Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was con...Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was conducted.Two triplicate groups of fish were fed identically either at 8:00(day)or at 20:00(night).After 9 weeks,three fish from each cage were sacrificed every 4 h for 24 h.The intestinal contents were analyzed using high-throughput sequencing.Three phyla(Fusobacteria,Proteobacteria,and Verrucomicrobia),and three genera(Cetobacterium,Akkermansia,and Clostridium)were dominant at every time point.Moreover,33.02%of detected amplicon sequence variants showed cyclical variations in fish fed at 20:00,and 3.47%in fish fed at 8:00.Among the top 20 genera,17 showed cyclical variations in the 20:00 group,and only two in the 8:00 group.Rhodobacter,Ralstonia,Mycobacterium,Lactococcus,Halomonas,Akkermansia,and Acinetobacter contributed more to the predicted functional pathways.Moreover,144 and 38 pathways exhibited daily cyclical fluctuations in fish fed at 20:00 and 8:00,respectively.Overall,certain intestinal microorganisms showed a circadian rhythm in P.fulvidraco,and this circadian rhythm was affected by shifted feeding times.展开更多
Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean.After whole genome duplications,about 75%of genes being represented by multiple copies in soybean.There are four TERMINA...Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean.After whole genome duplications,about 75%of genes being represented by multiple copies in soybean.There are four TERMINAL FLOWER 1(TFL1)genes in soybean,and the TFL1b(Dt1)has been characterized as the determinant of stem growth habit.The function of other TFL1 homologs in soybean is still unclear.Here,we generated knockout mutants by CRISPR/Cas9 genome editing technology and found that the tfl1c/tfl1d double mutants flowered significantly earlier than wild-type plants.We investigated that TFL1c and TFL1d could physically interact with the b ZIP transcription factor FDc1 and bind to the promoter of APETALA1a(AP1a).RNA-seq and q RT-PCR analyses indicated that TFL1c and TFL1d repressed the expressions of the four AP1 homologs and delayed the flowering time in soybean.The two genes play important roles in the regulation of flowering time in soybean and mainly act as the flowering inhibitors under long-day conditions.Our results identify novel components in the flowering-time regulation network of soybean and will be invaluable for molecular breeding of improved soybean yield.展开更多
A two-factor(23)orthogonal testwas conducted to investigate the effects of dietary myo-inositol(MI)on the osmoregulation and carbohydrate metabolism of euryhaline fish tilapia(Oreochromis niloticus)under sustained hyp...A two-factor(23)orthogonal testwas conducted to investigate the effects of dietary myo-inositol(MI)on the osmoregulation and carbohydrate metabolism of euryhaline fish tilapia(Oreochromis niloticus)under sustained hypertonic stress(20 practical salinity units[psu]).6 diets containing either normal carbohydrate(NC,30%)or high carbohydrate(HC,45%)levels,with 3 levels(0,400 and 1,200 mg/kg diet)of MI,respectively,were fed to 540 fish under 20 psu for 8 weeks.Dietary MI supplementation significantly improved growth performance and crude protein content of whole fish,and decreased the content of crude lipid of whole fish(P<0.05).Curled,disordered gill lamella and cracked gill filament cartilage were observed in the gill of fish fed diets without MI supplementation.The ion transport capacity in gill was significantly improved in the 1,200 mg/kg MI supplementation groups compared with the 0 mg/kg MI groups(P<0.05).Moreover,the contents of Na^(+),K^(+),Cl^(-)in serum weremarkedly reduced with the dietary MI supplementation(P<0.05).The fish fed 1,200 mg/kg MI supplementation had the highest MI content in the gills and the lowest MI content in the serum(P<0.05).Additionally,the fish fed with 1,200 mg/kg MI supplementation had the highest MI synthesis capacity in gills and brain(P<0.05).Dietary MI markedly promoted the ability of carbohydrate metabolism in liver(P<0.05).Moreover,fish in the 1,200 mg/kg MI groups had the highest antioxidant capacity(P<0.05).This study indicated that high dietary carbohydrate would intensify stress,and impair the ability of osmoregulation in tilapia under a long-term hypersaline exposure.The supplementation of MI at 1,200 mg/kg in the high carbohydrate diet could promote carbohydrate utilization and improve the osmoregulation capacity of tilapia under long-term hypertonic stress.展开更多
