Background It is accepted that inflammatory cytokines play a key role in the development of acute pancreatitis, so blocking the initiation of inflammatory reactions may alleviate pathological changes of acute pancreat...Background It is accepted that inflammatory cytokines play a key role in the development of acute pancreatitis, so blocking the initiation of inflammatory reactions may alleviate pathological changes of acute pancreatitis. We studied the regulatory effect of arsenic trioxide （As2O3） on apoptosis and oncosis of pancreatic acinar cells in vitro and in vivo and its therapeutic effect on acute pancreatitis. Methods Pancreatic acinar cells were isolated by collagenase digestion method. Apoptosis and oncosis of isolated pancreatic acinar cells were detected with Hoechst 33258＋PI or Annexin V＋PI double fluorescent staining. Amylase and lactate dehydrogenase release were measured. Acute pancreatitis was induced in Wistar rats by intraperitoneal injections of caerulein, and apoptosis was detected with terminal dUTP nick-end labeling method. Tumor necorsis factor α （TNF-α） mRNA, myeloperoxidase, nuclear factor-κB and histological grading of pancreatic damage were measured.Results There was an increased apoptosis but a decreased oncosis of pancreatic acinar cell after the treatment with AS2O3. The levels of lactate dehydrogenase and amylase release were markedly decreased in As2O3 treated group. Myeloperoxidase content, TNF-α mRNA level, nuclear factor-κB activation and pathological score in As2O3 treated group were significantly lower than in the untreated group. Conclusions As2O3 can induce apoptosis and reduce oncosis of pancreatic acinar cell, thus resulting in reduced release of endocellular enzyme of acinar cells, reduced inflammatory cell infiltration and decreased the production of inflammatory cytokines, so that the outcome of alleviated pathological changes was finally achieved.展开更多
Schistosomiasis japonica,caused by the human blood fluke Schistosoma japonicum,remains a major public health problem in China,although great success has been achieved.The control efforts during the past half-decade,no...Schistosomiasis japonica,caused by the human blood fluke Schistosoma japonicum,remains a major public health problem in China,although great success has been achieved.The control efforts during the past half-decade,notably the wide implementation of the new integrated strategy with emphasis on control of the source of S.japonicum infection across the country since 2004,has greatly reduced S.japonicum in humans,livestock,and intermediate host Oncomelania hupensis snails,and transmission control of schistosomiasis was achieved in China in 2015.A two-stage roadmap was therefore proposed for schistosomiasis elimination in 2015,with aims to achieve transmission interruption by 2020 and achieve disease elimination by 2025 in the country.During the last two decades,a variety of approaches,which target the epidemiological factors of schistosomiasis japonica have been developed,in order to block the transmission cycle of the parasite.These approaches have been employed in the national or local schistosomiasis control activities,and facilitated,at least in part,the progress of the schistosomiasis elimination programs.Here,we present an approach to control the source of S.japonicum infection,three new tools for snail control,three approaches for detecting and monitoring S.japonicum infection,and a novel model for health education.These approaches are considered to play a great role in the stage moving towards transmission interruption and elimination of schistosomiasis in China.展开更多
