Background Unruptured intracranial aneurysm treatment aims to reduce the risk of aneurysm rupture and bleeding,relieves symptoms and improve the quality of life for patients.This study aimed to assess the safety and e...Background Unruptured intracranial aneurysm treatment aims to reduce the risk of aneurysm rupture and bleeding,relieves symptoms and improve the quality of life for patients.This study aimed to assess the safety and efficacy of Pipeline Embolization Device(PED,Covidien/Medtronic,Irvine,CA)treatment for intracranial aneurysms presenting with mass effect in real-world settings.Methods We selected patients from the PED in China Post-Market Multi-Center Registry Study with mass effect presentation.The study endpoints included postoperative mass effect deterioration and mass effect relief at follow-up(3–36 months).We conducted multivariate analysis to identify factors associated with mass effect relief.Subgroup analyses by aneurysm location,size and form were also performed.Results This study included 218 patients with a mean age of 54.3±11.8 years and a female predominance of 74.0%(162/218).The postoperative mass effect deterioration rate was 9.6%(21/218).During a median follow-up period of 8.4 months,the mass effect relief rate was 71.6%(156/218).Notably,immediate aneurysm occlusion following treatment was significantly associated with mass effect relief(OR 0.392,95%CI,0.170 to 0.907,p=0.029).Subgroup analysis demonstrated that adjunctive coiling contributed to mass effect relief in cavernous aneurysms,while dense embolism impeded symptom relief in aneurysms<10mm and saccular aneurysms.Conclusions Our data confirmed the efficacy of PED in relieving mass effect.The findings of this study provide support for endovascular treatment to alleviate mass effect in unruptured intracranial aneurysms.Trial registration number NCT03831672.展开更多
Humanβ-galactosidase(β-gal)is recognized as a crucial biomarker for evaluating senescence at the cellular and tissue levels in humans.However,tools to precisely track the endogenousβ-gal are still limited.Herein,we...Humanβ-galactosidase(β-gal)is recognized as a crucial biomarker for evaluating senescence at the cellular and tissue levels in humans.However,tools to precisely track the endogenousβ-gal are still limited.Herein,we present two novel self-calibratingβ-gal probes 7a and 7b which were constructed on a unique green/red dual-emissive fluorescence platform.The two probes inherently exhibited a stable green fluorescence signal impervious toβ-gal activity,serving as a reliable internal reference.They also displayed a progressively diminishing red fluorescence signal with the increasing ofβ-gal expression levels.The dual behavior endows them with self-calibration capacity and then renders excellently selective and sensitive for precisely monitoringβ-gal activity.Notably,compared with E.coliβ-gal,the two probes are more effectively response to A.oryzaeβ-gal homologous to humanβ-gal,indicating their unique species-selectivity.Furthermore,7a was validated for its effectiveness in determining senescenceassociatedβ-galactosidase(SA-β-gal)expression in senescent NRK-52E and HepG2 cells,underscoring its practical applicability in senescence research.展开更多
Two different initialization schemes for tropical cyclone(TC)prediction in numerical models are evaluated based on a case study of Typhoon Lekima(2019).The first is a dynamical initialization(DI)scheme where the axisy...Two different initialization schemes for tropical cyclone(TC)prediction in numerical models are evaluated based on a case study of Typhoon Lekima(2019).The first is a dynamical initialization(DI)scheme where the axisymmetric TC vortex in the initial conditions is spun up through the 6-h cycle runs before the initial forecast time.The second scheme is a bogussing scheme where the analysis TC vortex is replaced by a synthetic Rankine vortex.Results show that although both initialization schemes can help improve the simulated rapid intensification(RI)of Lekima,the simulation employing the DI scheme(DIS)reproduces better the RI onset and intensification rate than that employing the bogussing scheme(BOG).Further analyses show the cycle runs of DI help establish a realistic TC structure with stronger secondary circulation than those in the control run and BOG,leading to fast vortex spinup and contraction of the radius of maximum wind(RMW).The resultant strong inner-core primary circulation favors precession of the midlevel vortex under the moderate vertical wind shear(VWS)and thus helps vortex alignment,contributing to an earlier RI onset.Afterwards,the decreased vertical shear and the stronger convection inside the RMW support the persistent RI of Lekima in DIS.In contrast,the reduced VWS is not well captured and the inner-core convection is weaker and resides farther away from the TC center in BOG,leading to slower intensification.The results imply that the DI effectively improves the prediction of the inner-core process,which is crucial to the RI forecast.展开更多
