As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, envir...As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, environmental pollution, and ultimately human health, timely and higher resolution land cover information is urgently needed to achieve the sustainable development goals of the United Nations.展开更多
AIM:To investigate the correlation between mitogen-activated protein kinase (MAPK) signal transduction pathway and multidrug resistance (MDR) in MGC803 cells.METHODS:Western blot was used to analyze the expression of ...AIM:To investigate the correlation between mitogen-activated protein kinase (MAPK) signal transduction pathway and multidrug resistance (MDR) in MGC803 cells.METHODS:Western blot was used to analyze the expression of MDR associated gene in transient vincristine (VCR) induced MGC803 cells, which were treated with or without the specific inhibitor of MAPK, PD098059.Morphologic analysis of the cells treated by VCR with or without PD098059 was determined by Wright-Giemsa staining. The cell cycle analysis was performed by using flow cytometric assay and the drug sensitivity of MGC803 cells which were exposed to VCR with or without PD098059 was tested by using MTT assay.RESULTS:Transient exposure to VCR induced P-gp butnot MRP1 or GST-π expression in MGC803 cells and the expression of P-gp was inhibited by PD098059.Apoptotic bodies were found in the cells treated with VCR or VCR+PD098059. FCM results indicated that more MGC803 cells showed apoptotic phenotype when treated by VCR and PD098059 (rate:31.23%) than treated by VCR only (rate:18.42%) (P<0.05).The IC50(284±13.2 μg/L) of MGC803 cells pretreated with VCR was 2.24-fold as that of negative control group (127±17.6μg/L) and 1.48-fold as that of the group treated with PD098059 (191±27.9μg/L).CONCLUSION:This study shows that the expression of P-gp can be induced by transient exposure to VCR and this induction can be prevented by PD098059, which can block the activity of MAPK. MAPK signal transduction pathway may play some roles in modulating MDR1 expression in gastric cancer.展开更多
Inflammation is indispensable for host defense,whereas excessive inflammation often develop inflammatory diseases.Autophagy is thought to be engaged in many extracellular stress responses,such as starvation and innate...Inflammation is indispensable for host defense,whereas excessive inflammation often develop inflammatory diseases.Autophagy is thought to be engaged in many extracellular stress responses,such as starvation and innate immunity.Thus,autophagy plays an important role in maintaining homeostasis.The purpose of this study was to elucidate the function of BRF1 in the regulation of inflammation and autophagy response in macrophages.We found that BRF1 inhibited the LPS-induced inflammatory factors expression and the autophagy flux in macrophage.Furthermore,inhibition autophagy with 3-MA can attenuate the suppressive effect of BRF1 on LPS-mediated inflammation.In addition,MAPK/ERK signaling pathway was involved in the BRF1 inhibition inflammation and autophagy in macrophages.These findings indicate that BRF1 attenuates LPS-induced inflammatory factors secretion through autophagy,at least in part,through MAPK/ERK signaling pathway.展开更多
The necessity of disease models for bone/cartilage related disorders is well-recognized,but the barrier between ex-vivo cell culture,animal models and the real human body has been pending for decades.The organoid-on-a...The necessity of disease models for bone/cartilage related disorders is well-recognized,but the barrier between ex-vivo cell culture,animal models and the real human body has been pending for decades.The organoid-on-a-chip technique showed opportunity to revolutionize basic research and drug screening for diseases like osteoporosis and arthritis.The bone/cartilage organoid on-chip(BCoC)system is a novel platform of multi-tissue which faithfully emulate the essential elements,biologic functions and pathophysiological response under real circumstances.In this review,we propose the concept of BCoC platform,summarize the basic modules and current efforts to orchestrate them on a single microfluidic system.Current disease models,unsolved problems and future challenging are also discussed,the aim should be a deeper understanding of diseases,and ultimate realization of generic ex-vivo tools for further therapeutic strategies of pathological conditions.展开更多
Based on the sand dust storms data and climatic data in 12 meteorological stations around sand dust storm originating areas of the Taklimakan Desert, we analyzed the trends of the number of dust storm days from 1960 t...Based on the sand dust storms data and climatic data in 12 meteorological stations around sand dust storm originating areas of the Taklimakan Desert, we analyzed the trends of the number of dust storm days from 1960 to 2005 as well as their correlations with temperature, precipitation, wind speed and the number of days with mean wind speed 〉 5 m/s. The results show that the frequency of dust storm events in the Taklimakan region decreased with the elapse of time. Except Ruoqiang and Minfeng, in the other 10 meteorological stations, the frequency of dust storm events reduces, and in 4 meteorological stations of Kuqa, Korla, Kalpin and Hotan, the frequency of dust storm events distinctly decreases. The temperature has an increasing trend, while the average wind speed and the number of days with mean wind speed ≥ 5 m/s have decreasing trends. The correlation analysis between the number of days of dust storms and climatic parameters demonstrates that wind speed and the number of days with mean wind speed 〉 5 m/s have strong positive correlation with the number of days of dust storms, with the correlations coefficients being 0.743 and 0.720 （p〈0.01）, respectively, which indicates that strong wind is the direct factor resulting in sand dust storms. Whereas precipitation has significant negative correlation with the number of days of dust storms （p〈0.01）, and the prior annual precipitation has also negative correlation, which indicates that the prior precipitation restrains the occurrence of sand dust storms, but this restraining action is weaker than the same year＇s precipitation. Temperature has negative correlation with the number of dust storm days, with a correlations coefficient of -0.433 （p〈0.01）, which means that temperature change also has impacts on the occurrence of dust storm events in the Taklimakan region.展开更多
