The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains ...The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains obscure.In this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of9 959 autosomal copy number variations(CNVs) were identified through selection signal analysis in 127 goat genomes.Specifically,we confirmed that the highest signal CNV(HSV) was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp) with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats.The expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA sequencing.Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs) and their overexpression significantly increased the expression of SAA3.The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.展开更多
High-purity porous Ti3SiC2 with a porosity of 54.3%was prepared by reactive synthesis and its oxidation behavior was evaluated under air in the temperature range from 400 to 1000°C.Thermogravimetric analysis and ...High-purity porous Ti3SiC2 with a porosity of 54.3%was prepared by reactive synthesis and its oxidation behavior was evaluated under air in the temperature range from 400 to 1000°C.Thermogravimetric analysis and differential scanning calorimetry(TG-DSC),scanning electron microscope(SEM),X-ray diffractometometry(XRD),energy dispersive spectrometer(EDS),Raman spectrum,BET surface area analysis,and pore-parameter testing were applied to the studies of the oxidation kinetics,phase composition,micro morphology,and porous structure parameters of porous Ti3SiC2 before and after oxidation.The results showed that the formation of TiO2 oxidized products with different modifications was the primary factor influencing the oxidation resistance and structural stability of porous Ti3SiC2.Cracks were observed in the samples oxidized in the full temperature range of 400-1000°C because of the growth stress and thermal stress.At 400-600°C,anomalous oxidation with higher kinetics and the aberrant decrement in pore size and permeability were attributed to the occurrence of severe cracking caused by the formation of anatase TiO2.At raised temperatures over 600°C,the cracking phenomena were alleviated by the formation of rutile TiO2,but the outward growth of the oxide scales detrimentally decreased the connectivity of porous Ti3SiC2.展开更多
Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant ...Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.展开更多
Genetic diversity,population structure,and population expansion of goats worldwide(4165 individuals from 196 breeds)were analyzed using published mitochondrial DNA(mtDNA)D_loop hypervariable region sequences.Results s...Genetic diversity,population structure,and population expansion of goats worldwide(4165 individuals from 196 breeds)were analyzed using published mitochondrial DNA(mtDNA)D_loop hypervariable region sequences.Results showed that 2409 haplotypes and 301 polymorphic sites were present within the 401-bp length D_loop region,the nucleotide diversity(Pi)was 0.03471,and the haplotype diversity(Hd)was 0.9983.Phylogenetic analysis revealed that 98.92%of haplotypes were divided into six obvious clusters,consistent with the classification of the known mitochondrial haplogroups of goats.Haplogroup A accounted for the largest proportion(86%).Interestingly,two unknown divisions(Unknown I and Unknown II)were discovered from goats in Southwest China,suggesting that Southwest China has unique maternal haplogroups.Analysis of molecular variance(AMOVA)and the average number of pairwise differences between populations(PiXY)indicated that geographical variation was small but significant.Neutrality tests(Tajima’s D and Fu’s FS tests)and mismatch distribution showed that haplogroups B,C,and G had expansion histories.In addition,the phylogenetic relationship between domestic and wild goats suggested that Capra aegagrus is the most likely wild ancestor and may have participated in the domestication of ancestral populations of A,B,C,and F haplogroups.A meta-analysis on the mtDNA sequences of goats from international databases was conducted to analyze goats’genetic diversity,population structure,and matrilineal system evolution worldwide.The results may help further understand the domestication history and gene flow of goats worldwide.展开更多
A binary continuum model for dendritic solidification transport phenomena and corresponding numerical algorithm for the strong nonlinear coupling of T-fS-CL were extended to multicomponent alloys solidified under cond...A binary continuum model for dendritic solidification transport phenomena and corresponding numerical algorithm for the strong nonlinear coupling of T-fS-CL were extended to multicomponent alloys solidified under condition of Biot≤0.1. Based on the extended model/algorithm, a method considering heat transfer was proposed to predict the solidification paths and microsegregation of alloys solidified under the same condition. The new algorithm and method were closely coupled with the commercial Thermo-Calc package via its TQ6-interface codes for instantaneous determination of the related thermodynamic data at each calculation time step. The sample simulation performed on an Al-2Si-3Mg alloy system indicates the availability and reliability of the model/algorithm and the proposed method for predicting solidification paths and microsegregation. Computional and experimental investigations on an Al-5.17Cu-2.63Si ternary alloy were conducted, and a reasonable agreement between the computation and experiment was obtained.展开更多