流域土壤侵蚀预测对于了解未来土壤侵蚀发展趋势,制定未来水土保持治理策略具有重要意义。为了提出一种适用于黄土高原地区的易于评估未来不同土地利用管理策略的土壤侵蚀预测方法,该研究基于地形、降雨、土壤、遥感影像数据,完成韭园...流域土壤侵蚀预测对于了解未来土壤侵蚀发展趋势,制定未来水土保持治理策略具有重要意义。为了提出一种适用于黄土高原地区的易于评估未来不同土地利用管理策略的土壤侵蚀预测方法,该研究基于地形、降雨、土壤、遥感影像数据,完成韭园沟流域2010—2020年的土地利用空间分布解译,并计算历史时期(2010—2020)的土壤侵蚀模数,基于未来土地利用模拟(Future Land Use Simulation,FLUS)模型完成流域2025年土地利用分布状况预测,以此为基础获得未来植被覆盖措施因子和耕作措施因子,结合CSLE模型预测2025年自然发展、经济增长、生态保护3种不同土地利用变化情景下土壤侵蚀状况。结果表明:1)韭园沟流域土地利用类型主要为草地(面积占比62.23%)和林地(28.41%),其次是耕地、建筑物和水体,在2010—2020年期间土地利用空间分布格局经历了较大变化,林、草地面积增加8.36%,耕地面积减少30.3%。2)流域2010、2015、2020年这3a间土壤侵蚀模数平均值分别为19.49、15.83、20.7t/(hm^(2)·a),整体呈现先降低后增加的趋势,不同土地利用类型的土壤侵蚀模数由大到小为耕地(40.56t/(hm^(2)·a))、草地(18.79 t/(hm^(2)·a))、建设用地(10.25 t/(hm^(2)·a))、林地(8.02 t/(hm^(2)·a))。3)在积极的生态保护情景下,2025年林、草地面积较自然发展情景基本持平但林地面积比例有所增加,较经济增长情景林、草地面积增加5.06%,耕地面积较自然发展情景增加1.20%,较经济增长情景减少14.73%。4)2025年流域自然发展、经济增长、生态保护情景下土壤侵蚀模数分别为24.3、22.9、18.3 t/(hm^(2)·a)。采取积极的生态保护情景发展模式,建设用地面积适度扩张可以兼顾生态保护和经济发展的需要。该研究为流域未来的土地利用规划以及水土保持治理提供参考。展开更多
AIM： To comprehensively analyze the risk factors of rhegmatogenous retinal detachment （RRD） associated with choroidal detachment （CD）. METHODS： A total of 265 eyes of 265 consecutive cases of RRD were retrospec...AIM： To comprehensively analyze the risk factors of rhegmatogenous retinal detachment （RRD） associated with choroidal detachment （CD）. METHODS： A total of 265 eyes of 265 consecutive cases of RRD were retrospectively analyzed. All patients had systemic and ophthalmologic examination. CD was diagnosed by indirect ophthalmoscopy, B -scan ultrasonography, and ultrasound biomicroscope （UBM）. Each parameter was compared between patients of RRD and rhegmatogenous retinal detachment associated with choroidal detachment （RRDCD）. Logistic regression analysis was used to determine the independent risk factors of CD. RESULTS： There were 52 eyes （19.62%） with CD. Pseudophakia was more commonly seen in RRDCD （21.15% ys6.10%, P=0.002）. Intraocular pressure （lOP） was lower （8.60 ±3.62 ys 12.96 ±3.55, P 〈0.001）, best - corrected visual acuity was worse [3.00 （2.00 to 3.00） vs 1.92 （1.22 to 3.00）, P=-0.001], and refractive error was more myopic [-4 （-9 to -2） vs -2 （-6 to 0）, P=0.007] in RRDCD. Eyes with RRDCD had larger extent of retinal detachment （P=0.007）. In RRDCD, 34.62% of eyes presented with multiple holes （P=0.044） and 25.00% with macular holes （P=0.012）, compared with 20.66% and 14.08% in R RD. High myopia （P=0.039）, low lOP （P=0.017）, and larger extent of retinal detachment （P〈0.001） were significant and independent risk factors for developing CD. - CONCLUSION： For CD in RRD, related factors include BCVA, lOP, lens status, refractive error, extent of retinal detachment, number of holes, and macular hole. Larger extent of retinal detachment, high myopia, and low lOP are significant and independent risk factors.展开更多
Previous studies have indicated regional abnormalities of both gray and white matter in amblyopia. However, alterations of cortical thickness associated with changes in white matter integrity have rarely been reported...Previous studies have indicated regional abnormalities of both gray and white matter in amblyopia. However, alterations of cortical thickness associated with changes in white matter integrity have rarely been reported. In this study, structural magnetic resonance imaging and diffusion tensor imaging （DTI） data were obtained from 15 children with anisometropic amblyopia and 15 age- and gender-matched children with normal sight. Combining DTI and surface-based morphometry, we examined a potential linkage between disrupted white matter integrity and altered cortical thickness. The fractional anisotropy （FA） values in the optic radiations （ORs） of children with anisometropic amblyopia were lower than in controls （P 〈 0.05）. The cortical thickness in amblyopic children was lower than controls in the following subregions： lin- gual cortex, lateral occipitotemporal gyrus, cuneus, occip- ital lobe, inferior parietal lobe, and temporal lobe （P 〈 0.05, corrected）, but was higher in the calcarine gyrus （P 〈 0.05, corrected）. Node-by-node correlation analysis of changes in cortical thickness revealed a significant association between a lower FA value in the OR and diminished cortical thickness in the following subregions： medial lingual cortex, lateral occipitotemporal gyrus, lat- eral, superior, and medial occipital cortex, and lunate cortex. We also found a relationship between changes of cortical thickness and white matter OR integrity in amblyopia. These findings indicate that developmental changes occur simultaneously in the OR and visual cortex in amblyopia, and provide key information on complex damage of brain networks in anisometropic amblyopia. Our results also support the hypothesis that the pathogenesis of anisometropic amblyopia is neurodevelopmental.展开更多
