>Zea</i> <i>mays</i> L). To investigate the fertilizer effects of N on the yield and growth of maize hybrid variety (Gorilla), the experiment was carried out at the research farm of University of Swabi, Pakistan, during summer 2017-18. Four levels of N (Urea, Urea + Farm Yard Manure (FYM), Urea + Compost, Urea + Poultry Manure (PM)) were set in the present study. Randomized complete block design (RCBD) was used with split-plot arrangement with N administering to main plot. Results showed that yield and other traits, <i>i.e.</i> plant height, ear length, ear weight, kernel yield, kernels ear<sup>-1</sup> and harvest index (HI) were significantly affected by Nitrogen. In current study, the maximum performances of plant height (231.46 cm), ear length (12.17 cm), kernels ear<sup>-1</sup> (434.83), kernel yield (2095.7 kg·ha<sup>-1</sup>), total kernel weight (350.75 kg·ha<sup>-1</sup>), biomass yield (4015.3 kg·ha<sup>-1</sup>) and HI (37.31) were recorded under the treatment of UREA + PM, and followed by UREA + FYM. Besides, the applications of organic manure in combination with nitrogen significantly increased yield and its components. Application of 50% of N and 50% of poultry manure produced higher performance for the traits of plant height, ear length, kernels ear<sup>-1</sup>, total kernels weight ear<sup>-1</sup>, kernel yield, and biomass yield.展开更多
Wild relatives possess potential genetic diversity for maize (<i><span style="font-family:Verdana;">Zea mays</span></i><span style="font-family:Verdana;"> L.) improvem...Wild relatives possess potential genetic diversity for maize (<i><span style="font-family:Verdana;">Zea mays</span></i><span style="font-family:Verdana;"> L.) improvement. Characterization of maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> introgression lines (ILs) is of great value to diversify the genetic base and improve the maize germplasm. Four maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> IL generations, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;"> BC1, BC2, BC3, and RIL, were constructed under the elite inbred background of 48-2, elite inbred line that is widely used in maize breeding in Southwestern China, and were phenotyped in different years and genotyped with 56110 SNPs. The results indicated that 48-2 had higher phenotypic performances than all the characterized ILs on most of the agronomic traits. Compared with other ILs, BC2 individuals exhibited more similar performance to 48-2 on most traits and possessed the highest kernel ratio (66.5%). Population structure and principal component analysis indicated that BC3 individuals gathered closer to 48-2 and exhibited the lowest </span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;">-introgression frequency (0.50%), while BC2 (29.06%) and RIL (18.52%) showed higher introgression frequency. The high level of genetic diversity observed in the maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> ILs demonstrated that </span><i><span style="font-family:Verdana;">Z</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">mays</span></i><span style="font-family:Verdana;"> ssp. </span><i><span style="font-family:Verdana;">mexicana </span></i><span style="font-family:Verdana;">can serve as a potential source for the enrichment of maize germplasm.</span>展开更多
文摘Internode number and length are the foundation to constitute plant height, ear height and the above-ground spatial structure of maize plant. In this study, segregating populations were constructed between EHel with extremely low ear height and B73. Through the SNP-based genotyping and phenotypic characterization, 13 QTL distributed on the chromosomes (Chrs) of Chr1, Chr2, Chr5-Chr8 were detected for four traits of internode no. above ear (INa), average internode length above ear (ILaa), internode no. below ear (INb), and average internode length below ear (ILab). Phenotypic variation explained (PVE) by a single QTL ranged from 6.82% (qILab2-2) to 12.99% (qILaa5). Zm00001d016823 within the physical region of qILaa5, the major QTL for ILaa with the largest PVE was determined as the candidate through the genomic annotation and sequence alignment between EHel and B73. Product of Zm00001d016823 was annotated as a WEB family protein homogenous to At1g75720. qRT-PCR assay showed that Zm00001d016823 highly expressed within the tissue of internode, exhibiting statistically higher expression levels among internodes of IN4 to IN7 in EHel than those in B73 (P Zm00001d016823 might provide novel insight into molecular mechanism beyond phytohormones controlling internode development in maize.
文摘Nitrogen (N) plays a vital role in the productivity of maize (<i>Zea</i> <i>mays</i> L). To investigate the fertilizer effects of N on the yield and growth of maize hybrid variety (Gorilla), the experiment was carried out at the research farm of University of Swabi, Pakistan, during summer 2017-18. Four levels of N (Urea, Urea + Farm Yard Manure (FYM), Urea + Compost, Urea + Poultry Manure (PM)) were set in the present study. Randomized complete block design (RCBD) was used with split-plot arrangement with N administering to main plot. Results showed that yield and other traits, <i>i.e.</i> plant height, ear length, ear weight, kernel yield, kernels ear<sup>-1</sup> and harvest index (HI) were significantly affected by Nitrogen. In current study, the maximum performances of plant height (231.46 cm), ear length (12.17 cm), kernels ear<sup>-1</sup> (434.83), kernel yield (2095.7 kg·ha<sup>-1</sup>), total kernel weight (350.75 kg·ha<sup>-1</sup>), biomass yield (4015.3 kg·ha<sup>-1</sup>) and HI (37.31) were recorded under the treatment of UREA + PM, and followed by UREA + FYM. Besides, the applications of organic manure in combination with nitrogen significantly increased yield and its components. Application of 50% of N and 50% of poultry manure produced higher performance for the traits of plant height, ear length, kernels ear<sup>-1</sup>, total kernels weight ear<sup>-1</sup>, kernel yield, and biomass yield.
文摘Wild relatives possess potential genetic diversity for maize (<i><span style="font-family:Verdana;">Zea mays</span></i><span style="font-family:Verdana;"> L.) improvement. Characterization of maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> introgression lines (ILs) is of great value to diversify the genetic base and improve the maize germplasm. Four maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> IL generations, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;"> BC1, BC2, BC3, and RIL, were constructed under the elite inbred background of 48-2, elite inbred line that is widely used in maize breeding in Southwestern China, and were phenotyped in different years and genotyped with 56110 SNPs. The results indicated that 48-2 had higher phenotypic performances than all the characterized ILs on most of the agronomic traits. Compared with other ILs, BC2 individuals exhibited more similar performance to 48-2 on most traits and possessed the highest kernel ratio (66.5%). Population structure and principal component analysis indicated that BC3 individuals gathered closer to 48-2 and exhibited the lowest </span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;">-introgression frequency (0.50%), while BC2 (29.06%) and RIL (18.52%) showed higher introgression frequency. The high level of genetic diversity observed in the maize-</span><i><span style="font-family:Verdana;">mexicana</span></i><span style="font-family:Verdana;"> ILs demonstrated that </span><i><span style="font-family:Verdana;">Z</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">mays</span></i><span style="font-family:Verdana;"> ssp. </span><i><span style="font-family:Verdana;">mexicana </span></i><span style="font-family:Verdana;">can serve as a potential source for the enrichment of maize germplasm.</span>
