AIM: To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer. METHODS: RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the ex...AIM: To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer. METHODS: RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and benign colon tissue from a single patient. RESULTS: The highest PDX-1 transcription levels were detected in the metastasis material. Lower levels of PDX-1 were found to be present in the primary tumor, while normal colon tissue failed to express detectable levels of PDX-1. Western blot data revealed a PDX-1 expression pattern identical to that of mRNA expression. Immunohistochemistry confirmed high metastasis PDX-1 expression, lower levels in the primary tumor, and the presence of only traces of PDX-1 in normal colon tissue. CONCLUSION: These data argue for further evaluation of PDX-1 as a biomarker for colorectal cancer.展开更多
Morphological variation based on eight measured and four derived traits was studied to establish whether there was significant variation between populations and to identify the influence of the altitude on morphologic...Morphological variation based on eight measured and four derived traits was studied to establish whether there was significant variation between populations and to identify the influence of the altitude on morphological differentiation among 44 natural stands of common oak（Quercus robur L.） in Bosnia and Herzegovina,ranging from 82 to 860 m.The results point to significant intra-and inter-population differences.Elevation-related variation is less pronounced and determined largely by microclimatic factors.The results could provide management strategies for species reintroduction in the study area.There are no differences in leaf morphology between the different branches of individual trees.However,there are important variations between the altitudinal groups and closely linked to environmental factors in all traits.An important recommendation is to use seeds from oak with attention to their altitudinal origin.展开更多
文摘AIM: To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer. METHODS: RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and benign colon tissue from a single patient. RESULTS: The highest PDX-1 transcription levels were detected in the metastasis material. Lower levels of PDX-1 were found to be present in the primary tumor, while normal colon tissue failed to express detectable levels of PDX-1. Western blot data revealed a PDX-1 expression pattern identical to that of mRNA expression. Immunohistochemistry confirmed high metastasis PDX-1 expression, lower levels in the primary tumor, and the presence of only traces of PDX-1 in normal colon tissue. CONCLUSION: These data argue for further evaluation of PDX-1 as a biomarker for colorectal cancer.
基金financially supported by the Cantonal Ministry of Education and Science,Sarajevo Canton,Agreement No.11-14-20059.1/07 of 27.12.2007
文摘Morphological variation based on eight measured and four derived traits was studied to establish whether there was significant variation between populations and to identify the influence of the altitude on morphological differentiation among 44 natural stands of common oak（Quercus robur L.） in Bosnia and Herzegovina,ranging from 82 to 860 m.The results point to significant intra-and inter-population differences.Elevation-related variation is less pronounced and determined largely by microclimatic factors.The results could provide management strategies for species reintroduction in the study area.There are no differences in leaf morphology between the different branches of individual trees.However,there are important variations between the altitudinal groups and closely linked to environmental factors in all traits.An important recommendation is to use seeds from oak with attention to their altitudinal origin.
