Background:This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes.Methods:The chemical composition of essential oils was determi...Background:This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes.Methods:The chemical composition of essential oils was determined using gas chromatography coupled mass spectrometry.For evaluated the antioxidant activity of essential oils was use tree spectrophotometric methods:diphenyl-1-picrylhydrazyl(DPPH),2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS)andβ-Carotene bleaching test.The essential oils with good activity were determined the responsible molecules using the Bioautographic HP-TLC-DPPH method.Results:The scavenging capacity of the radicals was assessed with DPPH and ABTS methods,the best results were found in the oils of M.mollis IC_(50) DPPH 2.80 mg/ml and IC_(50) ABTS 0.205 mg/mL and in A.glutinosa IC_(50) DPPH 12.972 mg/mL and IC_(50) ABTS 0.321 mg/mL,the results were compared with a pattern of natural reference in this case,the essential oil of T.vulgaris IC_(50) DPPH 0.474 mg/mL and IC_(50) ABTS 0.272 mg/mL.The evaluation of the antioxidant activity was determined by theβ-carotene bleaching test,the most notable activity results were from M.mollis IC_(50)0.119 mg/mL,A.glutinosa IC_(50)0.062 mg/mL and B.latifolia IC_(50)0,064 mg/mL.DPPH bioautography revealed the active molecules antioxidants in oils for M.mollis were thymol acetate(7.73%)and carvacrol acetate(24.52%),for A.glutinosa wasγ-muurolene(2.68%),and for B.latifolia Z-caryophyllene(2.99%),aristolochene(0.11%)and cis-cadin-4-en-7-ol(4.11%).Conclusion:The results of antioxidant activity shown in descending order that the essential oils of:M.mollis,A.glutinosa and B.latifolia,are those with the highest activity using the DPPH and ABTS methods.Theβ-Carotene bleaching test method confirms the 3 oils as the most active in the following order:A.glutinosa,B.latifolia and M.mollis.An antioxidant bioautographic study identified the molecules responsible for the activity in three essential oils with good activity.展开更多
文摘Background:This research values the antioxidant activity and its responsible molecules in six essential oils from medicinal plants in the Ecuadorian Andes.Methods:The chemical composition of essential oils was determined using gas chromatography coupled mass spectrometry.For evaluated the antioxidant activity of essential oils was use tree spectrophotometric methods:diphenyl-1-picrylhydrazyl(DPPH),2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS)andβ-Carotene bleaching test.The essential oils with good activity were determined the responsible molecules using the Bioautographic HP-TLC-DPPH method.Results:The scavenging capacity of the radicals was assessed with DPPH and ABTS methods,the best results were found in the oils of M.mollis IC_(50) DPPH 2.80 mg/ml and IC_(50) ABTS 0.205 mg/mL and in A.glutinosa IC_(50) DPPH 12.972 mg/mL and IC_(50) ABTS 0.321 mg/mL,the results were compared with a pattern of natural reference in this case,the essential oil of T.vulgaris IC_(50) DPPH 0.474 mg/mL and IC_(50) ABTS 0.272 mg/mL.The evaluation of the antioxidant activity was determined by theβ-carotene bleaching test,the most notable activity results were from M.mollis IC_(50)0.119 mg/mL,A.glutinosa IC_(50)0.062 mg/mL and B.latifolia IC_(50)0,064 mg/mL.DPPH bioautography revealed the active molecules antioxidants in oils for M.mollis were thymol acetate(7.73%)and carvacrol acetate(24.52%),for A.glutinosa wasγ-muurolene(2.68%),and for B.latifolia Z-caryophyllene(2.99%),aristolochene(0.11%)and cis-cadin-4-en-7-ol(4.11%).Conclusion:The results of antioxidant activity shown in descending order that the essential oils of:M.mollis,A.glutinosa and B.latifolia,are those with the highest activity using the DPPH and ABTS methods.Theβ-Carotene bleaching test method confirms the 3 oils as the most active in the following order:A.glutinosa,B.latifolia and M.mollis.An antioxidant bioautographic study identified the molecules responsible for the activity in three essential oils with good activity.