Carbon nanofibers with hollow structures have an excellent application prospect in various fields be-cause of their high specific surface area and abundant active sites.PAN-based hollow carbon nanofiber doped with Co/...Carbon nanofibers with hollow structures have an excellent application prospect in various fields be-cause of their high specific surface area and abundant active sites.PAN-based hollow carbon nanofiber doped with Co/CoO(H-Co/CoO-CNF)was successfully prepared and used as a catalyst to activate perox-ymonosulfate(PMS)and degrade Rhodamine B(RhB).The catalyst showed a surprising degradation rate(98.89%)of RhB within 15 min and had good degradation performance in a wide pH range(pH 1.5-11.2).Compared with solid fibers,H-Co/CoO-CNF shows better cyclic characteristics.The catalyst is also mag-netic and recoverable easily due to the addition of Co/CoO.Two pathways of both radical(SO_(4)·^(−))and non-radical(^(1)O_(2))exist during the RhB degradation process are confirmed through electron paramagnetic resonance(EPR)analysis and radical quenching experiments.This work provides a new idea for hollow fibers loaded with metals and their oxides and can guide the development of catalysts for advanced oxi-dation processes in the future.展开更多
Rapid and effective repair of injured or diseased bone defects remains a major challenge due to shortages of implants.Smart hydrogels that respond to internal and external stimuli to achieve therapeutic actions in a s...Rapid and effective repair of injured or diseased bone defects remains a major challenge due to shortages of implants.Smart hydrogels that respond to internal and external stimuli to achieve therapeutic actions in a spatially and temporally controlled manner have recently attracted much attention for bone therapy and regeneration.These hydrogels can be modified by introducing responsive moieties or embedding nanoparticles to increase their capacity for bone repair.Under specific stimuli,smart hydrogels can achieve variable,programmable,and controllable changes on demand to modulate the microenvironment for promoting bone healing.In this review,we highlight the advantages of smart hydrogels and summarize their materials,gelation methods,and properties.Then,we overview the recent advances in developing hydrogels that respond to biochemical signals,electromagnetic energy,and physical stimuli,including single,dual,and multiple types of stimuli,to enable physiological and pathological bone repair by modulating the microenvironment.Then,we discuss the current challenges and future perspectives regarding the clinical translation of smart hydrogels.展开更多
OBJECTIVE:To investigate the effect of Taohong Siwu decoction(桃红四物汤,TSD)on atherosclerosis in rats as well as investigate the underlying mechanism based on molecular docking.METHODS:Sixty healthy male Sprague-Daw...OBJECTIVE:To investigate the effect of Taohong Siwu decoction(桃红四物汤,TSD)on atherosclerosis in rats as well as investigate the underlying mechanism based on molecular docking.METHODS:Sixty healthy male Sprague-Dawley rats were randomly divided into 6 groups with 10 rats in each group:control group,model group,atorvastatin group(AT,2.0 mg/kg),and TSD groups(20,10,5 g/kg)after 7 d of acclimation.The model of atherosclerosis was successfully established except the control group by high fat diet(HFD)and vitamin D2.Biochemical analyzers were used to detect the levels of triglyceride(TG),total cholestero(TC),low density lipoprotein-cholesterol(LDLC)and high density lipid-cholesterol(HDL-C)in blood lipid.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)were determined by enzyme-linked immunosorbent assay.Sudan IV staining and Hematoxylin and eosin staining(HE staining)were performed to observe the pathological changes in aortic tissue.Molecular docking technology was used to predict the best matching between the main components of TSD and the target proteins.The expression of target proteins was further detected by quantitative real time polymerase chain reaction(q RTPCR)and Western blot analysis.RESULTS:The results showed that TSD restricted atherosclerosis development and decreased the inflammatory cytokines in plasma.Molecular docking results predicted that the main components of TSD showed a strong binding ability with toll-like receptor(TLR4),myeloid differentiation primary response protein 88(My D88),and nuclear factor kappa-B(NF-κB).The results of q RT-PCR and Western blot analysis showed that the m RNA and protein expressions of TLR4,My D88 and NF-κB p65 in the aorta were reduced in atorvastatin group and TSD group.CONCLUSIONS:TSD can ameliorate atherosclerosis in rats,and the underlying mechanism is supposed be related to the suppression of inflammatory response by regulating TLR4/My D88/NF-κB signal pathway.展开更多
Vehicles operating in space need to withstand extreme thermal and electromagnetic environments in light of the burgeoning of space science and technology.It is imperatively desired to high insulation materials with li...Vehicles operating in space need to withstand extreme thermal and electromagnetic environments in light of the burgeoning of space science and technology.It is imperatively desired to high insulation materials with lightweight and extensive mechanical properties.Herein,a boron-silica-tantalum ternary hybrid phenolic aerogel(BSiTa-PA)with exceptional thermal stability,extensive mechanical strength,low thermal conductivity(49.6 mW m^(-1)K^(-1)),and heightened ablative resistance is prepared by an expeditious method.After extremely thermal erosion,the obtained carbon aerogel demonstrates noteworthy electromagnetic interference(EMI)shielding performance with an efficiency of 31.6 dB,accompanied by notable loading property with specific modulus of 272.8 kN·m kg^(-1).This novel design concept has laid the foundation for the development of insulation materials in more complex extreme environments.展开更多
Cartilage development is controlled by the highly synergistic proliferation and differentiation of growth plate chondrocytes,in which the Indian hedgehog(IHH)and parathyroid hormone-related protein-parathyroid hormone...Cartilage development is controlled by the highly synergistic proliferation and differentiation of growth plate chondrocytes,in which the Indian hedgehog(IHH)and parathyroid hormone-related protein-parathyroid hormone-1 receptor(PTHrP-PTH1R)feedback loop is crucial.The inositol-requiring enzyme 1a/X-box-binding protein-1 spliced(IRE1α/XBP1s)branch of the unfolded protein response(UPR)is essential for normal cartilage development.However,the precise role of ER stress effector IRE1α,encoded by endoplasmic reticulum to nucleus signaling 1(ERN1),in skeletal development remains unknown.Herein,we reported that loss of IRE1α accelerates chondrocyte hypertrophy and promotes endochondral bone growth.ERN1 acts as a negative regulator of chondrocyte proliferation and differentiation in postnatal growth plates.Its deficiency interrupted PTHrP/PTH1R and IHH homeostasis leading to impaired chondrocyte hypertrophy and differentiation.XBP1s,produced by p-IRE1α-mediated splicing,binds and up-regulates PTH1R and IHH,which coordinate cartilage development.Meanwhile,ER stress cannot be activated normally in ERN1-deficient chondrocytes.In conclusion,ERN1 deficiency accelerates chondrocyte hypertrophy and cartilage mineralization by impairing the homeostasis of the IHH and PTHrP/PTH1R feedback loop and ER stress.ERN1 may have a potential role as a new target for cartilage growth and maturation.展开更多
