Background: Mancozeb, (ethylene-bis-dithiocarbamate), is an important fungicide useful against a wide range of fungus affecting ornamental plants, crops, and fruits. We aimed to evaluate the changes in the olfactory m...Background: Mancozeb, (ethylene-bis-dithiocarbamate), is an important fungicide useful against a wide range of fungus affecting ornamental plants, crops, and fruits. We aimed to evaluate the changes in the olfactory mucosa due to mancozeb toxicity and, if cytokine is active, IL-6 immunoactivity. Material and Method: In experimental group, the mancozeb (500 mg/kg) was administered with inhalation to 10 male Wistar Albino rats for five days a week. The control group (n = 10) received distilled water with spray at the same time period. The experiment was terminated after three weeks. Samples were placed in 10% formaldehyde for fixation and placed in paraffin, sections of 5 μm were prepared from paraffin blocks and stained with Hematoxylin-Eosin. Interleukin-6 (IL-6) primary antibody were used for immunohistochemical analysis. Result: In the mancozeb group, olfactory epithelial cell degeneration and apoptosis, inflammation, dilatation and congestion in the vessels were observed. IL-6 expression was increased in vascular endothelium and inflammatory cells. Conclusions: Mancozeb was thought that the increase in IL-6 expression due to the increase in cell degeneration signal was thought to affect the development of cell apoptosis and angiogenesis, and that the use of mancozeb might adversely affect the olfactory mechanism.展开更多
Rheumatoid arthritis (RA), chronic, autoimmune causing joint stiffness, pain, fatigue and functional disorders is a disease.<span><span><span style="font-family:;" "=""> <...Rheumatoid arthritis (RA), chronic, autoimmune causing joint stiffness, pain, fatigue and functional disorders is a disease.<span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">The aim of this study is to invest</span><span style="font-family:Verdana;">igate the presence of ADAMTS-8 expression in cells in synovial fluid in rheumatoid arthritis patients</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Patients with active RA were aspirated with</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> arthro synthesis from the knee joint. Blood samples were taken from the patients at the same time, plasma samples were separated. Patients with sinovial fluid treated between 2019-2020 year in the Gazi Ya<span style="white-space:nowrap;">ş</span>argil Hospital Physical Therapy and Rehabilitation Clinic were involved in the study that was consulted with knee pain and difficulty in performing their daily activities. Informed consent form was obtained from all of the patients</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(10 RA and 10 patients).</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">CRP, RF and ESH results were statistically evaluated in patients with Control and Rheumatoid arthritis.</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="fon展开更多
<span style="font-family:Verdana;">The aim of this study was to investigate the effect of rosmarinic acid against gonadal toxicity caused by cyclophosphamide, an important anticancer drug. A total of 2...<span style="font-family:Verdana;">The aim of this study was to investigate the effect of rosmarinic acid against gonadal toxicity caused by cyclophosphamide, an important anticancer drug. A total of 28 rats were divided into 4 groups, with 7 animals in each group. The groups were created as follows;Group 1 (control) (n = 7): Subjects received only 1 ml of 0.9% saline solution per day intraperitoneally for 14 days. Group 2 (Rosmarinic Acid) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid intraperitoneally for 14 days. Group 3 (Cyclophosphamide) (n = 7): Only 1 ml of 0.9% saline solution was administered intraperitoneally to the subjects for the first 7 days. Cyclophosphamide 20 mg/kg per day was administered intraperitoneally for the last 7 days (from the 8</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> day)</span><span style="font-family:Verdana;">. Group 4 (Rosmarinic Acid</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">+ Cyclophosphamide) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid daily intraperitoneally for 14 days. From the 8</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> day of the experiment to the end of the experiment (last 7 days of the experiment), 20 mg/kg cyclophosphamide was given intraperitoneally daily. At the end of the experiment, body weights of all rats were measured first. Afterwards, the weights of testicular tissue samples were measured and the averages of the weights were taken. Routine tissue follows up was performed on the testicular tissues taken. Hematoxylin-eosin staining was applied to tissue sections of 5 μm thickness. As a result of the statistical analysis, it was determined that cyclophosmadine decreased body and testicular weight, but rosmarinic acid had a protective effect on the contrary. It was concluded that cyclophosmadine caused damage to the basement membrane structure, Sertoli, Ley展开更多