Objectives The aim of this study was to compare complications and outcomes between intracranial aneurysms treated with the Pipeline embolisation device(PED)alone or with PED combined with coiling for different-sized a...Objectives The aim of this study was to compare complications and outcomes between intracranial aneurysms treated with the Pipeline embolisation device(PED)alone or with PED combined with coiling for different-sized aneurysms.Method Patients with aneurysms treated by PED were collected from the PED in China postmarket multicentre registry study.We performed a propensity match analysis to compare the efficacy and safety between PED alone and PED combined with coiling treatment,and then aneurysms were organised into three groups based on their size:small(≤7 mm),medium(≤15 mm to>7 mm)and large/giant(>15 mm).Complications and aneurysm occlusion rates in the aneurysm size groups were compared between PED alone and PED combined with coiling patients.Result A total of 1171 patients with 1322 aneurysms were included.All patients received clinical follow-up,while angiographic follow-up was available in 967 aneurysms.For small aneurysms,there was no difference in the aneurysm occlusion rate between two groups(79.1%vs 88.4%,respectively),while there was a significant increase in the ischaemic complication rate(8.3%vs 19.3%,respectively,p=0.0001).For medium and large/giant saccular aneurysms,PED combined with coiling significantly improved the occlusion rate(medium aneurysms:74.7%vs 88.8%,respectively,p<0.0001;large/giant saccular aneurysms:72.9%vs 86.9%,respectively,p=0.018),while there were no differences in the total complication rate.For large/giant non-saccular aneurysms,two groups showed no differences.Conclusion Use of the PED with adjunctive coils can significantly improve the occlusion rate of medium aneurysms,without increasing the total complication rate.展开更多
This study reexamines the correlation between the size and intensity of tropical cyclones(TCs) over the western North Pacific from the perspective of individual TCs, rather than the previous large-sample framework mix...This study reexamines the correlation between the size and intensity of tropical cyclones(TCs) over the western North Pacific from the perspective of individual TCs, rather than the previous large-sample framework mixing up all TC records.Statistics show that the positive size-intensity correlation based on individual TCs is relatively high. However, this correlation is obscured by mixing large samples. The weakened correlation based on all TC records is primarily due to the diversity in the size change relative to the same intensity change among TCs, which can be quantitatively measured by the linear regression coefficient(RC) of size against intensity. To further explore the factors that cause the variability in RCs that weakens the size-intensity correlation when considering all TC records, the TCs from 2001 to 2020 are classified into two groups according to their RC magnitudes, within which the high-RC TCs have a larger size expansion than the low-RC TCs given the same intensity change. Two key mechanisms responsible for the RC differences are proposed. First, the highRC TCs are generally located at higher latitudes than the low-RC TCs, resulting in higher planetary vorticity and thus higher planetary angular momentum import at low levels. Second, the high-RC TCs are susceptible to stronger environmental vertical wind shear, leading to more prolific outer convection than the low-RC TCs. The positive feedback between outer diabatic heating and boundary layer inflow favors the inward import of absolute angular momentum in the outer region, thereby contributing to a larger size expansion in the high-RC TCs.展开更多
This study investigates the modulation of initial wind field structure on the relationship between the size and intensity of a simulated vortex.A series of idealized experiments are conducted by varying the radius of ...This study investigates the modulation of initial wind field structure on the relationship between the size and intensity of a simulated vortex.A series of idealized experiments are conducted by varying the radius of maximum wind(RMW)and shape parameter of the initial vortices.The size–intensity relationship is quantified by the linear regression coefficient of the azimuthally-averaged gale-force wind radius against the maximum wind during the development stage,reflecting the degree of size expansion at the same intensity increment.The regression coefficient increases with increased RMW and decreased,with the RMW being the primary constraint.The effect of lowering on the elevation of the regression coefficient gradually stands out when the initial RMW is large.Enlarging the RMW leads to a secondary circulation with a horizontally elongated structure,which retards the intensification while promoting size expansion,thus substantially enhancing size expansion as the vortex intensifies.Broadening the wind field outside the RMW by reducing results in abounding convection in the outer region,which promotes size expansion.Based on the axisymmetric tangential wind tendency and Sawyer–Eliassen equations,when the RMW is large,the active convection in the outer region can weaken the radial inflow induced by the eyewall heating in the inner region,thus retarding the intensification by reducing the radial imports of vorticity near the RMW.展开更多