Massive vanadium additions as hard phases in powder metallurgy high-speed steels(PM HSS)lead to higher cost and bad machinability.In this study,ultrahigh alloy PM HSS with CPM121(10W-5Mo-4Cr-10V-9Co,wt.%)as the basic ...Massive vanadium additions as hard phases in powder metallurgy high-speed steels(PM HSS)lead to higher cost and bad machinability.In this study,ultrahigh alloy PM HSS with CPM121(10W-5Mo-4Cr-10V-9Co,wt.%)as the basic composition,was directly compacted and activation sintered with near-full density(>99.0%)using pre-oxidized and ball-mixed element and carbide powders.Niobium-alloyed steels(w(V)+w(Nb)=10 wt.%)show higher hardness and wear resistance,superior secondary-hardening ability and temper resistance.But excess niobium addition(>5 wt.%)leads to coarsened carbides and deteriorated toughness.EPMA results proved that niobium tends to distribute in MC carbides and forces element W to form M6C and WC carbides.Further,the role of rotary forging on properties of niobium-alloyed steels(S3)was researched.After rotary forging with deformation of 40%,the bending strength and fracture toughness of niobium-alloyed steels could be further improved by 20.74%and 43.86%compared with those of sample S3 without rotary forging,respectively.展开更多
基金supported by the National Natural Science Foundation of China (32272834)。
文摘The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains obscure.In this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of9 959 autosomal copy number variations(CNVs) were identified through selection signal analysis in 127 goat genomes.Specifically,we confirmed that the highest signal CNV(HSV) was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp) with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats.The expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA sequencing.Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs) and their overexpression significantly increased the expression of SAA3.The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.
基金Project supported by the National High-Tech R&D Program(863 Program)of China(No.2014AA0415011)the Research Fund for the Doctoral Program of Higher Education of China(No.2014QD02S)+1 种基金the Research Fund for the Doctoral Program of Higher Education of China(No.2013QD03S)the Fundamental Research Funds for the Central Universities(No.3122015C015),China
基金Projects(51801183,51634006,51504296,51604305)supported by the National Natural Science Foundation of China
文摘High-purity porous Ti3SiC2 with a porosity of 54.3%was prepared by reactive synthesis and its oxidation behavior was evaluated under air in the temperature range from 400 to 1000°C.Thermogravimetric analysis and differential scanning calorimetry(TG-DSC),scanning electron microscope(SEM),X-ray diffractometometry(XRD),energy dispersive spectrometer(EDS),Raman spectrum,BET surface area analysis,and pore-parameter testing were applied to the studies of the oxidation kinetics,phase composition,micro morphology,and porous structure parameters of porous Ti3SiC2 before and after oxidation.The results showed that the formation of TiO2 oxidized products with different modifications was the primary factor influencing the oxidation resistance and structural stability of porous Ti3SiC2.Cracks were observed in the samples oxidized in the full temperature range of 400-1000°C because of the growth stress and thermal stress.At 400-600°C,anomalous oxidation with higher kinetics and the aberrant decrement in pore size and permeability were attributed to the occurrence of severe cracking caused by the formation of anatase TiO2.At raised temperatures over 600°C,the cracking phenomena were alleviated by the formation of rutile TiO2,but the outward growth of the oxide scales detrimentally decreased the connectivity of porous Ti3SiC2.
基金supported by the National Natural Science Foundations of China (31402033, U1603232)the Special Fund for Basic Scientific Research of Institute of Animal Sciences,the Chinese Academy of Agricultural Sciences (2017ywf-zd-11, Y2017JC03)the Agricultural Science and Technology Innovation Program of China (ASTIPIAS01)
文摘Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2(RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an i TRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins(DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes(biological adhesion(P=4.07×10–17) and cell adhesion(P=3.7×10–16)), multicellular organismal process(single-multicellular organism process(P=2.06×10–11) and multicellular organismal process(P=2.29×10–11)) and extracellular processes(extracellular matrix organization(P=4.77×10–16) and extracellular structure organization(P=4.93×10–16)). Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that extracellular matrix(ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.
