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Trends of Seroprevalence, Epidemiology and Clinical Presentation of HIV in North India: A Tertiary Care Hospital Based Study
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作者 Varun Goel dinesh kumar +3 位作者 Vrushali Patwardhan Veena Balooni Shalini Singhal Sarman Singh 《World Journal of AIDS》 2016年第2期54-58,共5页
Introduction: HIV infection represents a major public health problem for both developing and developed countries as it has grown to global pandemic. Spectrum of clinical presentation of HIV can greatly vary with geopo... Introduction: HIV infection represents a major public health problem for both developing and developed countries as it has grown to global pandemic. Spectrum of clinical presentation of HIV can greatly vary with geopolitical, socioeconomic and cultural environment. Aims: The aim of this study was to estimate the prevalence, socio-demographic conditions, clinical presentations, opportunistic infections and the possible associated risk factors for acquiring HIV infection. Materials and Methods: An observational prospective study was conducted from January 2010 to December 2014 at our centre situated in north India. As per the strategy and policy prescribed by NACO, tests were performed on the serum samples. Results: Out of the total 35369 clients tested for HIV infection, 292 were found to be HIV-1 seropositive. Two HIV-2 cases were found among the studied population. The seroprevalence of HIV was found to be 0.83% (294/35369). Mean age of the study group was 30 ± 5.65 (range: 02 - 80) years. Overall positivity rates among attendees were found to be 0.97% (138/14098), 0.96% (35/3610), 0.82% (7/850), 0.78% (22/2810) and 0.65% (92/14001) in the years 2010, 2011, 2012, 2013 and 2014 respectively. Heterosexual route of transmission was the major route of infection in 78.2 % patients. At the time of presentation, it was observed that Tuberculosis (14.9%) was the most common opportunistic infection. Conclusion: The study shows that there is a decreasing trend in the HIV prevalence in North India. However, the number of HIV cases is still significant and suggests the need for focused prevention efforts in high-risk groups. 展开更多
关键词 AIDS Clinico-Epidemiological Profile HETEROSEXUAL HIV Prevalence India
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舒芬太尼复合罗哌卡因用于硬膜外无痛分娩的临床观察 被引量:20
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作者 dinesh kumar Shrestha 刘萍 张富荣 《昆明医科大学学报》 CAS 2019年第10期109-112,共4页
目的探讨舒芬太尼复合罗哌卡因用于连续硬膜外无痛分娩的效果和安全程度。方法将2018年12月至2019年4月期间昆明医科大学附属延安医院100例在产房正常待产的初产妇随机分为2组(n=50例),实验组常规于L2~3间隙行连续硬膜外穿刺,试验量后... 目的探讨舒芬太尼复合罗哌卡因用于连续硬膜外无痛分娩的效果和安全程度。方法将2018年12月至2019年4月期间昆明医科大学附属延安医院100例在产房正常待产的初产妇随机分为2组(n=50例),实验组常规于L2~3间隙行连续硬膜外穿刺,试验量后头侧置硬膜外管4cm接PCEA泵,协助产妇平卧位后给予舒芬太尼复合罗哌卡因的首剂量和维持剂量,对照组产妇实施常规阴道分娩。观察2组产妇硬膜外自控镇痛效果、产程时间、出血量和新生儿Apgar评分情况,记录是否需要助产和紧急更改剖宫产,是否出现产后出血和胎儿窘迫等不良事件。结果 2组产妇的年龄、身高、体重、孕周值比较差异无统计学意义(P>0.05),实验组产妇分娩镇痛效果的优良率明显高于对照组(P<0.05),2组产妇出血量和新生儿Apgar评分比较差异无统计学意义(P>0.05),实验组产妇第一产程和第二产程时间明显短于对照组(P<0.05),实验组产妇出现需要阴道助产、紧急更改剖宫产和产后出血的比例明显低于对照组(P<0.05)。结论舒芬太尼复合罗哌卡因用于连续硬膜外无痛分娩,镇痛效果显著,能够显著缩短产程时间,提高产妇自然分娩率,降低分娩过程中不良事件的发生率,可以安全有效地应用于临床。 展开更多
