The injury tolerance of cell plasma membrane and the correlative enzymes activities of plasma-membrane protection system in the Ulmuspumila leaves treated by nine concentrations （0.3%, 0.6%, 0,9%, 1,2%, 1.5%, 1.8%, 2...The injury tolerance of cell plasma membrane and the correlative enzymes activities of plasma-membrane protection system in the Ulmuspumila leaves treated by nine concentrations （0.3%, 0.6%, 0,9%, 1,2%, 1.5%, 1.8%, 2,1%, 2.4%, 3.0%） of Na2CO3 and NaHCO3 mixtures were studied in a greenhouse of Northeast Forestry University, Harbin, China. The rate of electrolyte leakage （REL） and SOD （Superoxide dismutase） activity in leaves of different samples were determined. Results showed that the REL in leaves of U. pumila presented a slowly increasing trend at the salt concentrations less than 1.5%, which indicated that cell plasma membrane of U. pumila leaves had rather strong resistance to the injury of salt ion, and had a significant increase at the salt concentrations more than 1.5%. The SOD activities in leaves of U, pumila presented an increased trend at salt concentrations less than 1.5%, the growth of seedlings did not decline, and tress and leaves had no symptom of injury, while the salt concentrations exceeded 1.5%, SOD activities sharply decreased and REL increased promptly.展开更多
Deformation and vibration of twig-connected sin- gle leaf in wind is investigated experimentally. Results show that the Reynolds number based on wind speed and length of leaf blade is a key parameter to the aerodynami...Deformation and vibration of twig-connected sin- gle leaf in wind is investigated experimentally. Results show that the Reynolds number based on wind speed and length of leaf blade is a key parameter to the aerodynamic prob- lem. In case the front surface facing the wind and with an in- crease of Reynolds number, the leaf experiences static defor- mation, large amplitude and low frequency sway, reconfigu- ration to delta wing shape, flapping of tips, high frequency vibration of whole leaf blade, recovery of delta wing shape, and twig-leaf coupling vibration. Abrupt changes from one state to another occur at critical Reynolds numbers. In case the back surface facing the wind, the large amplitude and low frequency sway does not occur, the recovered delta wing shape is replaced by a conic shape, and the critical Reynolds numbers of vibrations are higher than the ones correspond- ing to the case with the front surface facing the wind. A pair of ram-horn vortex is observed behind the delta wing shaped leaf. A single vortex is found downstream of the conic shaped leaf. A lift is induced by the vortex, and this lift helps leaf to adjust position and posture, stabilize blade distortion and reduce drag and vibration.展开更多
The role of late embryogenesis abundant （LEA） proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezi...The role of late embryogenesis abundant （LEA） proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezing stresses and additional tolerance to heat, NaHCO3 （salt-alkali） and ultraviolet radiation was also investigated. The transgenic yeast harboring the Tamarix LEA gene （DQ663481） was generated under the control of inducible GAL promoter （pYES2 vector）, yeast cells transformed with pYES2 empty vector were also generated as a control. Stress tolerance tests showed that LEA yeast transformants exhibited a higher survival rates than the control transformants under high temperature, NaHCO3, ultraviolet radiation, salt （NaCl）, drought and freezing, indicating that the LEA gene is tolerant to these abiotic stresses. These results suggest that the LEA gene is resistant to a wider repertoire of stresses and may play a common role in plant acclimation to the examined stress conditions.展开更多
The basic method of DNA extraction （CTAB） was improved as the multi-times STE-CTAB extraction method and used to extract the DNA of birch leaved in this experiment. Results showed that the improved method is suitabl...The basic method of DNA extraction （CTAB） was improved as the multi-times STE-CTAB extraction method and used to extract the DNA of birch leaved in this experiment. Results showed that the improved method is suitable not only for genomic DNA extraction of birch but also for that of other plants. The purity of genornic DNA extracted by the.multi-times STE-CTAB extraction method is higher than that by one time STE-CTAB method, and it does not need the process of RNase. The factors of influencing ISSR system were explored based on the genomic DNA of birch extracted by the two methods. The optimal conditions for ISSR system were determined as follows： Mg2＋ concentration is 1.5-3.0 mmol·L^-1, dNTP concentration 0.104）.25 mmol·L^-1, the quantity of Taq polymerase 0.5-2.0 U, template DNA 30-100 ng, and the concentration of primer is 0.2-0.4 pmmol·L^-1, and the reaction program was as： initial denaturation for 5 min at 94℃, 30 cycles of denaturation for 30 s at 94℃,annealing for 30 s at 51℃, extension for 30 s at 72℃, and a final 7 min extension at 72℃.展开更多
