Aminooxyacetate (AOA) is a pyridoxal phosphate antagonist that inhibits various plant enzymes (including transaminases) which require pyridoxal phosphate as a cofactor and it exhibits phytotoxic and herbicidal propert...Aminooxyacetate (AOA) is a pyridoxal phosphate antagonist that inhibits various plant enzymes (including transaminases) which require pyridoxal phosphate as a cofactor and it exhibits phytotoxic and herbicidal properties. We examined AOA and its analog, </span><i><span style="font-family:Verdana;">N</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">t</span></i><span style="font-family:Verdana;">-butoxycarbonyl-AOA (Boc-AOA) for phytotoxicity, interactions with weed pathogens (bioherbicides), and effects on an important pyridoxal requiring enzyme, cysteine synthase (CS, E.C. 4.2.99.8). Studies were performed on two weeds, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;">, hemp sesbania [</span><i><span style="font-family:Verdana;">Sesbania exaltata</span></i><span style="font-family:Verdana;"> (Raf.) Rybd. Ex A.W. Hill] and sicklepod (</span><i><span style="font-family:Verdana;">Senna obtusifolia</span></i><span style="font-family:Verdana;">), and two pathogens, (</span><i><span style="font-family:Verdana;">Colletotrichum truncatum</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Alternaria cassiae</span></i><span style="font-family:Verdana;">), that are bioherbicidal agents against hemp sesbania and sicklepod, respectively. Pathogenicity tests, and assays for extractable, and </span><i><span style="font-family:Verdana;">in vitro </span></i><span style="font-family:Verdana;">CS activities were utilized. Phytotoxicity bioassays indicated that the bulky </span><i><span style="font-family:Verdana;">t</span></i><span style="font-family:Verdana;">-butoxycarbonyl moiety substitution on the AOA molecule did not substantially hinder expression of biological activity of Boc-AOA in these tests. Generally, spray application of the compounds to young dark-grown seedlings caused little growth effects, but root-feeding of the chemicals reduced growth (stem elongation) in both weeds. Hemp sesbania 展开更多
文摘Aminooxyacetate (AOA) is a pyridoxal phosphate antagonist that inhibits various plant enzymes (including transaminases) which require pyridoxal phosphate as a cofactor and it exhibits phytotoxic and herbicidal properties. We examined AOA and its analog, </span><i><span style="font-family:Verdana;">N</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">t</span></i><span style="font-family:Verdana;">-butoxycarbonyl-AOA (Boc-AOA) for phytotoxicity, interactions with weed pathogens (bioherbicides), and effects on an important pyridoxal requiring enzyme, cysteine synthase (CS, E.C. 4.2.99.8). Studies were performed on two weeds, </span><i><span style="font-family:Verdana;">i.e.</span></i><span style="font-family:Verdana;">, hemp sesbania [</span><i><span style="font-family:Verdana;">Sesbania exaltata</span></i><span style="font-family:Verdana;"> (Raf.) Rybd. Ex A.W. Hill] and sicklepod (</span><i><span style="font-family:Verdana;">Senna obtusifolia</span></i><span style="font-family:Verdana;">), and two pathogens, (</span><i><span style="font-family:Verdana;">Colletotrichum truncatum</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Alternaria cassiae</span></i><span style="font-family:Verdana;">), that are bioherbicidal agents against hemp sesbania and sicklepod, respectively. Pathogenicity tests, and assays for extractable, and </span><i><span style="font-family:Verdana;">in vitro </span></i><span style="font-family:Verdana;">CS activities were utilized. Phytotoxicity bioassays indicated that the bulky </span><i><span style="font-family:Verdana;">t</span></i><span style="font-family:Verdana;">-butoxycarbonyl moiety substitution on the AOA molecule did not substantially hinder expression of biological activity of Boc-AOA in these tests. Generally, spray application of the compounds to young dark-grown seedlings caused little growth effects, but root-feeding of the chemicals reduced growth (stem elongation) in both weeds. Hemp sesbania
