CARDC(China Aerodynamics Research and Development Center)has designed and developed various kinds of axial fans for specific wind tunnels in the previous decade.This paper not only gives summary about it,but also talk...CARDC(China Aerodynamics Research and Development Center)has designed and developed various kinds of axial fans for specific wind tunnels in the previous decade.This paper not only gives summary about it,but also talks its future development.First,the contents are presented about high aerodynamic performance low-noise fan,including the methods of designing fan and ways of controlling noise generation and propagation,and how to reconcile the difficulties between noise control and fan efficiency raise.Then a simplified method of adjusting fan-blade angle during commissioning test is proposed and validated in practice.Contrary to usually used method,this simplified method can be implemented without using any test instruments of the fan,thus significantly reducing the commissioning test time of the fan.This is followed by the design of high pressure low Reynolds number axial fan.By using the method discussed in the paper,the restriction of Ma at blade tip not higher than 0.5 is relaxed.Accordingly,the pressure ratio obtained through one stage fan was raised above to 1.08,realizing the design target of Ma as 0.7 of the air in the test section.In addition,the topics about fan array,low hub-tip ratio fan and high pressure fan have been briefly covered for the future work.展开更多
目的构建沉默信息调节因子(silent information regulaor,Sir)样蛋白1(Sirtuin 1,SIRT1)的特异性短发夹RNA(shRNA)慢病毒载体,获得敲低SIRT1的肝癌细胞系,以探讨其对肝癌细胞的增殖和耐药敏感性的作用。方法设计针对SIRT1靶点特异性的...目的构建沉默信息调节因子(silent information regulaor,Sir)样蛋白1(Sirtuin 1,SIRT1)的特异性短发夹RNA(shRNA)慢病毒载体,获得敲低SIRT1的肝癌细胞系,以探讨其对肝癌细胞的增殖和耐药敏感性的作用。方法设计针对SIRT1靶点特异性的干涉序列,连接到经HpaⅠ和XhoⅠ双酶切的pSicoR-GFP载体,慢病毒包装293T产生病毒,感染肝癌细胞,建立肝癌细胞SIRT1低表达的稳定株。利用实时定量PCR检测SIRT1的干涉效果;通过平板克隆形成实验、CCK8细胞增殖实验检测SIRT1被干涉后对肝癌细胞增殖能力的影响;通过细胞耐药敏感性实验及实时定量PCR检测耐药基因的表达,考察SIRT1干涉对肝癌细胞耐药敏感性的影响。结果实时定量PCR实验证明该慢病毒干涉载体能显著抑制肝癌细胞中SIRT1的表达,SIRT1干涉可抑制肝癌细胞的增殖能力,下调耐药基因的表达,增强肝癌细胞的药物敏感性。结论 SIRT1抑制肝癌细胞耐药性。展开更多
文摘CARDC(China Aerodynamics Research and Development Center)has designed and developed various kinds of axial fans for specific wind tunnels in the previous decade.This paper not only gives summary about it,but also talks its future development.First,the contents are presented about high aerodynamic performance low-noise fan,including the methods of designing fan and ways of controlling noise generation and propagation,and how to reconcile the difficulties between noise control and fan efficiency raise.Then a simplified method of adjusting fan-blade angle during commissioning test is proposed and validated in practice.Contrary to usually used method,this simplified method can be implemented without using any test instruments of the fan,thus significantly reducing the commissioning test time of the fan.This is followed by the design of high pressure low Reynolds number axial fan.By using the method discussed in the paper,the restriction of Ma at blade tip not higher than 0.5 is relaxed.Accordingly,the pressure ratio obtained through one stage fan was raised above to 1.08,realizing the design target of Ma as 0.7 of the air in the test section.In addition,the topics about fan array,low hub-tip ratio fan and high pressure fan have been briefly covered for the future work.
基金国家重大科研仪器研制项目(No.51427804)国家重点研发计划项目(No.2016YFC0801402+3 种基金No.2016YFC0801404)supported by the National Major Scientific Instruments Development Project(51427804)the State Key Project of Research and Development Plan(2016YFC08014022016YFC0801404)
文摘目的构建沉默信息调节因子(silent information regulaor,Sir)样蛋白1(Sirtuin 1,SIRT1)的特异性短发夹RNA(shRNA)慢病毒载体,获得敲低SIRT1的肝癌细胞系,以探讨其对肝癌细胞的增殖和耐药敏感性的作用。方法设计针对SIRT1靶点特异性的干涉序列,连接到经HpaⅠ和XhoⅠ双酶切的pSicoR-GFP载体,慢病毒包装293T产生病毒,感染肝癌细胞,建立肝癌细胞SIRT1低表达的稳定株。利用实时定量PCR检测SIRT1的干涉效果;通过平板克隆形成实验、CCK8细胞增殖实验检测SIRT1被干涉后对肝癌细胞增殖能力的影响;通过细胞耐药敏感性实验及实时定量PCR检测耐药基因的表达,考察SIRT1干涉对肝癌细胞耐药敏感性的影响。结果实时定量PCR实验证明该慢病毒干涉载体能显著抑制肝癌细胞中SIRT1的表达,SIRT1干涉可抑制肝癌细胞的增殖能力,下调耐药基因的表达,增强肝癌细胞的药物敏感性。结论 SIRT1抑制肝癌细胞耐药性。