关键词 无痛分娩 连续硬膜外麻醉 罗哌卡因 舒芬太尼
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Study of Structural, Optical, Electrical and Dielectric Properties of Gd-Doped ZnO Composites Synthesized by Solid State Reaction Method
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作者 Santosh kumar Kundara Kanhaiya Chawla +3 位作者 dinesh kumar Yadav Chhagan Lal Balram Tripathi Narendra Jakhar 《Open Journal of Composite Materials》 2024年第2期91-108,共18页
In this manuscript, we are reporting structural, bonding, optical, dielectric, and electrical properties of Gd-doped ZnO composite samples (Zn<sub>1</sub><sub>−</sub><sub>x</sub>Gd&... In this manuscript, we are reporting structural, bonding, optical, dielectric, and electrical properties of Gd-doped ZnO composite samples (Zn<sub>1</sub><sub>−</sub><sub>x</sub>Gd<sub>x</sub>O, x = 0, 0.05, 0.10) prepared by solid-state reaction method. XRD spectra confirm the wurtzite hexagonal phase with a grain size distribution of 42 - 47 nm. The FT-IR spectra confirm bonding behavior like Zn-O, O=C=O, and O-H stretching modes. FESEM micrographs show that the grains of crystallites possess nearly spherical morphology. Optical absorption spectra confirm that the optical band gap decreases systematically from 3.19 eV to 3.15 eV for x = 0.0 to x = 0.10 samples. For all samples, PL spectra exhibited near-band emission, blue emission, and green emission peaks. The dielectric constant decreases as the applied frequency increases. Hall effect results show that with increasing doping concentration of Gd, mobility and resistivity increase while bulk concentration decreases. Current-Voltage study shows that current increases when temperature is increased. Rare earth-doped ZnO is potential material used for optoelectronics and spintronics device applications. Properties of Gd-doped ZnO are studied by various research groups, but dielectric studies are limitedly reported. Therefore, the present research work aims to study the change of electrical, optical, and dielectric properties of Gd-doped ZnO for device applications. 展开更多
关键词 ZnO:Gd Composites Optical Band Gap Green Emission Dielectric Constant
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鲍曼不动杆菌分子特征及其在替加环素压力下外排泵表达的变化 被引量:4
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作者 程建军 Kesavan dinesh kumar +5 位作者 阴晴 王慧璇 蔡伟 陈建国 苏兆亮 许化溪 《医学研究生学报》 CAS 北大核心 2020年第1期18-24,共7页
目的鲍曼不动杆菌是医院内感染常见的细菌。文中旨在分析其分子流行特征,检测外排泵和调节蛋白基因的携带率,并研究替加环素对外排泵和调节蛋白基因表达的影响。方法选取2017年5月至2019年3月江苏大学附属医院住院患者183株鲍曼不动杆... 目的鲍曼不动杆菌是医院内感染常见的细菌。文中旨在分析其分子流行特征,检测外排泵和调节蛋白基因的携带率,并研究替加环素对外排泵和调节蛋白基因表达的影响。方法选取2017年5月至2019年3月江苏大学附属医院住院患者183株鲍曼不动杆菌。分为耐药组(一类及以上抗菌药物耐药的菌株,139株),敏感组(药敏试验中的药物均为非耐药的菌株,44株)。应用重复序列PCR作菌株的同源性分析,并以脉冲场凝胶电泳(PFGE)为同源性分析的金标准对部分菌株进行验证和比较。PCR检测耐药相关基因。依据同源性分析、外排泵携带率检测及药敏试验结果,选取6株外排泵基因全携带但耐药表型不同的临床菌株作为实验株,包括敏感株(SAB)、多药耐药株(MDRAB)、广泛耐药株(XDRAB),进行替加环素体外诱导,诱导后的菌株为诱导组,以不含替加环素LB培养的相同菌株为对照组。检测AdeB、TetB等外排泵基因及AdeS、BaeS等调节蛋白的基因表达变化。结果同源性分析显示,183株临床分离株包含有45个克隆群。耐药组AdeB、TetB基因(97.84%、98.56%)检出率均高于敏感组(63.64%、20.45%),CraA、AbeM等基因亦高于敏感组(P<0.05)。替加环素诱导前MDRAB、XDRAB中AdeB(4.17±0.94、6.86±3.23)、TetB(13.06±0.38、13.96±0.63)表达较SAB(0.00±3.70、0.00±0.70)升高(P<0.05),MDRAB菌株中MacB、BaeS表达较SAB菌株亦升高(P<0.05)。MDRAB、XDRAB菌株中AbaQ表达较SAB菌株降低(P<0.05)。诱导组AdeB、TetB在各菌株的表达较对照组明显升高(P<0.01);除SAB1菌株外,AbaQ、AbeM在其余菌株中的表达较对照组明显升高(P<0.05);AdeS在SAB1、SAB2、MDRAB1、XDRAB2菌株中的表达较对照组升高(P<0.05);负调节蛋白SoxR在SAB1、XDRAB1菌株中表达较对照组降低(P<0.05)。结论AdeB、TetB、AbeS、AdeS、BaeS在临床鲍曼不动杆菌多药耐药过程中发挥着重要的作用,其中AdeB和TetB在菌株对替加环素耐药过程� 展开更多