Diabetes mellitus is a chronically inflamed disease that predisposes to delayed fracture healing.Macrophages play a key role in the process of fracture healing by undergoing polarization into either M1 or M2 subtypes,...Diabetes mellitus is a chronically inflamed disease that predisposes to delayed fracture healing.Macrophages play a key role in the process of fracture healing by undergoing polarization into either M1 or M2 subtypes,which respectively exhibit pro-inflammatory or anti-inflammatory functions.Therefore,modulation of macrophage polarization to the M2 subtype is beneficial for fracture healing.Exosomes perform an important role in improving the osteoimmune microenvironment due to their extremely low immunogenicity and high bioactivity.In this study,we extracted the M2-exosomes and used them to intervene the bone repair in diabetic fractures.The results showed that M2-exosomes significantly modulate the osteoimmune microenvironment by decreasing the proportion of M1 macrophages,thereby accelerating diabetic fracture healing.We further confirmed that M2-exosomes induced the conversion of M1 macrophages into M2 macrophages by stimulating the PI3K/AKT pathway.Our study offers a fresh perspective and a potential therapeutic approach for M2-exosomes to improve diabetic fracture healing.展开更多
Tropical cyclone,a high energy destructive meteorological system with heavy rainfall and gale triggered massive landslides and windstorms,poses a significant threat to coastal areas.In this paper we have developed a T...Tropical cyclone,a high energy destructive meteorological system with heavy rainfall and gale triggered massive landslides and windstorms,poses a significant threat to coastal areas.In this paper we have developed a Tropical Cyclone Potential Impact Index （TCPI） based on the air mass trajectories,disaster information,intensity,duration,and frequency of tropical cyclones.We analyzed the spatial pattern and interannual variation of the TCPI over the period 1949-2009,and taking the Super Typhoon Saomai as an example have examined the relationship between the TCPI and direct economic losses,total rainfall,and maximum wind speed.The results reveal that China＇s TCPI appears to be a weak decreasing trend over the period,which is not significant overall,but significant in some periods.Over the past 20 years,the TCPI decreased in the southern China coastal provinces of Hainan,Guangdong and Guangxi,while it increased in the southeastern coastal provinces of Zhejiang,Fujian and Taiwan.The highest values of TCPI are mainly observed in Taiwan,Hainan,the coastal areas of Guangdong and Fujian and Zhejiang＇s southern coast.The TCPI has a good correlation （P=0.01） with direct economic loss,rainfall,and maximum wind speed.展开更多
Progranulin(PGRN)is a multifunctional growth factor involved in many physiolog-ical processes and disease states.The apparent protective role of PGRN and the importance of chondrocyte autophagic function in the progre...Progranulin(PGRN)is a multifunctional growth factor involved in many physiolog-ical processes and disease states.The apparent protective role of PGRN and the importance of chondrocyte autophagic function in the progression of osteoarthritis(OA)led us to investi-gate the role of PGRN in the regulation of chondrocyte autophagy.PGRN knockout chondro-cytes exhibited a deficient autophagic response with limited induction following rapamycin,serum starvation,and IL-1b-induced autophagy.PGRN-mediated anabolism and suppression of IL-1b-induced catabolism were largely abrogated in the presence of the BafA1 autophagy inhibitor.Mechanistically,during the process of OA,PGRN and the ATG5eATG12 conjugate form a protein complex;PGRN regulates autophagy in chondrocytes and OA through,at least partially,the interactions between PGRN and the ATG5eATG12 conjugate.Furthermore,the ATG5eATG12 conjugate is critical for cell proliferation and apoptosis.Knockdown or knockout of ATG5 reduces the expression of ATG5eATG12 conjugate and inhibits the chondroprotective effect of PGRN on anabolism and catabolism.Overexpression of PGRN partially reversed this effect.In brief,the PGRN-mediated regulation of chondrocyte autophagy plays a key role in the chondroprotective role of PGRN in OA.Such studies provide new insights into the pathogen-esis of OA and PGRN-associated autophagy in chondrocyte homeostasis.展开更多
Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA i...Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.展开更多
In this study, chemical reduced graphene-silver nanoparticles hybrid （AgNPs@CR-GO） with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between im- ...In this study, chemical reduced graphene-silver nanoparticles hybrid （AgNPs@CR-GO） with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between im- mobilized enzyme and electrode. A facile route to prepare AgNPs@CR-GO was designed involving in β-cyclodextrin （β-CD） as reducing and stabilizing agent. The morphologies of AgNPs were regulated and controlled by various experimental factors. To fabricate the bioelectrode, AgNPs@CR-GO was modified on glassy carbon electrode followed by immobilization of glucose oxidase （GOx） or laccase. It was demonstrated by electrochemical testing that the electrode with close-packed AgNPs provided high GOx loading （Г=4.80 × 10^- l0 mol·cm^-2） and fast electron transfer rate （ks=5.76 s^-1）. By employing GOx based-electrode as anode and laccase based-electrode as cathode, the assembled enzymatic biofuel cell exhibited a maximum power density of 77.437 μW·cm^-2 and an open-circuit voltage of 0.705 V.展开更多