In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cere...In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography.At each measured time point,the total photoacoustic signal was significantly higher on the affected side than on the healthy side.Twelve hours after reperfusion,cerebral perfusion on the affected side increased,cerebrovascular injury worsened,and anti-tropomyosin 4 expression increased.Twenty-four hours after reperfusion and later,perfusion on the affected side declined slowly and stabilized after 1 week;brain injury was also alleviated.Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes.The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression.展开更多
Dear Editor,Electroporation(EP)is a non-viral method for introducing macromolecules such as DNA,protein,and messenger RNA(mRNA)into mammalian cells for both basic research and ex vivo therapy(Glass et al.,2018).Due to...Dear Editor,Electroporation(EP)is a non-viral method for introducing macromolecules such as DNA,protein,and messenger RNA(mRNA)into mammalian cells for both basic research and ex vivo therapy(Glass et al.,2018).Due to its nature,EP offers great promise as a delivery method for gene therapy without causing risks associated with the viral vector-mediated delivery(Yin et al.,2017;Qiu et al.,2022).展开更多
Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE te...Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE technique. The sequence analysis showed the open reading frame （ORF） of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein （GFP） marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase.展开更多
Thrombospondins （TSPs） are a family of multidomain extracellular matrix glycoproteins, which consist offive members at present, TSP-1, -2, -3, -4 and TSP-5. TSP-1, which is synthesized and secreted by activated plat...Thrombospondins （TSPs） are a family of multidomain extracellular matrix glycoproteins, which consist offive members at present, TSP-1, -2, -3, -4 and TSP-5. TSP-1, which is synthesized and secreted by activated platelets and a variety of cell types including endothelial cells, macrophages, monotypes, fibroblasts, tumor cells and vascular smooth muscular cells, is the best characterized family member./ TSP-1 participates in a wide range of biological responses, including platelet activation and aggregation, vascular smooth muscular cell proliferation and migration, endothelial cell apoptosis and angiogenesis.l-3 TSP-1 may be involved in the pathogenesis of atherosclerosis and coronary artery disease （CAD）/myocardial infarction （MI）.4-7展开更多
AIM:To characterise expression of interleukin 6(IL-6),a potent proinflammatory cytokine,in the occurrence and development of inflammatory bowel disease(IBD) and investigate its effect on neuroimmunomodulation and immu...AIM:To characterise expression of interleukin 6(IL-6),a potent proinflammatory cytokine,in the occurrence and development of inflammatory bowel disease(IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis regulation.METHODS:In this study,rats with colitis induced by trinitrobenzene sulfonic acid(TNBS) were sacrificed on days 3,7,14,21 and 28 after induction.In the controls,the TNBS was just replaced by equivalent amount of phosphate buffered solution(PBS,0.01 mol/L).IL-6 mRNA expression in brain and colon tissues in each phase was evaluated by real-time reverse transcriptionpolymerase chain reaction,and cellular localisation and protein level of IL-6 was determined by immunohistochemistry.RESULTS:At day 7,mRNA expression of IL-6 was significantly higher in the colon and brain of IBD rats than that of the controls.The protein level was also significantly higher in colon,hypothalamus and cerebral cortex of IBD rats compared with the controls.So there are similar temporal trends in IL-6 mRNA expression and protein levels in all positions with a persistent increase to a peak at day 7,followed by a decline and gradual return to normal levels.CONCLUSION:These results revealed that changes in IL-6 expression in brain and colon tissues occur in different phases of IBD.Therefore,we propose that the nerve centre regulates and controls the occurrence and development of IBD via IL-6.展开更多
The electrochemical oxidation degradation processes for artificial and actual wastewater containing ammonia were carried out with a Ti/RuO2-Pt anode and a Ti plate cathode. We studied the effects of different current ...The electrochemical oxidation degradation processes for artificial and actual wastewater containing ammonia were carried out with a Ti/RuO2-Pt anode and a Ti plate cathode. We studied the effects of different current densities, space sizes between the two electrodes, and amounts of added NaCl on ammonia-containing wastewater treatm.ent. It was shown that, after a 30-min treatment under the optimal conditions, which were a current density of 20 mA/cm2, a space size between the two electrodes of I cm, and an added amount of 0.5 g/L of NaC1, the COD concentration in municipal wastewater was 40 mg/L, a removal rate of 90%; and the NH3-N concentration was 7 mg/L, a removal rate of 88.3%. The effluent of municipal wastewater qualified for Class A of the Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant （GB 18918-2002）.展开更多
