To adapt to changing environments, plants have evolved elaborate regulatory mechanisms balancing their growth with stress responses. It is currently unclear whether and how the tryptophan (Trp), the growth-related hor...To adapt to changing environments, plants have evolved elaborate regulatory mechanisms balancing their growth with stress responses. It is currently unclear whether and how the tryptophan (Trp), the growth-related hormone auxin, and the stress hormone abscisic acid (ABA) are coordinated in this trade-off. Here, we show that tryptophan synthase β subunit 1 (TSB1) is involved in the coordination of Trp and ABA, thereby affecting plant growth and abiotic stress responses. Plants experiencing high salinity or drought display reduced TSB1 expression, resulting in decreased Trp and auxin accumulation and thus reduced growth. In comparison with the wild type, amiR-TSB1 lines and TSB1 mutants exhibited repressed growth under non-stress conditions but had enhanced ABA accumulation and stress tolerance when subjected to salt or drought stress. Furthermore, we found that TSB1 interacts with and inhibits β-glucosidase 1 (BG1), which hydrolyses glucose-conjugated ABA into active ABA. Mutation of BG1 in the amiR-TSB1 lines compromised their increased ABA accumulation and enhanced stress tolerance. Moreover, stress-induced H2O2 disrupted the interaction between TSB1 and BG1 by sulfenylating cysteine-308 of TSB1, relieving the TSB1-mediated inhibition of BG1 activity. Taken together, we revealed that TSB1 serves as a key coordinator of plant growth and stress responses by balancing Trp and ABA homeostasis.展开更多
Climate change is resulting in more frequent and rapidly changing temperatures at both extremes that severely affect the growth and production of plants,particularly crops.Oxidative stress caused by high temperatures ...Climate change is resulting in more frequent and rapidly changing temperatures at both extremes that severely affect the growth and production of plants,particularly crops.Oxidative stress caused by high temperatures is one of the most damaging factors for plants.However,the role of hydrogen peroxide(H_(2)O_(2))in modulating plant thermotolerance is largely unknown,and the regulation of photorespiration essential for C3 species remains to be fully clarified.Here,we report that heat stress promotes H2O2 accumulation in chloroplasts and that H2O2 stimulates sulfenylation of the chloroplast-localized photorespiratory enzyme 2-phosphoglycolate phosphatase 1(PGLP1)at cysteine 86,inhibiting its activity and promoting the accumulation of the toxic metabolite 2-phosphoglycolate.We also demonstrate that PGLP1 has a positive function in plant thermotolerance,as PGLP1 antisense lines have greater heat sensitivity and PGLP1-overexpressing plants have higher heat-stress tolerance than the wild type.Together,our results demonstrate that heat-induced H2O2 in chloroplasts sulfenylates and inhibits PGLP1 to modulate plant thermotolerance.Furthermore,targeting CATALASE2 to chloroplasts can largely prevent the heatinduced overaccumulation of H2O2 and the sulfenylation of PGLP1,thus conferring thermotolerance without a plant growth penalty.These findings reveal that heat-induced H2O2 in chloroplasts is important for heat-caused plant damage.展开更多
Traditional laparoscopic radical prostatectomy is a treatment choice in many developing countries and regions for most patients with localized prostate cancer; however, no system for predicting surgical difficulty and...Traditional laparoscopic radical prostatectomy is a treatment choice in many developing countries and regions for most patients with localized prostate cancer; however, no system for predicting surgical difficulty and risk has been established. This study aimed to propose a simple and standard preoperative classification system of prostate cancer using preoperative data to predict surgical difficulty and risk and to evaluate the relationship between the data and postoperative complications. We collected data from 236 patients and divided them into three groups to evaluate and validate the relationships among preoperative, operative, and postoperative data. This new scoring system is based on the body mass index, ultrasonic prostate volume, preoperative prostate-specific antigen level, middle lobe protrusion, and clinical stage. In the scoring group, we classified 89 patients into two groups： the low-risk group （score of 〈4） and high-risk group （score of ~4）, and then compared the postoperative data between the two groups. The positive surgical margin rate was higher in the high-risk group than low-risk group. The results in validation Groups A and B were similar to those in the scoring group. The focus of our scoring system is to allow for preliminary assessment of surgical difficulty by collecting the patients＇ basic information. Urologists can easily use the scoring system to evaluate the surgical difficulty and predict the risks of a positive surgical margin and urinary incontinence in patients undergoing laparoscopic radical prostatectomy.展开更多