Objective： To investigate the expression and significance of cancer inhibitory gene PTEN protein in osteosar-coma. To analyze the level of its expression in different histological classification of osteosarcoma. To d...Objective： To investigate the expression and significance of cancer inhibitory gene PTEN protein in osteosar-coma. To analyze the level of its expression in different histological classification of osteosarcoma. To determine the possibility of taking PTEN protein as a marker gene for diagnosing osteosarcoma. To observe the clinical value of PTEN expression levels as a reference index for osteosarcoma classification. Methods： 43 specimens collected from osteosarcoma excision were studied. 30 specimens collected during the same period from benign lesion of bone （osteochondroma） operation were taken as the control group. Immunohistochemistry staining （ElivisonTM two steps method） was used to detect the expression of PTEN protein in 43 cases of osteosarcoma. SPSS 10.0 was used in statistical analysis. Results： Immunohistochemistry staining showed that the positive reaction of PTEN protein was all oriented to cytoplasm, which were brown or yellowish- brown granules. By way of X^2 test, the significant difference of the positive expressions of PTEN protein between bone benign lesion and osteosarcoma （X^2 = 7.976, P 〈 0.01） was observed. Osteosarcoma with different degrees of histodifferentiation showed different level expression of PTEN protein. There was significant difference between well-differentiated osteosarcoma （grades Ⅰ-Ⅱ） and poorly-differentiated osteosarcoma （grade Ⅲ） statistically （P 〈 0.01）. The level of expression of PTEN was negatively correlated to the histological grade of osteosarcoma. There was great significance statistically （rs=-0.4922, P 〈 0.01）. Conclusion： PTEN protein may be used as candidate gene of cancer inhibitory gene： PTEN protein is a cancer suppressor gene protein which has expression in bone tumors. It might not only be used in the study of pulmonary carcinoma and neurogliocytoma, but also in the study of bone tumor; the expression of PTEN is related to benignancy or malignancy of bone tumor and their degree of differentiation. The exp展开更多
OBJECTIVE To investigate the effect of a specific inhibitor PD098059 of the extracellular-signal regulated protein kinase (ERK) pathway on the P-glycoprotein (P-gp)-mediated resistance of colon cancer cell lines SW480...OBJECTIVE To investigate the effect of a specific inhibitor PD098059 of the extracellular-signal regulated protein kinase (ERK) pathway on the P-glycoprotein (P-gp)-mediated resistance of colon cancer cell lines SW480/VCR and CoLo205NCR.METHODS SW480NCR and CoLo205NCR cells were generated byexposuring SW480 and CoLo205 cells to vincristine (VCR) (30 ng/ml) for 72h, which resulted in a comparatively higher level of P-gp expression.Western blotting was used to analyze P-gp, MRP, LRP, GST-'rr and TOPOIIexpression after exposuring the SW480 and CoLo205 cells to VCR (30 ng/ml)for 72 hrs. P-gp and pERK1/2 expressions was analyzed in SW480NCR andCoLo205/VCR cells treated with or without the specific inhibitor of MEK,PD098059. The MTT assay was used to determine the susceptibility ofSW480NCR and CoLo205NCR cells to VCR, treated with or withoutPD098059.I^F.SULI"S The results showed that VCR induced a comparatively higher levelof P-gp expression in the cell lines, but not that of MRP, LRP, GST-n- orTOPOII. P-gp expression levels were depressed significantly in SW480/VCR and COLO205/VCR cells by the specific inhibitor of MEK, PD098059.The IC50 (248 +19.6 and 215 +10.7 ng/ml) to VCR of SW480/VCR andCoLo205/VCR cells exhibited a 2.16 and 2.03-fold higher resistancecompared to the negative control group (SW480 and CoLo205 cells)(115+15.6 and 106 +11.9 ng/ml), but a 1.35 and 1.21 -fold higher resistance thanthe group treated with VCR (30 ng/ml)+ PD098059 (184 + 21.8 and 177+19.4 ng/ml).CONCLUSION This study shows that the expression of P-gp can beinduced by exposuring cells to VCR, and that this induction can be reversedby inhibiting the ERK signaling pathway at the point of MEK by its specificinhibitor, PD098059. The ERK signal-transduction pathway may play a rolein modulating mdrl expression in colon cancer.展开更多
The Yellow River Basin (YRB) is not only an important ecological barrier in north China,but also an important agricultural production base and energy base in China,playing a very important role in China’s economic an...The Yellow River Basin (YRB) is not only an important ecological barrier in north China,but also an important agricultural production base and energy base in China,playing a very important role in China’s economic and social development and ecological security.Under the combined actions of climate change and human activities,a series of ecological and environmental problems have emerged in the YRB,including degradation of glaciers and frozen soil,shortage of water resources,land desertification,aggravated soil and water loss,frequent floods and droughts,and reducing biodiversity.Warmer and wetter climate over the upper reaches and warmer and drier one in the middle and lower reaches have profoundly affected the ecological security of the YRB.In the future,the temperature in the YRB will continue to rise,extreme events will increase and the climate pattern of drought and water shortage will not be changed fundamentally,which will make the basin face more severe ecological security risks.For the current ecological problems and future ecological security risk challenges,it is necessary and urgent to take adaptive measures to deal with climate change and protect the ecological environment.The measures mainly include:strengthening the scientific research on the impact of climate change and extreme events on the ecological environment of the YRB and improving the ability of climate change risk management;strengthening the water conservation and protection in the upper reaches,the management of desert and soil erosion in the middle reaches and the ecological protection of water resources and wetlands in the lower reaches in the YRB and;collaborating with multiple departments to jointly tackle the ecological security challenges brought by climate change.展开更多
Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the...Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both mac-roscopic and microscopic observation with HE staining. Results: The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had oc-curred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia. Conclusion: A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.展开更多
基金partially supported by the National Key Research and Development Program of China(2016YFA0600103)Delos Living LLCthe Cyrus Tang Foundation
文摘As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, environmental pollution, and ultimately human health, timely and higher resolution land cover information is urgently needed to achieve the sustainable development goals of the United Nations.