The impact and solidification processes of single melting tin dust at the micron scale on a spherical bead were numerically studied with hot flue gas flow. The geometrical evolution of dust impacting on hot bead and s...The impact and solidification processes of single melting tin dust at the micron scale on a spherical bead were numerically studied with hot flue gas flow. The geometrical evolution of dust impacting on hot bead and spreading without solidification involved initial spreading, retraction and oscillation, and stabilizing. The increased impact angle was found to reduce maximum spread area, weaken retraction and oscillation, and raise steady spread area. Dust impacting on cold bead completely solidified after liquid spreading and solidification without retraction and oscillation. Increased impact angle raised solidification sliding distance, whereas it reduced solidification spread area. Then, the effects of bead temperature, dust inlet velocity and size on the sliding and spreading of dust were studied, and the results indicated that increasing bead temperature, dust inlet velocity and size could raise solidification sliding distance and solidification spread area. With the dusts continually impacting on the bed, a dust layer forms at the front of bead, being different from that of solid dust, which becomes thick firstly, and then spreads from bead front to sides.展开更多
Based on the refined dynamic equation of stretching plates, the elastic tensio compression wave scattering and dynamic stress concentrations in the thick plate with two cutouts are studied. In view of the problem that...Based on the refined dynamic equation of stretching plates, the elastic tensio compression wave scattering and dynamic stress concentrations in the thick plate with two cutouts are studied. In view of the problem that the shear stress is automatically satisfied under the free boundary condition, the generalized stress of the first-order vanishing moment of shear stress is considered. The numerical results indicate that, as the cutout is thick, the maximum value of the dynamic stress factor obtained using the refined dynamic theory is 19% higher than that from the solution of plane stress problems of elastic dynamics.展开更多
BACKGROUND Aggressive vertebral hemangioma(VH)is an uncommon lesion in the adult population.The vast majority of aggressive VHs have typical radiographic features.However,preoperative diagnosis of atypical aggressive ...BACKGROUND Aggressive vertebral hemangioma(VH)is an uncommon lesion in the adult population.The vast majority of aggressive VHs have typical radiographic features.However,preoperative diagnosis of atypical aggressive VH may be difficult.Aggressive VHs are likely to recur even with en bloc resection.CASE SUMMARY A 52-year-old woman presented with a 3-mo history of numbness and pain in her right lower extremity.Physical examination showed sacral tenderness and limited mobility,and the muscle strength was grade 4 in the right digital flexor.Computed tomography revealed osteolytic bone destruction from S1 to S2.Magnetic resonance imaging(MRI)showed that the mass was compressing the dural sac;it was heterogeneously hypointense on T1-weighted MRI and hyperintense on T2-weighted MRI,and gadolinium contrast enhancement showed that the tumor was heterogeneously enhanced and invading the vertebral endplate of S1.The patient developed progressive back pain and numbness in the bilateral extremities 6 mo postoperatively,and MRI examination showed recurrence of the mass.The mass was larger in size than before the operation,and it was extending into the spinal canal.CONCLUSION The radiographic findings of atypical aggressive VH include osteolytic vertebral bone destruction,extension of the mass into the spinal canal,and heterogeneous signal intensity on T1-,T2-,and enhanced T1-weighted MRI.These characteristics make preoperative diagnosis difficult,and biopsy is necessary to verify the lesion.Surgical decompression and gross total resection are recommended for treatment of aggressive VH.However,recurrence is inevitable in some cases.展开更多