关键词 鲍曼不动杆菌 外排泵基因 同源性分析 脉冲场凝胶电泳 替加环素 耐药
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捕食性瓢虫的体型大小影响其偏好捕食防御性强的猎物吗?(英文) 被引量:2
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作者 Priya PATEL Bhupendra kumar dinesh kumar 《昆虫学报》 CAS CSCD 北大核心 2018年第6期704-711,共8页
【目的】尽管瓢虫表现出种间和种内特异性体型大小的变异,但是瓢虫体型大小的变异是否会改变其选择捕食防御性强(即表现出各种防御反应,包括积极躲避捕食、挣脱捕食者或频繁利用防御性化学物质)的猎物,这一问题尚不清楚。本研究中,我们... 【目的】尽管瓢虫表现出种间和种内特异性体型大小的变异,但是瓢虫体型大小的变异是否会改变其选择捕食防御性强(即表现出各种防御反应,包括积极躲避捕食、挣脱捕食者或频繁利用防御性化学物质)的猎物,这一问题尚不清楚。本研究中,我们假设不论体型大小,捕食性瓢虫七星瓢虫Coccinella septempunctata(L.)和六斑月瓢虫Menochilus sexmaculatus(Fab.)都将增加其对猎物萝卜蚜Lipaphis erysimi(Kaltenbach)的捕食,只要猎物处于防御性强的龄期,原因是处于防御性强的龄期的猎物体型大且更有活力。【方法】我们将萝卜蚜4龄若虫期作为防御性强的龄期,将2龄若虫期作为防御性弱的龄期,评价七星瓢虫和六斑月瓢虫这两种瓢虫的大型和小型变异体对萝卜蚜的偏好性。【结果】结果表明,六斑月瓢虫的大型和小型雌性变异体更多地捕食防御性弱的萝卜蚜,而七星瓢虫的大型和小型雌性变异体分别对防御性强和防御性弱的萝卜蚜捕食较多。在混合饲料上,七星瓢虫雌性变异体对两类蚜虫的捕食百分比相似,而六斑月瓢虫变异体对防御性弱的萝卜蚜的捕食百分比较高。尽管随着七星瓢虫雌虫体型的增大而对两类猎物的捕食增多,但随着六斑月瓢虫雌虫体型的增大,只对防御性强的蚜虫的捕食量增加。【结论】本研究结果与我们的假设相反,并证明了:(1)小型瓢虫更多地只是捕食防御性弱的猎物,而大型瓢虫选择性地捕食防御性强的猎物;(2)在瓢虫种内和种间,随着捕食者体型的增大,对防御性强的猎物的偏好性增加。可将这些结果用于瓢虫的室内大规模饲养,以提高对萝卜蚜的生物防治。 展开更多
关键词 七星瓢虫 六斑月瓢虫 萝卜蚜 瓢虫 蚜虫 生物防治 警戒信号
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A Decisive Study on Dielectric Response of Bi2O3/Polystyrene &Bi2O3/PVDF Composite as Flexible Electrodes for Energy Storage
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作者 dinesh kumar Yadav Anju Yadav +2 位作者 Sushil kumar Jain Narendra Jakhar Balram Tripathi 《Open Journal of Composite Materials》 CAS 2023年第1期1-11,共11页
In this manuscript a comparative study on Bi<sub>2</sub>O<sub>3</sub>/polystyrene and Bi<sub>2</sub>O<sub>3</sub>/PVDF composites has been executed via analysis of struc... In this manuscript a comparative study on Bi<sub>2</sub>O<sub>3</sub>/polystyrene and Bi<sub>2</sub>O<sub>3</sub>/PVDF composites has been executed via analysis of structural, bonding, surface morphology and dielectric response of composites for energy storage. The composites have been synthesized using solution cast method by varying concentrations of Bi<sub>2</sub>O<sub>3</sub> (BO = 1 - 5 mw%) into polystyrene (PS) and polyvinylidene fluoride (PVDF) polymers respectively. X-ray diffraction confirms the generation of crystallinity, Fourier transform infrared (FT-IR) spectroscopy confirms bonding behavior and scanning electron microscopy (SEM) confirms uniform distribution of Bi<sub>2</sub>O<sub>3</sub> (BO) in PS and PVDF polymers. Impedance spectroscopy has been employed for determination of dielectric response of the fabricated composites. The dielectric constant has been found to be increased as 1.4 times of pristine PS to BO<sub>5%</sub>PS<sub>95%</sub> composites and 1.8 times of pristine PVDF to BO<sub>5%</sub>PVDF<sub>95%</sub> composites respectively. These high dielectric composite electrodes are useful for flexible energy storage devices. 展开更多