文摘AIM:To investigate the correlation between mitogen-activated protein kinase (MAPK) signal transduction pathway and multidrug resistance (MDR) in MGC803 cells.METHODS:Western blot was used to analyze the expression of MDR associated gene in transient vincristine (VCR) induced MGC803 cells, which were treated with or without the specific inhibitor of MAPK, PD098059.Morphologic analysis of the cells treated by VCR with or without PD098059 was determined by Wright-Giemsa staining. The cell cycle analysis was performed by using flow cytometric assay and the drug sensitivity of MGC803 cells which were exposed to VCR with or without PD098059 was tested by using MTT assay.RESULTS:Transient exposure to VCR induced P-gp butnot MRP1 or GST-π expression in MGC803 cells and the expression of P-gp was inhibited by PD098059.Apoptotic bodies were found in the cells treated with VCR or VCR+PD098059. FCM results indicated that more MGC803 cells showed apoptotic phenotype when treated by VCR and PD098059 (rate:31.23%) than treated by VCR only (rate:18.42%) (P<0.05).The IC50(284±13.2 μg/L) of MGC803 cells pretreated with VCR was 2.24-fold as that of negative control group (127±17.6μg/L) and 1.48-fold as that of the group treated with PD098059 (191±27.9μg/L).CONCLUSION:This study shows that the expression of P-gp can be induced by transient exposure to VCR and this induction can be prevented by PD098059, which can block the activity of MAPK. MAPK signal transduction pathway may play some roles in modulating MDR1 expression in gastric cancer.
基金supported by the National Natural Science Foundation of China(81672209).
文摘Inflammation is indispensable for host defense,whereas excessive inflammation often develop inflammatory diseases.Autophagy is thought to be engaged in many extracellular stress responses,such as starvation and innate immunity.Thus,autophagy plays an important role in maintaining homeostasis.The purpose of this study was to elucidate the function of BRF1 in the regulation of inflammation and autophagy response in macrophages.We found that BRF1 inhibited the LPS-induced inflammatory factors expression and the autophagy flux in macrophage.Furthermore,inhibition autophagy with 3-MA can attenuate the suppressive effect of BRF1 on LPS-mediated inflammation.In addition,MAPK/ERK signaling pathway was involved in the BRF1 inhibition inflammation and autophagy in macrophages.These findings indicate that BRF1 attenuates LPS-induced inflammatory factors secretion through autophagy,at least in part,through MAPK/ERK signaling pathway.
基金This work was supported by grants from National Natural Science Foundation of China(No.82230071,92249303 and 82172098 to J.Su)Shanghai Committee of Science and Technology(Laboratory Animal Research Project to J.Su)+1 种基金Shanghai Baoshan District Medical Health Project(No.21-E-14 to L.Cao)China Postdoctoral Science Foundation(No.2022M722033 to Y.Hu).
文摘The necessity of disease models for bone/cartilage related disorders is well-recognized,but the barrier between ex-vivo cell culture,animal models and the real human body has been pending for decades.The organoid-on-a-chip technique showed opportunity to revolutionize basic research and drug screening for diseases like osteoporosis and arthritis.The bone/cartilage organoid on-chip(BCoC)system is a novel platform of multi-tissue which faithfully emulate the essential elements,biologic functions and pathophysiological response under real circumstances.In this review,we propose the concept of BCoC platform,summarize the basic modules and current efforts to orchestrate them on a single microfluidic system.Current disease models,unsolved problems and future challenging are also discussed,the aim should be a deeper understanding of diseases,and ultimate realization of generic ex-vivo tools for further therapeutic strategies of pathological conditions.
基金National Science and Technology support Project of the Extreme Meteorological Disasters Risk Regionalization and Impact ssessment,No.2007BAC29B05CMA project of Meteorological Disaster Assessment,No.20082012208
文摘Based on the sand dust storms data and climatic data in 12 meteorological stations around sand dust storm originating areas of the Taklimakan Desert, we analyzed the trends of the number of dust storm days from 1960 to 2005 as well as their correlations with temperature, precipitation, wind speed and the number of days with mean wind speed 〉 5 m/s. The results show that the frequency of dust storm events in the Taklimakan region decreased with the elapse of time. Except Ruoqiang and Minfeng, in the other 10 meteorological stations, the frequency of dust storm events reduces, and in 4 meteorological stations of Kuqa, Korla, Kalpin and Hotan, the frequency of dust storm events distinctly decreases. The temperature has an increasing trend, while the average wind speed and the number of days with mean wind speed ≥ 5 m/s have decreasing trends. The correlation analysis between the number of days of dust storms and climatic parameters demonstrates that wind speed and the number of days with mean wind speed 〉 5 m/s have strong positive correlation with the number of days of dust storms, with the correlations coefficients being 0.743 and 0.720 （p〈0.01）, respectively, which indicates that strong wind is the direct factor resulting in sand dust storms. Whereas precipitation has significant negative correlation with the number of days of dust storms （p〈0.01）, and the prior annual precipitation has also negative correlation, which indicates that the prior precipitation restrains the occurrence of sand dust storms, but this restraining action is weaker than the same year＇s precipitation. Temperature has negative correlation with the number of dust storm days, with a correlations coefficient of -0.433 （p〈0.01）, which means that temperature change also has impacts on the occurrence of dust storm events in the Taklimakan region.
基金financially supported by the National Natural Science Foundation of China(No.52072193)the Shandong Provin-cial Natural Science Foundation(Nos.ZR2021JQ16,ZR2019YQ19 and ZR2019BEM018)+2 种基金the Project of Shandong Province Higher Ed-ucational Science and Technology Program(No.2019KJA026)the Shandong Provincial College Students’Innovative Entrepreneurial Training(Nos.S202111065214 andS202211065062)State Key Laboratory for Modification of Chemical Fibers and Polymer Mate-rials(No.KF2217).