Excess phosphorus （P） from agricultural soils contributes to eutrophication in water bodies. Samples （n = 60） were taken from sites where rice paddies have been converted to vegetable fields for 0, 〈 10, 10-20, a...Excess phosphorus （P） from agricultural soils contributes to eutrophication in water bodies. Samples （n = 60） were taken from sites where rice paddies have been converted to vegetable fields for 0, 〈 10, 10-20, and 〉 20 years and analyzed for five inorganic P （Pi） fractions, three organic P （Po） fractions, and several soil parameters to investigate how land use conversion affects Pi and Po fractions in a peri-urban area of China with soils characteristic of many agricultural areas of Asia. Significant increases of 33, 281, 293, and 438 mg kg-i were found for soluble and loosely bound Pi （SL-Pi）, aluminum-bound Pi （Al-Pi）, calcium-bound Pi （Ca-Pi）, and iron-bound Pi （Fe-Pi）, respectively, after conversion from rice paddies to vegetable fields. Most of the increase in Pi was in the form of Fe-Pi, which increased from 8% of total P （TP） on paddy soil to 31% on the soil with 〉 20-year vegetable cultivation, followed by Al-Pi, which increased from 2% to 19% of TP. For Po fractions, there was no significant change in P concentrations. The conversion of land use from paddy fields to high intensity vegetable fields was causing significant changes in soil P fractious. Management practices were causing a buildup of soil P, primarily in the Fe-Pi fraction, followed by Ca-Pi and Al-Pi fractions. If current trends continue, a 30%-70% increase in TP could be expected in the next 20 years. Farmers in the area should reduce P application and use to maximize P uptake.展开更多
基金a grant from the Provincial Natural Sciences Foundation of Heilongjiang(No.D0227)
文摘Background It is accepted that inflammatory cytokines play a key role in the development of acute pancreatitis, so blocking the initiation of inflammatory reactions may alleviate pathological changes of acute pancreatitis. We studied the regulatory effect of arsenic trioxide （As2O3） on apoptosis and oncosis of pancreatic acinar cells in vitro and in vivo and its therapeutic effect on acute pancreatitis. Methods Pancreatic acinar cells were isolated by collagenase digestion method. Apoptosis and oncosis of isolated pancreatic acinar cells were detected with Hoechst 33258＋PI or Annexin V＋PI double fluorescent staining. Amylase and lactate dehydrogenase release were measured. Acute pancreatitis was induced in Wistar rats by intraperitoneal injections of caerulein, and apoptosis was detected with terminal dUTP nick-end labeling method. Tumor necorsis factor α （TNF-α） mRNA, myeloperoxidase, nuclear factor-κB and histological grading of pancreatic damage were measured.Results There was an increased apoptosis but a decreased oncosis of pancreatic acinar cell after the treatment with AS2O3. The levels of lactate dehydrogenase and amylase release were markedly decreased in As2O3 treated group. Myeloperoxidase content, TNF-α mRNA level, nuclear factor-κB activation and pathological score in As2O3 treated group were significantly lower than in the untreated group. Conclusions As2O3 can induce apoptosis and reduce oncosis of pancreatic acinar cell, thus resulting in reduced release of endocellular enzyme of acinar cells, reduced inflammatory cell infiltration and decreased the production of inflammatory cytokines, so that the outcome of alleviated pathological changes was finally achieved.