Dear Editor,Targeting senescence therapy is a promising anticancer strategy by arresting the cell-cycle and inducing cellular senescence.However,senescent cells can promote malignancy and drug resistance.1,2 Thus,with...Dear Editor,Targeting senescence therapy is a promising anticancer strategy by arresting the cell-cycle and inducing cellular senescence.However,senescent cells can promote malignancy and drug resistance.1,2 Thus,with the induction of senescence emerged as a viable therapeutic concept,it is important to consider how to eliminate those senescent cancer cells.展开更多
Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syn...Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syndrome(RSS)affected sex-linked dwarf(SLD)chickens compared to SLD chickens.However,the association between BCL6 gene and hematopoietic function remains unknown in chickens.Methods:In this study,we used RSS affected SLD(RSS-SLD)chickens,SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells(HSCs),to investigate the effect of the BCL6 on differentiation and development of HSCs.Results:The results showed that comparison of RSS-SLD chickens with SLD chickens,the BCL6 was highly expressed in RSS-SLD chickens bone marrow.The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow,bone density was reduced,and the levels of immature erythrocytes in peripheral blood were increased.At the same time,the hematopoietic function of HSCs decreased in RSS-SLD chickens,which was manifested by a decrease in the hematopoietic growth factors(HGFs)EPO,SCF,TPO,and IL-3,as well as hemoglobinα1 and hemoglobinβexpression.Moreover,mitochondrial function in the HSCs of RSS-SLD chickens was damaged,including an increase in ROS production,decrease in ATP concentration,and decrease in mitochondrial membrane potential(ΔΨm).The same results were also observed in SLD chickens compared with normal chickens;however,the symptoms were more serious in RSS-SLD chickens.Additionally,after overexpression of the BCL6 in primary HSCs,the secretion of HGFs(EPO,SCF,TPO and IL-3)was inhibited and the expression of hemoglobinα1 and hemoglobinβwas decreased.However,cell proliferation was accelerated,apoptosis was inhibited,and the HSCs entered a cancerous state.The function of mitochondria was also abnormal,ROS production was decreased,and ATP concentration andΔΨm were increased,which was related to the inhibition of apoptosis of stem ce展开更多
Hydrazine,an essential chemical,has been used within a wide spectrum of industries,including pesticides,pharmaceuticals and even satellite-launching systems.However,the excessive consumption of hydrazine raised the ri...Hydrazine,an essential chemical,has been used within a wide spectrum of industries,including pesticides,pharmaceuticals and even satellite-launching systems.However,the excessive consumption of hydrazine raised the risk of environmental pollution accidents and occurrence of diseases because of its high toxicity and volatility.This led to the discovery of diverse fluorescent probes for the monitoring of the dangerous substance,including those based on organic small molecules and emerging nanomaterials.Herein,we are going to present a comprehensive review of recently reported hydrazine fluorescent probes,and discuss their structure design strategies and detection mechanisms.In particular,both organic small-molecule and nanomaterial fluorescent probes for hydrazine will be discussed together for the first time.展开更多
Monoamine oxidase A(MAO-A) is a prominent myocardial source of reactive oxygen species(ROS), and its expression and activity are strongly increased in failing hearts. Therefore, accurate evaluation of MAOA activity in...Monoamine oxidase A(MAO-A) is a prominent myocardial source of reactive oxygen species(ROS), and its expression and activity are strongly increased in failing hearts. Therefore, accurate evaluation of MAOA activity in cardiomyocytes is of great importance for understanding its biological functions and early diagnosing the progression of heart failure. However, so far, there is no report on the fluorescent diagnosis of heart failure by a specific probe for MAO-A. In this work, two far-red emissive fluorescent turn-on probes(KXS-M1 and KXS-M2) for the highly selective and sensitive detection of MAO-A were fabricated.Both probes exhibit good response to MAO-A, one of which, KXS-M2, performs better than the other one in terms of a fluorescence increment and sensitivity. Using the pioneering probe KXS-M2, specific fluorescence imaging of MAO-A in glucose-deprived H9c2 cardiac cells, zebrafish and isoprenaline-induced failing heart tissues was achieved, proving that KXS-M2 can serve as a powerful tool for the diagnosis and treatment of heart failure.展开更多
基金sponsored by the National Natural Science Foundation of China(82071302)Bai Qian Wan Talent Plan(2017A07)and Beijing Municipal Administration of Hospital Incubating Program(PX2016034).