文摘Carbon nanofibers with hollow structures have an excellent application prospect in various fields be-cause of their high specific surface area and abundant active sites.PAN-based hollow carbon nanofiber doped with Co/CoO(H-Co/CoO-CNF)was successfully prepared and used as a catalyst to activate perox-ymonosulfate(PMS)and degrade Rhodamine B(RhB).The catalyst showed a surprising degradation rate(98.89%)of RhB within 15 min and had good degradation performance in a wide pH range(pH 1.5-11.2).Compared with solid fibers,H-Co/CoO-CNF shows better cyclic characteristics.The catalyst is also mag-netic and recoverable easily due to the addition of Co/CoO.Two pathways of both radical(SO_(4)·^(−))and non-radical(^(1)O_(2))exist during the RhB degradation process are confirmed through electron paramagnetic resonance(EPR)analysis and radical quenching experiments.This work provides a new idea for hollow fibers loaded with metals and their oxides and can guide the development of catalysts for advanced oxi-dation processes in the future.
基金This work is supported by the Natural Science Foundation of Shanghai(22ZR1424900)the Integrated Project of Major Research Plan of National Natural Science Foundation of China(92249303)the National Natural Science Foundation of China(82230071 and 82172098)。
文摘Rapid and effective repair of injured or diseased bone defects remains a major challenge due to shortages of implants.Smart hydrogels that respond to internal and external stimuli to achieve therapeutic actions in a spatially and temporally controlled manner have recently attracted much attention for bone therapy and regeneration.These hydrogels can be modified by introducing responsive moieties or embedding nanoparticles to increase their capacity for bone repair.Under specific stimuli,smart hydrogels can achieve variable,programmable,and controllable changes on demand to modulate the microenvironment for promoting bone healing.In this review,we highlight the advantages of smart hydrogels and summarize their materials,gelation methods,and properties.Then,we overview the recent advances in developing hydrogels that respond to biochemical signals,electromagnetic energy,and physical stimuli,including single,dual,and multiple types of stimuli,to enable physiological and pathological bone repair by modulating the microenvironment.Then,we discuss the current challenges and future perspectives regarding the clinical translation of smart hydrogels.
基金Supported by the National Natural Science Foundation of China:Investigating the Mechanism of Total Saponins in Treating Gouty Arthritis Based on Toll-like Receptor/Myeloid Differentiation Factor 88/Nuclear Factor-Kappa B Signal Pathway and Nacht Leucine-Rich Repeat Protein 3-Inflammasome(No.81573670)Study on the Material Basis and Pathway of Taohong Siwu Decoction in Regulating the Release of Platelet Alpha Granules in Postpartum Blood Stasis(No.81473387)+2 种基金Study on the Regulatory Mechanism of Taohong Siwu Decoction on Experimental Cerebral Ischemia Angiogenesis Based on the Messenger Effect of Platelet Microparticles(No.81503291)Investigating the Material Basis and Molecular Mechanism of Taohong Siwu Decoction Against Vascular Dementia Based on Microdialysis Technology and NOD-Like Receptor Protein 3 Inflammasome Vascular Endothelial Cell Interaction(No.81903953)the Anhui Province Key Research and Development Program:Research on the Development and Preparation of Taohong Siwu Granules(No.1704a0802141)。
文摘OBJECTIVE:To investigate the effect of Taohong Siwu decoction(桃红四物汤,TSD)on atherosclerosis in rats as well as investigate the underlying mechanism based on molecular docking.METHODS:Sixty healthy male Sprague-Dawley rats were randomly divided into 6 groups with 10 rats in each group:control group,model group,atorvastatin group(AT,2.0 mg/kg),and TSD groups(20,10,5 g/kg)after 7 d of acclimation.The model of atherosclerosis was successfully established except the control group by high fat diet(HFD)and vitamin D2.Biochemical analyzers were used to detect the levels of triglyceride(TG),total cholestero(TC),low density lipoprotein-cholesterol(LDLC)and high density lipid-cholesterol(HDL-C)in blood lipid.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)were determined by enzyme-linked immunosorbent assay.Sudan IV staining and Hematoxylin and eosin staining(HE staining)were performed to observe the pathological changes in aortic tissue.Molecular docking technology was used to predict the best matching between the main components of TSD and the target proteins.The expression of target proteins was further detected by quantitative real time polymerase chain reaction(q RTPCR)and Western blot analysis.RESULTS:The results showed that TSD restricted atherosclerosis development and decreased the inflammatory cytokines in plasma.Molecular docking results predicted that the main components of TSD showed a strong binding ability with toll-like receptor(TLR4),myeloid differentiation primary response protein 88(My D88),and nuclear factor kappa-B(NF-κB).The results of q RT-PCR and Western blot analysis showed that the m RNA and protein expressions of TLR4,My D88 and NF-κB p65 in the aorta were reduced in atorvastatin group and TSD group.CONCLUSIONS:TSD can ameliorate atherosclerosis in rats,and the underlying mechanism is supposed be related to the suppression of inflammatory response by regulating TLR4/My D88/NF-κB signal pathway.
基金the support from the Joint Fund of Advanced Aerospace Manufacturing Technology Research of National Natural Science Foundation of China(U1837601)National Natural Science Foundation of China(52273255)+3 种基金NASF Joint Fund of National Natural Science Foundation of China and China Academy of Engineering Physics(U2130118)China Postdoctoral Science Foundation(2023M732029)Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University(CX2023092)Undergraduate Innovation&Business Program in Northwestern Polytechnical University(XN2022226)。
文摘Vehicles operating in space need to withstand extreme thermal and electromagnetic environments in light of the burgeoning of space science and technology.It is imperatively desired to high insulation materials with lightweight and extensive mechanical properties.Herein,a boron-silica-tantalum ternary hybrid phenolic aerogel(BSiTa-PA)with exceptional thermal stability,extensive mechanical strength,low thermal conductivity(49.6 mW m^(-1)K^(-1)),and heightened ablative resistance is prepared by an expeditious method.After extremely thermal erosion,the obtained carbon aerogel demonstrates noteworthy electromagnetic interference(EMI)shielding performance with an efficiency of 31.6 dB,accompanied by notable loading property with specific modulus of 272.8 kN·m kg^(-1).This novel design concept has laid the foundation for the development of insulation materials in more complex extreme environments.
