引力波是爱因斯坦最重要的预言之一,是检验广义相对论正确性的重要工具.引力波理论及其探测一直以来都是理论物理学家和实验物理学家感兴趣的研究领域.事实上,随着科学技术的发展,人类已经具备了建造极度灵敏的地面探测器以及空间探测...引力波是爱因斯坦最重要的预言之一,是检验广义相对论正确性的重要工具.引力波理论及其探测一直以来都是理论物理学家和实验物理学家感兴趣的研究领域.事实上,随着科学技术的发展,人类已经具备了建造极度灵敏的地面探测器以及空间探测器的能力,直接探测引力波已经成为现实.2015年9月14日,LIGO首次直接探测到引力波,该信号源自一次双黑洞并合事件,自此人类进入引力波常规化探测阶段,终于拉开了引力波天文学时代的序幕.地面引力波探测器最主要的波源是处于旋近和并合阶段的致密双星.如果在探测到这些波源所辐射出的引力波信号的同时,又能观测到波源对应的电磁波信号,那么引力波信号和电磁波信号可以相互补充,形成新的观测模式.然而单个地面引力波探测器很难准确地探测引力波信号,也不能进行波源精确定位,将多个探测器联网组合,这样既能准确探测引力波信号,又能大幅提高引力波波源的定位精度.本文中,首先介绍探测器联网对引力波信号GW150914源的定位情况,然后介绍了两种最常用的估计定位精度的方法,即马尔科夫链蒙特卡罗(Markov Chain Monte Carlo,MCMC)的方法和解析的方法.最后,选用一种解析的方法讨论未来中国引力波探测器与日本及澳大利亚所组成的探测器网络的定位精度,并给出了中国的较优台址.最后,还讨论了中国加入世界引力波探测器网络行列对引力波波源定位的贡献.展开更多
Atherosclerosis(AS) is a chronic inflammatory disease associated with high morbidity and mortality. The incidence of AS is increasing in the last decades. So development of safe and effective therapeutics for treating...Atherosclerosis(AS) is a chronic inflammatory disease associated with high morbidity and mortality. The incidence of AS is increasing in the last decades. So development of safe and effective therapeutics for treating AS has become prominently important. Although there are numerous chemical drugs available for treating AS, some drugs are not effective and some have serious side effects. Traditional Chinese medicine(TCM) has a long history for the prevention and treatment of AS due to its less side effects and superior efficacy. This paper describes the effectiveness and underlying mechanisms for prevention and treatment of AS by TCM or its active components. Some TCM, e.g. XuemaiNing, Tongxinluo and Salvia miltiorrhiza have been reported to have cardio-protective effect. Some active components of TCM, e.g. saikosaponin-A, kuwanon G, luteolin and β-elemene have been isolated from various TCM and demonstrated to have beneficial effects on prevention and treatment of AS.展开更多
Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery o...Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery of additional triterpenoid saponins, phytoecdysones,polysaccharides, and many other compounds, as well as investigations into their pharmacology. A large number of pharmacological studies have shown RAB and its active components possess a range of pharmacological activities, including anti-tumor, anti-fertility, anti-senile, and anti?inflammatory effects. This review is an up?to?date summary and synthesis of the uses of RAB from phytochemical and pharmacological perspectives.展开更多
A novel schinortriterpenoid(SNT),schinensilactone A(1),characterized by a unique 7,8-seco-1,8-cyclo-schisanartane scaffold,was isolated from the leaves of Schisandra chinensis(Turcz.)Baill,together with a new SNT(schi...A novel schinortriterpenoid(SNT),schinensilactone A(1),characterized by a unique 7,8-seco-1,8-cyclo-schisanartane scaffold,was isolated from the leaves of Schisandra chinensis(Turcz.)Baill,together with a new SNT(schinensilactone B,2)and two known(3,4).Their structures were elucidated using spectroscopy and X-ray diffraction.Furthermore,a hypothetical biosynthetic pathway for 1 was postulated due to its novel carbon skeleton.In addition,1 exhibited significant anti-proliferative activity against Caco-2 cells originat-ing from five different tumor cell lines,and its preliminary mechanism of action was investigated with respect to the expression of apoptosis-related proteins,including P53,Caspase 3,Bax,PUMA,and Bcl2.These biological activities would further our understanding of the food function of Schisandra chinensis leaves.展开更多