文摘Background Unruptured intracranial aneurysm treatment aims to reduce the risk of aneurysm rupture and bleeding,relieves symptoms and improve the quality of life for patients.This study aimed to assess the safety and efficacy of Pipeline Embolization Device(PED,Covidien/Medtronic,Irvine,CA)treatment for intracranial aneurysms presenting with mass effect in real-world settings.Methods We selected patients from the PED in China Post-Market Multi-Center Registry Study with mass effect presentation.The study endpoints included postoperative mass effect deterioration and mass effect relief at follow-up(3–36 months).We conducted multivariate analysis to identify factors associated with mass effect relief.Subgroup analyses by aneurysm location,size and form were also performed.Results This study included 218 patients with a mean age of 54.3±11.8 years and a female predominance of 74.0%(162/218).The postoperative mass effect deterioration rate was 9.6%(21/218).During a median follow-up period of 8.4 months,the mass effect relief rate was 71.6%(156/218).Notably,immediate aneurysm occlusion following treatment was significantly associated with mass effect relief(OR 0.392,95%CI,0.170 to 0.907,p=0.029).Subgroup analysis demonstrated that adjunctive coiling contributed to mass effect relief in cavernous aneurysms,while dense embolism impeded symptom relief in aneurysms<10mm and saccular aneurysms.Conclusions Our data confirmed the efficacy of PED in relieving mass effect.The findings of this study provide support for endovascular treatment to alleviate mass effect in unruptured intracranial aneurysms.Trial registration number NCT03831672.
基金the financial support from the National Natural Science Foundation of China(Nos.21977082,22037002and 21472148)the Natural Science Basic Research Program of Shaanxi(No.2020JC-38)。
文摘Humanβ-galactosidase(β-gal)is recognized as a crucial biomarker for evaluating senescence at the cellular and tissue levels in humans.However,tools to precisely track the endogenousβ-gal are still limited.Herein,we present two novel self-calibratingβ-gal probes 7a and 7b which were constructed on a unique green/red dual-emissive fluorescence platform.The two probes inherently exhibited a stable green fluorescence signal impervious toβ-gal activity,serving as a reliable internal reference.They also displayed a progressively diminishing red fluorescence signal with the increasing ofβ-gal expression levels.The dual behavior endows them with self-calibration capacity and then renders excellently selective and sensitive for precisely monitoringβ-gal activity.Notably,compared with E.coliβ-gal,the two probes are more effectively response to A.oryzaeβ-gal homologous to humanβ-gal,indicating their unique species-selectivity.Furthermore,7a was validated for its effectiveness in determining senescenceassociatedβ-galactosidase(SA-β-gal)expression in senescent NRK-52E and HepG2 cells,underscoring its practical applicability in senescence research.
基金supported by the National Natural Science Foundation of China(Grant Nos.41775063 and 41975071)。
文摘Two different initialization schemes for tropical cyclone(TC)prediction in numerical models are evaluated based on a case study of Typhoon Lekima(2019).The first is a dynamical initialization(DI)scheme where the axisymmetric TC vortex in the initial conditions is spun up through the 6-h cycle runs before the initial forecast time.The second scheme is a bogussing scheme where the analysis TC vortex is replaced by a synthetic Rankine vortex.Results show that although both initialization schemes can help improve the simulated rapid intensification(RI)of Lekima,the simulation employing the DI scheme(DIS)reproduces better the RI onset and intensification rate than that employing the bogussing scheme(BOG).Further analyses show the cycle runs of DI help establish a realistic TC structure with stronger secondary circulation than those in the control run and BOG,leading to fast vortex spinup and contraction of the radius of maximum wind(RMW).The resultant strong inner-core primary circulation favors precession of the midlevel vortex under the moderate vertical wind shear(VWS)and thus helps vortex alignment,contributing to an earlier RI onset.Afterwards,the decreased vertical shear and the stronger convection inside the RMW support the persistent RI of Lekima in DIS.In contrast,the reduced VWS is not well captured and the inner-core convection is weaker and resides farther away from the TC center in BOG,leading to slower intensification.The results imply that the DI effectively improves the prediction of the inner-core process,which is crucial to the RI forecast.
基金sponsored by National Natural Science Foundation of China(grant numbers:81220108007,81801156,81801158,81471167 and 81671139).