基金supported by the National Natural Science Foundation of China(No.81672209,81871769,82272550)the Chongqing Science and Technology Bureau(China)(No.cstc2021jcyj-bshX0214).
文摘Cartilage development is controlled by the highly synergistic proliferation and differentiation of growth plate chondrocytes,in which the Indian hedgehog(IHH)and parathyroid hormone-related protein-parathyroid hormone-1 receptor(PTHrP-PTH1R)feedback loop is crucial.The inositol-requiring enzyme 1a/X-box-binding protein-1 spliced(IRE1α/XBP1s)branch of the unfolded protein response(UPR)is essential for normal cartilage development.However,the precise role of ER stress effector IRE1α,encoded by endoplasmic reticulum to nucleus signaling 1(ERN1),in skeletal development remains unknown.Herein,we reported that loss of IRE1α accelerates chondrocyte hypertrophy and promotes endochondral bone growth.ERN1 acts as a negative regulator of chondrocyte proliferation and differentiation in postnatal growth plates.Its deficiency interrupted PTHrP/PTH1R and IHH homeostasis leading to impaired chondrocyte hypertrophy and differentiation.XBP1s,produced by p-IRE1α-mediated splicing,binds and up-regulates PTH1R and IHH,which coordinate cartilage development.Meanwhile,ER stress cannot be activated normally in ERN1-deficient chondrocytes.In conclusion,ERN1 deficiency accelerates chondrocyte hypertrophy and cartilage mineralization by impairing the homeostasis of the IHH and PTHrP/PTH1R feedback loop and ER stress.ERN1 may have a potential role as a new target for cartilage growth and maturation.
基金supported by the Integrated Project of Major Research Plan of National Natural Science Foundation of China(92249303)Key Project of the National Natural Science Foundation of China(82230071)National Natural Science Foundation of China(32101084,82202344).
文摘Diabetes mellitus is a chronically inflamed disease that predisposes to delayed fracture healing.Macrophages play a key role in the process of fracture healing by undergoing polarization into either M1 or M2 subtypes,which respectively exhibit pro-inflammatory or anti-inflammatory functions.Therefore,modulation of macrophage polarization to the M2 subtype is beneficial for fracture healing.Exosomes perform an important role in improving the osteoimmune microenvironment due to their extremely low immunogenicity and high bioactivity.In this study,we extracted the M2-exosomes and used them to intervene the bone repair in diabetic fractures.The results showed that M2-exosomes significantly modulate the osteoimmune microenvironment by decreasing the proportion of M1 macrophages,thereby accelerating diabetic fracture healing.We further confirmed that M2-exosomes induced the conversion of M1 macrophages into M2 macrophages by stimulating the PI3K/AKT pathway.Our study offers a fresh perspective and a potential therapeutic approach for M2-exosomes to improve diabetic fracture healing.
基金National Science & Technology Pillar Program during the 11th Five-Year Plan Period,No.2007BAC29B05No.2008BAK50B02
文摘Tropical cyclone,a high energy destructive meteorological system with heavy rainfall and gale triggered massive landslides and windstorms,poses a significant threat to coastal areas.In this paper we have developed a Tropical Cyclone Potential Impact Index （TCPI） based on the air mass trajectories,disaster information,intensity,duration,and frequency of tropical cyclones.We analyzed the spatial pattern and interannual variation of the TCPI over the period 1949-2009,and taking the Super Typhoon Saomai as an example have examined the relationship between the TCPI and direct economic losses,total rainfall,and maximum wind speed.The results reveal that China＇s TCPI appears to be a weak decreasing trend over the period,which is not significant overall,but significant in some periods.Over the past 20 years,the TCPI decreased in the southern China coastal provinces of Hainan,Guangdong and Guangxi,while it increased in the southeastern coastal provinces of Zhejiang,Fujian and Taiwan.The highest values of TCPI are mainly observed in Taiwan,Hainan,the coastal areas of Guangdong and Fujian and Zhejiang＇s southern coast.The TCPI has a good correlation （P=0.01） with direct economic loss,rainfall,and maximum wind speed.
基金supported by the National Natural Science Foundation of China(No.81672209)Chongqing Science and Technology Bureau(No.cstc2020jcyj-msxmX0175),ChinaChongqing Human Resources and Social Security Bureau(No.2018-389),China.
文摘Progranulin(PGRN)is a multifunctional growth factor involved in many physiolog-ical processes and disease states.The apparent protective role of PGRN and the importance of chondrocyte autophagic function in the progression of osteoarthritis(OA)led us to investi-gate the role of PGRN in the regulation of chondrocyte autophagy.PGRN knockout chondro-cytes exhibited a deficient autophagic response with limited induction following rapamycin,serum starvation,and IL-1b-induced autophagy.PGRN-mediated anabolism and suppression of IL-1b-induced catabolism were largely abrogated in the presence of the BafA1 autophagy inhibitor.Mechanistically,during the process of OA,PGRN and the ATG5eATG12 conjugate form a protein complex;PGRN regulates autophagy in chondrocytes and OA through,at least partially,the interactions between PGRN and the ATG5eATG12 conjugate.Furthermore,the ATG5eATG12 conjugate is critical for cell proliferation and apoptosis.Knockdown or knockout of ATG5 reduces the expression of ATG5eATG12 conjugate and inhibits the chondroprotective effect of PGRN on anabolism and catabolism.Overexpression of PGRN partially reversed this effect.In brief,the PGRN-mediated regulation of chondrocyte autophagy plays a key role in the chondroprotective role of PGRN in OA.Such studies provide new insights into the pathogen-esis of OA and PGRN-associated autophagy in chondrocyte homeostasis.
文摘Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.
基金This research is supported by the National Natural Science Foundation of China （Grant 51372206）.
