In this study, tumor microenvironment and related pathways play an important role in the EMT process of bile duct tumors</span></span><span><span><span style="font-family:""&g...In this study, tumor microenvironment and related pathways play an important role in the EMT process of bile duct tumors</span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">to analyze the role of EMT in the occurrence and development of cholangiolithiasis associated bile duct carcinoma</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> and</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> investigate the expression of EMT in intrahepatic cholangiolithiasis associated cholangiocarcinoma and its clinical significance.展开更多
<strong>Background:</strong> By studying the expression of epithelial-mesenchymal transition regulators in cholangiocarcinoma and intrahepatic duct stones, the correlation between the expression of epithel...<strong>Background:</strong> By studying the expression of epithelial-mesenchymal transition regulators in cholangiocarcinoma and intrahepatic duct stones, the correlation between the expression of epithelial-mesenchymal transition regulators and cholangiocarcinoma was revealed. <strong>Objective:</strong> The objective is to investigate the correlation between the expression of epithelial-mesenchymal transition (EC) regulatory factors and cholangiocarcinoma in patients with intrahepatic duct stones and cholangiocarcinoma, to investigate the relationship between clinicopathological features and prognosis, and to observe the expression of molecular markers of epithelial-mesenchymal transition (EMT) in intrahepatic duct stones and bile duct carcinoma. <strong>Methods: </strong>Twenty cases of primary cholangiocarcinoma, 20 cases of intrahepatic cholangiolithiasis complicated with cholangiocarcinoma, and 20 cases of intrahepatic cholangiolithiasis specimens were collected from the Fourth People’s Hospital and the friendly medical unit of Haikou. Immunohistochemistry was used to detect the expression differences of EMT-related molecular markers Twisit1, Twisit2, E-cadherin, N-cadherin, and Vimentin in paraffin sections of normal intrahepatic bile duct tissues and patients with intrahepatic duct stones and cholangiocarcinoma. <strong>Results:</strong> Immunohistochemical staining revealed epithelial-mesenchymal transition (EMT) in intrahepatic cholangiocarcinoma tissue, intrahepatic cholangiolithiasis with cholangiocarcinoma, intrahepatic cholangiolithiasis with normal intrahepatic cholangiolithiasis, such as Sit1, Twisit2, E-cadherin, N-cadherin, and Vimentin proteins were different. The expression of E-cadherin was decreased in cholangiocarcinoma tissue and intrahepatic cholangiolithiocarcinoma combined with cholangiocarcinoma (P < 0.05), while the expression of N-cadherin and Vimentin was up-regulated (P < 0.05). The expression of Twisit1 and Twisit2 had no difference (P > 0.05). There was no difference in the ex展开更多
In 2006, Duman et al. proposed “Neurotrophic Theory of Depression” [1]. According to the hypothesis, stress leads to a decrease in the expression of neurotrophic factors such as Brain-derived neurotrophic factor (BD...In 2006, Duman et al. proposed “Neurotrophic Theory of Depression” [1]. According to the hypothesis, stress leads to a decrease in the expression of neurotrophic factors such as Brain-derived neurotrophic factor (BDNF) in the limbic structure, and antidepressant therapy can partially reverse the effect caused by stress. The reduction of BDNF and other neurotrophic factors promotes the atrophy of certain brain structures, especially the hippocampus and prefrontal cortex, while antidepressant treatment increases the level of BDNF in the brain, and improves synaptic plasticity and neuronal survival in related brain regions. Neurotrophic factors are a class of molecules that act on the nervous system and play an important role in maintaining cell function. They can regulate the growth, survival, differentiation and cell cycle of nerve cells. There is a hypothesis of neuroendocrine dysfunction in the neurobiochemical mechanism of depression, which is mainly the abnormal activity of the hypothalamic-pituitary-adrenal axis (HPA) and hypothalamic-pituitary-thyroid axis (HPT). This article reviews the related research on depression and brain-derived neurotrophic factors in order to guide clinical research and treatment.展开更多
Objective: To analyze the correlation between Val66Met gene of brain-derived neurotrophic factor (BDNF) and papillary thyroid carcinoma (PTC) complicated with depression. To evaluate the clinical value of papillary th...Objective: To analyze the correlation between Val66Met gene of brain-derived neurotrophic factor (BDNF) and papillary thyroid carcinoma (PTC) complicated with depression. To evaluate the clinical value of papillary thyroid carcinoma. Methods: Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were used to assess the correlation of BDNF in the blood of patients with depressive disorder of thyroid papillary carcinoma using polymerase chain reaction PCR. The relationship between BDNF gene polymorphism and the incidence of thyroid papillary carcinoma was analyzed, and the susceptibility factors of thyroid papillary carcinoma complicated with depression were explored. Results: Compared with normal control group, T3 and T4 of PTC in non-depressed group were decreased, while TSH and TPOAb were increased (P < 0.05). Compared with normal control group, T3, T4 and TPOAb were increased and TSH was decreased in PTC depression group (P < 0.05). PTC depression score was higher than that of healthy control group (P < 0.05), and PTC combined depression score was higher than that of normal control group (P < 0.05). The depression rate of PTC combined with depression was 86.7%, which was higher than that of other groups (P < 0.05). The distribution of RS6265 locus genotype of BDNF gene was significantly different between PTC with depression group and PTC without depression group (P Conclusion: There is a significant difference between PTC with depression and PTC without depression, which is related to SNP rs6265 of BDNF gene. AG and GG are both risk sites of PTC with depression, and GG type is more prone to PTC with depression.展开更多
文摘In this study, tumor microenvironment and related pathways play an important role in the EMT process of bile duct tumors</span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">to analyze the role of EMT in the occurrence and development of cholangiolithiasis associated bile duct carcinoma</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> and</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> investigate the expression of EMT in intrahepatic cholangiolithiasis associated cholangiocarcinoma and its clinical significance.
