Sex determination has been studied in the model lepidopteran species Bombyx mori, but it remains poorly understood in lepidopteran pests. In the present study, we identified and characterized the Masculinizer (Masc) g...Sex determination has been studied in the model lepidopteran species Bombyx mori, but it remains poorly understood in lepidopteran pests. In the present study, we identified and characterized the Masculinizer (Masc) gene in a Noctuidae pest species, Agrotis ipsilon. Sequence analysis revealed that AiMasc encodes a protein of 658 amino acids that has two CCCH-type zinc finger domains and two conserved cysteine residues (Cys-277 and Cys-280). We assessed the masculinizing activity AiMasc in BmN cells and found that/z'Masc induced expression of the male-specific doublesex isoform. Disruption of Masc via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) in A. ipsilon caused abnormalities in abdominal segments and external genitalia, resulting in male-specific sterility. These results suggest that Masc participates in the process of sex determination in A. ipsilon. Successful identification of sex-determination gene in a pest species may enable the development of novel genetic approaches for pest control.展开更多
The growth and development of metabolous insects are mainly regulated by ecdysone and juvenile hormone.As a member of the low-density lipoprotein receptor(LDLR)family,megalin(mgl)is involved in the lipoprotein transpo...The growth and development of metabolous insects are mainly regulated by ecdysone and juvenile hormone.As a member of the low-density lipoprotein receptor(LDLR)family,megalin(mgl)is involved in the lipoprotein transport of cholesterol which is an essential precursor for the synthesis of ecdysone.Despite extensive studies in mammals,the function of mgl is still largely unknown in insects.In this study,we characterize the function of mgl in the silkworm Bombyx mori,the model species of Lepidoptera.We find that mgl is broadly present in the genomes of lepidopteran species and evolved with divergence between lepidopterans and Drosophila.The expression pattern suggests a ubiquitous role of mgl in the growth and development in the silkworm.We further perform clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based mutagenesis of Bmmgl and find that both the development and the silk production of the silkworm are seriously affected by the disruption of Bmmgl.Our results not only explore the function of mgl in Lepidoptera but also add to our understanding of how cholesterol metabolism is involved in the development of insects.展开更多
非特异性下腰痛(nonspecific low back pain,NLBP)广义包括腰肌劳损、腰背肌筋膜炎、第3腰椎横突综合征、急性腰扭伤、梨状肌综合征、骶髂关节炎、肌纤维组织炎等多种急慢性腰部病变所致的腰痛及其导致的运动功能障碍。笔者采用浮针扫散...非特异性下腰痛(nonspecific low back pain,NLBP)广义包括腰肌劳损、腰背肌筋膜炎、第3腰椎横突综合征、急性腰扭伤、梨状肌综合征、骶髂关节炎、肌纤维组织炎等多种急慢性腰部病变所致的腰痛及其导致的运动功能障碍。笔者采用浮针扫散"腰五穴"配合再灌注活动治疗非特异性下腰痛23例,现报道如下。展开更多
基金financially supported by the Key Research and Development Program of China(No.2022YFB2404102)the National Natural Science Foundation of China(Nos.51971093,52171158)the Key Research and Development Program of Shandong Province,China(Nos.2021ZLGX01,2022CXGC020308)。
基金partly supported by the National Natural Science Foundation of China (Nos. 52174362, 51975207)the Xiangtan Special Project for Building a National Innovative City,China (No. CG-YB20221043)the Yancheng “Talent Plan of Yellow Sea Pearl” for Leading Talent Project,China。
基金supported by the Hunan Provincial Natural Science Foundation,China(No.2023JJ40626)Science and Technology Innovation Program of Hunan Province,China(No.2020RC2080)the National Natural Science Foundation of China(No.51971190)。
基金the National Basic Research Program of China (2015CB755703)the National Science Foundation of China (31522053,31501648,3142010391 & and 91631103)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11010600).
文摘Sex determination has been studied in the model lepidopteran species Bombyx mori, but it remains poorly understood in lepidopteran pests. In the present study, we identified and characterized the Masculinizer (Masc) gene in a Noctuidae pest species, Agrotis ipsilon. Sequence analysis revealed that AiMasc encodes a protein of 658 amino acids that has two CCCH-type zinc finger domains and two conserved cysteine residues (Cys-277 and Cys-280). We assessed the masculinizing activity AiMasc in BmN cells and found that/z'Masc induced expression of the male-specific doublesex isoform. Disruption of Masc via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) in A. ipsilon caused abnormalities in abdominal segments and external genitalia, resulting in male-specific sterility. These results suggest that Masc participates in the process of sex determination in A. ipsilon. Successful identification of sex-determination gene in a pest species may enable the development of novel genetic approaches for pest control.
基金supported by the National Science Foundation of China(grant numbers 32021001 and 32000339)Postgraduate Research&Practice Innovation Program of Jiangsu Province.
文摘The growth and development of metabolous insects are mainly regulated by ecdysone and juvenile hormone.As a member of the low-density lipoprotein receptor(LDLR)family,megalin(mgl)is involved in the lipoprotein transport of cholesterol which is an essential precursor for the synthesis of ecdysone.Despite extensive studies in mammals,the function of mgl is still largely unknown in insects.In this study,we characterize the function of mgl in the silkworm Bombyx mori,the model species of Lepidoptera.We find that mgl is broadly present in the genomes of lepidopteran species and evolved with divergence between lepidopterans and Drosophila.The expression pattern suggests a ubiquitous role of mgl in the growth and development in the silkworm.We further perform clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based mutagenesis of Bmmgl and find that both the development and the silk production of the silkworm are seriously affected by the disruption of Bmmgl.Our results not only explore the function of mgl in Lepidoptera but also add to our understanding of how cholesterol metabolism is involved in the development of insects.
文摘非特异性下腰痛(nonspecific low back pain,NLBP)广义包括腰肌劳损、腰背肌筋膜炎、第3腰椎横突综合征、急性腰扭伤、梨状肌综合征、骶髂关节炎、肌纤维组织炎等多种急慢性腰部病变所致的腰痛及其导致的运动功能障碍。笔者采用浮针扫散"腰五穴"配合再灌注活动治疗非特异性下腰痛23例,现报道如下。
