Exposure to fine ambient particulate matter(PM_(2.5)) is known to be associated with cardiovascular disease. To uncover the molecular mechanisms involved in cardiovascular toxicity of PM_(2.5), we investigated alterat...Exposure to fine ambient particulate matter(PM_(2.5)) is known to be associated with cardiovascular disease. To uncover the molecular mechanisms involved in cardiovascular toxicity of PM_(2.5), we investigated alterations in the protein profile of human umbilical vein endothelial cells(HUVECs) treated with PM_(2.5) using two-dimensional electrophoresis in conjunction with mass spectrometry(MS). A total of 31 protein spots were selected as differentially expressed proteins and identified by matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF) MS. The results demonstrated that DNA damage and cell apoptosis are important factors contributing to PM_(2.5)-mediated toxicity in HUVECs. It is further proposed that PM_(2.5) can inhibit superoxide dismutase(SOD) activity and increase reactive oxygen species(ROS) and malonaldehyde(MDA) production in a concentration-dependent manner. Induction of apoptosis and DNA damage through oxidative stress pathways may be one of the key toxicological events occurring in HUVECs under PM_(2.5) stress. These results indicated that the toxic mechanisms of PM_(2.5) on cardiovascular disease are related to endothelial dysfunction.展开更多
目的通过对苏皖地区健康体检者的血清总IgE(tIgE)和特异性IgE(sIgE)水平进行测定,分析探讨其临床价值及意义。方法本研究采用酶联免疫捕获法和荧光免疫法,分别对苏皖地区接受健康体检的379例志愿者(男207例,女172例,17~64岁,中位数27岁...目的通过对苏皖地区健康体检者的血清总IgE(tIgE)和特异性IgE(sIgE)水平进行测定,分析探讨其临床价值及意义。方法本研究采用酶联免疫捕获法和荧光免疫法,分别对苏皖地区接受健康体检的379例志愿者(男207例,女172例,17~64岁,中位数27岁)行血清tIgE和sIgE测定。血清tIgE水平≤100 k U/L为阴性,>100 k U/L为阳性;sIgE采用吸入性变应原过筛试验(Phadiatop),<0.35 k U/L(0级)为阴性,≥0.35 k U/L(1级以上)为阳性。使用SPSS 18.0统计软件分析数据,P<0.05表示差异有统计学意义,组间比较进行矫正(α=0.05/6=0.008 3)。结果 379例健康志愿者中,血清tIgE水平介于1.48~1 903.22(中位数53.70)k U/L,男性显著高于女性(P<0.001),其中tIgE阳性和阴性者分别为129例(34.04%)和250例(65.96%);血清sIgE水平范围为0.01~45.20(中位数0.07)k U/L,男女之间无明显差异(P=0.207),其中sIgE阳性和阴性者分别为88例(23.22%)和291例(76.78%)。进一步分析,四个年龄组中血清tIgE水平在17~30岁年龄组最高(中位数62.18 k U/L,P=0.006),且男性tIgE水平在该年龄组最高(中位数80.60 k U/L,P=0.009)。血清sIgE水平在四个年龄组中无明显差异(P=0.318)。血清sIgE阳性人群中有67例(76.14%)tIgE水平>100 k U/L,而sIgE阴性人群中有62例(21.31%)tIgE水平>100 k U/L。血清tIgE阴性人群中sIgE阴性者占绝对多数(91.60%,P<0.001)。Spearman秩相关分析显示,血清tIgE与sIgE水平的相关系数为0.650,呈正相关(P<0.001)。结论苏皖地区健康成人的血清tIgE与sIgE水平具有较强的的相关性。低水平的血清tIgE(100 k U/L以下)有助于排除变态反应(变应原致敏),可作为一个临床参考指标。展开更多
基金Supported by National Natural Science Foundation of China No.81000169,No.81100277,No.81370008,and No.81200284the Excellent Young Investigator Foundation of the Health Bureau of Zhejiang Province No.2010QNA011+2 种基金the Excellent Young Investigator Natural Science Foundation of Zhejiang Province No.R2110159the Project of Zhejiang Traditional Chinese Medicine Administration Bureau No.2010ZA065the Fundamental Research Funds for the Central Universities No.2013QNA702
文摘AIM: To explore mitochondrial dysfunction in nonalcoholic steatohepatitis (NASH) by analyzing the proteome of liver mitochondria from a NASH model.
