Vascular endothelial growth factor(VEGF)was discovered by its angiogenic activity.However,during evolution,it appeared earlier as a neurotrophic factor required for the development of the nervous system in invertebrat...Vascular endothelial growth factor(VEGF)was discovered by its angiogenic activity.However,during evolution,it appeared earlier as a neurotrophic factor required for the development of the nervous system in invertebrates lacking a circulatory system.We aimed at reviewing recent evidence indicating that VEGF has neuroprotective effects in neurons exposed to a variety of insults.Of particular interest is the link established between VEGF and motoneurons,especially after the design of the VEGFδ/δmutant mice.These mice are characterized by low levels of VEGF and develop muscle weakness and motoneuron degeneration resembling amyotrophic lateral sclerosis.The administration of VEGF through several routes to animal models of amyotrophic lateral sclerosis delays motor impairment and motoneuron degeneration and increases life expectancy.There are new recent advances in the role of VEGF in the physiology of motoneurons.Our experimental aims use the extraocular(abducens)motoneurons lesioned by axotomy as a model for studying VEGF actions.Axotomized abducens motoneurons exhibit severe alterations in their discharge activity and a loss of synaptic boutons.The exogenous administration of VEGF to axotomized abducens motoneurons,either from the transected nerve or intraventricularly,fully restores the synaptic and discharge properties of abducens motoneurons,despite being axotomized.In addition,when an anti-VEGF neutralizing antibody is delivered from the muscle to intact,uninjured abducens motoneurons,these cells display alterations in their discharge pattern and a loss of synaptic boutons that resemble the state of axotomy.All these data indicate that VEGF is an essential neurotrophic factor for motoneurons.展开更多
ObjectiveTo evaluate the anti-inflammatory, analgesic, antioxidant and acute toxicity of extracts obtained from a successive extraction with solvents of ascending polarity [hexane, hex; chloroform, CHCl<sub>3<...ObjectiveTo evaluate the anti-inflammatory, analgesic, antioxidant and acute toxicity of extracts obtained from a successive extraction with solvents of ascending polarity [hexane, hex; chloroform, CHCl<sub>3</sub> and ethanol (EtOH)] of Ternstroemia sylvatica Schltdl. & Cham.MethodsThe antioxidant potential was evaluated by 2,2 diphenyl-1-picrylhydrazyl, the ferric reducing/antioxidant power assays and by determining the total phenolic content. The anti-inflammatory and antinociceptive effects were evaluated using the in vivo croton oil-induced ear edema, phorbol 12-myristate 13-acetate induced ear edema, carrageenan-induced paw edema, acetic acid-induced writhing and formalin murine models. The acute toxicity was tested using the Lorke’s method in mice.ResultsThe EtOH extract was the most active for the antioxidant potential tests diphenyl-1-picrylhydrazyl (68.70% inhibition), ferric reducing/antioxidant power [(2 431.30 ± 102.10) mmol Fe<sup>2+</sup> and total polyphenols content (215.80 ± 8.50) meqAG/g]. The anti-inflammatory activity was evaluated by topical application of croton oil (2 mg/ear dose) where the EtOH extract showed the strongest activity compared to the control group (45.13% inhibition), whereas in the phorbol 12-myristate 13-acetate model, at the same dose, the CHCl<sub>3</sub> extract showed the highest inhibition (42.88%). In the carrageenan induced edema model, the EtOH extract showed a stronger inhibition compared to indomethacin (56.34% and 50.70% at doses of 250 and 500 mg/kg of extract, respectively) during the first hour. Similarly, the same extract showed the highest analgesic activity (30.60% inhibition) in the acetic acid contortion assay, and in the formalin test it showed a greater effect with respect to the control group in both phases.ConclusionsOur work confirms the value of Ternstroemia sylvatica as an important anti-inflammatory and analgesic plant, whose mechanism seems to be associated to its antioxidant effects, and supports its uses in the Mexican traditional 展开更多
Hydrogen (H<sub>2</sub>) production from experiments with Spirulina maxima 2342 is reported in this work. The performance of this photosynthetic microorganism for producing H<sub>2</sub> was ev...Hydrogen (H<sub>2</sub>) production from experiments with Spirulina maxima 2342 is reported in this work. The performance of this photosynthetic microorganism for producing H<sub>2</sub> was evaluated for the first time under specific experimental conditions (e.g., a biomass concentration of 0.34 ± 0.02 g, a light intensity of 150 μE.s<sup>-1</sup>.m<sup>-2</sup> and reaction times of 19.3 ± 1.2 h). The performance of this photosynthetic microorganism for producing hydrogen was successfully improved by the addition of sodium dithionite (a reducing agent) as an innovative method for increasing the gas production, and as a main contribution of this work. Quantitative gas chromatography (GC) analyses of H<sub>2</sub> to verify the production performance were successfully carried out at low concentration levels. GC analyses were performed by means of a conventional thermal conductivity detector coupled to a separation system of a Molecular Sieve column 500 mm × 3175 mm (L × ID). Low detection limits were consistently obtained with the GC system used. The separation of H<sub>2</sub> in culture samples was efficiently achieved in average retention times of 1.47 min. The H<sub>2</sub> produced in this process was subsequently used for power generation using a Proton Exchange Membrane Fuel Cell (PEMFC).展开更多