文摘Objectives The aim of this study was to compare complications and outcomes between intracranial aneurysms treated with the Pipeline embolisation device(PED)alone or with PED combined with coiling for different-sized aneurysms.Method Patients with aneurysms treated by PED were collected from the PED in China postmarket multicentre registry study.We performed a propensity match analysis to compare the efficacy and safety between PED alone and PED combined with coiling treatment,and then aneurysms were organised into three groups based on their size:small(≤7 mm),medium(≤15 mm to>7 mm)and large/giant(>15 mm).Complications and aneurysm occlusion rates in the aneurysm size groups were compared between PED alone and PED combined with coiling patients.Result A total of 1171 patients with 1322 aneurysms were included.All patients received clinical follow-up,while angiographic follow-up was available in 967 aneurysms.For small aneurysms,there was no difference in the aneurysm occlusion rate between two groups(79.1%vs 88.4%,respectively),while there was a significant increase in the ischaemic complication rate(8.3%vs 19.3%,respectively,p=0.0001).For medium and large/giant saccular aneurysms,PED combined with coiling significantly improved the occlusion rate(medium aneurysms:74.7%vs 88.8%,respectively,p<0.0001;large/giant saccular aneurysms:72.9%vs 86.9%,respectively,p=0.018),while there were no differences in the total complication rate.For large/giant non-saccular aneurysms,two groups showed no differences.Conclusion Use of the PED with adjunctive coils can significantly improve the occlusion rate of medium aneurysms,without increasing the total complication rate.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41975071,42175073)the open project of the Shanghai Typhoon Institute,China Meteorological Administration (TFJJ202003)。
文摘This study reexamines the correlation between the size and intensity of tropical cyclones(TCs) over the western North Pacific from the perspective of individual TCs, rather than the previous large-sample framework mixing up all TC records.Statistics show that the positive size-intensity correlation based on individual TCs is relatively high. However, this correlation is obscured by mixing large samples. The weakened correlation based on all TC records is primarily due to the diversity in the size change relative to the same intensity change among TCs, which can be quantitatively measured by the linear regression coefficient(RC) of size against intensity. To further explore the factors that cause the variability in RCs that weakens the size-intensity correlation when considering all TC records, the TCs from 2001 to 2020 are classified into two groups according to their RC magnitudes, within which the high-RC TCs have a larger size expansion than the low-RC TCs given the same intensity change. Two key mechanisms responsible for the RC differences are proposed. First, the highRC TCs are generally located at higher latitudes than the low-RC TCs, resulting in higher planetary vorticity and thus higher planetary angular momentum import at low levels. Second, the high-RC TCs are susceptible to stronger environmental vertical wind shear, leading to more prolific outer convection than the low-RC TCs. The positive feedback between outer diabatic heating and boundary layer inflow favors the inward import of absolute angular momentum in the outer region, thereby contributing to a larger size expansion in the high-RC TCs.
基金This study is supported by the National Natural Science Foundation of China(Grant Nos.42175073 and 41975071).
文摘This study investigates the modulation of initial wind field structure on the relationship between the size and intensity of a simulated vortex.A series of idealized experiments are conducted by varying the radius of maximum wind(RMW)and shape parameter of the initial vortices.The size–intensity relationship is quantified by the linear regression coefficient of the azimuthally-averaged gale-force wind radius against the maximum wind during the development stage,reflecting the degree of size expansion at the same intensity increment.The regression coefficient increases with increased RMW and decreased,with the RMW being the primary constraint.The effect of lowering on the elevation of the regression coefficient gradually stands out when the initial RMW is large.Enlarging the RMW leads to a secondary circulation with a horizontally elongated structure,which retards the intensification while promoting size expansion,thus substantially enhancing size expansion as the vortex intensifies.Broadening the wind field outside the RMW by reducing results in abounding convection in the outer region,which promotes size expansion.Based on the axisymmetric tangential wind tendency and Sawyer–Eliassen equations,when the RMW is large,the active convection in the outer region can weaken the radial inflow induced by the eyewall heating in the inner region,thus retarding the intensification by reducing the radial imports of vorticity near the RMW.
基金supported by the National Natural Science Foundation of China(Grant Nos.81820108022,22177076,22037002)Innovation Program of Shanghai Municipal Education Commission(2019-01-07-00-10-E00056,2021-01-07-00-02-E00104)+4 种基金The Scientific and Technological Innovation Action Plan of Science and Technology Commission of Shanghai(20JC1411300)Shanghai Frontiers Science Center of Disease and Syndrome Biology of Inflammatory Cancer Transformation(2021KJ03-12)National Thirteenth Five-Year Science and Technology Major Special Project for New Drug and Development(2017ZX09304001)the Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism(2021 Sci&Tech 03-28)the Innovative Research Team of High-level Local Universities in Shanghai and the Chinese Special Fund for State Key Laboratory of Bioreactor Engineering(2060204).
