The dating of the uplift onset of the Huangshan pluton in the southern part of Anhui Province associated with the collision between paleo-Pacific Block and the Eurasian Block is a fundamental issue to better understan...The dating of the uplift onset of the Huangshan pluton in the southern part of Anhui Province associated with the collision between paleo-Pacific Block and the Eurasian Block is a fundamental issue to better understand the uplift mechanisms and the regional tectonic evolution. Х^2 values of seven zircon fission track （ZFF） samples collected from the south part of the Huangshan pluton were all 〈5%. Based on the grain ages of four typical ZFr samples, three thermal overprints ranging in 113-123, 72-95 and 49-66 Ma were distinguished respectively using the binomial peak-fitting method in accordance with the tectonic thermal events after south China shifted into circum-Pacific tectonic region. Apatite fission track （AFT） study of the Huangshan pluton shows ages lie between 15±3 and 56±6 Ma with all Х^2 values 〉5%, and all are significantly younger than their host rock formation ages, indicating that the samples have experienced post-formation thermal history. Based on the AFT resuits and topography characteristics in the Huangshan pluton, three zones with different denudation intensities caused by the differential uplift of the fault blocks were recognized in this paper, indicating that denudation was driven by the increase in elevation significantly. Modeling of the AFT data shows that the earlier cooling phase took place in the early Cenozoic and involved 3.3, 3.8 and 6.0℃/Ma of cooling rates equivalent to the exhumation rates of 90, 104 and 167 m/Ma in different fault blocks. The last phase of cooling took place from -10 Ma with average cooling rate of -5.6℃/Ma, equivalent to average denudation rates of -156 m/Ma. The fission track results imply that the regional compression field plays an important role for the differential exhumation between individual fault blocks of the Huangshan pluton.展开更多
Pathological vascular remodeling is a hallmark of various vascular diseases.Previous research has established the significance of andrographolide in maintaining gastric vascular homeostasis and its pivotal role in mod...Pathological vascular remodeling is a hallmark of various vascular diseases.Previous research has established the significance of andrographolide in maintaining gastric vascular homeostasis and its pivotal role in modulating endothelial barrier dysfunction,which leads to pathological vascular remodeling.Potassium dehydroandrographolide succinate(PDA),a derivative of andrographolide,has been clinically utilized in the treatment of inflammatory diseases precipitated by viral infections.This study investigates the potential of PDA in regulating pathological vascular remodeling.The effect of PDA on vascular remodeling was assessed through the complete ligation of the carotid artery in C57BL/6 mice.Experimental approaches,including rat aortic primary smooth muscle cell culture,flow cytometry,bromodeoxyuridine(BrdU)incorporation assay,Boyden chamber cell migration assay,spheroid sprouting assay,and Matrigel-based tube formation assay,were employed to evaluate the influence of PDA on the proliferation and motility of smooth muscle cells(SMCs).Molecular docking simulations and co-immunoprecipitation assays were conducted to examine protein interactions.The results revealed that PDA exacerbates vascular injury-induced pathological remodeling,as evidenced by enhanced neointima formation.PDA treatment significantly increased the proliferation and migration of SMCs.Further mechanistic studies disclosed that PDA upregulated myeloid differentiation factor 88(MyD88)expression in SMCs and interacted with T-cadherin(CDH13).This interaction augmented proliferation,migration,and extracellular matrix deposition,culminating in pathological vascular remodeling.Our findings underscore the critical role of PDA in the regulation of pathological vascular remodeling,mediated through the MyD88/CDH13 signaling pathway.展开更多
Li-rich cathode materials have been considered as promising candidates for high-energy lithium ion batteries （LIBs）. In this study, we report a new series of Li-rich materials （Li[Li1/B-2x/BMn2/3-x/3Nix]O2 （0.09 ...Li-rich cathode materials have been considered as promising candidates for high-energy lithium ion batteries （LIBs）. In this study, we report a new series of Li-rich materials （Li[Li1/B-2x/BMn2/3-x/3Nix]O2 （0.09 ≤x≤ 0.2）） doped with small amounts of Ni as cathode materials in LIBs, which exhibited unusual phenomenon of capacity increase up to tens of cycles due to the continuous activation of the Li2MnO3 phase. Both experimental and computational results indicate that unlike commonly studied Ni-doped Li-rich cathode materials, smaller amounts of Ni doping can promote the stepwise Li2MnO3 activation to obtain increased specific capacity and better cycling capability. In contrast, excessive Ni will over-activate the Li2MnO3 and result in a large capacity loss in the first cycle. The Lil.25Mn0.625Ni0.12sO2 material with an optimized content of Ni delivered a superior high capacity of -280 mAh.g-1 and good cycling stability at room temperature.展开更多
Mutations in calcium-dependent papain-like protease CALPAIN3(CAPN3)cause Limb-Girdle Muscular Dystrophy Recessive Type 1(LGMDR1),the most common limb-girdle muscular dystrophy in humans.In addition to progressive musc...Mutations in calcium-dependent papain-like protease CALPAIN3(CAPN3)cause Limb-Girdle Muscular Dystrophy Recessive Type 1(LGMDR1),the most common limb-girdle muscular dystrophy in humans.In addition to progressive muscle weakness,persistent inflammatory infiltration is also a feature of LGMDR1.Despite the underlying mechanism remaining poorly understood,we consider that it may relate to the newly defined role of CAPN3/Capn3b in the nucleolus.Here,we report that the loss of function of zebrafish capn3b,the counterpart of human CAPN3,induces an autoimmune response akin to that in LGMDR1 patients.capn3b mutant larvae are more susceptible to Listeria monocytogenes injection,characterized by recruiting more macrophages.Under germ-free conditions,transcriptome analysis of the capn3b mutant muscle reveals a significant upregulation of the chemokine-production-related genes.Coincidently,more neutrophils are recruited to the injury site imposed by either muscle stabbing or tail fin amputation.Nucleolar proteomic analysis and enzymatic assays reveal NKAP,an activating factor of the NF-k B pathway,to be a target of CAPN3.We conclude that the accumulation of Nkap and other factors in the capn3b mutant may be involved in the over-activation of innate immunity.Our studies indicate that the zebrafish capn3b mutant is a powerful model for studying the immunity-related progression of human LGMDR1.展开更多