To address current challenges regarding sustainable development of wastewater treatment and provide scientific support in decision procedures towards sustainable solutions, new approaches, frameworks and methodologies...To address current challenges regarding sustainable development of wastewater treatment and provide scientific support in decision procedures towards sustainable solutions, new approaches, frameworks and methodologies about different possible solutions and their potential sustainability implications are needed. One way to facilitate sustainability assessment of wastewater is Life Cycle Assessment (LCA) methodology;however, it fails to map the full scope of wastewater impacts. This paper presents a framework to evaluate the performance of Wastewater Treatment Facilities (WWTF) taking into consideration various factors for insuring environmental sustainability. A total of nine indicators, seven environmental and two economic related to four wastewater treatment facilities, were assessed. Apart from evaluating the sustainability, this study also discussed the link of life cycle approach and social aspects of wastewater. The results show that for the environmental dimension using LCA provides information on different types of environmental activities and different impact categories. LCA can thus be used to quantify and compare the multiple types of impacts caused by one type of use or emission, as well as the various resource uses or emissions that contribute to one type of impacts. For the economic dimension, there is still a need for consistent and robust indicators and methods. The empirical results suggest that the environmental sustainability framework can be used in the first phase of the decision procedure that leads to a strategic choice for sustainable resource recovery from wastewater in developing countries. This motives researchers and decision-makers to consider the whole picture, and not just individual aspects, when considering different futures scenarios.展开更多
基金supported by the I+D+i project P20_00529 Consejería de Transformación Económica Industria y Conocimiento,Junta de Andalucía-FEDERsupported by project PGC2018-094654-B-100 and PID2021-124300NB-I00 funded by MCIN/AEI/FEDER“A way of making Europe”+1 种基金P.M.C.was a scholar of Ministerio de Educación y Ciencia(BES-2016-077912)in Spain and is now a“Margarita Salas”postdoctoral fellowRGH is a postdoctoral fellow from PAIDI-2019,“Talento Doctores”Junta de Andalucía in Spain,and is now a“Ramón y Cajal”fellow in Spain。
文摘Vascular endothelial growth factor(VEGF)was discovered by its angiogenic activity.However,during evolution,it appeared earlier as a neurotrophic factor required for the development of the nervous system in invertebrates lacking a circulatory system.We aimed at reviewing recent evidence indicating that VEGF has neuroprotective effects in neurons exposed to a variety of insults.Of particular interest is the link established between VEGF and motoneurons,especially after the design of the VEGFδ/δmutant mice.These mice are characterized by low levels of VEGF and develop muscle weakness and motoneuron degeneration resembling amyotrophic lateral sclerosis.The administration of VEGF through several routes to animal models of amyotrophic lateral sclerosis delays motor impairment and motoneuron degeneration and increases life expectancy.There are new recent advances in the role of VEGF in the physiology of motoneurons.Our experimental aims use the extraocular(abducens)motoneurons lesioned by axotomy as a model for studying VEGF actions.Axotomized abducens motoneurons exhibit severe alterations in their discharge activity and a loss of synaptic boutons.The exogenous administration of VEGF to axotomized abducens motoneurons,either from the transected nerve or intraventricularly,fully restores the synaptic and discharge properties of abducens motoneurons,despite being axotomized.In addition,when an anti-VEGF neutralizing antibody is delivered from the muscle to intact,uninjured abducens motoneurons,these cells display alterations in their discharge pattern and a loss of synaptic boutons that resemble the state of axotomy.All these data indicate that VEGF is an essential neurotrophic factor for motoneurons.
