<strong>Introduction: </strong>Hepatitis B virus (HBV) infection is a major cause of prenatal death worldwide. Chronic hepatitis B (CHB) infection is associated with an increased risk of cirrhosis, hepatic...<strong>Introduction: </strong>Hepatitis B virus (HBV) infection is a major cause of prenatal death worldwide. Chronic hepatitis B (CHB) infection is associated with an increased risk of cirrhosis, hepatic decomposition, and hepatocellular carcinoma (HCC). <strong>Objective:</strong> This project work surveyed the prevalence of hepatitis B among febrile patients as well as to detect hepatitis B virus in the blood and the stage of the infection of hepatitis B on the affected patients and carrier stage or state of immunity of the affected patients. <strong>Methodology:</strong> A well-designed questionnaire/checklist was used to gather information regarding age, HIV-Status, and sex from 50 febrile patients. 2 ml of blood sample was obtained by venin-puncture using a sterile hypodermic syringe and emptied into a clean dry tube (without anticoagulant) observing the necessary aseptic techniques. The blood was centrifuged and the sera obtained and stored at 2 - 8 c for HBsAg screening. Hepatitis B virus was tested using an<em> in-vitro </em>diagnostic kit called HBsAg one-step rapid test strip. The HBsAg one-step rapid test is a lateral flow chromatographic immunoassay based on the principle of the double antibody-sandwich technique. The membrane is pre-coated with anti-HBsAg antibodies on the test line region of the test. During testing, the serum specimen reacts with the particle coated with anti-HBsAg antibody. The serum moves up with capillary action to react with the coated antibody on the membrane. Then, the colored line (positive) will be generated which shows the presence of the virus. But negative shows absence of the virus. The blood in the test tube was spun using a centrifuge to separate the red cells from the serum. The test pouch, serum, and control were allowed to equilibrate to room temperature before testing. The test strip was removed from the sealed pouch and used immediately. The test strip was immersed vertically into the serum with the arrows pointing towards the serum for about 10 - 15 seconds, w展开更多
文摘<strong>Introduction: </strong>Hepatitis B virus (HBV) infection is a major cause of prenatal death worldwide. Chronic hepatitis B (CHB) infection is associated with an increased risk of cirrhosis, hepatic decomposition, and hepatocellular carcinoma (HCC). <strong>Objective:</strong> This project work surveyed the prevalence of hepatitis B among febrile patients as well as to detect hepatitis B virus in the blood and the stage of the infection of hepatitis B on the affected patients and carrier stage or state of immunity of the affected patients. <strong>Methodology:</strong> A well-designed questionnaire/checklist was used to gather information regarding age, HIV-Status, and sex from 50 febrile patients. 2 ml of blood sample was obtained by venin-puncture using a sterile hypodermic syringe and emptied into a clean dry tube (without anticoagulant) observing the necessary aseptic techniques. The blood was centrifuged and the sera obtained and stored at 2 - 8 c for HBsAg screening. Hepatitis B virus was tested using an<em> in-vitro </em>diagnostic kit called HBsAg one-step rapid test strip. The HBsAg one-step rapid test is a lateral flow chromatographic immunoassay based on the principle of the double antibody-sandwich technique. The membrane is pre-coated with anti-HBsAg antibodies on the test line region of the test. During testing, the serum specimen reacts with the particle coated with anti-HBsAg antibody. The serum moves up with capillary action to react with the coated antibody on the membrane. Then, the colored line (positive) will be generated which shows the presence of the virus. But negative shows absence of the virus. The blood in the test tube was spun using a centrifuge to separate the red cells from the serum. The test pouch, serum, and control were allowed to equilibrate to room temperature before testing. The test strip was removed from the sealed pouch and used immediately. The test strip was immersed vertically into the serum with the arrows pointing towards the serum for about 10 - 15 seconds, w
