BACKGROUND：As the regulators of cytokines, suppressors of cytokine signaling （SOCS） play an important role in the inflammation reaction. Some studies found that SOCS-1 and SOCS-3 were involved in the pathogenesis o...BACKGROUND：As the regulators of cytokines, suppressors of cytokine signaling （SOCS） play an important role in the inflammation reaction. Some studies found that SOCS-1 and SOCS-3 were involved in the pathogenesis of some inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease. But the expressions of SOCS in coronary heart disease have not yet been reported. This study aimed to investigate the expression and clinical significance of SOCS-1 and SOCS-3 in the myocardium of patients with sudden cardiac death （SCD）.METHODS： Myocardial autopsy specimens were collected from 24 patients at the Forensic Medicine Department of Sun Yat-Sen University, Guangzhou, China between 2005 and 2006. Of them, 9 patients had autopsy findings consistent with coronary atherosclerosis （non-myocardial infarction） leading to SCD （non-MI group）, 7 died of acute myocardial infaction （MI group）, and 8 died from traffic accidents and trauma （control group）. The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the myocardium of the non-MI, MI and control groups were detected using RT-PCR. The levels of SOCS-1 and SOCS-3 proteins were detected using immunohistochemistry. Statistical analyses were performed using SPSS version 13.0 sottware and the data were analyzed by ANOVA.RESULTS： The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the non-MI and MI groups were significantly higher than those in the control group[（0.788±0.101）, （0.741±0.111） vs. （0.436±0.044）, （P〈0.01）; （0.841±0.092）, （0.776±0.070） vs. （0.454±0.076）, （P〈0.01）] respectively. The antibody-positive cells of SOCS-1 protein in the myocardium of the non-MI and MI groups were significantly higher than those in the myocardium of the control group[（320.00±48.48）, （347.14±70.88） vs. （42.50±10.35）, （P〈0.01）] respectively. The antibody-positive cells of SOCS-3 protein in the myocardium of the non-MI and MI groups were significantly higher than those in the myocardium of the contr展开更多
目的了解我国静脉吸毒人群中（injecting drug user,IDU）艾滋病病毒感染者和病人（HIV/AIDS）抗病毒治疗后病毒抑制失败及耐药状况。方法利用我国耐药监测数据库取得接受抗病毒治疗的静脉吸毒HIV/AIDS的基本人口学特征、抗病毒治疗情...目的了解我国静脉吸毒人群中（injecting drug user,IDU）艾滋病病毒感染者和病人（HIV/AIDS）抗病毒治疗后病毒抑制失败及耐药状况。方法利用我国耐药监测数据库取得接受抗病毒治疗的静脉吸毒HIV/AIDS的基本人口学特征、抗病毒治疗情况及病毒学数据,分析该人群治疗后病毒抑制失败和耐药发生原因。结果本研究中初始抗病毒治疗的静脉吸毒HIV/AIDS 1 395人,平均抗病毒治疗时间为23.9个月,病毒抑制失败率（病毒载量≥1 000拷贝/ml）为21.4%（298/1 395）,广西和云南地区病毒抑制失败率12.2%（60/493）,其他省份的病毒抑制失败率26.4%（238/902）,总体耐药率为8.4%（117/1 395）。多因素logistic回归结果显示少数民族（AOR=2.1,95%CI：1.6～2.9）、最近治疗方案含LPV/r（克力芝）（AOR=3.6,95%CI：2.4～5.4）、近一个月漏服（AOR=2.0,95%CI：1.4～3.0）、开始治疗时间为2011-2013年（AOR=2.2,95%CI：1.5～3.2）的HIV/AIDS病毒抑制失败率高;年龄〉35岁（AOR=0.7,95%CI：0.6～1.0）、广西和云南（AOR=0.3,95%CI：0.2～0.5）的静脉吸毒HIV/AIDS在接受抗病毒治疗后病毒抑制失败率低。结论我国静脉吸毒HIV/AIDS抗病毒治疗后病毒抑制失败率较高,但广西和云南地区治疗者的病毒抑制失败率低于其他地区。服药依从性是主要影响因素之一。展开更多
