Objective： To compare the chemical characters of Sparganii Rhizoma from different areas via chromatographic analysis and to establish a sensitive LC/MS method for quality assessment of Sparganii Rhizoma.Methods： Und...Objective： To compare the chemical characters of Sparganii Rhizoma from different areas via chromatographic analysis and to establish a sensitive LC/MS method for quality assessment of Sparganii Rhizoma.Methods： Under the optimised HPLC-PDA chromatographic conditions,twenty batches of Sparganii Rhizoma were analyzed by HPLC fingerprints.Principal component analysis（PCA）,orthogonal projections to latent structures discriminant analysis（OPLS-DA）and hierarchical cluster analysis（HCA）were performed based on all peak areas of Sparganii Rhizoma fingerprints.Meanwhile,part of common peaks were subsequently quantified by UFLC-QTRAP-MS.Results： The similarity values of HPLC fingerprints fluctuated in a wide range of 0.511–0.973,which showed variable differences of chemical characters among Sparganii Rhizoma from twenty habitats.PCA,OPLS-DA and HCA indicated that samples could be divided into five groups with different chemical characters,which generally corresponded with their geographical distributions.A total of 31 peaks in HPLC fingerprints were marked,and eight of them were identified and quantified.The quantitative result was generally in agreement with the classifications based on HPLC fingerprints,which indicated that Sparganii Rhizoma samples from eastern China mostly contained more contents including phenolic acids and flavonoids.Conclusion： This study not only proved that there were relationships between geographic distributions and internal chemical compositions of plants,which could provide evidence to the traditional Chinese medicine concept ＂geo-authentic＂,but also supplied a sensitive and rapid simultaneous quantitive method for the quality estimation of Sparganii Rhizoma.展开更多
艾绒质量是影响灸热疗效的关键因素,传统艾绒等级是以叶绒比作为评价方法,但缺乏科学数据。实验采用扫描电镜、Image Pro Plus、Van Soest法和同步热分析(TGA/DSC)表征(经粉碎机加工)不同叶绒比艾绒的燃烧差异的科学内涵。结果表明自然...艾绒质量是影响灸热疗效的关键因素,传统艾绒等级是以叶绒比作为评价方法,但缺乏科学数据。实验采用扫描电镜、Image Pro Plus、Van Soest法和同步热分析(TGA/DSC)表征(经粉碎机加工)不同叶绒比艾绒的燃烧差异的科学内涵。结果表明自然非腺毛、3∶1、5∶1、10∶1和15∶1不同叶绒比艾绒的非腺毛长度的中位数分别为542.46、303.24、291.18、220.69、170.61μm;随着叶绒比增加,艾绒的纤维素含量显著增加(P<0.05),燃烧参数(T_(i)、t_(i)、D_(i)、C、-R_(p)、-R_(v)、S、D_(b)、J_(总))均增大。相关性结果表明,艾绒的燃烧性能(T_(i)、-R_(v)、t_(i)、J_(2))与纤维素含量呈显著正相关;非腺毛长度1~200μm与J_(2)呈显著正相关;非腺毛长度200~600μm与J_(1)、T_(peak2)、T_(peak1)、-R_(v)呈显著正相关,与J_(总)、T_(b)、t_(b)呈显著负相关。叶绒比增大,艾绒中非腺毛变短、纤维素含量增加,更有利于提高着火性能、释放热量,燃烧更温和持久。“非腺毛-纤维素-TGA/DSC”量化评价方法科学揭示不同叶绒比艾绒的燃烧科学内涵,为不同叶绒比艾绒的质量评价方法建立提供了科学依据。展开更多
基金supported by National Natural Science Foundation of China (81073002)a Basic Research Program of the Ministry of S&T of China (2015FY111500-110)
文摘Objective： To compare the chemical characters of Sparganii Rhizoma from different areas via chromatographic analysis and to establish a sensitive LC/MS method for quality assessment of Sparganii Rhizoma.Methods： Under the optimised HPLC-PDA chromatographic conditions,twenty batches of Sparganii Rhizoma were analyzed by HPLC fingerprints.Principal component analysis（PCA）,orthogonal projections to latent structures discriminant analysis（OPLS-DA）and hierarchical cluster analysis（HCA）were performed based on all peak areas of Sparganii Rhizoma fingerprints.Meanwhile,part of common peaks were subsequently quantified by UFLC-QTRAP-MS.Results： The similarity values of HPLC fingerprints fluctuated in a wide range of 0.511–0.973,which showed variable differences of chemical characters among Sparganii Rhizoma from twenty habitats.PCA,OPLS-DA and HCA indicated that samples could be divided into five groups with different chemical characters,which generally corresponded with their geographical distributions.A total of 31 peaks in HPLC fingerprints were marked,and eight of them were identified and quantified.The quantitative result was generally in agreement with the classifications based on HPLC fingerprints,which indicated that Sparganii Rhizoma samples from eastern China mostly contained more contents including phenolic acids and flavonoids.Conclusion： This study not only proved that there were relationships between geographic distributions and internal chemical compositions of plants,which could provide evidence to the traditional Chinese medicine concept ＂geo-authentic＂,but also supplied a sensitive and rapid simultaneous quantitive method for the quality estimation of Sparganii Rhizoma.
文摘艾绒质量是影响灸热疗效的关键因素,传统艾绒等级是以叶绒比作为评价方法,但缺乏科学数据。实验采用扫描电镜、Image Pro Plus、Van Soest法和同步热分析(TGA/DSC)表征(经粉碎机加工)不同叶绒比艾绒的燃烧差异的科学内涵。结果表明自然非腺毛、3∶1、5∶1、10∶1和15∶1不同叶绒比艾绒的非腺毛长度的中位数分别为542.46、303.24、291.18、220.69、170.61μm;随着叶绒比增加,艾绒的纤维素含量显著增加(P<0.05),燃烧参数(T_(i)、t_(i)、D_(i)、C、-R_(p)、-R_(v)、S、D_(b)、J_(总))均增大。相关性结果表明,艾绒的燃烧性能(T_(i)、-R_(v)、t_(i)、J_(2))与纤维素含量呈显著正相关;非腺毛长度1~200μm与J_(2)呈显著正相关;非腺毛长度200~600μm与J_(1)、T_(peak2)、T_(peak1)、-R_(v)呈显著正相关,与J_(总)、T_(b)、t_(b)呈显著负相关。叶绒比增大,艾绒中非腺毛变短、纤维素含量增加,更有利于提高着火性能、释放热量,燃烧更温和持久。“非腺毛-纤维素-TGA/DSC”量化评价方法科学揭示不同叶绒比艾绒的燃烧科学内涵,为不同叶绒比艾绒的质量评价方法建立提供了科学依据。