关键词 Bismuth Oxide (Bi2O3) Polymer Composites Surface Morphology Dielectric Constant Energy Storage
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防御性被捕食者可利用量的波动可调节六斑月瓢虫的功能反应曲线(英文) 被引量:1
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作者 Priya PATEL Bhupendra kumar dinesh kumar 《昆虫学报》 CAS CSCD 北大核心 2017年第9期1031-1040,共10页
【目的】本研究评价了在被捕食者波动条件下,在花生蚜Aphis craccivora Koch和萝卜蚜Lipaphis erysimi(Kaltenbach)上六斑月瓢虫Menochilus sexmaculatus(Fabricius)4龄幼虫的功能反应。萝卜蚜是一种含烯丙基异硫氰酸酯的防御性被捕食... 【目的】本研究评价了在被捕食者波动条件下,在花生蚜Aphis craccivora Koch和萝卜蚜Lipaphis erysimi(Kaltenbach)上六斑月瓢虫Menochilus sexmaculatus(Fabricius)4龄幼虫的功能反应。萝卜蚜是一种含烯丙基异硫氰酸酯的防御性被捕食者。我们推测,在试验的被捕食者条件下,在最适量被捕食者或丰富的被捕食者条件下饲养的幼虫比在缺乏被捕食者条件下饲养的幼虫表现出更好的功能反应。而在萝卜蚜上六斑月瓢虫的功能反应曲线的斜率比在花生蚜上要低。【方法】将六斑月瓢虫4龄幼虫从饲养条件(缺乏被捕食者/最适乏被捕食者/丰富的被捕食者)突然转移到不同的试验条件(极为缺乏被捕食者/缺乏被捕食者/次适量被捕食者/最适量被捕食者/丰富的被捕食者)下,外推出其功能曲线。【结果】尽管被捕食资源发生了改变,但是六斑月瓢虫4龄幼虫在花生蚜上表现出Ⅱ型反应,而在萝卜蚜上表现出改变的Ⅱ型反应。此外,在5个试验条件下,不管是哪种被捕食者,在最适量被捕食者/丰富的被捕食者条件下饲养的4龄幼虫均表现出正常的捕食率。但是在缺乏被捕食者条件下饲养的4龄幼虫突然转变到最适量/丰富的被捕食者实验条件下时,它们通过取食比平时更多的被捕食者的生物量来补偿食物的短缺。不过,它们在丰富的被捕食者条件下的取食量最高,在极为缺乏被捕食者的试验条件下的取食量最低。而且,在花生蚜上记录到较高的攻击率和较低的猎物处理时间。【结论】结果说明,萝卜蚜可利用量的波动可改变六斑月瓢虫幼虫的功能反应。然而,对于六斑月瓢虫而言,花生蚜比萝卜蚜是更为合适的被捕食者。 展开更多
关键词 瓢虫 蚜虫 生物防治 功能反应 资源波动
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Possible Mechanical Transmission of SARS-CoV-2 Causing COVID-19 by Insects: Infection, Prevention, Implications, and Control
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作者 Mubarak Ismail Abhishek kumar Verma +4 位作者 Adamu Abdulkadir Avinash kumar dinesh kumar Dhawan Kinjal Bolya Mayadhar Barik 《Open Journal of Medical Microbiology》 2020年第2期89-101,共13页
<span style="font-family:Verdana;">The new coronavirus called SARS-CoV-2 is a new type of virus named as COVID-19. Although, </span><span style="font-family:Verdana;">it has </... <span style="font-family:Verdana;">The new coronavirus called SARS-CoV-2 is a new type of virus named as COVID-19. Although, </span><span style="font-family:Verdana;">it has </span><span style="font-family:Verdana;">few similarities with pandemic flu viruses</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> the respiratory system and immune system are damaged through the viruses infected the population wh</span><span style="font-family:Verdana;">o</span><span style="font-family:Verdana;"> has weakened immunity. SARS-CoV-2 spread</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> when people don’t have the sign and symptoms. This virus COVID-19 appears to spread more easily than the flu, and asymptomatic transmission may account for a greater proportion of COVID-19’s spreader over the World. In inundation of the current understanding</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> the roles of insect vectors are helping in the transmission of viral pathogens as well and the possible roles of some newly joined insects in the mechanical transmission of COVID-19.</span><span style="font-family:Verdana;"> We also specifically provide the prevention and control methods related to contamination, disease burden, risk pattern in the family, near and dear to maintain the precision of social distancing and development of the immune system to fight against SARS-CoV-2. 展开更多