By incorporating the strain gradient elasticity into the classical Bernoulli-Euler beam and Timoshenko beam models, the size-dependent characteristics of wave propaga- tion in micro/nanobeams is studied. The formulati...By incorporating the strain gradient elasticity into the classical Bernoulli-Euler beam and Timoshenko beam models, the size-dependent characteristics of wave propaga- tion in micro/nanobeams is studied. The formulations of dis- persion relation are explicitly derived for both strain gradi- ent beam models, and presented for different material length scale parameters （MLSPs）. For both phenomenological size- dependent beam models, the angular frequency, phase veloc- ity and group velocity increase with increasing wave num- ber. However, the velocity ratios approach different values for different beam models, indicating an interesting behavior of the asymptotic velocity ratio. The present theory is also compared with the nonlocal continuum beam models.展开更多
To the Editor:Leukemia is a malignant proliferative disease that has become the most common malignancy in children,accounting for 40.5%of malignant cancers children.[1]Although chemotherapy can effectively treat child...To the Editor:Leukemia is a malignant proliferative disease that has become the most common malignancy in children,accounting for 40.5%of malignant cancers children.[1]Although chemotherapy can effectively treat children,some patients with high-risk and relapsed acute lymphoblastic leukemia(ALL)and acute myelocytic leukemia(AML)failed to achieve long-term relief.For these patients,allogeneic hematopoietic stem cell transplantation(allo-HSCT)is the best choice.However,many factors may impact the efficacy of allo-HSCT.We retrospectively analyzed the clinical data of 71 children with leukemia treated using allo-HSCT to observe the clinical efficacy and analyze the possible influencing factors.展开更多
We demonstrate that the Mg-doping in barriers can partially screen the polarization fields of InGaN-based green light-emitting diodes.The photocurrent spectra show that the Mg-doping samples have smaller polarization ...We demonstrate that the Mg-doping in barriers can partially screen the polarization fields of InGaN-based green light-emitting diodes.The photocurrent spectra show that the Mg-doping samples have smaller polarization fields and the blue shift of the peak with increasing current is observed.The reduction of polarization fields can be attributed to the screening of the impurity holes generated by the Mg atoms in the barriers.The efficiency droop is sensitive to the Mg-doping concentration in barriers,while the sample with Mg concentration of 5×10^(19) cm^(-3) exhibits the lowest efficiency degradation of 12.4%at a high injection current.展开更多
基金supported by National Natural Science Foundation of China(#no.31830007,#no.32000150.and#no.31670251)the Natural Science Foundation of Henan Province(#no.222300420401)。
文摘To adapt to changing environments, plants have evolved elaborate regulatory mechanisms balancing their growth with stress responses. It is currently unclear whether and how the tryptophan (Trp), the growth-related hormone auxin, and the stress hormone abscisic acid (ABA) are coordinated in this trade-off. Here, we show that tryptophan synthase β subunit 1 (TSB1) is involved in the coordination of Trp and ABA, thereby affecting plant growth and abiotic stress responses. Plants experiencing high salinity or drought display reduced TSB1 expression, resulting in decreased Trp and auxin accumulation and thus reduced growth. In comparison with the wild type, amiR-TSB1 lines and TSB1 mutants exhibited repressed growth under non-stress conditions but had enhanced ABA accumulation and stress tolerance when subjected to salt or drought stress. Furthermore, we found that TSB1 interacts with and inhibits β-glucosidase 1 (BG1), which hydrolyses glucose-conjugated ABA into active ABA. Mutation of BG1 in the amiR-TSB1 lines compromised their increased ABA accumulation and enhanced stress tolerance. Moreover, stress-induced H2O2 disrupted the interaction between TSB1 and BG1 by sulfenylating cysteine-308 of TSB1, relieving the TSB1-mediated inhibition of BG1 activity. Taken together, we revealed that TSB1 serves as a key coordinator of plant growth and stress responses by balancing Trp and ABA homeostasis.
基金supported by grants from the National Key Research and Development Program of China(2022YFD1200400)the National Natural Science Foundation of China(32301864 and 32322010).
