Background:Human leukocyte antigen-identical sibling donor(ISD)-hematopoietic stem cell transplantation(SCT)is a potentially curative treatment for high-risk pediatric acute myeloid leukemia(AML).A haploidentical dono...Background:Human leukocyte antigen-identical sibling donor(ISD)-hematopoietic stem cell transplantation(SCT)is a potentially curative treatment for high-risk pediatric acute myeloid leukemia(AML).A haploidentical donor(HID)is readily available to almost all children.Previous studies have demonstrated that patients with HID-SCT had similar outcomes compared to ISD-SCT for pediatric and adult AML.However,the role of HID-SCT in high-risk pediatric AML is unclear.Methods:To compare the overall survival of high-risk AML children who underwent either HID-SCT or ISD-SCT,we analyzed 179 cases of high-risk AML patients under 18 years of age treated with either ISD-SCT(n=23)or HID-SCT(n=156).Granulocyte colony-stimulating factor plus anti-thymocyte globulin-based regimens were used for HID-SCT.We also analyzed the subgroup data of AML patients at first complete remission(CR1)before SCT with known cytogenetic risk.Results:The numbers of adverse cytogenetic risk recipients were 8(34.8%)and 13(18.8%)in the ISD-SCT group and the HID-SCT group,and the number of patients with disease status beyond CR1 were 6(26.1%)and 14(20.3%)in the two groups.The cumulative rates of grades II-IV acute graft-versus-host disease(GVHD)were 13.0%in the ISD-SCT group and 34.8%in the HID-SCT group(P=0.062),with a three-year cumulative rates of chronic GVHD at 14.1%and 34.9%,respectively(P=0.091).The relapse rate in the ISD-SCT group was significantly higher than that in the HID-SCT group(39.1%vs.16.4%,P=0.027);with non-relapse mortality at 0.0%and 10.6%(P=0.113),respectively.The three-year overall survival rates were 73.0%for the ISD-SCT group and 74.6%for the HID-SCT group(P=0.689).In subgroup analysis,the three-year relapse rate in the ISD-SCT group was higher than that in the HID-SCT group(50.0%vs.9.2%,P=0.001)and the three-year DFS in the ISD-SCT group(50.0%)was lower than that in the HID-SCT group(81.2%)(P=0.021).Conclusions:Unmanipulated HID-SCT achieved DFS and OS outcomes comparable to those of ISD-SCT for high-risk pediatric AML patients wi展开更多
基于溶血曼氏杆菌(Mannheimia haemolytica,M.haemolytica)单拷贝基因sodA,通过优化反应条件和体系,建立了溶血曼氏杆菌ddPCR(droplet digital polymerase chain reaction,ddPCR)定量检测方法。结果显示,所建立的溶血曼氏杆菌ddPCR方法...基于溶血曼氏杆菌(Mannheimia haemolytica,M.haemolytica)单拷贝基因sodA,通过优化反应条件和体系,建立了溶血曼氏杆菌ddPCR(droplet digital polymerase chain reaction,ddPCR)定量检测方法。结果显示,所建立的溶血曼氏杆菌ddPCR方法特异性好;检出限(limit of detection,LOD)和定量值(limit of quantification,LOQ)均为170 CFU/m L,3个平行检测的变异系数均小于25%,重复性良好。针对系列稀释的溶血曼氏杆菌纯菌液,ddPCR法能够实现对溶血曼氏杆菌的准确定量,与平板计数结果偏差小于4%。运用所建立的ddPCR方法对15份牛鼻拭子样品进行检测,结果在10份样品中检出溶血曼氏杆菌,含量在3.07×10^(2)~9.52×10^(4)CFU/mL之间。本研究所建立的ddPCR方法适用于临床鼻拭子样品中溶血曼氏杆菌的精准定量分析,可为牛群中溶血曼氏杆菌的监测和治疗提供有效的技术支持。展开更多
A low-voltage-driven digital-droplet-transporting chip with an open structure is designed,fabricated and characterized.The digital microfluidic chip is fabricated by the silicon planar process.Using only a single elec...A low-voltage-driven digital-droplet-transporting chip with an open structure is designed,fabricated and characterized.The digital microfluidic chip is fabricated by the silicon planar process.Using only a single electrode panel,the droplet on the chip can be manipulated by electrostatic force under a dc driving voltage.The actuation principle is proposed and verified by the experiment.The experimental results show that the minimum driving voltage decreases as the thickness of the dielectric layer decreases.The driving voltage for a 3µL deionized(DI)water droplet is reduced to 15 V in air and 13.5 V in oil by employing a thin dielectric layer of 600 nm with a high dielectric constant and a coating hydrophobic layer on the top.The DI water droplets are also demonstrated to be transported in two dimensions smoothly in a programmable manner,and the maximum transport speed reaches 96 mm/s.The droplets of normal saline,a solution of 0.9 wt%NaCl,are also successfully manipulated on the chip.展开更多
基金This work was partly supported by grants from the National Key Research and Development Program of China(Grant No.:2017YFA0104500)from the Ministry of Science and TechnologyNational Natural Science Foundation of China(Grant No.:81770189,81621001,and 81530046)+3 种基金the Science and Technology Project of Guangdong Province of China(Grant No.:2016B030230003)Peking University Clinical Scientist Program(Grant No.:BMU2019LCKXJ003)the Fundamental Research Funds for the Central Universitiesthe project of health collaborative innovation of Guangzhou city(Grant No.:201704020214).