BACKGROUND Periosteal chondroma is a very rare benign tumor that develops adjacent to the cortical surface of bone and beneath the periosteal membrane.Periosteal chondroma of the rib is an extremely rare entity.CASE S...BACKGROUND Periosteal chondroma is a very rare benign tumor that develops adjacent to the cortical surface of bone and beneath the periosteal membrane.Periosteal chondroma of the rib is an extremely rare entity.CASE SUMMARY The patient was a 43-year-old man who had been incidentally found to have a mediastinal mass 1 mo earlier.Plain computed tomography showed a lobulated soft tissue mass on the right side of the T4/5 vertebra that measured about 47 mm×28 mm in the transverse view and contained diffuse stippled calcification.The mass caused cortical scalloping of the right fourth rib and marginal bone sclerosis.Enhanced computed tomography showed mild enhancement of the mass.Magnetic resonance imaging showed a lobulated mass on the right side of the thoracic vertebra with long TI and T2 signals,mottling,and patchy long T1 and short T2 signals inside.The lesion had a hypointense rim.Enhanced magnetic resonance imaging showed enhancement predominantly at the periphery of the tumor.The tumor was approached through a right posterolateral thoracotomy,and parts of the fourth and fifth ribs were excised with the tumor.Postoperative pathological analysis revealed periosteal chondroma of the rib.CONCLUSION Periosteal chondroma of the rib has a low incidence and typical imaging manifestations.Understanding its imaging features is helpful to ensure a correct preoperative diagnosis.展开更多
Objective： Cervical cancer （CC） is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses （HR-HPVs） are considered to be the major causes of cervical cance...Objective： Cervical cancer （CC） is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses （HR-HPVs） are considered to be the major causes of cervical cancer. HPV16 is the most common type of HR-HPVs and HPV16 E6 gene is one of the major oncogenes. Specific mutations are considered as dangerous factors causing CC. This study was designed to find mutations of HPV16 E6 and the relationship between the mutations and the happening of CC. Methods： The tissue DNA was extracted from 15 biopsies of CC. Part of HPV16 E6 gene （nucleotide 201-523） was amplified by polymerase chain reaction （PCR） from the CC tissue DNA. The PCR fragments were sequenced and analyzed. Results： The result of PCR showed that the positive rate of HPV16 E6 was 93.33% （14/13）. After sequencing ana analyzing, in the 13 out of 14 PCR fragments, 4 maintained prototype （30.77%）, 8 had a same 350G mutation （61.54%）, and 1 had a 249G mutation （7.69%）. Conclusion： This study suggest that there is a high infection rate of HPV in cervical cancer and most of the HPV16 E6 gene has mutations. Those mutations may have an association with the development of cervical cancer.展开更多
BACKGROUND Multiple myeloma is a malignant hematological disease characterized by proliferation of monoclonal plasma cells mainly in the bone marrow.Extraosseous epidural plasmacytoma associated with myeloma arises fr...BACKGROUND Multiple myeloma is a malignant hematological disease characterized by proliferation of monoclonal plasma cells mainly in the bone marrow.Extraosseous epidural plasmacytoma associated with myeloma arises from lymphoid tissue in the epidural space without focal vertebral involvement,and is rare.CASE SUMMARY A 52-year-old woman was diagnosed with kappa subtype nonsecretory multiple myeloma and presented with bilateral arm weakness 11 mo after completing multiple courses of chemotherapy.Spinal magnetic resonance imaging(MRI)showed a posterior C7–T3 epidural mass with spinal cord compression.After five courses of chemotherapy,follow-up MRI showed resolution of cord compression.A 54-year-old man presented with paraplegia 15 mo after a diagnosis of IgD kappa subtype multiple myeloma and completing multiple courses of chemotherapy.He underwent Th11 and L1 laminectomies for tumor resection because MRI showed an epidural mass causing cord compression.Histopathologic examination was consistent with IgD multiple myeloma.The patients have currently survived for 33 mo and 19 mo,respectively.CONCLUSION Isolated extraosseous epidural plasmacytoma associated with multiple myeloma without bony involvement is difficult to diagnose by imaging.Definitive diagnosis requires pathological and immunohistochemical examination.展开更多
The expression of Selenoprotein W(SelW)in C2C12 skeletal muscle cells was specifically decreased to examine its influence on the amount of glutathione(GSH)and the activity of glutathione peroxidase(GPx).SelW knock-dow...The expression of Selenoprotein W(SelW)in C2C12 skeletal muscle cells was specifically decreased to examine its influence on the amount of glutathione(GSH)and the activity of glutathione peroxidase(GPx).SelW knock-down was performed by RNA interference(RNAi)in cultured muscle cells and verified by Real-time PCR and Western blotting.In addition,cell viability,GSH content and GPx activity were assayed.The results showed that the mRNA level and protein expression of SelW were decreased successfully by 71.9%and 68.8%relative to control values,cell viability decreased by 21.5%,GSH increased by 29.76%,and GPx increased by 47.58%.WST assay showed that compared with blank control,the value of positive group dropped 21.5%;In GSH and GPx assay,compared with blank control the positive group increased29.76%and 47.58%separately.In conclusion,SelW knock-down by RNAi caused significant cytotoxity in skeletal muscle cells and led to compensatory increases in GSH content and GPx activity.These findings are consistent with the suggestions from bioinformatics indicating an antioxidative role for SelW in skeletal muscle cells.展开更多