Circadian clock genes are crucial for generating and sustaining most rhythmic daily functions in the animal kingdom,which entrain the rhythms of biochemical,physiological,and behavioural processes.To better understand...Circadian clock genes are crucial for generating and sustaining most rhythmic daily functions in the animal kingdom,which entrain the rhythms of biochemical,physiological,and behavioural processes.To better understand the molecular oscillations of the circadian rhythms in darkbarbel catfish(Pelteobagrus vachellii),we isolated and characterized two circadian clock genes in P.vachellii,period 1(per1),and period 3(per3).The circadian clock gene per1 was found to encode a 1428-amino acid polypeptide,including PER-ARNT-SIM(PAS)dimerisation domains,a PAS-associated C-terminal motif(PAC),a short mutable domain(S/M),and a nuclear export signal(NES).The 4902-bp per3 cDNA includes an open reading frame encoding a 1292-amino acid residue polypeptide with a PER-ARNT-SIM(PAS)domain,cytoplasmic localisation domain(CLD),interaction site(TIS),and a nuclear localisation signal(NLS).The per1 and per3 gene was constitutively expressed in all examined tissues.Moreover,per1 expression within a light/dark cycles showed rhythmic expression in the diencephalon,brain,liver and intestine,with the acrophase at 15:15,12:52,7:51 and 12:55,respectively.Daily expression of per3 was rhythmic in the diencephalonbrain,liver and intestine,with the acrophase at 8:15,9:54,10:39,and 10:25 h,respectively.These findings expand our understanding of circadian mechanism at the molecular level in this species.展开更多
Nickel-based superalloys have become the critical materials of micro-parts depending on outstanding mechanical properties.The effects of the grain size and precipitates on the mechanical properties at the mesoscopic s...Nickel-based superalloys have become the critical materials of micro-parts depending on outstanding mechanical properties.The effects of the grain size and precipitates on the mechanical properties at the mesoscopic scale are difficult to be revealed using conventional macroscopic material constitutive models.In the present study,the microstructure evolution of the γ" phase and the tensile mechanical properties of a nickel-based superalloy at the mesoscopic scale were investigated systematically.Three variants of γ" phases precipitated corresponding to [00],[00] and [001] orientations of the matrix y phase.The quantitative statistics results showed that as the aging time increases,the particle size and volume fraction of the γ~" phase increase.As the grain size increases,the flow stress decreases due to the dwindling of grain boundary strengthening.Furthermore,the precipitation strengthening of γ" and γ" phases induces the increase of flow stress.An important conclusion is drawn that the size effect at the mesoscopic scale depends not only on the sample size and grain size but also on the particle size and volume fraction of the precipitates.The established constitutive model which considers grain boundary strengthening,precipitation strengthening and solid solution strengthening can accurately describe the flow stress characteristics of nickel-based superalloys at the mesoscopic scale.展开更多
Nickel-based superalloys have become the key materials of micro-parts depending on excellent mechanical properties at high temperatures.The plastic deformation behavior is difficult to predict due to the occurrence of...Nickel-based superalloys have become the key materials of micro-parts depending on excellent mechanical properties at high temperatures.The plastic deformation behavior is difficult to predict due to the occurrence of size effect on the mesoscopic scale.In this paper,the effect of specimen diameter to grain size ratio(D/d)on the flow stress and inhomogeneous plastic deformation behavior in compression of nickel-based superalloy cylindrical specimens was investigated on the mesoscopic scale.The results showed that when D/d is less than 9.7,the flow stress increases with the grain size.Aiming at this phenomenon,a flow stress size effect model considering compressive strain partitioning was established.The calculated flow stress values agree well with the experimental values,thus revealing the effect of D/d on the flow stress in compression of nickel-base superalloy on the mesoscopic scale.The inhomogeneous plastic deformation during compression deformation increases with the grain size.The end surface profiles evolve from a regular circular shape to an irregular shape with the grain size.The surface folding phenomenon occurs only in partially compressed specimen with a few grains across the diameter.Crystal plasticity finite-element(CPFE)simulation of compression deformation on the mesoscopic scale real-time displayed the evolution of microstructure.The study of this paper has important guiding significance for understanding the influence of D/d on the compression deformation behavior of nickel-based superalloy on the mesoscopic scale.展开更多