Li4Ti5012 (LTO) with rich R-TiO2 (17.06, 23.69, and 34.42 wt%), namely, R-TiO2@Li4Ti5O12 composites, were synthesized using the hydrothermal method and tetrabutyl titanate (TBT) as the precursor. Rietveld refinement o...Li4Ti5012 (LTO) with rich R-TiO2 (17.06, 23.69, and 34.42 wt%), namely, R-TiO2@Li4Ti5O12 composites, were synthesized using the hydrothermal method and tetrabutyl titanate (TBT) as the precursor. Rietveld refinement of X-ray diffraction (XRD) results show that the proportion of Li occupying 16d sites is extraordinary low and the lattice constants of LTO and R-TiO2 change with the ritanium dioxide content. EIS measurements showed that with in creasing R-TiO2 content, both its charge transfer impedance (Rct) and lithium ion diffusion coefficient (DLi) decreased. The changes of Rct and DLi caused by the increase of titanium dioxide content have synergic-antagonistic effects on the rate and cycle properties of Li4Ti5012. The rate performance is positively related to DLi, while the cycle property is negatively correlated with Rct, indicati ng that the rate performs nee is mainly related to DLi, while Rct more significantly affects the cycle performance. LTO-RT-17.06% exhibited excellent rate properties, especially under a high current density (5.0 C, 132.5 mAh/g) and LTO-RT-34.42% showed superior long-term cycle performance (0.012% capacity loss per cycle) compared to that of LTO-RT-17.06% and LTO-RT-23.69%.展开更多
Microlenses or arrays are key elements in many applications.However,their construction methods involve multiple fabrication processes,thereby increasing the complexity and cost of fabrication.In this study,we demonstr...Microlenses or arrays are key elements in many applications.However,their construction methods involve multiple fabrication processes,thereby increasing the complexity and cost of fabrication.In this study,we demonstrate an optically anisotropic,electrically tunable liquid crystal(LC)microlens array using a simple,one-step fabrication method.The microlens array is formed via photopolymerization-induced phase separation inside a polymer/LC composite.It possesses both polarization-dependent and electrically tunable focusing and imaging properties.Without applying voltage,the microlens array has a natural focal length of 8 mm,which is a result of its inherent gradient refractive index profile.Upon applying voltage above the threshold,the LC molecules reorient along the electric field direction and the focal length of the microlens array gradually increases.Based on its superior properties,the microlens array is further used for integral imaging applications,demonstrating electrically tunable central depth plane.Such LC microlens arrays could find numerous potential applications owing to their advantageous features of being flat,ultra-thin,and tunable,including 3D displays,optical interconnects,and more.展开更多
Solid-state lithium batteries(SSLBs)solve safety issues and are potentially energy-dense alternatives to next-generation energy storage systems.Battery green recycling routes are responsible for the widespread use of ...Solid-state lithium batteries(SSLBs)solve safety issues and are potentially energy-dense alternatives to next-generation energy storage systems.Battery green recycling routes are responsible for the widespread use of SSLBs due to minimizing environmental contamination,reducing production costs,and providing a sustainable solution for resources,e.g.,saving rare earth elements(La,Ta,etc.).Herein,a solid-state recycling strategy is proposed to achieve green recycling of the crucial component solidstate electrolytes(SSEs)in spent SSLBs.The short-circuited garnet Li_(6.5)La_(3)Zr_(1.5)Ta_(0.5)O_(12)(LLZTO)is broken into fine particles and mixed with fresh particles to improve sintering activity and achieve high packing density.The continuous Li absorption process promotes sufficient grain fusion and guarantees the transformation from tetragonal phase to pure cubic phase for high-performance recycled LLZTO.The Li-ion conductivity reaches 5.80×10^(-4)S cm-1with a relative density of 95.9%.Symmetric Li cell with asrecycled LLZTO shows long-term cycling stability for 700 h at 0.3 mA cm^(-2)without any voltage hysteresis.Full cell exhibits an excellent cycling performance with a discharge capacity of 141.5 mA h g^(-1)and a capacity retention of 92.1%after 400 cycles(0.2C).This work develops an environmentally friendly and economically controllable strategy to recycle SSE from spent SSLBs,guiding future directions of SSLBs large-scale industrial application and green recycling study.展开更多
Recovery of liver mass to a healthy liver donor by compensatory regeneration after partial hepatectomy(PH)is a prerequisite for liver transplantation.Synchronized cell cycle reentry of the existing hepatocytes after P...Recovery of liver mass to a healthy liver donor by compensatory regeneration after partial hepatectomy(PH)is a prerequisite for liver transplantation.Synchronized cell cycle reentry of the existing hepatocytes after PH is seemingly a hallmark of liver compensatory regeneration.Although the molecular control of the PH-triggered cell cycle reentry has been extensively studied,little is known about how the synchronization is achieved after PH.The nucleolus-localized protein cleavage complex formed by the nucleolar protein Digestive-organ expansion factor(Def)and cysteine proteinase Calpain 3(Capn3)has been implicated to control wounding healing during liver regeneration through selectively cleaving the tumor suppressor p53 in the nucleolus.However,whether the Def-Capn3 complex participates in regulating the synchronization of cell cycle reentry after PH is unknown.In this report,we generated a zebrafish capn3b null mutant(capn3b^(Δ19Δ14)).The homozygous mutant was viable and fertile,but suffered from a delayed liver regeneration after PH.Delayed liver regeneration in capn3b^(Δ19Δ14)was due to disruption of synchronized cell proliferation after PH.Mass spectrometry(MS)analysis of nuclear proteins revealed that a number of negative regulators of cell cycle are accumulated in the capn3b^(Δ19Δ14)liver after PH.Moreover,we demonstrated that Check-point kinase 1(Chk1)and Wee1,two key negative regulators of G2 to M transition,are substrates of Capn3.We also demonstrated that Chk1 and Wee1 were abnormally accumulated in the nucleoli of amputated capn3bΔ19Δ14 liver.In conclusion,our findings suggest that the nucleolar-localized Def-Capn3 complex acts as a novel regulatory pathway for the synchronization of cell cycle reentry,at least partially,through inactivating Chk1 and Wee1 during liver regeneration after PH.展开更多
The nucleolus,as the‘nucleus of the nucleus’,is a prominent subcellular organelle in a eukaryocyte.The nucleolus serves as the centre for ribosome biogenesis,as well as an important site for cell-cycle regulation,ce...The nucleolus,as the‘nucleus of the nucleus’,is a prominent subcellular organelle in a eukaryocyte.The nucleolus serves as the centre for ribosome biogenesis,as well as an important site for cell-cycle regulation,cellular senescence,and stress response.The protein composition of the nucleolus changes dynamically through protein turnover to meet the needs of cellular activities or stress responses.Recent studies have identified a nucleolus-localized protein degradation pathway in zebrafish and humans,namely the Def-CAPN3 pathway,which is essential to ribosome production and cell-cycle progression,by controlling the turnover of multiple substrates(e.g.,ribosomal small-subunit[SSU]processome component Mpp10,transcription factor p53,check-point proteins Chk1 and Wee1).This pathway relies on the Ca2þ-dependent cysteine proteinase CAPN3 and is independent of the ubiquitin-mediated proteasome pathway.CAPN3 is recruited by nucleolar protein Def from cytoplasm to nucleolus,where it proteolyzes its substrates which harbor a CAPN3 recognition-motif.Def depletion leads to the exclusion of CAPN3 and accumulation of p53,Wee1,Chk1,and Mpp10 in the nucleolus that result in cell-cycle arrest and rRNA processing abnormality.Here,we summarize the discovery of the Def-CAPN3 pathway and propose its biological role in cell-cycle control and ribosome biogenesis.展开更多
