The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological technique...The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological techniques (30 Bacillus and 30 Lactobacillus) and the ability to produce biosurfactants was demonstrated by a hydrocarbon emulsification index (E24). The emulsification indexes (E24) varied from 9% to 100% for Bacillus and from 33% to 100% for Lactobacillus as well. The antagonistic assay showed that biosurfactants were able to inhibit the formation of biofilms and growth of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhirium, Shigella boydii and Proteus mirabilis. The biosurfactant consortium (BioC) from Bacillus consortium and from Lactobacillus was able to inhibit biofilm formation and the pathogens growth. The BioC was stable to alkaline pH and the temperatures stability of Biosurfactant was ranging from 50°C to 90°C. The soap was made by the cold saponification process using one biosurfactant consortium formulated. This soap has a pH of 10 and showed good cleaning power and good foam stability. Similarly, the soap showed good antiseptic power and disinfection power against all pathogens tested. Handwashing is critical to preventing disease transmission. The persistence of pathogens in waste water was evaluated. The BioS produced showed good disinfection power against all pathogens tested. The valor of reduction on the hands and in the waste water was significantly more than compared to the control soaps used. This soap could be used in the prevention, fighting, and treatment of bacterial and viral infections.展开更多
According to the World Health Organization, foodborne diseases are a major public health problem, particularly in developing countries including the Republic of Congo. They are responsible for several episodes of diar...According to the World Health Organization, foodborne diseases are a major public health problem, particularly in developing countries including the Republic of Congo. They are responsible for several episodes of diarrhea, especially in children under five years old. There is no reliable epidemiological data on the pathogenicity of the Shigella spp. strains circulating in the whole Republic of Congo drafting this paper. The purpose of this study was to examine the Shigella spp strain pathogenicity close to an environment contaminated with faeces in the city of Brazzaville. As a result, 54 isolates have been associated with Shigella spp. The gastric acid resistance test performed on Shigella Environmental Strain (SES) and Shigella Clinical Strains (SCS) resulted in 38.8% (21/54) and 100% acid resistant, respectively. Shigella spp. Strains (SES and SCS) were ranged in a survival percentage from 11% to 93%. By monitoring Biosurfactant-Like Molecule (BLM) production, we showed that the BLM production of SES and SCS was highly dependent on bacterial culture density involving the Quorum Sensing (QS). S. flexneri, S. boydii, and S. sonnei and as well as SES and SCS were able to invade and contaminate eggs by colonizing egg yolk. The counting bacteria were ranging from two to 5 × 10<sup>7</sup> CFU/mL after contamination. Concomitantly, BLM was secreted during the post contamination of poultry eggs with 100% EI24. Further by trying to show the pathogenicity by the hemolysis test, we have shown that SES and SCS were able to show significant areas of lysis on blood agar. Finally, this work has proposed an additional model of cell invasion including biosurfactants during the pre- and post-invasion phases.展开更多
The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This...The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This work aimed to assess prevalence of beta-lactamase produced by enterobacterial isolates. Then, disc diffusion, double disc synergy test (DDST) and combined disc test (CDT) were respectively used for antimicrobial resistance, detection of Extended-Spectrum Beta-Lactamases (ESBL) and Metallo-Beta-Lactamases (MBL). bla genes were detected by PCR. A total of 132 enterobacterial strains were studied. Resistance to antibiotic families was observed with a greater frequency than 50%. Gentamicin was the least active beta-lactam antibiotic, with a resistance rate of 88%. 40.9% of strains show an ESBL phenotype and 16.6% were MBL. An overall prevalence of 74% (40/54) and respectively rates of 29.6%, 27.7% and 16.7% for blaSHV, blaCTX and blaTEM genes were observed. SHV, CTX, CTX/SHV/TEM, CTX/TEM, SHV/TEM and CTX/SHV were different ESBL genotypes observed. ESBL-producing enterobacteria isolation worried about the future of antimicrobial therapy in the Republic of Congo. This is a public health problem that requires careful monitoring and implementation of a policy of rational antibiotics use.展开更多