文摘The injury tolerance of cell plasma membrane and the correlative enzymes activities of plasma-membrane protection system in the Ulmuspumila leaves treated by nine concentrations （0.3%, 0.6%, 0,9%, 1,2%, 1.5%, 1.8%, 2,1%, 2.4%, 3.0%） of Na2CO3 and NaHCO3 mixtures were studied in a greenhouse of Northeast Forestry University, Harbin, China. The rate of electrolyte leakage （REL） and SOD （Superoxide dismutase） activity in leaves of different samples were determined. Results showed that the REL in leaves of U. pumila presented a slowly increasing trend at the salt concentrations less than 1.5%, which indicated that cell plasma membrane of U. pumila leaves had rather strong resistance to the injury of salt ion, and had a significant increase at the salt concentrations more than 1.5%. The SOD activities in leaves of U, pumila presented an increased trend at salt concentrations less than 1.5%, the growth of seedlings did not decline, and tress and leaves had no symptom of injury, while the salt concentrations exceeded 1.5%, SOD activities sharply decreased and REL increased promptly.
基金supported by the National Natural Science Foundation of China (10872188 and 11172286)
文摘Deformation and vibration of twig-connected sin- gle leaf in wind is investigated experimentally. Results show that the Reynolds number based on wind speed and length of leaf blade is a key parameter to the aerodynamic prob- lem. In case the front surface facing the wind and with an in- crease of Reynolds number, the leaf experiences static defor- mation, large amplitude and low frequency sway, reconfigu- ration to delta wing shape, flapping of tips, high frequency vibration of whole leaf blade, recovery of delta wing shape, and twig-leaf coupling vibration. Abrupt changes from one state to another occur at critical Reynolds numbers. In case the back surface facing the wind, the large amplitude and low frequency sway does not occur, the recovered delta wing shape is replaced by a conic shape, and the critical Reynolds numbers of vibrations are higher than the ones correspond- ing to the case with the front surface facing the wind. A pair of ram-horn vortex is observed behind the delta wing shaped leaf. A single vortex is found downstream of the conic shaped leaf. A lift is induced by the vortex, and this lift helps leaf to adjust position and posture, stabilize blade distortion and reduce drag and vibration.
基金National Key Program on Basic Research and Development of China (G1999016003)
文摘The role of late embryogenesis abundant （LEA） proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezing stresses and additional tolerance to heat, NaHCO3 （salt-alkali） and ultraviolet radiation was also investigated. The transgenic yeast harboring the Tamarix LEA gene （DQ663481） was generated under the control of inducible GAL promoter （pYES2 vector）, yeast cells transformed with pYES2 empty vector were also generated as a control. Stress tolerance tests showed that LEA yeast transformants exhibited a higher survival rates than the control transformants under high temperature, NaHCO3, ultraviolet radiation, salt （NaCl）, drought and freezing, indicating that the LEA gene is tolerant to these abiotic stresses. These results suggest that the LEA gene is resistant to a wider repertoire of stresses and may play a common role in plant acclimation to the examined stress conditions.
基金This paper was supported by National Natural Science Foundation of China (No. 30571513) and National High Technology Research and Development Program of China (863 Program) (No. 2002AA241080).
文摘The basic method of DNA extraction （CTAB） was improved as the multi-times STE-CTAB extraction method and used to extract the DNA of birch leaved in this experiment. Results showed that the improved method is suitable not only for genomic DNA extraction of birch but also for that of other plants. The purity of genornic DNA extracted by the.multi-times STE-CTAB extraction method is higher than that by one time STE-CTAB method, and it does not need the process of RNase. The factors of influencing ISSR system were explored based on the genomic DNA of birch extracted by the two methods. The optimal conditions for ISSR system were determined as follows： Mg2＋ concentration is 1.5-3.0 mmol·L^-1, dNTP concentration 0.104）.25 mmol·L^-1, the quantity of Taq polymerase 0.5-2.0 U, template DNA 30-100 ng, and the concentration of primer is 0.2-0.4 pmmol·L^-1, and the reaction program was as： initial denaturation for 5 min at 94℃, 30 cycles of denaturation for 30 s at 94℃,annealing for 30 s at 51℃, extension for 30 s at 72℃, and a final 7 min extension at 72℃.