文摘<strong>Background:</strong> By studying the expression of epithelial-mesenchymal transition regulators in cholangiocarcinoma and intrahepatic duct stones, the correlation between the expression of epithelial-mesenchymal transition regulators and cholangiocarcinoma was revealed. <strong>Objective:</strong> The objective is to investigate the correlation between the expression of epithelial-mesenchymal transition (EC) regulatory factors and cholangiocarcinoma in patients with intrahepatic duct stones and cholangiocarcinoma, to investigate the relationship between clinicopathological features and prognosis, and to observe the expression of molecular markers of epithelial-mesenchymal transition (EMT) in intrahepatic duct stones and bile duct carcinoma. <strong>Methods: </strong>Twenty cases of primary cholangiocarcinoma, 20 cases of intrahepatic cholangiolithiasis complicated with cholangiocarcinoma, and 20 cases of intrahepatic cholangiolithiasis specimens were collected from the Fourth People’s Hospital and the friendly medical unit of Haikou. Immunohistochemistry was used to detect the expression differences of EMT-related molecular markers Twisit1, Twisit2, E-cadherin, N-cadherin, and Vimentin in paraffin sections of normal intrahepatic bile duct tissues and patients with intrahepatic duct stones and cholangiocarcinoma. <strong>Results:</strong> Immunohistochemical staining revealed epithelial-mesenchymal transition (EMT) in intrahepatic cholangiocarcinoma tissue, intrahepatic cholangiolithiasis with cholangiocarcinoma, intrahepatic cholangiolithiasis with normal intrahepatic cholangiolithiasis, such as Sit1, Twisit2, E-cadherin, N-cadherin, and Vimentin proteins were different. The expression of E-cadherin was decreased in cholangiocarcinoma tissue and intrahepatic cholangiolithiocarcinoma combined with cholangiocarcinoma (P < 0.05), while the expression of N-cadherin and Vimentin was up-regulated (P < 0.05). The expression of Twisit1 and Twisit2 had no difference (P > 0.05). There was no difference in the ex
文摘In 2006, Duman et al. proposed “Neurotrophic Theory of Depression” [1]. According to the hypothesis, stress leads to a decrease in the expression of neurotrophic factors such as Brain-derived neurotrophic factor (BDNF) in the limbic structure, and antidepressant therapy can partially reverse the effect caused by stress. The reduction of BDNF and other neurotrophic factors promotes the atrophy of certain brain structures, especially the hippocampus and prefrontal cortex, while antidepressant treatment increases the level of BDNF in the brain, and improves synaptic plasticity and neuronal survival in related brain regions. Neurotrophic factors are a class of molecules that act on the nervous system and play an important role in maintaining cell function. They can regulate the growth, survival, differentiation and cell cycle of nerve cells. There is a hypothesis of neuroendocrine dysfunction in the neurobiochemical mechanism of depression, which is mainly the abnormal activity of the hypothalamic-pituitary-adrenal axis (HPA) and hypothalamic-pituitary-thyroid axis (HPT). This article reviews the related research on depression and brain-derived neurotrophic factors in order to guide clinical research and treatment.
文摘Objective: To analyze the correlation between Val66Met gene of brain-derived neurotrophic factor (BDNF) and papillary thyroid carcinoma (PTC) complicated with depression. To evaluate the clinical value of papillary thyroid carcinoma. Methods: Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were used to assess the correlation of BDNF in the blood of patients with depressive disorder of thyroid papillary carcinoma using polymerase chain reaction PCR. The relationship between BDNF gene polymorphism and the incidence of thyroid papillary carcinoma was analyzed, and the susceptibility factors of thyroid papillary carcinoma complicated with depression were explored. Results: Compared with normal control group, T3 and T4 of PTC in non-depressed group were decreased, while TSH and TPOAb were increased (P < 0.05). Compared with normal control group, T3, T4 and TPOAb were increased and TSH was decreased in PTC depression group (P < 0.05). PTC depression score was higher than that of healthy control group (P < 0.05), and PTC combined depression score was higher than that of normal control group (P < 0.05). The depression rate of PTC combined with depression was 86.7%, which was higher than that of other groups (P < 0.05). The distribution of RS6265 locus genotype of BDNF gene was significantly different between PTC with depression group and PTC without depression group (P Conclusion: There is a significant difference between PTC with depression and PTC without depression, which is related to SNP rs6265 of BDNF gene. AG and GG are both risk sites of PTC with depression, and GG type is more prone to PTC with depression.