关键词 SARS-CoV-2 INSECTS PREVENTION Control Mechanical Transmission
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The Development of Protein Microarrays and Their Applications in DNA-Protein and Protein-Protein Interaction Analyses of Arabidopsis Transcription Factors 被引量:7
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作者 Wei Gong Kun He +5 位作者 Mike Covington S. R dinesh-kumar Michael Snyder Stacey L. Harmer Yu-Xian Zhu Xing Wang Denga 《Molecular Plant》 SCIE CAS CSCD 北大核心 2008年第1期27-41,共15页
We used our collection of Arabidopsis transcription factor (TF) ORFeome clones to construct protein microarrays containing as many as 802 TF proteins. These protein microarrays were used for both protein-DNA and pro... We used our collection of Arabidopsis transcription factor (TF) ORFeome clones to construct protein microarrays containing as many as 802 TF proteins. These protein microarrays were used for both protein-DNA and proteinprotein interaction analyses. For protein-DNA interaction studies, we examined AP2/ERF family TFs and their cognate cis-elements. By careful comparison of the DNA-binding specificity of 13 TFs on the protein microarray with previous non-microarray data, we showed that protein microarrays provide an efficient and high throughput tool for genome-wide analysis of TF-DNA interactions. This microarray protein-DNA interaction analysis allowed us to derive a comprehensive view of DNA-binding profiles of AP2/ERF family proteins in Arabidopsis. It also revealed four TFs that bound the EE (evening element) and had the expected phased gene expression under clock-regulation, thus providing a basis for further functional analysis of their roles in clock regulation of gene expression. We also developed procedures for detecting protein interactions using this TF protein microarray and discovered four novel partners that interact with HY5, which can be validated by yeast two-hybrid assays. Thus, plant TF protein microarrays offer an attractive high-throughput alternative to traditional techniques for TF functional characterization on a global scale. 展开更多
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Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation 被引量:6
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作者 Xiaohong Zhu Sivakumar Pattathil +4 位作者 Koushik Mazumder Amanda Brehm Michael G. Hahn S.P. dinesh-kumar Chandrashekhar P. Joshi 《Molecular Plant》 SCIE CAS CSCD 2010年第5期818-833,共16页
Virus-induced gene silencing (VIGS) is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus (TRV)-based VlGS system... Virus-induced gene silencing (VIGS) is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus (TRV)-based VlGS system to study functions of genes involved in either primary or secondary cell wall formation in Nicotiana benthamiana plants. A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective. 展开更多
关键词 Plant cell wall VIGS CELLULOSE XYLAN LIGNIN Nicotiana.