基金funded by the Chongqing Natural Science Foundation, China (cstc2021jcyj-msxm X0013)the National Natural Science Foundation of China (31172195)
文摘Genetic diversity,population structure,and population expansion of goats worldwide(4165 individuals from 196 breeds)were analyzed using published mitochondrial DNA(mtDNA)D_loop hypervariable region sequences.Results showed that 2409 haplotypes and 301 polymorphic sites were present within the 401-bp length D_loop region,the nucleotide diversity(Pi)was 0.03471,and the haplotype diversity(Hd)was 0.9983.Phylogenetic analysis revealed that 98.92%of haplotypes were divided into six obvious clusters,consistent with the classification of the known mitochondrial haplogroups of goats.Haplogroup A accounted for the largest proportion(86%).Interestingly,two unknown divisions(Unknown I and Unknown II)were discovered from goats in Southwest China,suggesting that Southwest China has unique maternal haplogroups.Analysis of molecular variance(AMOVA)and the average number of pairwise differences between populations(PiXY)indicated that geographical variation was small but significant.Neutrality tests(Tajima’s D and Fu’s FS tests)and mismatch distribution showed that haplogroups B,C,and G had expansion histories.In addition,the phylogenetic relationship between domestic and wild goats suggested that Capra aegagrus is the most likely wild ancestor and may have participated in the domestication of ancestral populations of A,B,C,and F haplogroups.A meta-analysis on the mtDNA sequences of goats from international databases was conducted to analyze goats’genetic diversity,population structure,and matrilineal system evolution worldwide.The results may help further understand the domestication history and gene flow of goats worldwide.
基金Projects (2008112042) supported by the Open Project of State Key Laboratory of Powder Metallurgy of Central South University, ChinaProjects (50771041, 50801019) supported by the National Natural Science Foundation of ChinaProject (2011CB610406) supported by the National Basic Research Program of China
文摘A binary continuum model for dendritic solidification transport phenomena and corresponding numerical algorithm for the strong nonlinear coupling of T-fS-CL were extended to multicomponent alloys solidified under condition of Biot≤0.1. Based on the extended model/algorithm, a method considering heat transfer was proposed to predict the solidification paths and microsegregation of alloys solidified under the same condition. The new algorithm and method were closely coupled with the commercial Thermo-Calc package via its TQ6-interface codes for instantaneous determination of the related thermodynamic data at each calculation time step. The sample simulation performed on an Al-2Si-3Mg alloy system indicates the availability and reliability of the model/algorithm and the proposed method for predicting solidification paths and microsegregation. Computional and experimental investigations on an Al-5.17Cu-2.63Si ternary alloy were conducted, and a reasonable agreement between the computation and experiment was obtained.
基金Projects(51771237,51704257)supported by the National Natural Science Foundation of ChinaProject(2019JJ60019)supported by the Joint Fund of Hunan Province,ChinaProject(17QDZ25)supported by the School Level Fund of Xiangtan University,China。
文摘Massive vanadium additions as hard phases in powder metallurgy high-speed steels(PM HSS)lead to higher cost and bad machinability.In this study,ultrahigh alloy PM HSS with CPM121(10W-5Mo-4Cr-10V-9Co,wt.%)as the basic composition,was directly compacted and activation sintered with near-full density(>99.0%)using pre-oxidized and ball-mixed element and carbide powders.Niobium-alloyed steels(w(V)+w(Nb)=10 wt.%)show higher hardness and wear resistance,superior secondary-hardening ability and temper resistance.But excess niobium addition(>5 wt.%)leads to coarsened carbides and deteriorated toughness.EPMA results proved that niobium tends to distribute in MC carbides and forces element W to form M6C and WC carbides.Further,the role of rotary forging on properties of niobium-alloyed steels(S3)was researched.After rotary forging with deformation of 40%,the bending strength and fracture toughness of niobium-alloyed steels could be further improved by 20.74%and 43.86%compared with those of sample S3 without rotary forging,respectively.
