目的全面了解我国门静脉高压食管胃静脉曲张内镜治疗的现状,为我国内镜治疗的发展提供数据支持和参考。方法本研究由中国肝脏健康联盟(Liver Health Consortium in China,CHESS)发起,通过网络分发调查问卷了解2022年国内开展门静脉高压...目的全面了解我国门静脉高压食管胃静脉曲张内镜治疗的现状,为我国内镜治疗的发展提供数据支持和参考。方法本研究由中国肝脏健康联盟(Liver Health Consortium in China,CHESS)发起,通过网络分发调查问卷了解2022年国内开展门静脉高压食管胃静脉曲张内镜治疗的基本情况。问卷问题主要包括各类内镜治疗例数和适应证,依照指南预防食管胃静脉曲张出血(esophagogastric variceal bleeding,EGVB)的依从性,急性EGVB的处理、治疗时机,胃底及特殊类型静脉曲张的处理、内镜治疗的改善等方面。统计各项治疗数据的医院数量占参与调查问卷医院数量的比例,各级医院的指南依从性比较使用卡方检验进行分析。结果31个省(自治区、直辖市)共836家医院参与本调研,调查显示内镜治疗的主要适应证是控制急性出血(49.3%,412/836)和预防再出血(38.3%,320/836)。参与调研医院对于我国指南中EGVB一级预防的推荐意见(非选择性β受体阻滞剂或内镜治疗)的依从性为72.5%(606/836),对于EGVB二级预防推荐意见(非选择性β受体阻滞剂联合内镜治疗)的依从性为39.2%(328/836),三级医院和二级医院对于一级预防[71.0%(495/697)比79.9%(111/139),χ^(2)=4.11,P=0.033]和二级预防的依从性[41.6%(290/697)比27.3%(38/139),χ^(2)=9.31,P=0.002]差异有统计学意义。78.2%(654/836)的医院首选内镜治疗处理急性EGVB,三级医院首选内镜治疗的比例明显高于二级医院[82.6%(576/697)比56.1%(78/139),χ^(2)=46.33,P<0.001]。治疗时机通常为出血后12h内(48.5%,317/654)和12~24h(36.9%,241/654)。对于近贲门大弯侧的胃底静脉曲张和孤立性胃静脉曲张,分别有48.2%(403/836)和29.9%(250/836)的医院首选组织胶联合硬化剂注射进行处理,而12.4%(104/836)和26.4%(221/836)的医院首选以止血夹为基础的内镜治疗术式。参与调研医院认为提高内镜医师水平(84.2%,704/836)和对患者进行准确的术前评估并提展开更多
Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and animals.There is an urgent ne...Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and animals.There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in animals.Rabies virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health threat.The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and dogs.The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice.Notably,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer periods.Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.展开更多
Rainbow refractometry is widely used to measure the radius and real part of refractive index of a cylinder. However, studies on the detection of imaginary part of the refractive index with rainbow technique were scarc...Rainbow refractometry is widely used to measure the radius and real part of refractive index of a cylinder. However, studies on the detection of imaginary part of the refractive index with rainbow technique were scarce. This paper presents a new method for simultaneously measuring the radius, real and imaginary part of the refractive index of a cylinder, on the basis of the Airy theory and the Bouguer theory. The rainbows produced by the illuminated cylinder at a capillary exit are captured by a CCD camera in a lab- scale system, and then processed by the proposed method. Experimental results showed that the radius, real and imaginary part of the refractive index can be accurately determined when the SNR （signal to noise ratio） of the ripple structure is sufficiently high. However, the SNR of the ripple structure gradually decreases with decreasing scattering intensity of the cylinder, leading to larger measurement errors of the radius and real part of the refractive index. The relative error of the imaginary part of the refractive index derived from the measurement errors of the radius and real part of the refractive index, is less than 3.4%.展开更多
文摘目的全面了解我国门静脉高压食管胃静脉曲张内镜治疗的现状,为我国内镜治疗的发展提供数据支持和参考。方法本研究由中国肝脏健康联盟(Liver Health Consortium in China,CHESS)发起,通过网络分发调查问卷了解2022年国内开展门静脉高压食管胃静脉曲张内镜治疗的基本情况。问卷问题主要包括各类内镜治疗例数和适应证,依照指南预防食管胃静脉曲张出血(esophagogastric variceal bleeding,EGVB)的依从性,急性EGVB的处理、治疗时机,胃底及特殊类型静脉曲张的处理、内镜治疗的改善等方面。统计各项治疗数据的医院数量占参与调查问卷医院数量的比例,各级医院的指南依从性比较使用卡方检验进行分析。结果31个省(自治区、直辖市)共836家医院参与本调研,调查显示内镜治疗的主要适应证是控制急性出血(49.3%,412/836)和预防再出血(38.3%,320/836)。参与调研医院对于我国指南中EGVB一级预防的推荐意见(非选择性β受体阻滞剂或内镜治疗)的依从性为72.5%(606/836),对于EGVB二级预防推荐意见(非选择性β受体阻滞剂联合内镜治疗)的依从性为39.2%(328/836),三级医院和二级医院对于一级预防[71.0%(495/697)比79.9%(111/139),χ^(2)=4.11,P=0.033]和二级预防的依从性[41.6%(290/697)比27.3%(38/139),χ^(2)=9.31,P=0.002]差异有统计学意义。78.2%(654/836)的医院首选内镜治疗处理急性EGVB,三级医院首选内镜治疗的比例明显高于二级医院[82.6%(576/697)比56.1%(78/139),χ^(2)=46.33,P<0.001]。治疗时机通常为出血后12h内(48.5%,317/654)和12~24h(36.9%,241/654)。对于近贲门大弯侧的胃底静脉曲张和孤立性胃静脉曲张,分别有48.2%(403/836)和29.9%(250/836)的医院首选组织胶联合硬化剂注射进行处理,而12.4%(104/836)和26.4%(221/836)的医院首选以止血夹为基础的内镜治疗术式。参与调研医院认为提高内镜医师水平(84.2%,704/836)和对患者进行准确的术前评估并提
基金funded by the National Key Research and Development Program of China(grant No.2021YFC2600202)the National Natural Science Foundation of China(grant numbers 31872487).
文摘Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and animals.There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in animals.Rabies virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health threat.The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and dogs.The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice.Notably,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer periods.Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.
基金the National Natural Science Foundation of China(No.50906012)Research Award Program for Outstanding Young Teachers in Southeast University,China(No.3203001202)+2 种基金QingLan Project(No.1103000126)Scientific Research Foundation of Graduate School of Southeast University(YBJJ1220)Research and Innovation Project for College Graduates of Jiangsu Province(CLCX-0106)
文摘Rainbow refractometry is widely used to measure the radius and real part of refractive index of a cylinder. However, studies on the detection of imaginary part of the refractive index with rainbow technique were scarce. This paper presents a new method for simultaneously measuring the radius, real and imaginary part of the refractive index of a cylinder, on the basis of the Airy theory and the Bouguer theory. The rainbows produced by the illuminated cylinder at a capillary exit are captured by a CCD camera in a lab- scale system, and then processed by the proposed method. Experimental results showed that the radius, real and imaginary part of the refractive index can be accurately determined when the SNR （signal to noise ratio） of the ripple structure is sufficiently high. However, the SNR of the ripple structure gradually decreases with decreasing scattering intensity of the cylinder, leading to larger measurement errors of the radius and real part of the refractive index. The relative error of the imaginary part of the refractive index derived from the measurement errors of the radius and real part of the refractive index, is less than 3.4%.