文摘Climate change is resulting in more frequent and rapidly changing temperatures at both extremes that severely affect the growth and production of plants,particularly crops.Oxidative stress caused by high temperatures is one of the most damaging factors for plants.However,the role of hydrogen peroxide(H_(2)O_(2))in modulating plant thermotolerance is largely unknown,and the regulation of photorespiration essential for C3 species remains to be fully clarified.Here,we report that heat stress promotes H2O2 accumulation in chloroplasts and that H2O2 stimulates sulfenylation of the chloroplast-localized photorespiratory enzyme 2-phosphoglycolate phosphatase 1(PGLP1)at cysteine 86,inhibiting its activity and promoting the accumulation of the toxic metabolite 2-phosphoglycolate.We also demonstrate that PGLP1 has a positive function in plant thermotolerance,as PGLP1 antisense lines have greater heat sensitivity and PGLP1-overexpressing plants have higher heat-stress tolerance than the wild type.Together,our results demonstrate that heat-induced H2O2 in chloroplasts sulfenylates and inhibits PGLP1 to modulate plant thermotolerance.Furthermore,targeting CATALASE2 to chloroplasts can largely prevent the heatinduced overaccumulation of H2O2 and the sulfenylation of PGLP1,thus conferring thermotolerance without a plant growth penalty.These findings reveal that heat-induced H2O2 in chloroplasts is important for heat-caused plant damage.
文摘Traditional laparoscopic radical prostatectomy is a treatment choice in many developing countries and regions for most patients with localized prostate cancer; however, no system for predicting surgical difficulty and risk has been established. This study aimed to propose a simple and standard preoperative classification system of prostate cancer using preoperative data to predict surgical difficulty and risk and to evaluate the relationship between the data and postoperative complications. We collected data from 236 patients and divided them into three groups to evaluate and validate the relationships among preoperative, operative, and postoperative data. This new scoring system is based on the body mass index, ultrasonic prostate volume, preoperative prostate-specific antigen level, middle lobe protrusion, and clinical stage. In the scoring group, we classified 89 patients into two groups： the low-risk group （score of 〈4） and high-risk group （score of ~4）, and then compared the postoperative data between the two groups. The positive surgical margin rate was higher in the high-risk group than low-risk group. The results in validation Groups A and B were similar to those in the scoring group. The focus of our scoring system is to allow for preliminary assessment of surgical difficulty by collecting the patients＇ basic information. Urologists can easily use the scoring system to evaluate the surgical difficulty and predict the risks of a positive surgical margin and urinary incontinence in patients undergoing laparoscopic radical prostatectomy.
基金supported by the National Natural Science Foundation of China(11202117,11272186,11172231 and 50928601)the Postdoctoral Science Foundation of China(2012M521326)+3 种基金the Natural Science Fund of Shandong Province(ZR2012AM014 and BS2012ZZ006)Independent Innovation Fund of Shandong University(2011GN055)National Science Foundation(CMMI-0643726),DARPA(W91CRB-11-C-0112)Changjiang Scholar Program from Ministry of Education of China
文摘By incorporating the strain gradient elasticity into the classical Bernoulli-Euler beam and Timoshenko beam models, the size-dependent characteristics of wave propaga- tion in micro/nanobeams is studied. The formulations of dis- persion relation are explicitly derived for both strain gradi- ent beam models, and presented for different material length scale parameters （MLSPs）. For both phenomenological size- dependent beam models, the angular frequency, phase veloc- ity and group velocity increase with increasing wave num- ber. However, the velocity ratios approach different values for different beam models, indicating an interesting behavior of the asymptotic velocity ratio. The present theory is also compared with the nonlocal continuum beam models.
文摘To the Editor:Leukemia is a malignant proliferative disease that has become the most common malignancy in children,accounting for 40.5%of malignant cancers children.[1]Although chemotherapy can effectively treat children,some patients with high-risk and relapsed acute lymphoblastic leukemia(ALL)and acute myelocytic leukemia(AML)failed to achieve long-term relief.For these patients,allogeneic hematopoietic stem cell transplantation(allo-HSCT)is the best choice.However,many factors may impact the efficacy of allo-HSCT.We retrospectively analyzed the clinical data of 71 children with leukemia treated using allo-HSCT to observe the clinical efficacy and analyze the possible influencing factors.
基金Supported by the Science and Technology Plan of the State Ministry of Science and Technology(No 2011BAE01B17).
文摘We demonstrate that the Mg-doping in barriers can partially screen the polarization fields of InGaN-based green light-emitting diodes.The photocurrent spectra show that the Mg-doping samples have smaller polarization fields and the blue shift of the peak with increasing current is observed.The reduction of polarization fields can be attributed to the screening of the impurity holes generated by the Mg atoms in the barriers.The efficiency droop is sensitive to the Mg-doping concentration in barriers,while the sample with Mg concentration of 5×10^(19) cm^(-3) exhibits the lowest efficiency degradation of 12.4%at a high injection current.