Effective tuning of nanoscale domain structures provides fundamental basis for controlling and engineering of various functionalities in ferroelectric materials.In this work,we demonstrate the precise patterning of na...Effective tuning of nanoscale domain structures provides fundamental basis for controlling and engineering of various functionalities in ferroelectric materials.In this work,we demonstrate the precise patterning of nanoscopic domain structures in as-grown epitaxial PbTiO_(3)(PTO)films by merely introducing an ultrathin pre-patterned doping layer(e.g.,Fe-doped PTO).The doping layer can effectively reverse the interfacial built-in bias,consequent to a reversed initial polarization reorientation in the as-grown film,which makes it possible to transfer the nano-patterns in the doping layer into the domain structure of ferroelectric films.For instance,we have successfully fabricated large area ordered array of nanoscale cylindrical domains(downward polarization)embedded in the matrix domain with opposite polarization(upward polarization)in PTO film.These nanoscale cylinder domains also allow deterministic and reversible erasure and creation induced by biased tip scanning.The results provide an effective pathway for on-demand patterning of large area nanoscale domains in the as-grown films,which may find applications in a wide range of nanoelectronic devices.展开更多
The sulphide ores of the Baimazhai deposit, although typically orthomagmatic, locally exhibit peculiar textural features and are intimately associated with hydrothermal minerals, such as biotite, amphibole and chlorit...The sulphide ores of the Baimazhai deposit, although typically orthomagmatic, locally exhibit peculiar textural features and are intimately associated with hydrothermal minerals, such as biotite, amphibole and chlorite. This association suggests that the magmatic sulphide ores were subjected to hydrothermal alteration and subsequent redistribution, resulting in the observed textural features. Geochemically, the Baimazhai sulphide ores are enriched in Cu, Pd and Au, which,according to previous studies, reflects the action of hydrothermal fluids. Interestingly, Ar-Ar dating yielded the plateau ages of about 160–170 Ma, which are at odds with the established Permian age of the Emeishan large igneous province. We interpreted these younger ages as due to thermal resetting during post-Permian tectonothermal events. We have proposed a model in which tectonic movements and hydrothermal fluids related to these events modified the pre-existing magmatic sulphides. Given the degree of overprint, we suggested two possible scenarios: 1) the sulphide disseminations that surround the massive magmatic ores are the result of deformation and hydrothermal alteration; and 2) there were both magmatic massive and disseminated sulphides, in which case the scale and relocation of remobilization would have been smaller, but still detectable.展开更多
基金Supported by the Sichuan Science and Technology Program(Nos.2021YFYZ0015,2023NSFSC0241)。
文摘Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was conducted.Two triplicate groups of fish were fed identically either at 8:00(day)or at 20:00(night).After 9 weeks,three fish from each cage were sacrificed every 4 h for 24 h.The intestinal contents were analyzed using high-throughput sequencing.Three phyla(Fusobacteria,Proteobacteria,and Verrucomicrobia),and three genera(Cetobacterium,Akkermansia,and Clostridium)were dominant at every time point.Moreover,33.02%of detected amplicon sequence variants showed cyclical variations in fish fed at 20:00,and 3.47%in fish fed at 8:00.Among the top 20 genera,17 showed cyclical variations in the 20:00 group,and only two in the 8:00 group.Rhodobacter,Ralstonia,Mycobacterium,Lactococcus,Halomonas,Akkermansia,and Acinetobacter contributed more to the predicted functional pathways.Moreover,144 and 38 pathways exhibited daily cyclical fluctuations in fish fed at 20:00 and 8:00,respectively.Overall,certain intestinal microorganisms showed a circadian rhythm in P.fulvidraco,and this circadian rhythm was affected by shifted feeding times.
基金supported by the National Natural Science Foundation of China(32022062,32001503)the Science and Technology Innovation Team of Soybean Modern Seed Industry in Hebei(21326313D)。
文摘Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean.After whole genome duplications,about 75%of genes being represented by multiple copies in soybean.There are four TERMINAL FLOWER 1(TFL1)genes in soybean,and the TFL1b(Dt1)has been characterized as the determinant of stem growth habit.The function of other TFL1 homologs in soybean is still unclear.Here,we generated knockout mutants by CRISPR/Cas9 genome editing technology and found that the tfl1c/tfl1d double mutants flowered significantly earlier than wild-type plants.We investigated that TFL1c and TFL1d could physically interact with the b ZIP transcription factor FDc1 and bind to the promoter of APETALA1a(AP1a).RNA-seq and q RT-PCR analyses indicated that TFL1c and TFL1d repressed the expressions of the four AP1 homologs and delayed the flowering time in soybean.The two genes play important roles in the regulation of flowering time in soybean and mainly act as the flowering inhibitors under long-day conditions.Our results identify novel components in the flowering-time regulation network of soybean and will be invaluable for molecular breeding of improved soybean yield.