文摘Dear Editor,Targeting senescence therapy is a promising anticancer strategy by arresting the cell-cycle and inducing cellular senescence.However,senescent cells can promote malignancy and drug resistance.1,2 Thus,with the induction of senescence emerged as a viable therapeutic concept,it is important to consider how to eliminate those senescent cancer cells.
基金This work was supported by grants from the Key-Area Research and Development Program of Guangdong Province(Grant No.2020B020222002)the Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry,National Natural Science Foundation of China(Grant No.31401046)+1 种基金the China Agriculture Research System(CARS-41-G03)Guangdong Youth Talent Project.
文摘Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syndrome(RSS)affected sex-linked dwarf(SLD)chickens compared to SLD chickens.However,the association between BCL6 gene and hematopoietic function remains unknown in chickens.Methods:In this study,we used RSS affected SLD(RSS-SLD)chickens,SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells(HSCs),to investigate the effect of the BCL6 on differentiation and development of HSCs.Results:The results showed that comparison of RSS-SLD chickens with SLD chickens,the BCL6 was highly expressed in RSS-SLD chickens bone marrow.The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow,bone density was reduced,and the levels of immature erythrocytes in peripheral blood were increased.At the same time,the hematopoietic function of HSCs decreased in RSS-SLD chickens,which was manifested by a decrease in the hematopoietic growth factors(HGFs)EPO,SCF,TPO,and IL-3,as well as hemoglobinα1 and hemoglobinβexpression.Moreover,mitochondrial function in the HSCs of RSS-SLD chickens was damaged,including an increase in ROS production,decrease in ATP concentration,and decrease in mitochondrial membrane potential(ΔΨm).The same results were also observed in SLD chickens compared with normal chickens;however,the symptoms were more serious in RSS-SLD chickens.Additionally,after overexpression of the BCL6 in primary HSCs,the secretion of HGFs(EPO,SCF,TPO and IL-3)was inhibited and the expression of hemoglobinα1 and hemoglobinβwas decreased.However,cell proliferation was accelerated,apoptosis was inhibited,and the HSCs entered a cancerous state.The function of mitochondria was also abnormal,ROS production was decreased,and ATP concentration andΔΨm were increased,which was related to the inhibition of apoptosis of stem ce
基金support from the National Natural Science Foundation of China(21977082 and 22037002)the Natural Science Basic Research Program of Shaanxi(2020JC-38).
文摘Hydrazine,an essential chemical,has been used within a wide spectrum of industries,including pesticides,pharmaceuticals and even satellite-launching systems.However,the excessive consumption of hydrazine raised the risk of environmental pollution accidents and occurrence of diseases because of its high toxicity and volatility.This led to the discovery of diverse fluorescent probes for the monitoring of the dangerous substance,including those based on organic small molecules and emerging nanomaterials.Herein,we are going to present a comprehensive review of recently reported hydrazine fluorescent probes,and discuss their structure design strategies and detection mechanisms.In particular,both organic small-molecule and nanomaterial fluorescent probes for hydrazine will be discussed together for the first time.
基金supported by the National Natural Science Foundation of China (Nos. 22037002, 22007032 and 21977082)the National Mega-project for Innovative Drugs of China (No.2019ZX09721001-004-003)+1 种基金the Chinese Postdoctoral Science Foundation,China (No. 2019M660083)the Shanghai Sailing Program,China (No. 20YF1411200)。
文摘Monoamine oxidase A(MAO-A) is a prominent myocardial source of reactive oxygen species(ROS), and its expression and activity are strongly increased in failing hearts. Therefore, accurate evaluation of MAOA activity in cardiomyocytes is of great importance for understanding its biological functions and early diagnosing the progression of heart failure. However, so far, there is no report on the fluorescent diagnosis of heart failure by a specific probe for MAO-A. In this work, two far-red emissive fluorescent turn-on probes(KXS-M1 and KXS-M2) for the highly selective and sensitive detection of MAO-A were fabricated.Both probes exhibit good response to MAO-A, one of which, KXS-M2, performs better than the other one in terms of a fluorescence increment and sensitivity. Using the pioneering probe KXS-M2, specific fluorescence imaging of MAO-A in glucose-deprived H9c2 cardiac cells, zebrafish and isoprenaline-induced failing heart tissues was achieved, proving that KXS-M2 can serve as a powerful tool for the diagnosis and treatment of heart failure.
