Epidemiological studies indicate that treatment with metformin, an AMP-activated protein kinase (AMPK) activator, reduces the incidence of cancers. Activation of AMPK has also been reported to oppose tumor progression...Epidemiological studies indicate that treatment with metformin, an AMP-activated protein kinase (AMPK) activator, reduces the incidence of cancers. Activation of AMPK has also been reported to oppose tumor progression in diverse types of cancers and offers promising cancer therapy. Furthermore, AMPK is a primary regulator of energy metabolism and has also been implicated in cell cycle progression, angiogenesis, cell transformation, migration, and cancer. We have recently synthesized novel flavonoids, namely, triphenylmethanol derivatives (TPMs), but the effectiveness of the TPMs on the activity of AMPK remains unclear. We hypothesized that the novel TPMs would inhibit cancer cell proliferation through the activation of AMPK isoforms in cells. The effects of TPMs on prostate cells (PC-3) were investigated. Cells were exposed to TPMs for either 12 or 24 hr. at the respective doses of 0, 25, 50 100, and 200 µM based on the cell viability studies by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) (MTT) assay. The results indicate that cells exposed to the respective doses of TPMs increased both phospho- and total-AMPKα1 in a dose- and time-dependent manner. The effects of the increases for the phospho- and total-AMPKα in cells were greater for the 24-hr than the 12-hr. incubation. Further studies are currently going on to elucidate the specificities of the said insults in increasing the phospho- and total-AMPKα activities and for the other respective isoforms.展开更多
Femtosecond pulses from an ultrafast mode-locked fiber laser can be optimized in real time by combining single-shot spectral measurements with a smart genetic algorithm to actively control and drive the intracavity dy...Femtosecond pulses from an ultrafast mode-locked fiber laser can be optimized in real time by combining single-shot spectral measurements with a smart genetic algorithm to actively control and drive the intracavity dynamics.展开更多
Objective: Photobiomodulation (also known as Low Level Laser. LLLT or Cold Laser;Photo Medicine (PM)) has been a vital adjunct therapy in our clinical practice over 5 years, observations of improvement in cognition an...Objective: Photobiomodulation (also known as Low Level Laser. LLLT or Cold Laser;Photo Medicine (PM)) has been a vital adjunct therapy in our clinical practice over 5 years, observations of improvement in cognition and personality were noted in several patients. As a result, selected patients with Alzheimer’s Disease, vascular dementia, post-traumatic brain injury and other neuro-degenerative diseases were treated at clinical practices in Buffalo, New York;Sarasota, Florida;Lafayette, Indiana;Phoenix, Az., and Baton Rouge, La. Over 60 patients were treated with an average of 4 times over an 8-day period all reported/exhibited improvement in their condition, except that two men who were in their seventies were in robust health but had no short-term memory and no improvement was observed. However, Theralase has developed a more efficacious system which will be more efficacious, due to increased power for ATP activation. Method: Over 150 patients with the above conditions were treated in various areas (depending on diagnosis) including the prefrontal cortex, temporal lobe, Hippocampus, and Circle of Willis for duration of two and one-half minutes every 48 hours for 5 - 6 treatments. We utilized the Theralase multi-probe (905 nm/660 nm) at 60 miliwatts. It utilizes 5 - 905 nm near infra-red diodes and 4 infra-red 660 laser diodes with a peak power of 50,000 milliwatts at peak and pulse duration of 200 nanoseconds [1]. The PTSD patients were evaluated utilizing the co-occurring disorders program screening and assessment form. Conclusion: Dementia patients exhibited varying degrees of improvement in cognitive function and personality, leading to improved quality of life and decreased caregiver burden. PTSD patients’ improvement was objectively measured by formal neuropsychological testing utilizing the form. All PTSD patients scored no emotional problems after 3 - 5 treatments and all experienced overall sense of well-being. One experienced return of ability to smell he had not had for 5 years. Similar results were展开更多