Objective： To establish an experimental model of urethral stricture in rabbits. Methods： A total of 21 adult male New Zealand rabbits were included into group. After intravenous anesthesia, urethroscopy was performe...Objective： To establish an experimental model of urethral stricture in rabbits. Methods： A total of 21 adult male New Zealand rabbits were included into group. After intravenous anesthesia, urethroscopy was performed with a pediatric resectoscope （F13）. Fifteen animals were randomly selected as the study group. A lcm-long circumferential electrocoagulation of the bulbar urethra was performed to these animals until ulceration of the mucosa. The remaining 6 animals underwent video urethroscopy without electrocoagulation, serving as controls. On the 30th day, retrograde urethrogram and urethroscopy were performed to evaluate urethral stricture formation, histological examinations （HE and Sirius Red staining） were done to assess urethral pathological change. Results： Two rabbits in study group died and no death occurred in controls. Based on urethrogram and urethroscopy, no rabbits in control group developed urethral stricture, while significant stricture formation was observed in every case of the study group. Histological examination showed a normal urethra in control cases, while at stricture site of the study group extensive fibrosis of muscle and submucous tis High collagen expression in fibrosis tissue was assayed sue by was observed with a large number of fibroblasts infiltration Sirius Red staining. Conclusion： A lcm-long endoscopic electrocoagulation can successfully induce urethral stricture formation in rabbit models. This method offers an ideal animal model for the fundamental and clinical study of urethral stricture展开更多
This paper presents partially asynchronous parallel simulation of continuous-system (PAPSoCS) and some approaches to the issues of its implementation on a multicomputer system. To guarantee the simulation results cor...This paper presents partially asynchronous parallel simulation of continuous-system (PAPSoCS) and some approaches to the issues of its implementation on a multicomputer system. To guarantee the simulation results correct and speedup the simulation, the scheme for efficient PAPSoCS is proposed and the virtual topology star is constructed to match the path of message passing for solving algorithm-architecture adequation problem. Under the circumstances that messages frequently passed inter-processor are much shorter, typically within several 4 bytes, asynchronous communication mode is employed to reduce the communication ratio. Experiment results show that asynchronous parallel simulation has much higher efficiency than its synchronous counterpart.展开更多
In this paper, a homogenous parallel simulation system is presented in detail for continuous--system simulation. The system is collstructed by a host computer and I I transputers connected into a topologyof 'Super...In this paper, a homogenous parallel simulation system is presented in detail for continuous--system simulation. The system is collstructed by a host computer and I I transputers connected into a topologyof 'Super--Node' which is very suitable for simulation of stiff systems. An automatic software interface runin the host is developed to partition simulation model, either equations or block diagrams, into several equitable segments and then pack them into parallel simulation program to be executed in the parallel system.This interface frees simulation users from parallel programming to focus on their simulation experiments.展开更多
High performance of parallel computing on a message-passing multicomputer System relies on the balance of the workloads located on the processing elements of the System and the minimum communication ovcrheads among th...High performance of parallel computing on a message-passing multicomputer System relies on the balance of the workloads located on the processing elements of the System and the minimum communication ovcrheads among them. Mapping is the technology to partition the problem domain wellbalanced into multiple distinct execution tasks based on some measures. In mapping, a good objective function is the criterion to guarantce the distinct execution tasks equitable. In this paper, we evaluate five categories of those existed objective functions with three different problem subjects using experiments and find an objective function is much suitable for all kinds of problems.展开更多
In today’s era,multidisciplinary integration is the development trend of education,which breaks the field of a single discipline and lays the foundation for the development of education.Objectively speaking,with the ...In today’s era,multidisciplinary integration is the development trend of education,which breaks the field of a single discipline and lays the foundation for the development of education.Objectively speaking,with the integration of disciplines,a new interdisciplinary is formed.This new discipline is comprehensive and systematic;in addition,it integrates knowledge,technology,and methods.It is the result of the collision of different disciplines.As an applied course,Management Science and Engineering integrates multidisciplinary knowledge,such as science and technology,information science,and mathematics.Its main purpose is to solve various problems in social and economic development.It has a long-term impact on the development of contemporary multidisciplinary education.In the new era,in order to meet the requirements of social and economic development,the teaching of Management Science and Engineering should be based on the perspective of multidisciplinary integration,combined with the current characteristics and training mode of the discipline,explore new talent training paths,and then drive the development of the whole industry.展开更多