基金supported by the National Natural Science Foundation of China,No.81730050(to WH).
文摘In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography.At each measured time point,the total photoacoustic signal was significantly higher on the affected side than on the healthy side.Twelve hours after reperfusion,cerebral perfusion on the affected side increased,cerebrovascular injury worsened,and anti-tropomyosin 4 expression increased.Twenty-four hours after reperfusion and later,perfusion on the affected side declined slowly and stabilized after 1 week;brain injury was also alleviated.Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes.The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression.
基金supported by the National Key Research and Development Program of China(2022YFF1002801 to Y.Z.,2019YFA0802801 and 2018YFA0801401 to H.Y.)the National Natural Science Foundation of China(31972936 to Y.Z.)+3 种基金Medical Science Advancement Program(Basic Medical Sciences)of Wuhan University(TFJC2018005)the Fundamental Research Funds for the Central Universities(to Y.Z.)the startup funding from Wuhan University(to Y.Z.)Y.Z.,H.Y.,C.-P.Z.,and J.A.have filed a patent application on buffer composition through Wuhan University(PCT International Application No.PCT/CN2022/106565)。
文摘Dear Editor,Electroporation(EP)is a non-viral method for introducing macromolecules such as DNA,protein,and messenger RNA(mRNA)into mammalian cells for both basic research and ex vivo therapy(Glass et al.,2018).Due to its nature,EP offers great promise as a delivery method for gene therapy without causing risks associated with the viral vector-mediated delivery(Yin et al.,2017;Qiu et al.,2022).
基金supported by the Heilongjiang Province Postdoctoral Science Foundation(LBH-Q10144)
文摘Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE technique. The sequence analysis showed the open reading frame （ORF） of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein （GFP） marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase.
文摘Thrombospondins （TSPs） are a family of multidomain extracellular matrix glycoproteins, which consist offive members at present, TSP-1, -2, -3, -4 and TSP-5. TSP-1, which is synthesized and secreted by activated platelets and a variety of cell types including endothelial cells, macrophages, monotypes, fibroblasts, tumor cells and vascular smooth muscular cells, is the best characterized family member./ TSP-1 participates in a wide range of biological responses, including platelet activation and aggregation, vascular smooth muscular cell proliferation and migration, endothelial cell apoptosis and angiogenesis.l-3 TSP-1 may be involved in the pathogenesis of atherosclerosis and coronary artery disease （CAD）/myocardial infarction （MI）.4-7
基金Supported by The grant from the National Natural Science Foundation of China,No 30871840No 30901057
文摘AIM:To characterise expression of interleukin 6(IL-6),a potent proinflammatory cytokine,in the occurrence and development of inflammatory bowel disease(IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis regulation.METHODS:In this study,rats with colitis induced by trinitrobenzene sulfonic acid(TNBS) were sacrificed on days 3,7,14,21 and 28 after induction.In the controls,the TNBS was just replaced by equivalent amount of phosphate buffered solution(PBS,0.01 mol/L).IL-6 mRNA expression in brain and colon tissues in each phase was evaluated by real-time reverse transcriptionpolymerase chain reaction,and cellular localisation and protein level of IL-6 was determined by immunohistochemistry.RESULTS:At day 7,mRNA expression of IL-6 was significantly higher in the colon and brain of IBD rats than that of the controls.The protein level was also significantly higher in colon,hypothalamus and cerebral cortex of IBD rats compared with the controls.So there are similar temporal trends in IL-6 mRNA expression and protein levels in all positions with a persistent increase to a peak at day 7,followed by a decline and gradual return to normal levels.CONCLUSION:These results revealed that changes in IL-6 expression in brain and colon tissues occur in different phases of IBD.Therefore,we propose that the nerve centre regulates and controls the occurrence and development of IBD via IL-6.