Night-time OH levels have been determined for UK urban surface environments using two methods, the decay and steady state approximation methods. Measurement data from the UK National Environmental Technology Centre ar...Night-time OH levels have been determined for UK urban surface environments using two methods, the decay and steady state approximation methods. Measurement data from the UK National Environmental Technology Centre archive for four urban sites （Bristol, Harwell, London Eltham and Edinburgh） over the time period of 1996 to 2000 have been used in this study. Three reactive alkenes, namely isoprene, 1,3-butadiene and trans-2-pentene were chosen for the calculation of OH levels by the decay method. Hourly measurements of NO, NO〉 03, CO and 20 VOCs were used to determine night-time OH level using the steady state approximation method. Our results showed that the night-time OH levels were in the range of 1 × 10^3-1× 10^6 molecules/cm3 at these four urbap sites in the UK. The application of a t-test of these analyses indicated that except Bristol, there was no significant difference between the OH levels found from the decay and steady state approximation methods. Night-time levels of the OH radical appeared to peak in summer and spring time tracking the night-time 03 levels which also passed through a maximum at this time.展开更多
Thomas E.Davis(以下简称Tom)先生是笔者和世界聚脲技术的发明人Dudley J.PrimeauxⅡ(以下简称Dudley)的好友,今年2月因患急性心肌梗塞不幸去世。为了纪念这位曾经与Dudley共同创立聚脲事业的先锋,Dudley先生在2008年的"聚脲发展协...Thomas E.Davis(以下简称Tom)先生是笔者和世界聚脲技术的发明人Dudley J.PrimeauxⅡ(以下简称Dudley)的好友,今年2月因患急性心肌梗塞不幸去世。为了纪念这位曾经与Dudley共同创立聚脲事业的先锋,Dudley先生在2008年的"聚脲发展协会"第九次年会上发表了纪念文章。本文是经Dudley先生同意,并在他纪念性文章的基础上,结合笔者的亲历撰写完成的。展开更多
Irritable bowel syndrome(IBS)is the most prevalent functional gastrointestinal disorder worldwide,and the most common reason for referral to gastroenterology clinics.However,the pathophysiology is still not fully unde...Irritable bowel syndrome(IBS)is the most prevalent functional gastrointestinal disorder worldwide,and the most common reason for referral to gastroenterology clinics.However,the pathophysiology is still not fully understood and consequently current management guidelines are very symptom-specific,leading to mixed results.Here we present a study of 88 individuals with IBS who had baseline sequencing of their gut microbiome(stool samples),received targeted interventions that included dietary,supplement,prebiotic/probiotic,and lifestyle recommendations for a 30-day period,and a follow-up sequencing of their gut microbiome.The study’s objectives were to demonstrate unique metagenomic signatures across the IBS phenotypes and to validate whether metagenomic-guided interventions could lead to improvement of symptom scores in individuals with IBS.Enrolled subjects also completed a baseline and post-intervention questionnaire that assessed their symptom scores.The average symptom score of an individual with IBS at baseline was 160 and at the endpoint of the study the average symptom score of the cohort was 100.9.The mixed IBS subtype showed the most significant reduction in symptom scores across the different subtypes(average decrease by 102 points,P=0.005).The metagenomics analysis reveals shifts in the microbiome post-intervention that have been cross-validated with the literature as being associated with improvement of IBS symptoms.Given the complex nature of IBS,further studies with larger sample sizes,more targeted analyses,and a broader population cohort are needed to explore these results further.展开更多
文摘Epidemiological studies indicate that treatment with metformin, an AMP-activated protein kinase (AMPK) activator, reduces the incidence of cancers. Activation of AMPK has also been reported to oppose tumor progression in diverse types of cancers and offers promising cancer therapy. Furthermore, AMPK is a primary regulator of energy metabolism and has also been implicated in cell cycle progression, angiogenesis, cell transformation, migration, and cancer. We have recently synthesized novel flavonoids, namely, triphenylmethanol derivatives (TPMs), but the effectiveness of the TPMs on the activity of AMPK remains unclear. We hypothesized that the novel TPMs would inhibit cancer cell proliferation through the activation of AMPK isoforms in cells. The effects of TPMs on prostate cells (PC-3) were investigated. Cells were exposed to TPMs for either 12 or 24 hr. at the respective doses of 0, 25, 50 100, and 200 µM based on the cell viability studies by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) (MTT) assay. The results indicate that cells exposed to the respective doses of TPMs increased both phospho- and total-AMPKα1 in a dose- and time-dependent manner. The effects of the increases for the phospho- and total-AMPKα in cells were greater for the 24-hr than the 12-hr. incubation. Further studies are currently going on to elucidate the specificities of the said insults in increasing the phospho- and total-AMPKα activities and for the other respective isoforms.