Background: Hepatocellular adenoma is a rare liver tumor that may require surgical treatment in cases of hemorrhage or suspicion of malignant lesions. Aim: To analyze data from patients who underwent hepatectomy for h...Background: Hepatocellular adenoma is a rare liver tumor that may require surgical treatment in cases of hemorrhage or suspicion of malignant lesions. Aim: To analyze data from patients who underwent hepatectomy for hepatocellular adenoma (HCA) in Rio de Janeiro, Brazil. Methods: From January 2005 to March 2019, sixty-nine patients with HCA underwent hepatectomy at centers in Rio de Janeiro. They were included in the analysis patients undergoing hepatectomy with pathological diagnosis of hepatocellular adenoma and excluded patients with hepatectomy with anatomopathological diagnosis other than hepatocellular adenoma, mainly nodular focal hyperplasia. Data related to patients, tumor and surgery were analyzed retrospectively. Results: Sixty patients (87%) were female and nine were male. Among women, 83% had a history of contraceptive use;among men, only one had an androgen intake history. Overall mean age was 36.4 years (15 - 49), with men older than women (33.9 ± 8.14 years vs. 40.4 ± 6.27 years;P = 0.02). Forty one patients reported abdominal pain, associated or not to other symptoms;32% had an episode of hemorrhage;28 were asymptomatic with an incidental radiological finding. In total, 45 patients presented only one lesion and overall mean size was 8.1 cm (2 - 31);tumors were larger among men (mean size 12.9 ± 9.86 cm vs. 7.7 ± 4.58 cm;P = 0.009). Twenty one surgeries were laparoscopic. Hepatocellular carcinoma (HCC) was identified in three specimens and the incidence was higher among men (22.2% vs. 1.6%;P = 0.042) and in tumors larger than 20 cm (66.6% vs. 0.02%). There was no perioperative mortality. Two of the three patients with HCC died with extrahepatic recurrence;the remaining patient is well after 36 months. Overall mean follow-up time was 14.2 months (2 - 76). Conclusion: Male patients with HCA were older and had larger tumors when compared to females. Incidence of HCC was higher among men and in lesions larger than 20 cm. Male patients with HCA should be treated more aggressively than females.展开更多
The magnitude of river morphological changes are better analyzed through the use of quantitative approaches, wherein resolution accuracy and uncertainty assessment are treated as crucial key-factors. In this sense, th...The magnitude of river morphological changes are better analyzed through the use of quantitative approaches, wherein resolution accuracy and uncertainty assessment are treated as crucial key-factors. In this sense, the creation of precise DEMs （Digital Elevation Models） of rivers represents an affordable tool to analyze geomorphic variations and budgets, except for wetted areas, where reliable channel digitalization can normally be obtained only using expensive bathymetric surveys. The proposed work aims at improving channel surface models without having available bathymetric sensors, by deriving dry areas elevations from LiDAR data and water depth of wetted areas from aerial photos through a predictive depth-colour relationship. The methodology was applied to two different sub-reaches of the Piave River, a gravel-bed river which suffered severe flood events in 2010. Erosion and deposition patterns were identified through DEM differencing, showing a predominance of scour processes which can lead to channel instability situations. The bathymetric output was compared to other previously-derived models confirming the accuracy of the in-channel elevation estimates. Finally, a discussion on the role played by longitudinal protections during the studied flood events is proposed, focusing the attention on the incidence of two major bank erosions that removed significant volumes of stable areas.展开更多
Based on the most abundant metal of aluminum （Al）, the concept of rechargeable aluminum-ion batteries （AIBs） has been proposed for a few decades, offering the possibilities to replace the benchmark lithium-ion bat...Based on the most abundant metal of aluminum （Al）, the concept of rechargeable aluminum-ion batteries （AIBs） has been proposed for a few decades, offering the possibilities to replace the benchmark lithium-ion batteries （LIBs） in portable electronics and large-scale grid for energy storage. However, a number of problems such as the lack of proper cathode materials, low working voltage, insufficient capacity and poor cycling stability have hindered the AIBs from practical applications . In a paper recently published in Nature, Dai＇s group had made a break-through on a new AIB system in conceptual cell design and electrochemical performance.展开更多
The estimation of underwater features of channel bed surfaces without the use of bathymetric sensors results in very high levels of uncertainty. A revised approach enabling an automatic extraction of the wet areas to ...The estimation of underwater features of channel bed surfaces without the use of bathymetric sensors results in very high levels of uncertainty. A revised approach enabling an automatic extraction of the wet areas to create more accurate and detailed Digital Terrain Models (DTMs) is here presented. LiDAR-derived elevations of dry surfaces, water depths of wetted areas derived from aerial photos and a predictive depth-colour relationship were adopted. This methodology was applied at two different reaches of a northeastern Italian gravel-bed river (Tagliamento) before and after two flood events occurred in November and December 2010. In-channel dGPS survey points were performed taking different depth levels and different colour scales of the river bed. More than 10,473 control points were acquired, 1107 in 2010 and 9366 in 2011 respectively. A regression model that calculates channel depths using the correct intensity of three colour bands (RGB) was implemented. LiDAR and water depth points were merged and interpolated into DTMs which features an average error, for the wet areas, of ±14 cm. The different number of calibration points obtained for 2010 and 2011 showed that the bathymetric error is also sensitive to the number of acquired calibration points. The morphological evolution calculated through a difference of DTMs shows a prevalence of deposition and erosion areas into the wet areas.展开更多
Regenerating gene (Reg or REG) family, within the superfamily of C-type lectin, is mainly involved in the liver,pancreatic, gastric and intestinal cell proliferation or differentiation. Considerable attention has focu...Regenerating gene (Reg or REG) family, within the superfamily of C-type lectin, is mainly involved in the liver,pancreatic, gastric and intestinal cell proliferation or differentiation. Considerable attention has focused on Reg family and its structurally related molecules. Over the last 15 years, 17 members of the Reg family have been cloned and sequenced. They have been considered as members of a conserved protein family sharing structural and some functional properties being involved in injury, inflammation,diabetes and carcinogenesis. We previously identified Reg Ⅳ as a strong candidate for a gene that was highly expressed in colorectal adenoma when compared to normal mucosa based on suppression subtractive hybridization (SSH),reverse Northern blot, semi-quantitative reverse transcriptase PCR (RT-PCR)and Northern blot. In situ hybridization results further support that overexpression of Reg Ⅳ may be an early event in colorectal carcinogenesis. We suggest that detection of Reg Ⅳ overexpression might be useful in the early diagnosis of carcinomatous transformation of adenoma.This review summarizes the roles of Reg family in diseases in the literature as well as our recent results of Reg Ⅳ in colorectal cancer. The biological properties of Reg family and its possible roles in human diseases are discussed. We particularly focus on the roles of Reg family as sensitive reactants of tissue injury, prognostic indicators of tumor survival and early biomarkers of carcinogenesis. In addition to our current understanding of Reg gene functions, we postulate that there might be relationships between Reg family and microsatellite instability, apoptosis and cancer with a poor prognosis. Investigation of the correlation between tumor Reg expression and survival rate, and analysis of the Reg gene status in human maliganancies, are required to elucidate the biologic consequences of Reg gene expression, the implications for Reg gene regulation of cell growth, tumorigenesis, and the progression of cancer. It needs to be f展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.40772134)Open Research Program of the State Key Laboratory of Geological Processes and Mineral Resources,China University of Geosciences(Grant No.200643)