基金This study was supported by the grants from the China UK Global Health Support Programme(grant no.GHSP-OP202)National S&T Major Program(grant no.2012ZX10004-220)+3 种基金National Science&Technology Pillar Program of China(grant no.2009BAI78B06)Shanghai Public Health 3-Year Action Plan(grant no.15GWZK0101)Jiangsu Provincial Science&Technology Project(grant no.BL2014021),Jiangsu Provincial Young Talents in Medical Sciences(grant no.QNRC2016621)Jiangsu Department of Health(grant nos.Q201404 and X201410)。
文摘Schistosomiasis japonica,caused by the human blood fluke Schistosoma japonicum,remains a major public health problem in China,although great success has been achieved.The control efforts during the past half-decade,notably the wide implementation of the new integrated strategy with emphasis on control of the source of S.japonicum infection across the country since 2004,has greatly reduced S.japonicum in humans,livestock,and intermediate host Oncomelania hupensis snails,and transmission control of schistosomiasis was achieved in China in 2015.A two-stage roadmap was therefore proposed for schistosomiasis elimination in 2015,with aims to achieve transmission interruption by 2020 and achieve disease elimination by 2025 in the country.During the last two decades,a variety of approaches,which target the epidemiological factors of schistosomiasis japonica have been developed,in order to block the transmission cycle of the parasite.These approaches have been employed in the national or local schistosomiasis control activities,and facilitated,at least in part,the progress of the schistosomiasis elimination programs.Here,we present an approach to control the source of S.japonicum infection,three new tools for snail control,three approaches for detecting and monitoring S.japonicum infection,and a novel model for health education.These approaches are considered to play a great role in the stage moving towards transmission interruption and elimination of schistosomiasis in China.
文摘流域土壤侵蚀预测对于了解未来土壤侵蚀发展趋势,制定未来水土保持治理策略具有重要意义。为了提出一种适用于黄土高原地区的易于评估未来不同土地利用管理策略的土壤侵蚀预测方法,该研究基于地形、降雨、土壤、遥感影像数据,完成韭园沟流域2010—2020年的土地利用空间分布解译,并计算历史时期(2010—2020)的土壤侵蚀模数,基于未来土地利用模拟(Future Land Use Simulation,FLUS)模型完成流域2025年土地利用分布状况预测,以此为基础获得未来植被覆盖措施因子和耕作措施因子,结合CSLE模型预测2025年自然发展、经济增长、生态保护3种不同土地利用变化情景下土壤侵蚀状况。结果表明:1)韭园沟流域土地利用类型主要为草地(面积占比62.23%)和林地(28.41%),其次是耕地、建筑物和水体,在2010—2020年期间土地利用空间分布格局经历了较大变化,林、草地面积增加8.36%,耕地面积减少30.3%。2)流域2010、2015、2020年这3a间土壤侵蚀模数平均值分别为19.49、15.83、20.7t/(hm^(2)·a),整体呈现先降低后增加的趋势,不同土地利用类型的土壤侵蚀模数由大到小为耕地(40.56t/(hm^(2)·a))、草地(18.79 t/(hm^(2)·a))、建设用地(10.25 t/(hm^(2)·a))、林地(8.02 t/(hm^(2)·a))。3)在积极的生态保护情景下,2025年林、草地面积较自然发展情景基本持平但林地面积比例有所增加,较经济增长情景林、草地面积增加5.06%,耕地面积较自然发展情景增加1.20%,较经济增长情景减少14.73%。4)2025年流域自然发展、经济增长、生态保护情景下土壤侵蚀模数分别为24.3、22.9、18.3 t/(hm^(2)·a)。采取积极的生态保护情景发展模式,建设用地面积适度扩张可以兼顾生态保护和经济发展的需要。该研究为流域未来的土地利用规划以及水土保持治理提供参考。
文摘AIM： To comprehensively analyze the risk factors of rhegmatogenous retinal detachment （RRD） associated with choroidal detachment （CD）. METHODS： A total of 265 eyes of 265 consecutive cases of RRD were retrospectively analyzed. All patients had systemic and ophthalmologic examination. CD was diagnosed by indirect ophthalmoscopy, B -scan ultrasonography, and ultrasound biomicroscope （UBM）. Each parameter was compared between patients of RRD and rhegmatogenous retinal detachment associated with choroidal detachment （RRDCD）. Logistic regression analysis was used to determine the independent risk factors of CD. RESULTS： There were 52 eyes （19.62%） with CD. Pseudophakia was more commonly seen in RRDCD （21.15% ys6.10%, P=0.002）. Intraocular pressure （lOP） was lower （8.60 ±3.62 ys 12.96 ±3.55, P 〈0.001）, best - corrected visual acuity was worse [3.00 （2.00 to 3.00） vs 1.92 （1.22 to 3.00）, P=-0.001], and refractive error was more myopic [-4 （-9 to -2） vs -2 （-6 to 0）, P=0.007] in RRDCD. Eyes with RRDCD had larger extent of retinal detachment （P=0.007）. In RRDCD, 34.62% of eyes presented with multiple holes （P=0.044） and 25.00% with macular holes （P=0.012）, compared with 20.66% and 14.08% in R RD. High myopia （P=0.039）, low lOP （P=0.017）, and larger extent of retinal detachment （P〈0.001） were significant and independent risk factors for developing CD. - CONCLUSION： For CD in RRD, related factors include BCVA, lOP, lens status, refractive error, extent of retinal detachment, number of holes, and macular hole. Larger extent of retinal detachment, high myopia, and low lOP are significant and independent risk factors.
