Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential ...Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential of Eucalyptus camaldulensis essential oils (EO) on CCl<sub>4</sub>-induced hepatic damage in Wistar rats. Animals were daily orally treated with the EOs extracted by hydrodistillation from powder sieved at four particle sizes (≥355, 200 - 355, 100 - 200, ≤100 μm) and those of the unsieved powder at dose of 50 mg/Kg for 7 days. Compounds that are evaluated for these activities are hydrocarbons and oxygenated terpenes that were identified and quantified by GC/MS. Activities of enzymes markers of hepatocellular damage in serum and antioxidant enzymes in the liver homogenates were measured. In this research, EOs significantly prevented the increase in serum ALT and AST (p < 0.05), total cholesterol, triglyceride and LDL-cholesterol level in acute liver damage induced by CCl<sub>4</sub> and significant increase level of plasma HDL-cholesterol. Also, significantly (p < 0.05) decreased the extent of malondialdehyde (MDA) formation and elevated the activities of superoxide dismutase (SOD) and catalase (CAT) liver in comparison to negative control group. The best antioxidant and hepatoprotective activities were those of EOs from two fine powder fractions (≤100 μm and 100 - 200 μm) was correlated to their high concentration in oxygenated terpenes (70.9% and 46.4%, respectively), when compared to the large particles (200 - 355 μm and ≥355 μm, with 33.3% and 41.8%, respectively) and unsieved powder (37.4%).展开更多
Boscia aqueous extract. senegalensis on hyperglycemia, hyperlipidemia and oxidative stress induced in rats. The aqueous extract was prepared from B. senegalensis seed powders under the extraction conditions: time (10 ...Boscia aqueous extract. senegalensis on hyperglycemia, hyperlipidemia and oxidative stress induced in rats. The aqueous extract was prepared from B. senegalensis seed powders under the extraction conditions: time (10 min), temperature (55˚C) and the ratio of mass of flour/volume of water (3/10 g/mL). Male wistar rats aged 6 to 8 weeks were acclimated and divided into 8 groups of 5 rats each: two normal control groups which received a normal standard diet (3810 kcal/kg), and six test groups which were subjected to a hyperlipidemic diet (5310 Kcal/kg). All groups of animals were fed in this manner for 8 weeks to induce hyperglycemia, hyperlipidemia and oxidative stress. After induction the animals were treated either with distilled water for the normal and negative control groups or with atorvastatin for the positive control group or with the extract of B. senegalensis. (125, 250 and 500 mg/kg) for the test groups. Fasting blood glucose was taken every 7 days during induction. After sacrifice, biochemical and oxidative stress parameters were measured. The aqueous extract of B. senegalensis significantly decreased (p B. senegalensis at a dose of 500 mg/kg has the capacity to reduce blood sugar, improve the quality of the lipid profile and reduce oxidative stress. Thus, the results reveal that the aqueous extract of B. senegalensis has powerful hypoglycemic, hypolipidemic and antioxidant properties.展开更多
Dried leaves of <i><span style="font-family:Verdana;">Eucalyptus</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span st...Dried leaves of <i><span style="font-family:Verdana;">Eucalyptus</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span style="font-family:Verdana;"> were finely grinded and fractionated</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> by sieving into four granulometric classes (<100 μm, 100 - 200 μm, 200 - 355 μm and >355 μm). The obtained powder fractions were used for essential oil (EO) extraction by hydrodistillation and their phytochemical profile and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant activities were evaluated. The mother powder (unsieved powder) was used for comparison. Particle size exerted a significant influence (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.05) on the phytochemical composition and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant prop</span><span><span style="font-family:Verdana;">erties of the EOs. Comparatively, the mother powder had the highest contents of </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-pinene (55.6%), camphene (3.4%) and limonene (3.7%), while 1,8-cineole (26.6% and 22.4%), </span></span><span style="font-family:Verdana;">exo-fenchol (5.6% and 3.5%), </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-campholenol (4.2% and 3.4%), L-trans-pinocarveol (5.5% and 2.7%), L-borneol (12.6% and 6.8%) and </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-terpineol (16.4% and 7.6%) are the main compounds of EOs from the <100 μm and 100 - 200 μm fractions, respectively. The antioxidant activities of the EOs revealed higher radical-scavenging activities DPPH (90.62% and 70.46%) and ABTS (89.59% and 73.31%) for展开更多