文摘ObjectiveTo evaluate the anti-inflammatory, analgesic, antioxidant and acute toxicity of extracts obtained from a successive extraction with solvents of ascending polarity [hexane, hex; chloroform, CHCl<sub>3</sub> and ethanol (EtOH)] of Ternstroemia sylvatica Schltdl. & Cham.MethodsThe antioxidant potential was evaluated by 2,2 diphenyl-1-picrylhydrazyl, the ferric reducing/antioxidant power assays and by determining the total phenolic content. The anti-inflammatory and antinociceptive effects were evaluated using the in vivo croton oil-induced ear edema, phorbol 12-myristate 13-acetate induced ear edema, carrageenan-induced paw edema, acetic acid-induced writhing and formalin murine models. The acute toxicity was tested using the Lorke’s method in mice.ResultsThe EtOH extract was the most active for the antioxidant potential tests diphenyl-1-picrylhydrazyl (68.70% inhibition), ferric reducing/antioxidant power [(2 431.30 ± 102.10) mmol Fe<sup>2+</sup> and total polyphenols content (215.80 ± 8.50) meqAG/g]. The anti-inflammatory activity was evaluated by topical application of croton oil (2 mg/ear dose) where the EtOH extract showed the strongest activity compared to the control group (45.13% inhibition), whereas in the phorbol 12-myristate 13-acetate model, at the same dose, the CHCl<sub>3</sub> extract showed the highest inhibition (42.88%). In the carrageenan induced edema model, the EtOH extract showed a stronger inhibition compared to indomethacin (56.34% and 50.70% at doses of 250 and 500 mg/kg of extract, respectively) during the first hour. Similarly, the same extract showed the highest analgesic activity (30.60% inhibition) in the acetic acid contortion assay, and in the formalin test it showed a greater effect with respect to the control group in both phases.ConclusionsOur work confirms the value of Ternstroemia sylvatica as an important anti-inflammatory and analgesic plant, whose mechanism seems to be associated to its antioxidant effects, and supports its uses in the Mexican traditional
文摘Hydrogen (H<sub>2</sub>) production from experiments with Spirulina maxima 2342 is reported in this work. The performance of this photosynthetic microorganism for producing H<sub>2</sub> was evaluated for the first time under specific experimental conditions (e.g., a biomass concentration of 0.34 ± 0.02 g, a light intensity of 150 μE.s<sup>-1</sup>.m<sup>-2</sup> and reaction times of 19.3 ± 1.2 h). The performance of this photosynthetic microorganism for producing hydrogen was successfully improved by the addition of sodium dithionite (a reducing agent) as an innovative method for increasing the gas production, and as a main contribution of this work. Quantitative gas chromatography (GC) analyses of H<sub>2</sub> to verify the production performance were successfully carried out at low concentration levels. GC analyses were performed by means of a conventional thermal conductivity detector coupled to a separation system of a Molecular Sieve column 500 mm × 3175 mm (L × ID). Low detection limits were consistently obtained with the GC system used. The separation of H<sub>2</sub> in culture samples was efficiently achieved in average retention times of 1.47 min. The H<sub>2</sub> produced in this process was subsequently used for power generation using a Proton Exchange Membrane Fuel Cell (PEMFC).
文摘To address current challenges regarding sustainable development of wastewater treatment and provide scientific support in decision procedures towards sustainable solutions, new approaches, frameworks and methodologies about different possible solutions and their potential sustainability implications are needed. One way to facilitate sustainability assessment of wastewater is Life Cycle Assessment (LCA) methodology;however, it fails to map the full scope of wastewater impacts. This paper presents a framework to evaluate the performance of Wastewater Treatment Facilities (WWTF) taking into consideration various factors for insuring environmental sustainability. A total of nine indicators, seven environmental and two economic related to four wastewater treatment facilities, were assessed. Apart from evaluating the sustainability, this study also discussed the link of life cycle approach and social aspects of wastewater. The results show that for the environmental dimension using LCA provides information on different types of environmental activities and different impact categories. LCA can thus be used to quantify and compare the multiple types of impacts caused by one type of use or emission, as well as the various resource uses or emissions that contribute to one type of impacts. For the economic dimension, there is still a need for consistent and robust indicators and methods. The empirical results suggest that the environmental sustainability framework can be used in the first phase of the decision procedure that leads to a strategic choice for sustainable resource recovery from wastewater in developing countries. This motives researchers and decision-makers to consider the whole picture, and not just individual aspects, when considering different futures scenarios.