基金supported by the Eleventh Five-Year Key Technology Research and Development Program in China (Grant No. 2006BAJ08B04)
文摘The electrochemical oxidation degradation processes for artificial and actual wastewater containing ammonia were carried out with a Ti/RuO2-Pt anode and a Ti plate cathode. We studied the effects of different current densities, space sizes between the two electrodes, and amounts of added NaCl on ammonia-containing wastewater treatm.ent. It was shown that, after a 30-min treatment under the optimal conditions, which were a current density of 20 mA/cm2, a space size between the two electrodes of I cm, and an added amount of 0.5 g/L of NaC1, the COD concentration in municipal wastewater was 40 mg/L, a removal rate of 90%; and the NH3-N concentration was 7 mg/L, a removal rate of 88.3%. The effluent of municipal wastewater qualified for Class A of the Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant （GB 18918-2002）.
文摘The impact and solidification processes of single melting tin dust at the micron scale on a spherical bead were numerically studied with hot flue gas flow. The geometrical evolution of dust impacting on hot bead and spreading without solidification involved initial spreading, retraction and oscillation, and stabilizing. The increased impact angle was found to reduce maximum spread area, weaken retraction and oscillation, and raise steady spread area. Dust impacting on cold bead completely solidified after liquid spreading and solidification without retraction and oscillation. Increased impact angle raised solidification sliding distance, whereas it reduced solidification spread area. Then, the effects of bead temperature, dust inlet velocity and size on the sliding and spreading of dust were studied, and the results indicated that increasing bead temperature, dust inlet velocity and size could raise solidification sliding distance and solidification spread area. With the dusts continually impacting on the bed, a dust layer forms at the front of bead, being different from that of solid dust, which becomes thick firstly, and then spreads from bead front to sides.
基金supported by the Natural Science Foundation of Zhejiang Province of China (Grant No. LQ17E050011)the National Natural Science Foundation of China (Grant No. 51775154)+1 种基金the Natural Science Foundation of Zhejiang Province of China (Grant No.LQ17E090007)the Key Project of Natural Science Foundation of Zhejiang Province of China (Grant No. LQ17E050011)
文摘Based on the refined dynamic equation of stretching plates, the elastic tensio compression wave scattering and dynamic stress concentrations in the thick plate with two cutouts are studied. In view of the problem that the shear stress is automatically satisfied under the free boundary condition, the generalized stress of the first-order vanishing moment of shear stress is considered. The numerical results indicate that, as the cutout is thick, the maximum value of the dynamic stress factor obtained using the refined dynamic theory is 19% higher than that from the solution of plane stress problems of elastic dynamics.
文摘BACKGROUND Aggressive vertebral hemangioma(VH)is an uncommon lesion in the adult population.The vast majority of aggressive VHs have typical radiographic features.However,preoperative diagnosis of atypical aggressive VH may be difficult.Aggressive VHs are likely to recur even with en bloc resection.CASE SUMMARY A 52-year-old woman presented with a 3-mo history of numbness and pain in her right lower extremity.Physical examination showed sacral tenderness and limited mobility,and the muscle strength was grade 4 in the right digital flexor.Computed tomography revealed osteolytic bone destruction from S1 to S2.Magnetic resonance imaging(MRI)showed that the mass was compressing the dural sac;it was heterogeneously hypointense on T1-weighted MRI and hyperintense on T2-weighted MRI,and gadolinium contrast enhancement showed that the tumor was heterogeneously enhanced and invading the vertebral endplate of S1.The patient developed progressive back pain and numbness in the bilateral extremities 6 mo postoperatively,and MRI examination showed recurrence of the mass.The mass was larger in size than before the operation,and it was extending into the spinal canal.CONCLUSION The radiographic findings of atypical aggressive VH include osteolytic vertebral bone destruction,extension of the mass into the spinal canal,and heterogeneous signal intensity on T1-,T2-,and enhanced T1-weighted MRI.These characteristics make preoperative diagnosis difficult,and biopsy is necessary to verify the lesion.Surgical decompression and gross total resection are recommended for treatment of aggressive VH.However,recurrence is inevitable in some cases.