基金grants from the National Natural Science Foundation of China(No.32172946)China Postdoctoral Science Foundation(2018 M630418)the Fundamental Research Funds for the Central Universities,ECNU and China Agriculture Research System-46(CARS-46).
文摘A two-factor(23)orthogonal testwas conducted to investigate the effects of dietary myo-inositol(MI)on the osmoregulation and carbohydrate metabolism of euryhaline fish tilapia(Oreochromis niloticus)under sustained hypertonic stress(20 practical salinity units[psu]).6 diets containing either normal carbohydrate(NC,30%)or high carbohydrate(HC,45%)levels,with 3 levels(0,400 and 1,200 mg/kg diet)of MI,respectively,were fed to 540 fish under 20 psu for 8 weeks.Dietary MI supplementation significantly improved growth performance and crude protein content of whole fish,and decreased the content of crude lipid of whole fish(P<0.05).Curled,disordered gill lamella and cracked gill filament cartilage were observed in the gill of fish fed diets without MI supplementation.The ion transport capacity in gill was significantly improved in the 1,200 mg/kg MI supplementation groups compared with the 0 mg/kg MI groups(P<0.05).Moreover,the contents of Na^(+),K^(+),Cl^(-)in serum weremarkedly reduced with the dietary MI supplementation(P<0.05).The fish fed 1,200 mg/kg MI supplementation had the highest MI content in the gills and the lowest MI content in the serum(P<0.05).Additionally,the fish fed with 1,200 mg/kg MI supplementation had the highest MI synthesis capacity in gills and brain(P<0.05).Dietary MI markedly promoted the ability of carbohydrate metabolism in liver(P<0.05).Moreover,fish in the 1,200 mg/kg MI groups had the highest antioxidant capacity(P<0.05).This study indicated that high dietary carbohydrate would intensify stress,and impair the ability of osmoregulation in tilapia under a long-term hypersaline exposure.The supplementation of MI at 1,200 mg/kg in the high carbohydrate diet could promote carbohydrate utilization and improve the osmoregulation capacity of tilapia under long-term hypertonic stress.
基金Supported by the National Natural Science Foundation of China(No.31402305)the Sichuan Science and Technology Program(Nos.2017JY0161,2018NZ0119)。
文摘Circadian clock genes are crucial for generating and sustaining most rhythmic daily functions in the animal kingdom,which entrain the rhythms of biochemical,physiological,and behavioural processes.To better understand the molecular oscillations of the circadian rhythms in darkbarbel catfish(Pelteobagrus vachellii),we isolated and characterized two circadian clock genes in P.vachellii,period 1(per1),and period 3(per3).The circadian clock gene per1 was found to encode a 1428-amino acid polypeptide,including PER-ARNT-SIM(PAS)dimerisation domains,a PAS-associated C-terminal motif(PAC),a short mutable domain(S/M),and a nuclear export signal(NES).The 4902-bp per3 cDNA includes an open reading frame encoding a 1292-amino acid residue polypeptide with a PER-ARNT-SIM(PAS)domain,cytoplasmic localisation domain(CLD),interaction site(TIS),and a nuclear localisation signal(NLS).The per1 and per3 gene was constitutively expressed in all examined tissues.Moreover,per1 expression within a light/dark cycles showed rhythmic expression in the diencephalon,brain,liver and intestine,with the acrophase at 15:15,12:52,7:51 and 12:55,respectively.Daily expression of per3 was rhythmic in the diencephalonbrain,liver and intestine,with the acrophase at 8:15,9:54,10:39,and 10:25 h,respectively.These findings expand our understanding of circadian mechanism at the molecular level in this species.
基金supported financially by the Training Program of the Major Research Plan of the National Natural Science Foundation of China(No.91860129)the National Natural Science Foundation of China(No.51575129)+1 种基金the Key Research and Development Program of Shandong Province(No.2016GGX102026)the Fundamental Research Funds for the Central Universities(No.HIT.NSRIF.2017064)。
文摘Nickel-based superalloys have become the critical materials of micro-parts depending on outstanding mechanical properties.The effects of the grain size and precipitates on the mechanical properties at the mesoscopic scale are difficult to be revealed using conventional macroscopic material constitutive models.In the present study,the microstructure evolution of the γ" phase and the tensile mechanical properties of a nickel-based superalloy at the mesoscopic scale were investigated systematically.Three variants of γ" phases precipitated corresponding to [00],[00] and [001] orientations of the matrix y phase.The quantitative statistics results showed that as the aging time increases,the particle size and volume fraction of the γ~" phase increase.As the grain size increases,the flow stress decreases due to the dwindling of grain boundary strengthening.Furthermore,the precipitation strengthening of γ" and γ" phases induces the increase of flow stress.An important conclusion is drawn that the size effect at the mesoscopic scale depends not only on the sample size and grain size but also on the particle size and volume fraction of the precipitates.The established constitutive model which considers grain boundary strengthening,precipitation strengthening and solid solution strengthening can accurately describe the flow stress characteristics of nickel-based superalloys at the mesoscopic scale.