文摘In this study, chemical reduced graphene-silver nanoparticles hybrid （AgNPs@CR-GO） with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between im- mobilized enzyme and electrode. A facile route to prepare AgNPs@CR-GO was designed involving in β-cyclodextrin （β-CD） as reducing and stabilizing agent. The morphologies of AgNPs were regulated and controlled by various experimental factors. To fabricate the bioelectrode, AgNPs@CR-GO was modified on glassy carbon electrode followed by immobilization of glucose oxidase （GOx） or laccase. It was demonstrated by electrochemical testing that the electrode with close-packed AgNPs provided high GOx loading （Г=4.80 × 10^- l0 mol·cm^-2） and fast electron transfer rate （ks=5.76 s^-1）. By employing GOx based-electrode as anode and laccase based-electrode as cathode, the assembled enzymatic biofuel cell exhibited a maximum power density of 77.437 μW·cm^-2 and an open-circuit voltage of 0.705 V.
文摘Objective： To investigate the expression and significance of cancer inhibitory gene PTEN protein in osteosar-coma. To analyze the level of its expression in different histological classification of osteosarcoma. To determine the possibility of taking PTEN protein as a marker gene for diagnosing osteosarcoma. To observe the clinical value of PTEN expression levels as a reference index for osteosarcoma classification. Methods： 43 specimens collected from osteosarcoma excision were studied. 30 specimens collected during the same period from benign lesion of bone （osteochondroma） operation were taken as the control group. Immunohistochemistry staining （ElivisonTM two steps method） was used to detect the expression of PTEN protein in 43 cases of osteosarcoma. SPSS 10.0 was used in statistical analysis. Results： Immunohistochemistry staining showed that the positive reaction of PTEN protein was all oriented to cytoplasm, which were brown or yellowish- brown granules. By way of X^2 test, the significant difference of the positive expressions of PTEN protein between bone benign lesion and osteosarcoma （X^2 = 7.976, P 〈 0.01） was observed. Osteosarcoma with different degrees of histodifferentiation showed different level expression of PTEN protein. There was significant difference between well-differentiated osteosarcoma （grades Ⅰ-Ⅱ） and poorly-differentiated osteosarcoma （grade Ⅲ） statistically （P 〈 0.01）. The level of expression of PTEN was negatively correlated to the histological grade of osteosarcoma. There was great significance statistically （rs=-0.4922, P 〈 0.01）. Conclusion： PTEN protein may be used as candidate gene of cancer inhibitory gene： PTEN protein is a cancer suppressor gene protein which has expression in bone tumors. It might not only be used in the study of pulmonary carcinoma and neurogliocytoma, but also in the study of bone tumor; the expression of PTEN is related to benignancy or malignancy of bone tumor and their degree of differentiation. The exp
文摘OBJECTIVE To investigate the effect of a specific inhibitor PD098059 of the extracellular-signal regulated protein kinase (ERK) pathway on the P-glycoprotein (P-gp)-mediated resistance of colon cancer cell lines SW480/VCR and CoLo205NCR.METHODS SW480NCR and CoLo205NCR cells were generated byexposuring SW480 and CoLo205 cells to vincristine (VCR) (30 ng/ml) for 72h, which resulted in a comparatively higher level of P-gp expression.Western blotting was used to analyze P-gp, MRP, LRP, GST-'rr and TOPOIIexpression after exposuring the SW480 and CoLo205 cells to VCR (30 ng/ml)for 72 hrs. P-gp and pERK1/2 expressions was analyzed in SW480NCR andCoLo205/VCR cells treated with or without the specific inhibitor of MEK,PD098059. The MTT assay was used to determine the susceptibility ofSW480NCR and CoLo205NCR cells to VCR, treated with or withoutPD098059.I^F.SULI"S The results showed that VCR induced a comparatively higher levelof P-gp expression in the cell lines, but not that of MRP, LRP, GST-n- orTOPOII. P-gp expression levels were depressed significantly in SW480/VCR and COLO205/VCR cells by the specific inhibitor of MEK, PD098059.The IC50 (248 +19.6 and 215 +10.7 ng/ml) to VCR of SW480/VCR andCoLo205/VCR cells exhibited a 2.16 and 2.03-fold higher resistancecompared to the negative control group (SW480 and CoLo205 cells)(115+15.6 and 106 +11.9 ng/ml), but a 1.35 and 1.21 -fold higher resistance thanthe group treated with VCR (30 ng/ml)+ PD098059 (184 + 21.8 and 177+19.4 ng/ml).CONCLUSION This study shows that the expression of P-gp can beinduced by exposuring cells to VCR, and that this induction can be reversedby inhibiting the ERK signaling pathway at the point of MEK by its specificinhibitor, PD098059. The ERK signal-transduction pathway may play a rolein modulating mdrl expression in colon cancer.
文摘The Yellow River Basin (YRB) is not only an important ecological barrier in north China,but also an important agricultural production base and energy base in China,playing a very important role in China’s economic and social development and ecological security.Under the combined actions of climate change and human activities,a series of ecological and environmental problems have emerged in the YRB,including degradation of glaciers and frozen soil,shortage of water resources,land desertification,aggravated soil and water loss,frequent floods and droughts,and reducing biodiversity.Warmer and wetter climate over the upper reaches and warmer and drier one in the middle and lower reaches have profoundly affected the ecological security of the YRB.In the future,the temperature in the YRB will continue to rise,extreme events will increase and the climate pattern of drought and water shortage will not be changed fundamentally,which will make the basin face more severe ecological security risks.For the current ecological problems and future ecological security risk challenges,it is necessary and urgent to take adaptive measures to deal with climate change and protect the ecological environment.The measures mainly include:strengthening the scientific research on the impact of climate change and extreme events on the ecological environment of the YRB and improving the ability of climate change risk management;strengthening the water conservation and protection in the upper reaches,the management of desert and soil erosion in the middle reaches and the ecological protection of water resources and wetlands in the lower reaches in the YRB and;collaborating with multiple departments to jointly tackle the ecological security challenges brought by climate change.
基金Supported by a grant from the 973 National Great Foundation Research Program of China (No. 2002CB513100).
文摘Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both mac-roscopic and microscopic observation with HE staining. Results: The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had oc-curred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia. Conclusion: A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.