文摘The dating of the uplift onset of the Huangshan pluton in the southern part of Anhui Province associated with the collision between paleo-Pacific Block and the Eurasian Block is a fundamental issue to better understand the uplift mechanisms and the regional tectonic evolution. Х^2 values of seven zircon fission track （ZFF） samples collected from the south part of the Huangshan pluton were all 〈5%. Based on the grain ages of four typical ZFr samples, three thermal overprints ranging in 113-123, 72-95 and 49-66 Ma were distinguished respectively using the binomial peak-fitting method in accordance with the tectonic thermal events after south China shifted into circum-Pacific tectonic region. Apatite fission track （AFT） study of the Huangshan pluton shows ages lie between 15±3 and 56±6 Ma with all Х^2 values 〉5%, and all are significantly younger than their host rock formation ages, indicating that the samples have experienced post-formation thermal history. Based on the AFT resuits and topography characteristics in the Huangshan pluton, three zones with different denudation intensities caused by the differential uplift of the fault blocks were recognized in this paper, indicating that denudation was driven by the increase in elevation significantly. Modeling of the AFT data shows that the earlier cooling phase took place in the early Cenozoic and involved 3.3, 3.8 and 6.0℃/Ma of cooling rates equivalent to the exhumation rates of 90, 104 and 167 m/Ma in different fault blocks. The last phase of cooling took place from -10 Ma with average cooling rate of -5.6℃/Ma, equivalent to average denudation rates of -156 m/Ma. The fission track results imply that the regional compression field plays an important role for the differential exhumation between individual fault blocks of the Huangshan pluton.
基金This study was supported by the National Natural Science Foundation of China(Nos.81741007 and 81870363)the Science&Technology Departments of Sichuan Province(No.2020JDTD0025)the Grant from Chengdu University of Traditional Chinese Medicine(Nos.008066,030038199,BJRC2018001/030041023,030041224,ZKYY2004/030055180 and 242030016).
文摘Pathological vascular remodeling is a hallmark of various vascular diseases.Previous research has established the significance of andrographolide in maintaining gastric vascular homeostasis and its pivotal role in modulating endothelial barrier dysfunction,which leads to pathological vascular remodeling.Potassium dehydroandrographolide succinate(PDA),a derivative of andrographolide,has been clinically utilized in the treatment of inflammatory diseases precipitated by viral infections.This study investigates the potential of PDA in regulating pathological vascular remodeling.The effect of PDA on vascular remodeling was assessed through the complete ligation of the carotid artery in C57BL/6 mice.Experimental approaches,including rat aortic primary smooth muscle cell culture,flow cytometry,bromodeoxyuridine(BrdU)incorporation assay,Boyden chamber cell migration assay,spheroid sprouting assay,and Matrigel-based tube formation assay,were employed to evaluate the influence of PDA on the proliferation and motility of smooth muscle cells(SMCs).Molecular docking simulations and co-immunoprecipitation assays were conducted to examine protein interactions.The results revealed that PDA exacerbates vascular injury-induced pathological remodeling,as evidenced by enhanced neointima formation.PDA treatment significantly increased the proliferation and migration of SMCs.Further mechanistic studies disclosed that PDA upregulated myeloid differentiation factor 88(MyD88)expression in SMCs and interacted with T-cadherin(CDH13).This interaction augmented proliferation,migration,and extracellular matrix deposition,culminating in pathological vascular remodeling.Our findings underscore the critical role of PDA in the regulation of pathological vascular remodeling,mediated through the MyD88/CDH13 signaling pathway.
文摘Li-rich cathode materials have been considered as promising candidates for high-energy lithium ion batteries （LIBs）. In this study, we report a new series of Li-rich materials （Li[Li1/B-2x/BMn2/3-x/3Nix]O2 （0.09 ≤x≤ 0.2）） doped with small amounts of Ni as cathode materials in LIBs, which exhibited unusual phenomenon of capacity increase up to tens of cycles due to the continuous activation of the Li2MnO3 phase. Both experimental and computational results indicate that unlike commonly studied Ni-doped Li-rich cathode materials, smaller amounts of Ni doping can promote the stepwise Li2MnO3 activation to obtain increased specific capacity and better cycling capability. In contrast, excessive Ni will over-activate the Li2MnO3 and result in a large capacity loss in the first cycle. The Lil.25Mn0.625Ni0.12sO2 material with an optimized content of Ni delivered a superior high capacity of -280 mAh.g-1 and good cycling stability at room temperature.
基金financially supported by National Natural Science Foundation of China(U21A20198 and 32100658)the National Key R&D Program of China(2018YFA0800502)。
文摘Mutations in calcium-dependent papain-like protease CALPAIN3(CAPN3)cause Limb-Girdle Muscular Dystrophy Recessive Type 1(LGMDR1),the most common limb-girdle muscular dystrophy in humans.In addition to progressive muscle weakness,persistent inflammatory infiltration is also a feature of LGMDR1.Despite the underlying mechanism remaining poorly understood,we consider that it may relate to the newly defined role of CAPN3/Capn3b in the nucleolus.Here,we report that the loss of function of zebrafish capn3b,the counterpart of human CAPN3,induces an autoimmune response akin to that in LGMDR1 patients.capn3b mutant larvae are more susceptible to Listeria monocytogenes injection,characterized by recruiting more macrophages.Under germ-free conditions,transcriptome analysis of the capn3b mutant muscle reveals a significant upregulation of the chemokine-production-related genes.Coincidently,more neutrophils are recruited to the injury site imposed by either muscle stabbing or tail fin amputation.Nucleolar proteomic analysis and enzymatic assays reveal NKAP,an activating factor of the NF-k B pathway,to be a target of CAPN3.We conclude that the accumulation of Nkap and other factors in the capn3b mutant may be involved in the over-activation of innate immunity.Our studies indicate that the zebrafish capn3b mutant is a powerful model for studying the immunity-related progression of human LGMDR1.