基金the financial support of the project from the National Natural Science Foundation of China(Grant No.51575129)Key Research and Development Program of Shandong Province(Grant No.2016GGX102026)the Training Program of the Major Research Plan of the National Natural Science Foundation of China(Grant No.91860129).
文摘Nickel-based superalloys have become the key materials of micro-parts depending on excellent mechanical properties at high temperatures.The plastic deformation behavior is difficult to predict due to the occurrence of size effect on the mesoscopic scale.In this paper,the effect of specimen diameter to grain size ratio(D/d)on the flow stress and inhomogeneous plastic deformation behavior in compression of nickel-based superalloy cylindrical specimens was investigated on the mesoscopic scale.The results showed that when D/d is less than 9.7,the flow stress increases with the grain size.Aiming at this phenomenon,a flow stress size effect model considering compressive strain partitioning was established.The calculated flow stress values agree well with the experimental values,thus revealing the effect of D/d on the flow stress in compression of nickel-base superalloy on the mesoscopic scale.The inhomogeneous plastic deformation during compression deformation increases with the grain size.The end surface profiles evolve from a regular circular shape to an irregular shape with the grain size.The surface folding phenomenon occurs only in partially compressed specimen with a few grains across the diameter.Crystal plasticity finite-element(CPFE)simulation of compression deformation on the mesoscopic scale real-time displayed the evolution of microstructure.The study of this paper has important guiding significance for understanding the influence of D/d on the compression deformation behavior of nickel-based superalloy on the mesoscopic scale.
基金The authors would like to acknowledge the financial support from the National Natural Science Foundation of China(Grant Nos.92163210,11674108,52002134)Funding by Science and Technology Projects in Guangzhou(202201000008)+1 种基金the Science and Technology Planning Project of Guangdong Province(No.2019KQNCX028)the Natural Science Foundation of South China Normal University(No.19KJ01)。
文摘Effective tuning of nanoscale domain structures provides fundamental basis for controlling and engineering of various functionalities in ferroelectric materials.In this work,we demonstrate the precise patterning of nanoscopic domain structures in as-grown epitaxial PbTiO_(3)(PTO)films by merely introducing an ultrathin pre-patterned doping layer(e.g.,Fe-doped PTO).The doping layer can effectively reverse the interfacial built-in bias,consequent to a reversed initial polarization reorientation in the as-grown film,which makes it possible to transfer the nano-patterns in the doping layer into the domain structure of ferroelectric films.For instance,we have successfully fabricated large area ordered array of nanoscale cylindrical domains(downward polarization)embedded in the matrix domain with opposite polarization(upward polarization)in PTO film.These nanoscale cylinder domains also allow deterministic and reversible erasure and creation induced by biased tip scanning.The results provide an effective pathway for on-demand patterning of large area nanoscale domains in the as-grown films,which may find applications in a wide range of nanoelectronic devices.
文摘The sulphide ores of the Baimazhai deposit, although typically orthomagmatic, locally exhibit peculiar textural features and are intimately associated with hydrothermal minerals, such as biotite, amphibole and chlorite. This association suggests that the magmatic sulphide ores were subjected to hydrothermal alteration and subsequent redistribution, resulting in the observed textural features. Geochemically, the Baimazhai sulphide ores are enriched in Cu, Pd and Au, which,according to previous studies, reflects the action of hydrothermal fluids. Interestingly, Ar-Ar dating yielded the plateau ages of about 160–170 Ma, which are at odds with the established Permian age of the Emeishan large igneous province. We interpreted these younger ages as due to thermal resetting during post-Permian tectonothermal events. We have proposed a model in which tectonic movements and hydrothermal fluids related to these events modified the pre-existing magmatic sulphides. Given the degree of overprint, we suggested two possible scenarios: 1) the sulphide disseminations that surround the massive magmatic ores are the result of deformation and hydrothermal alteration; and 2) there were both magmatic massive and disseminated sulphides, in which case the scale and relocation of remobilization would have been smaller, but still detectable.