文摘Femtosecond pulses from an ultrafast mode-locked fiber laser can be optimized in real time by combining single-shot spectral measurements with a smart genetic algorithm to actively control and drive the intracavity dynamics.
文摘Objective: Photobiomodulation (also known as Low Level Laser. LLLT or Cold Laser;Photo Medicine (PM)) has been a vital adjunct therapy in our clinical practice over 5 years, observations of improvement in cognition and personality were noted in several patients. As a result, selected patients with Alzheimer’s Disease, vascular dementia, post-traumatic brain injury and other neuro-degenerative diseases were treated at clinical practices in Buffalo, New York;Sarasota, Florida;Lafayette, Indiana;Phoenix, Az., and Baton Rouge, La. Over 60 patients were treated with an average of 4 times over an 8-day period all reported/exhibited improvement in their condition, except that two men who were in their seventies were in robust health but had no short-term memory and no improvement was observed. However, Theralase has developed a more efficacious system which will be more efficacious, due to increased power for ATP activation. Method: Over 150 patients with the above conditions were treated in various areas (depending on diagnosis) including the prefrontal cortex, temporal lobe, Hippocampus, and Circle of Willis for duration of two and one-half minutes every 48 hours for 5 - 6 treatments. We utilized the Theralase multi-probe (905 nm/660 nm) at 60 miliwatts. It utilizes 5 - 905 nm near infra-red diodes and 4 infra-red 660 laser diodes with a peak power of 50,000 milliwatts at peak and pulse duration of 200 nanoseconds [1]. The PTSD patients were evaluated utilizing the co-occurring disorders program screening and assessment form. Conclusion: Dementia patients exhibited varying degrees of improvement in cognitive function and personality, leading to improved quality of life and decreased caregiver burden. PTSD patients’ improvement was objectively measured by formal neuropsychological testing utilizing the form. All PTSD patients scored no emotional problems after 3 - 5 treatments and all experienced overall sense of well-being. One experienced return of ability to smell he had not had for 5 years. Similar results were
文摘Night-time OH levels have been determined for UK urban surface environments using two methods, the decay and steady state approximation methods. Measurement data from the UK National Environmental Technology Centre archive for four urban sites （Bristol, Harwell, London Eltham and Edinburgh） over the time period of 1996 to 2000 have been used in this study. Three reactive alkenes, namely isoprene, 1,3-butadiene and trans-2-pentene were chosen for the calculation of OH levels by the decay method. Hourly measurements of NO, NO〉 03, CO and 20 VOCs were used to determine night-time OH level using the steady state approximation method. Our results showed that the night-time OH levels were in the range of 1 × 10^3-1× 10^6 molecules/cm3 at these four urbap sites in the UK. The application of a t-test of these analyses indicated that except Bristol, there was no significant difference between the OH levels found from the decay and steady state approximation methods. Night-time levels of the OH radical appeared to peak in summer and spring time tracking the night-time 03 levels which also passed through a maximum at this time.
基金This work was supported by the National Institutes of Health(Grant No.R01AI151059).
文摘Irritable bowel syndrome(IBS)is the most prevalent functional gastrointestinal disorder worldwide,and the most common reason for referral to gastroenterology clinics.However,the pathophysiology is still not fully understood and consequently current management guidelines are very symptom-specific,leading to mixed results.Here we present a study of 88 individuals with IBS who had baseline sequencing of their gut microbiome(stool samples),received targeted interventions that included dietary,supplement,prebiotic/probiotic,and lifestyle recommendations for a 30-day period,and a follow-up sequencing of their gut microbiome.The study’s objectives were to demonstrate unique metagenomic signatures across the IBS phenotypes and to validate whether metagenomic-guided interventions could lead to improvement of symptom scores in individuals with IBS.Enrolled subjects also completed a baseline and post-intervention questionnaire that assessed their symptom scores.The average symptom score of an individual with IBS at baseline was 160 and at the endpoint of the study the average symptom score of the cohort was 100.9.The mixed IBS subtype showed the most significant reduction in symptom scores across the different subtypes(average decrease by 102 points,P=0.005).The metagenomics analysis reveals shifts in the microbiome post-intervention that have been cross-validated with the literature as being associated with improvement of IBS symptoms.Given the complex nature of IBS,further studies with larger sample sizes,more targeted analyses,and a broader population cohort are needed to explore these results further.