A rapid and high selective ultra-performance liquid chromatography(UPLC)with tandem mass spectrometry method for simultaneous determination of six compounds including albiflorin,paeoniflorin,picroside I,picroside II,s...A rapid and high selective ultra-performance liquid chromatography(UPLC)with tandem mass spectrometry method for simultaneous determination of six compounds including albiflorin,paeoniflorin,picroside I,picroside II,saikosaponin A,and saikosaponin D in rat plasma was developed and validated using butyl p-hydroxybenzoate as an internal standard.One-step direct protein precipitation with acetonitrile was used to extract the compounds from the rat plasma samples.Chromatographic separation was achieved using an ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7μm)at a flow rate of 0.4 m L/min,using gradient mode containing 0.1%formic acid in water and acetonitrile were used as the Mobile phase A and B.Electrospray ionization in negative ion mode and multiple reaction monitoring were used to identify and quantify active components.Calibration curves showed good linearity(R^2>0.9908)over a wide concentration range for all compounds.The intra-and interday precision(relative standard deviation)ranged 2.4%–7.0%and 2.6%–8.0%,respectively.The accuracy(relative error)was from-13.0%to 13.2%at all quality control levels.The recovery ranged from 81.1%to 92.5%.The validated method was successfully applied to pharmacokinetic study in rats after oral administration of Qing Gan-Shu Yu-Fang.The results show that one can draw a conclusion that these six active ingredients can be quickly absorbed and play a pharmacodynamic role rapidly in vivo.展开更多
Given the sequence of Chs gene promoter from Arabidopsis thaliana reported in GenBank （AF248988）, a pair of specific PCR primers was designed with the Primer Premier 5.0 software. PCR products of about 0.5 kb were s...Given the sequence of Chs gene promoter from Arabidopsis thaliana reported in GenBank （AF248988）, a pair of specific PCR primers was designed with the Primer Premier 5.0 software. PCR products of about 0.5 kb were successfully amplified with the genome DNA of A. thaliana as a DNA template and Taq polymerase as DNA polymerase. The purified PCR products were ligated to the pMD18-T vector. The sequencing result showed that the Chs promoter from A. thaliana was 531 bp long. Sequence alignment analysis based on the DNAMAN software revealed that the sequence similarity between the cloned promoter and target promoter （AF248988） was up to 100%. Online PLACE analysis indicated that the Chs promoter contained cis-elements such as TATA-box, CAAT-box, pollen-box, G-box, ACGT-containing element, R response element, Myb recognition element and TACPyAT-box. At the same time, a plant expression vectorpAtChs：：GUS which fused the Chs promoter and the marker gene GUS was successfully constructed.展开更多
In recent years,drug development and research have gradually shifted from chemical synthesis to biopharmaceutical and natural drugs.Natural medicines,such as traditional Chinese medicine,have been among the first stud...In recent years,drug development and research have gradually shifted from chemical synthesis to biopharmaceutical and natural drugs.Natural medicines,such as traditional Chinese medicine,have been among the first studied because of their long medicinal history,simplicity,and the relatively low cost of research.Among them,Xanthii Fructus(XF)is famous for the treatment of sinusitis.In this article,the achievements of research on XF from 1953 to 2020 are systematically reviewed,focusing on the aspects of chemical constituents,pharmacological effects,clinical applications,toxicity and side effects,and processing methods.To date,there have been significant advances in both the phytochemistry and pharmacology of XF.Some traditional uses have been validated and clarified in modern pharmacological studies.However,its mechanism of action in the treatment of allergic diseases has not been satisfactorily explained.Further in vitro and in vivo studies are required to rationally develop new drugs and to elucidate the therapeutic potential of XF.A comprehensive evaluation of XF and an understanding of network pharmacology are also needed.展开更多