基金financially supported by the National Natural Science Foundation of China(No.51641206)Shandong Natural Science Foundation Project(No.ZR2015EM013)+1 种基金Special Funds for Independent Innovation and Transformation of Achievements in Shandong Province(No.2014CGZH0911)National Key R&D Program of China(No.2016YFB0100508)
文摘Li4Ti5012 (LTO) with rich R-TiO2 (17.06, 23.69, and 34.42 wt%), namely, R-TiO2@Li4Ti5O12 composites, were synthesized using the hydrothermal method and tetrabutyl titanate (TBT) as the precursor. Rietveld refinement of X-ray diffraction (XRD) results show that the proportion of Li occupying 16d sites is extraordinary low and the lattice constants of LTO and R-TiO2 change with the ritanium dioxide content. EIS measurements showed that with in creasing R-TiO2 content, both its charge transfer impedance (Rct) and lithium ion diffusion coefficient (DLi) decreased. The changes of Rct and DLi caused by the increase of titanium dioxide content have synergic-antagonistic effects on the rate and cycle properties of Li4Ti5012. The rate performance is positively related to DLi, while the cycle property is negatively correlated with Rct, indicati ng that the rate performs nee is mainly related to DLi, while Rct more significantly affects the cycle performance. LTO-RT-17.06% exhibited excellent rate properties, especially under a high current density (5.0 C, 132.5 mAh/g) and LTO-RT-34.42% showed superior long-term cycle performance (0.012% capacity loss per cycle) compared to that of LTO-RT-17.06% and LTO-RT-23.69%.
基金supported in part by National Key R&D Program of China(Grant No.2021YFB2802300)National Natural Science Foundation of China(Grant No.62075093 and 62211530039)+3 种基金Guangdong Innovative and Entrepreneurial Research Team Program(Grant No.2017ZT07C071)Shenzhen Science and Technology Innovation Commission(Grant No.JCYJ20220818100413030)Shenzhen Development and Reform Commission(Grant No.XMHT20220114005)funded by the Key Lab of Modern Optical Technologies of Education Ministry of China,Soochow University(Grant No.KJS2132).
文摘Microlenses or arrays are key elements in many applications.However,their construction methods involve multiple fabrication processes,thereby increasing the complexity and cost of fabrication.In this study,we demonstrate an optically anisotropic,electrically tunable liquid crystal(LC)microlens array using a simple,one-step fabrication method.The microlens array is formed via photopolymerization-induced phase separation inside a polymer/LC composite.It possesses both polarization-dependent and electrically tunable focusing and imaging properties.Without applying voltage,the microlens array has a natural focal length of 8 mm,which is a result of its inherent gradient refractive index profile.Upon applying voltage above the threshold,the LC molecules reorient along the electric field direction and the focal length of the microlens array gradually increases.Based on its superior properties,the microlens array is further used for integral imaging applications,demonstrating electrically tunable central depth plane.Such LC microlens arrays could find numerous potential applications owing to their advantageous features of being flat,ultra-thin,and tunable,including 3D displays,optical interconnects,and more.
基金supported by the China National Postdoctoral Program for Innovative Talents(BX20220384)the National Natural Science Foundation of China(52175301)。
文摘Solid-state lithium batteries(SSLBs)solve safety issues and are potentially energy-dense alternatives to next-generation energy storage systems.Battery green recycling routes are responsible for the widespread use of SSLBs due to minimizing environmental contamination,reducing production costs,and providing a sustainable solution for resources,e.g.,saving rare earth elements(La,Ta,etc.).Herein,a solid-state recycling strategy is proposed to achieve green recycling of the crucial component solidstate electrolytes(SSEs)in spent SSLBs.The short-circuited garnet Li_(6.5)La_(3)Zr_(1.5)Ta_(0.5)O_(12)(LLZTO)is broken into fine particles and mixed with fresh particles to improve sintering activity and achieve high packing density.The continuous Li absorption process promotes sufficient grain fusion and guarantees the transformation from tetragonal phase to pure cubic phase for high-performance recycled LLZTO.The Li-ion conductivity reaches 5.80×10^(-4)S cm-1with a relative density of 95.9%.Symmetric Li cell with asrecycled LLZTO shows long-term cycling stability for 700 h at 0.3 mA cm^(-2)without any voltage hysteresis.Full cell exhibits an excellent cycling performance with a discharge capacity of 141.5 mA h g^(-1)and a capacity retention of 92.1%after 400 cycles(0.2C).This work develops an environmentally friendly and economically controllable strategy to recycle SSE from spent SSLBs,guiding future directions of SSLBs large-scale industrial application and green recycling study.
基金This study was supported by the National Key R&D Program of China and the Natural Science Foundation of China in the order of 2018YFA0800502,31830113 and 2017YFA0504501.
文摘Recovery of liver mass to a healthy liver donor by compensatory regeneration after partial hepatectomy(PH)is a prerequisite for liver transplantation.Synchronized cell cycle reentry of the existing hepatocytes after PH is seemingly a hallmark of liver compensatory regeneration.Although the molecular control of the PH-triggered cell cycle reentry has been extensively studied,little is known about how the synchronization is achieved after PH.The nucleolus-localized protein cleavage complex formed by the nucleolar protein Digestive-organ expansion factor(Def)and cysteine proteinase Calpain 3(Capn3)has been implicated to control wounding healing during liver regeneration through selectively cleaving the tumor suppressor p53 in the nucleolus.However,whether the Def-Capn3 complex participates in regulating the synchronization of cell cycle reentry after PH is unknown.In this report,we generated a zebrafish capn3b null mutant(capn3b^(Δ19Δ14)).The homozygous mutant was viable and fertile,but suffered from a delayed liver regeneration after PH.Delayed liver regeneration in capn3b^(Δ19Δ14)was due to disruption of synchronized cell proliferation after PH.Mass spectrometry(MS)analysis of nuclear proteins revealed that a number of negative regulators of cell cycle are accumulated in the capn3b^(Δ19Δ14)liver after PH.Moreover,we demonstrated that Check-point kinase 1(Chk1)and Wee1,two key negative regulators of G2 to M transition,are substrates of Capn3.We also demonstrated that Chk1 and Wee1 were abnormally accumulated in the nucleoli of amputated capn3bΔ19Δ14 liver.In conclusion,our findings suggest that the nucleolar-localized Def-Capn3 complex acts as a novel regulatory pathway for the synchronization of cell cycle reentry,at least partially,through inactivating Chk1 and Wee1 during liver regeneration after PH.