基金Project supported by the Medical and Health Science and Technology Fund of Zhejiang Province(No.2016KYB224)the Scientific Research Fund of Zhejiang Chinese Medicine University(No.2015ZG17),China
文摘Exposure to fine ambient particulate matter(PM_(2.5)) is known to be associated with cardiovascular disease. To uncover the molecular mechanisms involved in cardiovascular toxicity of PM_(2.5), we investigated alterations in the protein profile of human umbilical vein endothelial cells(HUVECs) treated with PM_(2.5) using two-dimensional electrophoresis in conjunction with mass spectrometry(MS). A total of 31 protein spots were selected as differentially expressed proteins and identified by matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF) MS. The results demonstrated that DNA damage and cell apoptosis are important factors contributing to PM_(2.5)-mediated toxicity in HUVECs. It is further proposed that PM_(2.5) can inhibit superoxide dismutase(SOD) activity and increase reactive oxygen species(ROS) and malonaldehyde(MDA) production in a concentration-dependent manner. Induction of apoptosis and DNA damage through oxidative stress pathways may be one of the key toxicological events occurring in HUVECs under PM_(2.5) stress. These results indicated that the toxic mechanisms of PM_(2.5) on cardiovascular disease are related to endothelial dysfunction.
文摘目的通过对苏皖地区健康体检者的血清总IgE(tIgE)和特异性IgE(sIgE)水平进行测定,分析探讨其临床价值及意义。方法本研究采用酶联免疫捕获法和荧光免疫法,分别对苏皖地区接受健康体检的379例志愿者(男207例,女172例,17~64岁,中位数27岁)行血清tIgE和sIgE测定。血清tIgE水平≤100 k U/L为阴性,>100 k U/L为阳性;sIgE采用吸入性变应原过筛试验(Phadiatop),<0.35 k U/L(0级)为阴性,≥0.35 k U/L(1级以上)为阳性。使用SPSS 18.0统计软件分析数据,P<0.05表示差异有统计学意义,组间比较进行矫正(α=0.05/6=0.008 3)。结果 379例健康志愿者中,血清tIgE水平介于1.48~1 903.22(中位数53.70)k U/L,男性显著高于女性(P<0.001),其中tIgE阳性和阴性者分别为129例(34.04%)和250例(65.96%);血清sIgE水平范围为0.01~45.20(中位数0.07)k U/L,男女之间无明显差异(P=0.207),其中sIgE阳性和阴性者分别为88例(23.22%)和291例(76.78%)。进一步分析,四个年龄组中血清tIgE水平在17~30岁年龄组最高(中位数62.18 k U/L,P=0.006),且男性tIgE水平在该年龄组最高(中位数80.60 k U/L,P=0.009)。血清sIgE水平在四个年龄组中无明显差异(P=0.318)。血清sIgE阳性人群中有67例(76.14%)tIgE水平>100 k U/L,而sIgE阴性人群中有62例(21.31%)tIgE水平>100 k U/L。血清tIgE阴性人群中sIgE阴性者占绝对多数(91.60%,P<0.001)。Spearman秩相关分析显示,血清tIgE与sIgE水平的相关系数为0.650,呈正相关(P<0.001)。结论苏皖地区健康成人的血清tIgE与sIgE水平具有较强的的相关性。低水平的血清tIgE(100 k U/L以下)有助于排除变态反应(变应原致敏),可作为一个临床参考指标。