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A Plant Immune Receptor Adopts a Two-Step Recognition Mechanism to Enhance Viral Effector Perception 被引量:6
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作者 Jia Li Haining Huang +4 位作者 Min Zhu Shen Huang Wenhua Zhang Savithramma P. dinesh-kumar Xiaorong Tao 《Molecular Plant》 SCIE CAS CSCD 2019年第2期248-262,共15页
Plant intracellular nucleotide binding leucine-rich repeat (NLR) immune receptors play critical roles in pathoge n surveillance. Most plant NLRs characterized so far were found to use a single domain/sensor to recogni... Plant intracellular nucleotide binding leucine-rich repeat (NLR) immune receptors play critical roles in pathoge n surveillance. Most plant NLRs characterized so far were found to use a single domain/sensor to recognize pathogen effectors. Here we report that the Sw-5b NLR immune receptor uses two distinct domains to detect the viral movement protein NSm encoded by tospovirus. In addition to its leucine-rich repeat (LRR) domain that has been previously reported, the N-terminal Solanaceae domain (SD) of Sw- 5b also interacts with NSm and a conserved 21-amino-acid region of NSm (NSm^21). The specific interaction between Sw-5b SD and NSm is required for releasing the inhibitory effect of coiled-coil domain on the NBARC- LRR region. Furthermore, we found that the binding of NSm affects the nucleotide binding activity of the NB-ARC-LRR in vitro, while Sw-5b NB-ARC-LRR is activated only when NSm and NSm^21 levels are high. Interestingly, Sw-5b SD could significantly enhanee the ability of the NB-ARC-LRR to detect low levels of NSm effector and facilitate its activation and induction of defense response. An Sw-5b SD mutant that is disrupted in NSm recognition failed to enhance the ability of the NB-ARC-LRR to sense low levels of NSm and NSm^21 . Taken together, our results suggest that Sw-5b SD functions as an extra sensor and the NB-ARC-LRR as an activator, and that Sw-5b NLR adopts a two-step recog nition mechanism to enhance viral effector perception. 展开更多
关键词 plant INNATE immunity nucleotide binding leucine-rich repeat (NLR) immune receptors SOLANACEAE domain (SD) pathogen PERCEPTION TWO-STEP recognition defense response
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AtGGM2014, an Arabidopsis gene co-expression network for functional studies 被引量:3
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作者 MA ShiSong BOHNERT Hans J dinesh-kumar Savithramma P 《Science China(Life Sciences)》 SCIE CAS CSCD 2015年第3期276-286,共11页
Gene co-expression networks provide an important tool for systems biology studies. Using microarray data from the Array Express database, we constructed an Arabidopsis gene co-expression network, termed At GGM2014, ba... Gene co-expression networks provide an important tool for systems biology studies. Using microarray data from the Array Express database, we constructed an Arabidopsis gene co-expression network, termed At GGM2014, based on the graphical Gaussian model, which contains 102,644 co-expression gene pairs among 18,068 genes. The network was grouped into 622 gene co-expression modules. These modules function in diverse house-keeping, cell cycle, development, hormone response, metabolism, and stress response pathways. We developed a tool to facilitate easy visualization of the expression patterns of these modules either in a tissue context or their regulation under different treatment conditions. The results indicate that at least six modules with tissue-specific expression pattern failed to record modular regulation under various stress conditions. This discrepancy could be best explained by the fact that experiments to study plant stress responses focused mainly on leaves and less on roots, and thus failed to recover specific regulation pattern in other tissues. Overall, the modular structures revealed by our network provide extensive information to generate testable hypotheses about diverse plant signaling pathways. At GGM2014 offers a constructive tool for plant systems biology studies. 展开更多
关键词 ARABIDOPSIS gene co-expression network graphical Gaussian model plant development stress response hormone response
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High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals 被引量:2
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作者 Jubin Wang Qingling Zhang +12 位作者 Jeffrey Tung Xi Zhang Dan Liu Yingtian Deng Zhendong Tian Huilan Chen Taotao Wang Weixiao Yin Bo Lij Zhibing Lai Savithramma P.dinesh-kumar Barbara Baker Feng Li 《Molecular Plant》 SCIE CSCD 2024年第3期423-437,共15页
Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology research.However,genomic studies of these species have lagged.Here we report the chromosome-level reference genome assemblies for N.b... Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology research.However,genomic studies of these species have lagged.Here we report the chromosome-level reference genome assemblies for N.benthamiana and N.tabacum with an estimated 99.5%and 99.8%completeness,respec-tively.Sensitive transcription start and termination site sequencing methods were developed and used for accurate gene annotation in N.tabacum.Comparative analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each species.Interestingly,theantiviral silencinggenesRDR1,RDR6,DCL2,DCL3,andAGO2were lost from one or both subgenomes in N.benthamiana,while both homeologs were kept in N.tabacum.Furthermore,the N.benthamiana genome encodes fewer immune receptors and signaling components than that of N.tabacum.These find-ings uncover possible reasons underlying the hypersusceptible nature of N.benthamiana.We developed the user-friendly Nicomics(http:/lifenglab.hzau.edu.cn/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses. 展开更多
关键词 Nicotiana tabacum Nicotiana benthamiana GENOMICS evolution disease resistance NLR
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Proxitome profiling reveals a conserved SGT1-NSL1 signaling module that activates NLR-mediated immunity 被引量:1
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作者 Dingliang Zhang Xinxin Yang +13 位作者 Zhiyan Wen Zhen Li Xinyu Zhang Chenchen Zhong Jiajie She Qianshen Zhang He Zhang Wenli Li Xiaoyun Zhao Mingliang Xu Zhen Su Dawei Li Savithramma P.dinesh-kumar Yongliang Zhang 《Molecular Plant》 SCIE CSCD 2024年第9期1369-1391,共23页
Suppressor of G2 allele of skp1(SGT1)is a highly conserved eukaryotic protein that plays a vital role in growth,development,and immunity in both animals and plants.Although some SGT1 interactors have been identified,t... Suppressor of G2 allele of skp1(SGT1)is a highly conserved eukaryotic protein that plays a vital role in growth,development,and immunity in both animals and plants.Although some SGT1 interactors have been identified,the molecular regulatory network of SGT1 remains unclear.SGT1 serves as a co-chaperone to stabilize protein complexes such as the nucleotide-binding leucine-rich repeat(NLR)class of immune receptors,thereby positively regulating plant immunity.SGT1 has also been found to be asso-ciated with the SKP1-Cullin-F-box(SCF)E3 ubiquitin ligase complex.However,whether SGT1 targets im-mune repressors to coordinate plant immune activation remains elusive.In this study,we constructed a toolbox for TurbolD-and split-TurbolD-based proximity labeling(PL)assays in Nicotiana benthamiana and used the PL toolbox to explore the SGT1 interactome during pre-and post-immune activation.The comprehensive SGT1 interactome network we identified highlights a dynamic shift from proteins associ-ated with plant development to those linked with plant immune responses.We found that SGT1 interacts with Necrotic Spotted Lesion1(NSL1),which negatively regulates salicylic acid-mediated defenseby inter-fering with the nucleocytoplasmic trafficking of non-expressor of pathogenesis-related genes 1(NPR1)during N NLR-mediated response to tobacco mosaic virus.SGT1 promotes the SCF-dependent degrada-tion of NSL1 to facilitate immune activation,while salicylate-induced protein kinase-mediated phosphory-lation of SGT1further potentiates this process.Besides NNLR,NSL1also functions in several other NLR-mediated immunity.Collectively,our study unveils the regulatory landscape of SGT1 and reveals a novel SGT1-NSL1 signaling module that orchestrates plant innate immunity. 展开更多
关键词 suppressor of G2 allele of skp1 SGT1 proximity labeling N NLR immune receptor Necrotic Spotted Lesion 1 NSL1 salicylic acid UBIQUITINATION
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Proximity labeling: an emerging tool for probing in planta molecular interactions 被引量:3
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作者 Xinxin Yang Zhiyan Wen +5 位作者 Dingliang Zhang Zhen Li Dawei Li Ugrappa Nagalakshmi Savithramma P.dinesh-kumar Yongliang Zhang 《Plant Communications》 2021年第2期1-15,共15页
Protein–protein interaction(PPI)networks are key to nearly all aspects of cellular activity.Therefore,the identification of PPIs is important for understanding a specific biological process in an organism.Compared wi... Protein–protein interaction(PPI)networks are key to nearly all aspects of cellular activity.Therefore,the identification of PPIs is important for understanding a specific biological process in an organism.Compared with conventional methods for probing PPIs,the recently described proximity labeling(PL)approach combined with mass spectrometry(MS)-based quantitative proteomics hasemerged as apowerful approach for characterizing PPIs.However,the application of PL in planta remains in its infancy.Here,we summarize recent progress in PL and its potential utilization in plant biology.We specifically summarize advances in PL,including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems. 展开更多
关键词 protein interactions proximity labeling biotin ligase plant membrane contact sites ORGANELLES
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