基金the National Key R&D Program of China and the Natural Science Foundation of China in the order of 2018YFA0800502,2017YFA0504501,31330050.
文摘The nucleolus,as the‘nucleus of the nucleus’,is a prominent subcellular organelle in a eukaryocyte.The nucleolus serves as the centre for ribosome biogenesis,as well as an important site for cell-cycle regulation,cellular senescence,and stress response.The protein composition of the nucleolus changes dynamically through protein turnover to meet the needs of cellular activities or stress responses.Recent studies have identified a nucleolus-localized protein degradation pathway in zebrafish and humans,namely the Def-CAPN3 pathway,which is essential to ribosome production and cell-cycle progression,by controlling the turnover of multiple substrates(e.g.,ribosomal small-subunit[SSU]processome component Mpp10,transcription factor p53,check-point proteins Chk1 and Wee1).This pathway relies on the Ca2þ-dependent cysteine proteinase CAPN3 and is independent of the ubiquitin-mediated proteasome pathway.CAPN3 is recruited by nucleolar protein Def from cytoplasm to nucleolus,where it proteolyzes its substrates which harbor a CAPN3 recognition-motif.Def depletion leads to the exclusion of CAPN3 and accumulation of p53,Wee1,Chk1,and Mpp10 in the nucleolus that result in cell-cycle arrest and rRNA processing abnormality.Here,we summarize the discovery of the Def-CAPN3 pathway and propose its biological role in cell-cycle control and ribosome biogenesis.
基金Supported by the Key Sci-Tech Research Project of Shaanxi Province(2006K12-G3)
文摘Objective： To establish an experimental model of urethral stricture in rabbits. Methods： A total of 21 adult male New Zealand rabbits were included into group. After intravenous anesthesia, urethroscopy was performed with a pediatric resectoscope （F13）. Fifteen animals were randomly selected as the study group. A lcm-long circumferential electrocoagulation of the bulbar urethra was performed to these animals until ulceration of the mucosa. The remaining 6 animals underwent video urethroscopy without electrocoagulation, serving as controls. On the 30th day, retrograde urethrogram and urethroscopy were performed to evaluate urethral stricture formation, histological examinations （HE and Sirius Red staining） were done to assess urethral pathological change. Results： Two rabbits in study group died and no death occurred in controls. Based on urethrogram and urethroscopy, no rabbits in control group developed urethral stricture, while significant stricture formation was observed in every case of the study group. Histological examination showed a normal urethra in control cases, while at stricture site of the study group extensive fibrosis of muscle and submucous tis High collagen expression in fibrosis tissue was assayed sue by was observed with a large number of fibroblasts infiltration Sirius Red staining. Conclusion： A lcm-long endoscopic electrocoagulation can successfully induce urethral stricture formation in rabbit models. This method offers an ideal animal model for the fundamental and clinical study of urethral stricture
文摘This paper presents partially asynchronous parallel simulation of continuous-system (PAPSoCS) and some approaches to the issues of its implementation on a multicomputer system. To guarantee the simulation results correct and speedup the simulation, the scheme for efficient PAPSoCS is proposed and the virtual topology star is constructed to match the path of message passing for solving algorithm-architecture adequation problem. Under the circumstances that messages frequently passed inter-processor are much shorter, typically within several 4 bytes, asynchronous communication mode is employed to reduce the communication ratio. Experiment results show that asynchronous parallel simulation has much higher efficiency than its synchronous counterpart.
文摘In this paper, a homogenous parallel simulation system is presented in detail for continuous--system simulation. The system is collstructed by a host computer and I I transputers connected into a topologyof 'Super--Node' which is very suitable for simulation of stiff systems. An automatic software interface runin the host is developed to partition simulation model, either equations or block diagrams, into several equitable segments and then pack them into parallel simulation program to be executed in the parallel system.This interface frees simulation users from parallel programming to focus on their simulation experiments.
文摘High performance of parallel computing on a message-passing multicomputer System relies on the balance of the workloads located on the processing elements of the System and the minimum communication ovcrheads among them. Mapping is the technology to partition the problem domain wellbalanced into multiple distinct execution tasks based on some measures. In mapping, a good objective function is the criterion to guarantce the distinct execution tasks equitable. In this paper, we evaluate five categories of those existed objective functions with three different problem subjects using experiments and find an objective function is much suitable for all kinds of problems.
文摘In today’s era,multidisciplinary integration is the development trend of education,which breaks the field of a single discipline and lays the foundation for the development of education.Objectively speaking,with the integration of disciplines,a new interdisciplinary is formed.This new discipline is comprehensive and systematic;in addition,it integrates knowledge,technology,and methods.It is the result of the collision of different disciplines.As an applied course,Management Science and Engineering integrates multidisciplinary knowledge,such as science and technology,information science,and mathematics.Its main purpose is to solve various problems in social and economic development.It has a long-term impact on the development of contemporary multidisciplinary education.In the new era,in order to meet the requirements of social and economic development,the teaching of Management Science and Engineering should be based on the perspective of multidisciplinary integration,combined with the current characteristics and training mode of the discipline,explore new talent training paths,and then drive the development of the whole industry.