文摘Background:Human leukocyte antigen-identical sibling donor(ISD)-hematopoietic stem cell transplantation(SCT)is a potentially curative treatment for high-risk pediatric acute myeloid leukemia(AML).A haploidentical donor(HID)is readily available to almost all children.Previous studies have demonstrated that patients with HID-SCT had similar outcomes compared to ISD-SCT for pediatric and adult AML.However,the role of HID-SCT in high-risk pediatric AML is unclear.Methods:To compare the overall survival of high-risk AML children who underwent either HID-SCT or ISD-SCT,we analyzed 179 cases of high-risk AML patients under 18 years of age treated with either ISD-SCT(n=23)or HID-SCT(n=156).Granulocyte colony-stimulating factor plus anti-thymocyte globulin-based regimens were used for HID-SCT.We also analyzed the subgroup data of AML patients at first complete remission(CR1)before SCT with known cytogenetic risk.Results:The numbers of adverse cytogenetic risk recipients were 8(34.8%)and 13(18.8%)in the ISD-SCT group and the HID-SCT group,and the number of patients with disease status beyond CR1 were 6(26.1%)and 14(20.3%)in the two groups.The cumulative rates of grades II-IV acute graft-versus-host disease(GVHD)were 13.0%in the ISD-SCT group and 34.8%in the HID-SCT group(P=0.062),with a three-year cumulative rates of chronic GVHD at 14.1%and 34.9%,respectively(P=0.091).The relapse rate in the ISD-SCT group was significantly higher than that in the HID-SCT group(39.1%vs.16.4%,P=0.027);with non-relapse mortality at 0.0%and 10.6%(P=0.113),respectively.The three-year overall survival rates were 73.0%for the ISD-SCT group and 74.6%for the HID-SCT group(P=0.689).In subgroup analysis,the three-year relapse rate in the ISD-SCT group was higher than that in the HID-SCT group(50.0%vs.9.2%,P=0.001)and the three-year DFS in the ISD-SCT group(50.0%)was lower than that in the HID-SCT group(81.2%)(P=0.021).Conclusions:Unmanipulated HID-SCT achieved DFS and OS outcomes comparable to those of ISD-SCT for high-risk pediatric AML patients wi
文摘基于溶血曼氏杆菌(Mannheimia haemolytica,M.haemolytica)单拷贝基因sodA,通过优化反应条件和体系,建立了溶血曼氏杆菌ddPCR(droplet digital polymerase chain reaction,ddPCR)定量检测方法。结果显示,所建立的溶血曼氏杆菌ddPCR方法特异性好;检出限(limit of detection,LOD)和定量值(limit of quantification,LOQ)均为170 CFU/m L,3个平行检测的变异系数均小于25%,重复性良好。针对系列稀释的溶血曼氏杆菌纯菌液,ddPCR法能够实现对溶血曼氏杆菌的准确定量,与平板计数结果偏差小于4%。运用所建立的ddPCR方法对15份牛鼻拭子样品进行检测,结果在10份样品中检出溶血曼氏杆菌,含量在3.07×10^(2)~9.52×10^(4)CFU/mL之间。本研究所建立的ddPCR方法适用于临床鼻拭子样品中溶血曼氏杆菌的精准定量分析,可为牛群中溶血曼氏杆菌的监测和治疗提供有效的技术支持。
基金Acknowledgements： This work was supported by the Foundations of Post Doctor of China （No. 20060401001） and by the Science Research Projects of Ministry of Education of China （No. 06JA630056） and by the Natural Science Foundations of Ningxia （No. NZ0848）.
基金by the National Basic Research Program of China under Grant No 2011CB309501the Fund for Creative Research of NSFC under Grant No 61021064+1 种基金the Key Project of NSFC under Grant No 60936001the National Natural Science Foundation of China under Grant No 60876037.
文摘A low-voltage-driven digital-droplet-transporting chip with an open structure is designed,fabricated and characterized.The digital microfluidic chip is fabricated by the silicon planar process.Using only a single electrode panel,the droplet on the chip can be manipulated by electrostatic force under a dc driving voltage.The actuation principle is proposed and verified by the experiment.The experimental results show that the minimum driving voltage decreases as the thickness of the dielectric layer decreases.The driving voltage for a 3µL deionized(DI)water droplet is reduced to 15 V in air and 13.5 V in oil by employing a thin dielectric layer of 600 nm with a high dielectric constant and a coating hydrophobic layer on the top.The DI water droplets are also demonstrated to be transported in two dimensions smoothly in a programmable manner,and the maximum transport speed reaches 96 mm/s.The droplets of normal saline,a solution of 0.9 wt%NaCl,are also successfully manipulated on the chip.