文摘Background: Mancozeb, (ethylene-bis-dithiocarbamate), is an important fungicide useful against a wide range of fungus affecting ornamental plants, crops, and fruits. We aimed to evaluate the changes in the olfactory mucosa due to mancozeb toxicity and, if cytokine is active, IL-6 immunoactivity. Material and Method: In experimental group, the mancozeb (500 mg/kg) was administered with inhalation to 10 male Wistar Albino rats for five days a week. The control group (n = 10) received distilled water with spray at the same time period. The experiment was terminated after three weeks. Samples were placed in 10% formaldehyde for fixation and placed in paraffin, sections of 5 μm were prepared from paraffin blocks and stained with Hematoxylin-Eosin. Interleukin-6 (IL-6) primary antibody were used for immunohistochemical analysis. Result: In the mancozeb group, olfactory epithelial cell degeneration and apoptosis, inflammation, dilatation and congestion in the vessels were observed. IL-6 expression was increased in vascular endothelium and inflammatory cells. Conclusions: Mancozeb was thought that the increase in IL-6 expression due to the increase in cell degeneration signal was thought to affect the development of cell apoptosis and angiogenesis, and that the use of mancozeb might adversely affect the olfactory mechanism.
文摘Rheumatoid arthritis (RA), chronic, autoimmune causing joint stiffness, pain, fatigue and functional disorders is a disease.<span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">The aim of this study is to invest</span><span style="font-family:Verdana;">igate the presence of ADAMTS-8 expression in cells in synovial fluid in rheumatoid arthritis patients</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">Patients with active RA were aspirated with</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> arthro synthesis from the knee joint. Blood samples were taken from the patients at the same time, plasma samples were separated. Patients with sinovial fluid treated between 2019-2020 year in the Gazi Ya<span style="white-space:nowrap;">ş</span>argil Hospital Physical Therapy and Rehabilitation Clinic were involved in the study that was consulted with knee pain and difficulty in performing their daily activities. Informed consent form was obtained from all of the patients</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">(10 RA and 10 patients).</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">CRP, RF and ESH results were statistically evaluated in patients with Control and Rheumatoid arthritis.</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="fon
文摘<span style="font-family:Verdana;">The aim of this study was to investigate the effect of rosmarinic acid against gonadal toxicity caused by cyclophosphamide, an important anticancer drug. A total of 28 rats were divided into 4 groups, with 7 animals in each group. The groups were created as follows;Group 1 (control) (n = 7): Subjects received only 1 ml of 0.9% saline solution per day intraperitoneally for 14 days. Group 2 (Rosmarinic Acid) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid intraperitoneally for 14 days. Group 3 (Cyclophosphamide) (n = 7): Only 1 ml of 0.9% saline solution was administered intraperitoneally to the subjects for the first 7 days. Cyclophosphamide 20 mg/kg per day was administered intraperitoneally for the last 7 days (from the 8</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> day)</span><span style="font-family:Verdana;">. Group 4 (Rosmarinic Acid</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">+ Cyclophosphamide) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid daily intraperitoneally for 14 days. From the 8</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> day of the experiment to the end of the experiment (last 7 days of the experiment), 20 mg/kg cyclophosphamide was given intraperitoneally daily. At the end of the experiment, body weights of all rats were measured first. Afterwards, the weights of testicular tissue samples were measured and the averages of the weights were taken. Routine tissue follows up was performed on the testicular tissues taken. Hematoxylin-eosin staining was applied to tissue sections of 5 μm thickness. As a result of the statistical analysis, it was determined that cyclophosmadine decreased body and testicular weight, but rosmarinic acid had a protective effect on the contrary. It was concluded that cyclophosmadine caused damage to the basement membrane structure, Sertoli, Ley