文摘BACKGROUND Periosteal chondroma is a very rare benign tumor that develops adjacent to the cortical surface of bone and beneath the periosteal membrane.Periosteal chondroma of the rib is an extremely rare entity.CASE SUMMARY The patient was a 43-year-old man who had been incidentally found to have a mediastinal mass 1 mo earlier.Plain computed tomography showed a lobulated soft tissue mass on the right side of the T4/5 vertebra that measured about 47 mm×28 mm in the transverse view and contained diffuse stippled calcification.The mass caused cortical scalloping of the right fourth rib and marginal bone sclerosis.Enhanced computed tomography showed mild enhancement of the mass.Magnetic resonance imaging showed a lobulated mass on the right side of the thoracic vertebra with long TI and T2 signals,mottling,and patchy long T1 and short T2 signals inside.The lesion had a hypointense rim.Enhanced magnetic resonance imaging showed enhancement predominantly at the periphery of the tumor.The tumor was approached through a right posterolateral thoracotomy,and parts of the fourth and fifth ribs were excised with the tumor.Postoperative pathological analysis revealed periosteal chondroma of the rib.CONCLUSION Periosteal chondroma of the rib has a low incidence and typical imaging manifestations.Understanding its imaging features is helpful to ensure a correct preoperative diagnosis.
基金supported by a grant from the 11th Five-year Plan Medical Science Fundation of PLA (No06MA084)
文摘Objective： Cervical cancer （CC） is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses （HR-HPVs） are considered to be the major causes of cervical cancer. HPV16 is the most common type of HR-HPVs and HPV16 E6 gene is one of the major oncogenes. Specific mutations are considered as dangerous factors causing CC. This study was designed to find mutations of HPV16 E6 and the relationship between the mutations and the happening of CC. Methods： The tissue DNA was extracted from 15 biopsies of CC. Part of HPV16 E6 gene （nucleotide 201-523） was amplified by polymerase chain reaction （PCR） from the CC tissue DNA. The PCR fragments were sequenced and analyzed. Results： The result of PCR showed that the positive rate of HPV16 E6 was 93.33% （14/13）. After sequencing ana analyzing, in the 13 out of 14 PCR fragments, 4 maintained prototype （30.77%）, 8 had a same 350G mutation （61.54%）, and 1 had a 249G mutation （7.69%）. Conclusion： This study suggest that there is a high infection rate of HPV in cervical cancer and most of the HPV16 E6 gene has mutations. Those mutations may have an association with the development of cervical cancer.
文摘BACKGROUND Multiple myeloma is a malignant hematological disease characterized by proliferation of monoclonal plasma cells mainly in the bone marrow.Extraosseous epidural plasmacytoma associated with myeloma arises from lymphoid tissue in the epidural space without focal vertebral involvement,and is rare.CASE SUMMARY A 52-year-old woman was diagnosed with kappa subtype nonsecretory multiple myeloma and presented with bilateral arm weakness 11 mo after completing multiple courses of chemotherapy.Spinal magnetic resonance imaging(MRI)showed a posterior C7–T3 epidural mass with spinal cord compression.After five courses of chemotherapy,follow-up MRI showed resolution of cord compression.A 54-year-old man presented with paraplegia 15 mo after a diagnosis of IgD kappa subtype multiple myeloma and completing multiple courses of chemotherapy.He underwent Th11 and L1 laminectomies for tumor resection because MRI showed an epidural mass causing cord compression.Histopathologic examination was consistent with IgD multiple myeloma.The patients have currently survived for 33 mo and 19 mo,respectively.CONCLUSION Isolated extraosseous epidural plasmacytoma associated with multiple myeloma without bony involvement is difficult to diagnose by imaging.Definitive diagnosis requires pathological and immunohistochemical examination.
基金supported by China Postdoctoral Science Foundation(LRB06-262)Funds of Northeast Forestry University
文摘The expression of Selenoprotein W(SelW)in C2C12 skeletal muscle cells was specifically decreased to examine its influence on the amount of glutathione(GSH)and the activity of glutathione peroxidase(GPx).SelW knock-down was performed by RNA interference(RNAi)in cultured muscle cells and verified by Real-time PCR and Western blotting.In addition,cell viability,GSH content and GPx activity were assayed.The results showed that the mRNA level and protein expression of SelW were decreased successfully by 71.9%and 68.8%relative to control values,cell viability decreased by 21.5%,GSH increased by 29.76%,and GPx increased by 47.58%.WST assay showed that compared with blank control,the value of positive group dropped 21.5%;In GSH and GPx assay,compared with blank control the positive group increased29.76%and 47.58%separately.In conclusion,SelW knock-down by RNAi caused significant cytotoxity in skeletal muscle cells and led to compensatory increases in GSH content and GPx activity.These findings are consistent with the suggestions from bioinformatics indicating an antioxidative role for SelW in skeletal muscle cells.