A two-step alkaline-acidic leaching process was conducted to separate the cryolite from spent pot lining and to purify the carbon. The influencing factors of temperature, time, and the ratio of liquid to solid in alka...A two-step alkaline-acidic leaching process was conducted to separate the cryolite from spent pot lining and to purify the carbon. The influencing factors of temperature, time, and the ratio of liquid to solid in alkaline and acidic leaching were investigated. The results show that the recovery of soluble compounds of Na3AlF6 and Al2O3 dissolving into the solution during the NaOH leaching is 65.0%,and the purity of carbon reaches 72.7%. During the next step of HCl leaching, the recovery of soluble compounds of CaF2 and NaAl11O17 dissolving into the HCl solution is 96.2%, and the carbon purity increases to 96.4%. By mixing the acidic leaching solution and the alkaline leaching solution, the cryolite precipitates under a suitable conditions of pH value 9 at 70 °C for 2 h. The cryolite precipitating rate is 95.6%, and the purity of Na3AlF6 obtained is 96.4%.展开更多
文摘BACKGROUND：As the regulators of cytokines, suppressors of cytokine signaling （SOCS） play an important role in the inflammation reaction. Some studies found that SOCS-1 and SOCS-3 were involved in the pathogenesis of some inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease. But the expressions of SOCS in coronary heart disease have not yet been reported. This study aimed to investigate the expression and clinical significance of SOCS-1 and SOCS-3 in the myocardium of patients with sudden cardiac death （SCD）.METHODS： Myocardial autopsy specimens were collected from 24 patients at the Forensic Medicine Department of Sun Yat-Sen University, Guangzhou, China between 2005 and 2006. Of them, 9 patients had autopsy findings consistent with coronary atherosclerosis （non-myocardial infarction） leading to SCD （non-MI group）, 7 died of acute myocardial infaction （MI group）, and 8 died from traffic accidents and trauma （control group）. The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the myocardium of the non-MI, MI and control groups were detected using RT-PCR. The levels of SOCS-1 and SOCS-3 proteins were detected using immunohistochemistry. Statistical analyses were performed using SPSS version 13.0 sottware and the data were analyzed by ANOVA.RESULTS： The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the non-MI and MI groups were significantly higher than those in the control group[（0.788±0.101）, （0.741±0.111） vs. （0.436±0.044）, （P〈0.01）; （0.841±0.092）, （0.776±0.070） vs. （0.454±0.076）, （P〈0.01）] respectively. The antibody-positive cells of SOCS-1 protein in the myocardium of the non-MI and MI groups were significantly higher than those in the myocardium of the control group[（320.00±48.48）, （347.14±70.88） vs. （42.50±10.35）, （P〈0.01）] respectively. The antibody-positive cells of SOCS-3 protein in the myocardium of the non-MI and MI groups were significantly higher than those in the myocardium of the contr
基金Project (50804010) supported by the National Natural Science Foundation of China
文摘A two-step alkaline-acidic leaching process was conducted to separate the cryolite from spent pot lining and to purify the carbon. The influencing factors of temperature, time, and the ratio of liquid to solid in alkaline and acidic leaching were investigated. The results show that the recovery of soluble compounds of Na3AlF6 and Al2O3 dissolving into the solution during the NaOH leaching is 65.0%,and the purity of carbon reaches 72.7%. During the next step of HCl leaching, the recovery of soluble compounds of CaF2 and NaAl11O17 dissolving into the HCl solution is 96.2%, and the carbon purity increases to 96.4%. By mixing the acidic leaching solution and the alkaline leaching solution, the cryolite precipitates under a suitable conditions of pH value 9 at 70 °C for 2 h. The cryolite precipitating rate is 95.6%, and the purity of Na3AlF6 obtained is 96.4%.