基金supported by the National Natural Science Foundation of China(30872717)
文摘Previous studies have indicated regional abnormalities of both gray and white matter in amblyopia. However, alterations of cortical thickness associated with changes in white matter integrity have rarely been reported. In this study, structural magnetic resonance imaging and diffusion tensor imaging （DTI） data were obtained from 15 children with anisometropic amblyopia and 15 age- and gender-matched children with normal sight. Combining DTI and surface-based morphometry, we examined a potential linkage between disrupted white matter integrity and altered cortical thickness. The fractional anisotropy （FA） values in the optic radiations （ORs） of children with anisometropic amblyopia were lower than in controls （P 〈 0.05）. The cortical thickness in amblyopic children was lower than controls in the following subregions： lin- gual cortex, lateral occipitotemporal gyrus, cuneus, occip- ital lobe, inferior parietal lobe, and temporal lobe （P 〈 0.05, corrected）, but was higher in the calcarine gyrus （P 〈 0.05, corrected）. Node-by-node correlation analysis of changes in cortical thickness revealed a significant association between a lower FA value in the OR and diminished cortical thickness in the following subregions： medial lingual cortex, lateral occipitotemporal gyrus, lat- eral, superior, and medial occipital cortex, and lunate cortex. We also found a relationship between changes of cortical thickness and white matter OR integrity in amblyopia. These findings indicate that developmental changes occur simultaneously in the OR and visual cortex in amblyopia, and provide key information on complex damage of brain networks in anisometropic amblyopia. Our results also support the hypothesis that the pathogenesis of anisometropic amblyopia is neurodevelopmental.
基金Project supported by the National Science & Technology Pillar Program of China during the Eleventh Five-Year Plan Period (No. 2008BADA7B02)the National Natural Science Foundation of China (No. 40773075)the Natural Science Foundation of Jiangsu Province,China (No. BK2007262)
文摘Excess phosphorus （P） from agricultural soils contributes to eutrophication in water bodies. Samples （n = 60） were taken from sites where rice paddies have been converted to vegetable fields for 0, 〈 10, 10-20, and 〉 20 years and analyzed for five inorganic P （Pi） fractions, three organic P （Po） fractions, and several soil parameters to investigate how land use conversion affects Pi and Po fractions in a peri-urban area of China with soils characteristic of many agricultural areas of Asia. Significant increases of 33, 281, 293, and 438 mg kg-i were found for soluble and loosely bound Pi （SL-Pi）, aluminum-bound Pi （Al-Pi）, calcium-bound Pi （Ca-Pi）, and iron-bound Pi （Fe-Pi）, respectively, after conversion from rice paddies to vegetable fields. Most of the increase in Pi was in the form of Fe-Pi, which increased from 8% of total P （TP） on paddy soil to 31% on the soil with 〉 20-year vegetable cultivation, followed by Al-Pi, which increased from 2% to 19% of TP. For Po fractions, there was no significant change in P concentrations. The conversion of land use from paddy fields to high intensity vegetable fields was causing significant changes in soil P fractious. Management practices were causing a buildup of soil P, primarily in the Fe-Pi fraction, followed by Ca-Pi and Al-Pi fractions. If current trends continue, a 30%-70% increase in TP could be expected in the next 20 years. Farmers in the area should reduce P application and use to maximize P uptake.