文摘Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential of Eucalyptus camaldulensis essential oils (EO) on CCl<sub>4</sub>-induced hepatic damage in Wistar rats. Animals were daily orally treated with the EOs extracted by hydrodistillation from powder sieved at four particle sizes (≥355, 200 - 355, 100 - 200, ≤100 μm) and those of the unsieved powder at dose of 50 mg/Kg for 7 days. Compounds that are evaluated for these activities are hydrocarbons and oxygenated terpenes that were identified and quantified by GC/MS. Activities of enzymes markers of hepatocellular damage in serum and antioxidant enzymes in the liver homogenates were measured. In this research, EOs significantly prevented the increase in serum ALT and AST (p < 0.05), total cholesterol, triglyceride and LDL-cholesterol level in acute liver damage induced by CCl<sub>4</sub> and significant increase level of plasma HDL-cholesterol. Also, significantly (p < 0.05) decreased the extent of malondialdehyde (MDA) formation and elevated the activities of superoxide dismutase (SOD) and catalase (CAT) liver in comparison to negative control group. The best antioxidant and hepatoprotective activities were those of EOs from two fine powder fractions (≤100 μm and 100 - 200 μm) was correlated to their high concentration in oxygenated terpenes (70.9% and 46.4%, respectively), when compared to the large particles (200 - 355 μm and ≥355 μm, with 33.3% and 41.8%, respectively) and unsieved powder (37.4%).
文摘Boscia aqueous extract. senegalensis on hyperglycemia, hyperlipidemia and oxidative stress induced in rats. The aqueous extract was prepared from B. senegalensis seed powders under the extraction conditions: time (10 min), temperature (55˚C) and the ratio of mass of flour/volume of water (3/10 g/mL). Male wistar rats aged 6 to 8 weeks were acclimated and divided into 8 groups of 5 rats each: two normal control groups which received a normal standard diet (3810 kcal/kg), and six test groups which were subjected to a hyperlipidemic diet (5310 Kcal/kg). All groups of animals were fed in this manner for 8 weeks to induce hyperglycemia, hyperlipidemia and oxidative stress. After induction the animals were treated either with distilled water for the normal and negative control groups or with atorvastatin for the positive control group or with the extract of B. senegalensis. (125, 250 and 500 mg/kg) for the test groups. Fasting blood glucose was taken every 7 days during induction. After sacrifice, biochemical and oxidative stress parameters were measured. The aqueous extract of B. senegalensis significantly decreased (p B. senegalensis at a dose of 500 mg/kg has the capacity to reduce blood sugar, improve the quality of the lipid profile and reduce oxidative stress. Thus, the results reveal that the aqueous extract of B. senegalensis has powerful hypoglycemic, hypolipidemic and antioxidant properties.
文摘Dried leaves of <i><span style="font-family:Verdana;">Eucalyptus</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span style="font-family:Verdana;"> were finely grinded and fractionated</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> by sieving into four granulometric classes (<100 μm, 100 - 200 μm, 200 - 355 μm and >355 μm). The obtained powder fractions were used for essential oil (EO) extraction by hydrodistillation and their phytochemical profile and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant activities were evaluated. The mother powder (unsieved powder) was used for comparison. Particle size exerted a significant influence (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.05) on the phytochemical composition and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant prop</span><span><span style="font-family:Verdana;">erties of the EOs. Comparatively, the mother powder had the highest contents of </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-pinene (55.6%), camphene (3.4%) and limonene (3.7%), while 1,8-cineole (26.6% and 22.4%), </span></span><span style="font-family:Verdana;">exo-fenchol (5.6% and 3.5%), </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-campholenol (4.2% and 3.4%), L-trans-pinocarveol (5.5% and 2.7%), L-borneol (12.6% and 6.8%) and </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-terpineol (16.4% and 7.6%) are the main compounds of EOs from the <100 μm and 100 - 200 μm fractions, respectively. The antioxidant activities of the EOs revealed higher radical-scavenging activities DPPH (90.62% and 70.46%) and ABTS (89.59% and 73.31%) for