The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid （ACC） to ethylene by ACC oxidase （ACO）. In our investigation, a cross-introns genomic DNA gene encoding A...The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid （ACC） to ethylene by ACC oxidase （ACO）. In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO （FJ855434） has 1281 bases, containing four exons and three introns, en- coding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.展开更多
The long-term clinical practice of traditional Chinese medicine(TCM)confirms its importance and essential role in the health care system in China,especially in the prevention and treatment of chronic diseases.TCM is t...The long-term clinical practice of traditional Chinese medicine(TCM)confirms its importance and essential role in the health care system in China,especially in the prevention and treatment of chronic diseases.TCM is the holistic medicine under the guidance of system theory,emphasizing harmony between human and nature,focusing on equilibrium and balance,and focusing on展开更多
The wave functions of the n1,3p (n=2, 3, 4) and the n 1,3D (n=3, 4, 5 ) low-lying states of the helium atom are expanded into the complete sets of the symmetrically adapted basis functions from hyperspherical harmonic...The wave functions of the n1,3p (n=2, 3, 4) and the n 1,3D (n=3, 4, 5 ) low-lying states of the helium atom are expanded into the complete sets of the symmetrically adapted basis functions from hyperspherical harmonic functions in the angle part and of generalized Laguerre functions in the radial part respectively, and are then augmented by the simplest type of Jastrow correlation factor to incorporate electron-nucleus cusp only. The excellent agreement between the present nonrelativistic eigen-energies and those from the sophisticated configuration interaction (CI) method for the examined states indicates that the hyperspherical harmonic method can also be applied to the P and the D excited states of the helium atom.展开更多
文摘引力波是爱因斯坦最重要的预言之一,是检验广义相对论正确性的重要工具.引力波理论及其探测一直以来都是理论物理学家和实验物理学家感兴趣的研究领域.事实上,随着科学技术的发展,人类已经具备了建造极度灵敏的地面探测器以及空间探测器的能力,直接探测引力波已经成为现实.2015年9月14日,LIGO首次直接探测到引力波,该信号源自一次双黑洞并合事件,自此人类进入引力波常规化探测阶段,终于拉开了引力波天文学时代的序幕.地面引力波探测器最主要的波源是处于旋近和并合阶段的致密双星.如果在探测到这些波源所辐射出的引力波信号的同时,又能观测到波源对应的电磁波信号,那么引力波信号和电磁波信号可以相互补充,形成新的观测模式.然而单个地面引力波探测器很难准确地探测引力波信号,也不能进行波源精确定位,将多个探测器联网组合,这样既能准确探测引力波信号,又能大幅提高引力波波源的定位精度.本文中,首先介绍探测器联网对引力波信号GW150914源的定位情况,然后介绍了两种最常用的估计定位精度的方法,即马尔科夫链蒙特卡罗(Markov Chain Monte Carlo,MCMC)的方法和解析的方法.最后,选用一种解析的方法讨论未来中国引力波探测器与日本及澳大利亚所组成的探测器网络的定位精度,并给出了中国的较优台址.最后,还讨论了中国加入世界引力波探测器网络行列对引力波波源定位的贡献.
基金supported by the Excellent Innovative Talent Support Program of Heilongjiang University of Chinese Medicine(No.2012RCD05)
文摘Atherosclerosis(AS) is a chronic inflammatory disease associated with high morbidity and mortality. The incidence of AS is increasing in the last decades. So development of safe and effective therapeutics for treating AS has become prominently important. Although there are numerous chemical drugs available for treating AS, some drugs are not effective and some have serious side effects. Traditional Chinese medicine(TCM) has a long history for the prevention and treatment of AS due to its less side effects and superior efficacy. This paper describes the effectiveness and underlying mechanisms for prevention and treatment of AS by TCM or its active components. Some TCM, e.g. XuemaiNing, Tongxinluo and Salvia miltiorrhiza have been reported to have cardio-protective effect. Some active components of TCM, e.g. saikosaponin-A, kuwanon G, luteolin and β-elemene have been isolated from various TCM and demonstrated to have beneficial effects on prevention and treatment of AS.
