BACKGROUND We report a rare case of a large congenital hemangioma(CH)in the maxillofacial region in a female neonate that caused thrombocytopenia and heart failure.With close multidisciplinary collaboration,the congen...BACKGROUND We report a rare case of a large congenital hemangioma(CH)in the maxillofacial region in a female neonate that caused thrombocytopenia and heart failure.With close multidisciplinary collaboration,the congenital hemangioma was successfully resected with good results.CASE SUMMARY The patient was delivered at gestational age of 36 wk by cesarean section due to cephalopelvic disproportion and lack of onset of labor(birth weight:2630 g).A right-sided facial tumor was detected in the fetus during routine antenatal ultrasound examination of the mother at 32 wk of gestation.Physical examination revealed a 7 cm×7 cm×3 cm hard,dull purple-colored mass on the right maxillofacial region.The mass was tense and had prominent surface telangiectasias.Laboratory investigations revealed reduced hemoglobin and platelet count,and increased activated partial thromboplastin time,prothrombin time,and thrombin time.International normalized ratio,fibrin degradation products,and D-Dimer levels were significantly increased.Thromboelastography showed increased alpha angle,mean amplitude,and the clot formation speed.Thyroid-stimulating hormone level was significantly elevated.The patient was administered prednisone,propranolol,euthyrox,vitamin K1,milrinone,and digoxin.After operation,cefepime was administered for anti-infection and propranolol was prescribed at discharge.CONCLUSION We report a rare case of CH in the right maxillofacial region causing thrombocytopenia and heart failure.展开更多
Objective:To explore the inhihitive and apoptosis inductive effect of IL-24 genes on CD133^+laryngeal cancer cells in Hep-2 line.Methods:Human peripheral blood monocytes were isolated.The total RNA was extracted by us...Objective:To explore the inhihitive and apoptosis inductive effect of IL-24 genes on CD133^+laryngeal cancer cells in Hep-2 line.Methods:Human peripheral blood monocytes were isolated.The total RNA was extracted by using Trizol method and reverse transcripted into cDNA using RT-PCR method.Primers P1 and P2 was designed for the amplification of human IL-24 genes.After confirmation of agarose gel electrophoresis tests,TA was cloned into pMD19-T simple vector.Nhe Ⅰ and Xho Ⅰ double digesting human IL-24 and pIRES2-ZsGreen1 and eukaryotic expression vector were used to establish the pIRES2-ZsGreen1-hIL-24 vector,and detected by enzyme digestion and gene sequencing methods.Flow cytometry(FCM) was used to isolate CD133^+ cells from Hep-2 cells.CD133^+ cells were transfected with pIRES2-ZsGreen1-hIL-24 through liposome 2000.After detection,MTT and FCM were used to observe the effect of IL-24 gene on CD133^+ laryngeal cancer Hep-2 cells.Results:Lipotin mediated transfection of recombinant pIRES2-ZsGreen1-hIL-24 plasmid into CD133^+ Hep-2 could expressed IL-24 gene in cells stably.MTT results showed that IL-24 transfected group was significantly suppressed compared to empty vector group and control group(P<0.05);FCM results showed that the apoptosis rate of experimental group increased significantly compared to empty vector group and control group(P<0.05).Conclusions:IL-24 gene expressions can inhibit proliferation of CD133^+laryngeal cells in Hep-2 line and promote their apoptosis.展开更多
文摘BACKGROUND We report a rare case of a large congenital hemangioma(CH)in the maxillofacial region in a female neonate that caused thrombocytopenia and heart failure.With close multidisciplinary collaboration,the congenital hemangioma was successfully resected with good results.CASE SUMMARY The patient was delivered at gestational age of 36 wk by cesarean section due to cephalopelvic disproportion and lack of onset of labor(birth weight:2630 g).A right-sided facial tumor was detected in the fetus during routine antenatal ultrasound examination of the mother at 32 wk of gestation.Physical examination revealed a 7 cm×7 cm×3 cm hard,dull purple-colored mass on the right maxillofacial region.The mass was tense and had prominent surface telangiectasias.Laboratory investigations revealed reduced hemoglobin and platelet count,and increased activated partial thromboplastin time,prothrombin time,and thrombin time.International normalized ratio,fibrin degradation products,and D-Dimer levels were significantly increased.Thromboelastography showed increased alpha angle,mean amplitude,and the clot formation speed.Thyroid-stimulating hormone level was significantly elevated.The patient was administered prednisone,propranolol,euthyrox,vitamin K1,milrinone,and digoxin.After operation,cefepime was administered for anti-infection and propranolol was prescribed at discharge.CONCLUSION We report a rare case of CH in the right maxillofacial region causing thrombocytopenia and heart failure.
基金supported by Jilin Province Natural Science Foundation of China.No:20130101151JCChinese Ministry of Education Projects of Doctoral New Teachers.No:20120061120092
文摘Objective:To explore the inhihitive and apoptosis inductive effect of IL-24 genes on CD133^+laryngeal cancer cells in Hep-2 line.Methods:Human peripheral blood monocytes were isolated.The total RNA was extracted by using Trizol method and reverse transcripted into cDNA using RT-PCR method.Primers P1 and P2 was designed for the amplification of human IL-24 genes.After confirmation of agarose gel electrophoresis tests,TA was cloned into pMD19-T simple vector.Nhe Ⅰ and Xho Ⅰ double digesting human IL-24 and pIRES2-ZsGreen1 and eukaryotic expression vector were used to establish the pIRES2-ZsGreen1-hIL-24 vector,and detected by enzyme digestion and gene sequencing methods.Flow cytometry(FCM) was used to isolate CD133^+ cells from Hep-2 cells.CD133^+ cells were transfected with pIRES2-ZsGreen1-hIL-24 through liposome 2000.After detection,MTT and FCM were used to observe the effect of IL-24 gene on CD133^+ laryngeal cancer Hep-2 cells.Results:Lipotin mediated transfection of recombinant pIRES2-ZsGreen1-hIL-24 plasmid into CD133^+ Hep-2 could expressed IL-24 gene in cells stably.MTT results showed that IL-24 transfected group was significantly suppressed compared to empty vector group and control group(P<0.05);FCM results showed that the apoptosis rate of experimental group increased significantly compared to empty vector group and control group(P<0.05).Conclusions:IL-24 gene expressions can inhibit proliferation of CD133^+laryngeal cells in Hep-2 line and promote their apoptosis.