文摘The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological techniques (30 Bacillus and 30 Lactobacillus) and the ability to produce biosurfactants was demonstrated by a hydrocarbon emulsification index (E24). The emulsification indexes (E24) varied from 9% to 100% for Bacillus and from 33% to 100% for Lactobacillus as well. The antagonistic assay showed that biosurfactants were able to inhibit the formation of biofilms and growth of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhirium, Shigella boydii and Proteus mirabilis. The biosurfactant consortium (BioC) from Bacillus consortium and from Lactobacillus was able to inhibit biofilm formation and the pathogens growth. The BioC was stable to alkaline pH and the temperatures stability of Biosurfactant was ranging from 50°C to 90°C. The soap was made by the cold saponification process using one biosurfactant consortium formulated. This soap has a pH of 10 and showed good cleaning power and good foam stability. Similarly, the soap showed good antiseptic power and disinfection power against all pathogens tested. Handwashing is critical to preventing disease transmission. The persistence of pathogens in waste water was evaluated. The BioS produced showed good disinfection power against all pathogens tested. The valor of reduction on the hands and in the waste water was significantly more than compared to the control soaps used. This soap could be used in the prevention, fighting, and treatment of bacterial and viral infections.
文摘According to the World Health Organization, foodborne diseases are a major public health problem, particularly in developing countries including the Republic of Congo. They are responsible for several episodes of diarrhea, especially in children under five years old. There is no reliable epidemiological data on the pathogenicity of the Shigella spp. strains circulating in the whole Republic of Congo drafting this paper. The purpose of this study was to examine the Shigella spp strain pathogenicity close to an environment contaminated with faeces in the city of Brazzaville. As a result, 54 isolates have been associated with Shigella spp. The gastric acid resistance test performed on Shigella Environmental Strain (SES) and Shigella Clinical Strains (SCS) resulted in 38.8% (21/54) and 100% acid resistant, respectively. Shigella spp. Strains (SES and SCS) were ranged in a survival percentage from 11% to 93%. By monitoring Biosurfactant-Like Molecule (BLM) production, we showed that the BLM production of SES and SCS was highly dependent on bacterial culture density involving the Quorum Sensing (QS). S. flexneri, S. boydii, and S. sonnei and as well as SES and SCS were able to invade and contaminate eggs by colonizing egg yolk. The counting bacteria were ranging from two to 5 × 10<sup>7</sup> CFU/mL after contamination. Concomitantly, BLM was secreted during the post contamination of poultry eggs with 100% EI24. Further by trying to show the pathogenicity by the hemolysis test, we have shown that SES and SCS were able to show significant areas of lysis on blood agar. Finally, this work has proposed an additional model of cell invasion including biosurfactants during the pre- and post-invasion phases.
文摘The improper use of antimicrobials against infectious diseases has allowed microorganisms to develop defense mechanisms that give them insensitivity to these agents. All bacteria are concerned by this phenomenon. This work aimed to assess prevalence of beta-lactamase produced by enterobacterial isolates. Then, disc diffusion, double disc synergy test (DDST) and combined disc test (CDT) were respectively used for antimicrobial resistance, detection of Extended-Spectrum Beta-Lactamases (ESBL) and Metallo-Beta-Lactamases (MBL). bla genes were detected by PCR. A total of 132 enterobacterial strains were studied. Resistance to antibiotic families was observed with a greater frequency than 50%. Gentamicin was the least active beta-lactam antibiotic, with a resistance rate of 88%. 40.9% of strains show an ESBL phenotype and 16.6% were MBL. An overall prevalence of 74% (40/54) and respectively rates of 29.6%, 27.7% and 16.7% for blaSHV, blaCTX and blaTEM genes were observed. SHV, CTX, CTX/SHV/TEM, CTX/TEM, SHV/TEM and CTX/SHV were different ESBL genotypes observed. ESBL-producing enterobacteria isolation worried about the future of antimicrobial therapy in the Republic of Congo. This is a public health problem that requires careful monitoring and implementation of a policy of rational antibiotics use.