基金supported by the National Natural Science Foundation of China (grant number 81803690)the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (grant number 2012RCQ20)+5 种基金the Heilongjiang Postdoctoral Innovation Foundation (grant number LBH-Z10020)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (grant number 2013bs04)the Natural Science Foundation of Heilongjiang Province (grant number H201324)the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant number 20132327120003)the Heilongjiang Postdoctoral Scientific Research Developmental Fund (grant number LBH-Q16210)the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (grant number UNPYSCT-2017219)
文摘Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery of additional triterpenoid saponins, phytoecdysones,polysaccharides, and many other compounds, as well as investigations into their pharmacology. A large number of pharmacological studies have shown RAB and its active components possess a range of pharmacological activities, including anti-tumor, anti-fertility, anti-senile, and anti?inflammatory effects. This review is an up?to?date summary and synthesis of the uses of RAB from phytochemical and pharmacological perspectives.
基金This work was financially supported by the_Heilongjiang Touyan Innovation Team Program and the National Natural Science Foundation of China(NSFC)(81473325).
文摘A novel schinortriterpenoid(SNT),schinensilactone A(1),characterized by a unique 7,8-seco-1,8-cyclo-schisanartane scaffold,was isolated from the leaves of Schisandra chinensis(Turcz.)Baill,together with a new SNT(schinensilactone B,2)and two known(3,4).Their structures were elucidated using spectroscopy and X-ray diffraction.Furthermore,a hypothetical biosynthetic pathway for 1 was postulated due to its novel carbon skeleton.In addition,1 exhibited significant anti-proliferative activity against Caco-2 cells originat-ing from five different tumor cell lines,and its preliminary mechanism of action was investigated with respect to the expression of apoptosis-related proteins,including P53,Caspase 3,Bax,PUMA,and Bcl2.These biological activities would further our understanding of the food function of Schisandra chinensis leaves.
基金supported financially by the National Natural Science Foundation of China (Grant No. 81973604, 81803690and 81703684)the Innovative Talents Funding of Heilongjiang University of Chinese Medicine(Grant No.2018RCD25)+8 种基金the National natural science foundation matching project (Grant No. 2018PT02)the National natural Science Foundation Matching Project (Grant No. 2017PT01)the Graduate Innovative Research Project Foundation of Heilongjiang University of Chinese Medicine (Grant No. 2019yjscx013)the Postdoctoral Initial Fund of Heilongjiang Provincethe University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (Grant No. UNPYSCT2017215 and UNPYSCT2017219)the Natural Science Foundation of Heilongjiang Province (Grant No. H2015037)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (Grant No. 2014bs05)the Application Technology Research and Development Projects of Harbin Technology Bureau (Grant No. 2014RFQXJ149)Heilongjiang Postdoctoral Scientific Research Developmental Fund (Grant No.LBHQ16210 and LBH-Q17161)
文摘A rapid and high selective ultra-performance liquid chromatography(UPLC)with tandem mass spectrometry method for simultaneous determination of six compounds including albiflorin,paeoniflorin,picroside I,picroside II,saikosaponin A,and saikosaponin D in rat plasma was developed and validated using butyl p-hydroxybenzoate as an internal standard.One-step direct protein precipitation with acetonitrile was used to extract the compounds from the rat plasma samples.Chromatographic separation was achieved using an ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7μm)at a flow rate of 0.4 m L/min,using gradient mode containing 0.1%formic acid in water and acetonitrile were used as the Mobile phase A and B.Electrospray ionization in negative ion mode and multiple reaction monitoring were used to identify and quantify active components.Calibration curves showed good linearity(R^2>0.9908)over a wide concentration range for all compounds.The intra-and interday precision(relative standard deviation)ranged 2.4%–7.0%and 2.6%–8.0%,respectively.The accuracy(relative error)was from-13.0%to 13.2%at all quality control levels.The recovery ranged from 81.1%to 92.5%.The validated method was successfully applied to pharmacokinetic study in rats after oral administration of Qing Gan-Shu Yu-Fang.The results show that one can draw a conclusion that these six active ingredients can be quickly absorbed and play a pharmacodynamic role rapidly in vivo.