文摘Background: Hepatocellular adenoma is a rare liver tumor that may require surgical treatment in cases of hemorrhage or suspicion of malignant lesions. Aim: To analyze data from patients who underwent hepatectomy for hepatocellular adenoma (HCA) in Rio de Janeiro, Brazil. Methods: From January 2005 to March 2019, sixty-nine patients with HCA underwent hepatectomy at centers in Rio de Janeiro. They were included in the analysis patients undergoing hepatectomy with pathological diagnosis of hepatocellular adenoma and excluded patients with hepatectomy with anatomopathological diagnosis other than hepatocellular adenoma, mainly nodular focal hyperplasia. Data related to patients, tumor and surgery were analyzed retrospectively. Results: Sixty patients (87%) were female and nine were male. Among women, 83% had a history of contraceptive use;among men, only one had an androgen intake history. Overall mean age was 36.4 years (15 - 49), with men older than women (33.9 ± 8.14 years vs. 40.4 ± 6.27 years;P = 0.02). Forty one patients reported abdominal pain, associated or not to other symptoms;32% had an episode of hemorrhage;28 were asymptomatic with an incidental radiological finding. In total, 45 patients presented only one lesion and overall mean size was 8.1 cm (2 - 31);tumors were larger among men (mean size 12.9 ± 9.86 cm vs. 7.7 ± 4.58 cm;P = 0.009). Twenty one surgeries were laparoscopic. Hepatocellular carcinoma (HCC) was identified in three specimens and the incidence was higher among men (22.2% vs. 1.6%;P = 0.042) and in tumors larger than 20 cm (66.6% vs. 0.02%). There was no perioperative mortality. Two of the three patients with HCC died with extrahepatic recurrence;the remaining patient is well after 36 months. Overall mean follow-up time was 14.2 months (2 - 76). Conclusion: Male patients with HCA were older and had larger tumors when compared to females. Incidence of HCC was higher among men and in lesions larger than 20 cm. Male patients with HCA should be treated more aggressively than females.
文摘The magnitude of river morphological changes are better analyzed through the use of quantitative approaches, wherein resolution accuracy and uncertainty assessment are treated as crucial key-factors. In this sense, the creation of precise DEMs （Digital Elevation Models） of rivers represents an affordable tool to analyze geomorphic variations and budgets, except for wetted areas, where reliable channel digitalization can normally be obtained only using expensive bathymetric surveys. The proposed work aims at improving channel surface models without having available bathymetric sensors, by deriving dry areas elevations from LiDAR data and water depth of wetted areas from aerial photos through a predictive depth-colour relationship. The methodology was applied to two different sub-reaches of the Piave River, a gravel-bed river which suffered severe flood events in 2010. Erosion and deposition patterns were identified through DEM differencing, showing a predominance of scour processes which can lead to channel instability situations. The bathymetric output was compared to other previously-derived models confirming the accuracy of the in-channel elevation estimates. Finally, a discussion on the role played by longitudinal protections during the studied flood events is proposed, focusing the attention on the incidence of two major bank erosions that removed significant volumes of stable areas.
文摘Based on the most abundant metal of aluminum （Al）, the concept of rechargeable aluminum-ion batteries （AIBs） has been proposed for a few decades, offering the possibilities to replace the benchmark lithium-ion batteries （LIBs） in portable electronics and large-scale grid for energy storage. However, a number of problems such as the lack of proper cathode materials, low working voltage, insufficient capacity and poor cycling stability have hindered the AIBs from practical applications . In a paper recently published in Nature, Dai＇s group had made a break-through on a new AIB system in conceptual cell design and electrochemical performance.
文摘The estimation of underwater features of channel bed surfaces without the use of bathymetric sensors results in very high levels of uncertainty. A revised approach enabling an automatic extraction of the wet areas to create more accurate and detailed Digital Terrain Models (DTMs) is here presented. LiDAR-derived elevations of dry surfaces, water depths of wetted areas derived from aerial photos and a predictive depth-colour relationship were adopted. This methodology was applied at two different reaches of a northeastern Italian gravel-bed river (Tagliamento) before and after two flood events occurred in November and December 2010. In-channel dGPS survey points were performed taking different depth levels and different colour scales of the river bed. More than 10,473 control points were acquired, 1107 in 2010 and 9366 in 2011 respectively. A regression model that calculates channel depths using the correct intensity of three colour bands (RGB) was implemented. LiDAR and water depth points were merged and interpolated into DTMs which features an average error, for the wet areas, of ±14 cm. The different number of calibration points obtained for 2010 and 2011 showed that the bathymetric error is also sensitive to the number of acquired calibration points. The morphological evolution calculated through a difference of DTMs shows a prevalence of deposition and erosion areas into the wet areas.
基金National Natural Science Foundation of China,No.30200333 and No.30371605
文摘Regenerating gene (Reg or REG) family, within the superfamily of C-type lectin, is mainly involved in the liver,pancreatic, gastric and intestinal cell proliferation or differentiation. Considerable attention has focused on Reg family and its structurally related molecules. Over the last 15 years, 17 members of the Reg family have been cloned and sequenced. They have been considered as members of a conserved protein family sharing structural and some functional properties being involved in injury, inflammation,diabetes and carcinogenesis. We previously identified Reg Ⅳ as a strong candidate for a gene that was highly expressed in colorectal adenoma when compared to normal mucosa based on suppression subtractive hybridization (SSH),reverse Northern blot, semi-quantitative reverse transcriptase PCR (RT-PCR)and Northern blot. In situ hybridization results further support that overexpression of Reg Ⅳ may be an early event in colorectal carcinogenesis. We suggest that detection of Reg Ⅳ overexpression might be useful in the early diagnosis of carcinomatous transformation of adenoma.This review summarizes the roles of Reg family in diseases in the literature as well as our recent results of Reg Ⅳ in colorectal cancer. The biological properties of Reg family and its possible roles in human diseases are discussed. We particularly focus on the roles of Reg family as sensitive reactants of tissue injury, prognostic indicators of tumor survival and early biomarkers of carcinogenesis. In addition to our current understanding of Reg gene functions, we postulate that there might be relationships between Reg family and microsatellite instability, apoptosis and cancer with a poor prognosis. Investigation of the correlation between tumor Reg expression and survival rate, and analysis of the Reg gene status in human maliganancies, are required to elucidate the biologic consequences of Reg gene expression, the implications for Reg gene regulation of cell growth, tumorigenesis, and the progression of cancer. It needs to be f