基金supported by the National Natural Science Foundation of China (Grant No.30740013)the Key Laboratory for Genetics and Breeding in Forestry Trees and Ornamental Plants,Ministry of Education (03-05)
文摘Given the sequence of Chs gene promoter from Arabidopsis thaliana reported in GenBank （AF248988）, a pair of specific PCR primers was designed with the Primer Premier 5.0 software. PCR products of about 0.5 kb were successfully amplified with the genome DNA of A. thaliana as a DNA template and Taq polymerase as DNA polymerase. The purified PCR products were ligated to the pMD18-T vector. The sequencing result showed that the Chs promoter from A. thaliana was 531 bp long. Sequence alignment analysis based on the DNAMAN software revealed that the sequence similarity between the cloned promoter and target promoter （AF248988） was up to 100%. Online PLACE analysis indicated that the Chs promoter contained cis-elements such as TATA-box, CAAT-box, pollen-box, G-box, ACGT-containing element, R response element, Myb recognition element and TACPyAT-box. At the same time, a plant expression vectorpAtChs：：GUS which fused the Chs promoter and the marker gene GUS was successfully constructed.
基金financially supported by the National Natural Science Foundation of China(No.81703684,81803690,and 81973604)the Graduate Innovative Research Project Foundation of Heilongjiang University of Chinese Medicine(No.2019yjsc×013)+8 种基金the Innovative Talents Funding of Heilongjiang University of Chinese Medicine(No.2018RCD25)the National Natural Science Foundation Matching Project(No.2018PT02)the Postdoctoral Initial Fund of Heilongjiang Provincethe University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province(No.UNPYSCT 2017219 and UNPYSCT 2017215)the National Natural Science Foundation Matching Project(No.2017PT01)Heilongjiang Postdoctoral Scientific Research Developmental Fund(No.LBH Q16210 and LBH-Q17161)the Natural Science Foundation of Heilongjiang Province(No.H2015037)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation(No.2014bs05)the Application Technology Research and Development Projects of Harbin Technology Bureau(No.2014RFQXJ149)
文摘In recent years,drug development and research have gradually shifted from chemical synthesis to biopharmaceutical and natural drugs.Natural medicines,such as traditional Chinese medicine,have been among the first studied because of their long medicinal history,simplicity,and the relatively low cost of research.Among them,Xanthii Fructus(XF)is famous for the treatment of sinusitis.In this article,the achievements of research on XF from 1953 to 2020 are systematically reviewed,focusing on the aspects of chemical constituents,pharmacological effects,clinical applications,toxicity and side effects,and processing methods.To date,there have been significant advances in both the phytochemistry and pharmacology of XF.Some traditional uses have been validated and clarified in modern pharmacological studies.However,its mechanism of action in the treatment of allergic diseases has not been satisfactorily explained.Further in vitro and in vivo studies are required to rationally develop new drugs and to elucidate the therapeutic potential of XF.A comprehensive evaluation of XF and an understanding of network pharmacology are also needed.
基金supported by the National Natural Science Foundation of China (No. 30740013)the Foundation for Young University Key Teachers of the Educational Commission of Henan Province, China(No. 2010GGJS-075)
文摘The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid （ACC） to ethylene by ACC oxidase （ACO）. In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO （FJ855434） has 1281 bases, containing four exons and three introns, en- coding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.
文摘The long-term clinical practice of traditional Chinese medicine(TCM)confirms its importance and essential role in the health care system in China,especially in the prevention and treatment of chronic diseases.TCM is the holistic medicine under the guidance of system theory,emphasizing harmony between human and nature,focusing on equilibrium and balance,and focusing on
基金Supported by the National Natural Science Foundation of China(No. 29703003).
文摘The wave functions of the n1,3p (n=2, 3, 4) and the n 1,3D (n=3, 4, 5 ) low-lying states of the helium atom are expanded into the complete sets of the symmetrically adapted basis functions from hyperspherical harmonic functions in the angle part and of generalized Laguerre functions in the radial part respectively, and are then augmented by the simplest type of Jastrow correlation factor to incorporate electron-nucleus cusp only. The excellent agreement between the present nonrelativistic eigen-energies and those from the sophisticated configuration interaction (CI) method for the examined states indicates that the hyperspherical harmonic method can also be applied to the P and the D excited states of the helium atom.
