Objective:To observe the effects of electroacupuncture(EA)on type 2 innate lymphoid cell(ILC2)function in white adipose tissue(WAT)of high-fat diet(HFD)-induced obese rats to reveal the mechanism underlying weight los...Objective:To observe the effects of electroacupuncture(EA)on type 2 innate lymphoid cell(ILC2)function in white adipose tissue(WAT)of high-fat diet(HFD)-induced obese rats to reveal the mechanism underlying weight loss.Methods:Male Winstar rats(n=15)were randomly divided into control,HFD,and HFD+EA groups,with five rats in each group.An obesity model was established by feeding rats with a HFD.EA(2 Hz,1 mA)was applied to"Zhongwan(CV12)"bilateral"Tianshu(ST25)""Zusanli(ST36)"and"Sanyinjiao(SP6)"for15 min,7 days a week,for a total of 5 weeks.During EA treatment,the HFD group was fed a HFD,whereas rats in the control group were maintained on a normal diet.There was no intervention in either the control or the HFD groups.Body weight was measured weekly.Full-field adipocyte morphology,adipocyte volume,and the number of abdominal WAT were observed under a microscope after sectioning and hematoxylin and eosin staining.The levels of IL-5(interleukin-5),IL-13(interleukin-13),IL-33(interleukin-33),and ST2(growth stimulating gene 2 protein)in the WAT were assayed using enzymelinked immunosorbent assay and reverse transcription-polymerase chain reaction(RT-PCR).The expression levels of browning markers including PGClα(peroxisome proliferator-activated receptor gamma coactivator 1-alpha),PPARγ(peroxisome proliferator-activated receptor gamma),PRDM16(histone-lysine Nmethyltransferase),and UCP1(uncoupling protein 1 in WAT were detected by western blot analysis and RT-PCR.Results:After a 7-week induction period,rats in both the HFD and HFD+EA groups were 20%heavier than their initial weights before modeling(P<0.01,P<0.001).Following a 5-week EA intervention,the body mass and Lee index of obese rats were significantly reduced(P<0.05).The HE results showed that adipocytes in the HFD group displayed a significant increase in diameter and a decrease in number compared to the control group.However,in the HFD+EA group,white adipocytes exhibited reduced diameter and appeared more regular in shape.In the control group,the proportion of展开更多
基金Supported by the State Key Laboratory of Dampness Syndrome of Chinese Medicine Special Fund:SZ2021ZZ08the Guangdong Provincial Bureau of Chinese Medicine:20233013the Guangdong Provincial Hospital of Chinese Medicine Fund:YN2020MS02,YN2019ZWB01,YN2019ML01。
文摘Objective:To observe the effects of electroacupuncture(EA)on type 2 innate lymphoid cell(ILC2)function in white adipose tissue(WAT)of high-fat diet(HFD)-induced obese rats to reveal the mechanism underlying weight loss.Methods:Male Winstar rats(n=15)were randomly divided into control,HFD,and HFD+EA groups,with five rats in each group.An obesity model was established by feeding rats with a HFD.EA(2 Hz,1 mA)was applied to"Zhongwan(CV12)"bilateral"Tianshu(ST25)""Zusanli(ST36)"and"Sanyinjiao(SP6)"for15 min,7 days a week,for a total of 5 weeks.During EA treatment,the HFD group was fed a HFD,whereas rats in the control group were maintained on a normal diet.There was no intervention in either the control or the HFD groups.Body weight was measured weekly.Full-field adipocyte morphology,adipocyte volume,and the number of abdominal WAT were observed under a microscope after sectioning and hematoxylin and eosin staining.The levels of IL-5(interleukin-5),IL-13(interleukin-13),IL-33(interleukin-33),and ST2(growth stimulating gene 2 protein)in the WAT were assayed using enzymelinked immunosorbent assay and reverse transcription-polymerase chain reaction(RT-PCR).The expression levels of browning markers including PGClα(peroxisome proliferator-activated receptor gamma coactivator 1-alpha),PPARγ(peroxisome proliferator-activated receptor gamma),PRDM16(histone-lysine Nmethyltransferase),and UCP1(uncoupling protein 1 in WAT were detected by western blot analysis and RT-PCR.Results:After a 7-week induction period,rats in both the HFD and HFD+EA groups were 20%heavier than their initial weights before modeling(P<0.01,P<0.001).Following a 5-week EA intervention,the body mass and Lee index of obese rats were significantly reduced(P<0.05).The HE results showed that adipocytes in the HFD group displayed a significant increase in diameter and a decrease in number compared to the control group.However,in the HFD+EA group,white adipocytes exhibited reduced diameter and appeared more regular in shape.In the control group,the proportion of
基金supported by the National Natural Science Foundation of China(No.52004076)the Science and Technology Base and Talent Project of Guangxi,China(No.GUIKEAD20159049)the Guangxi Natural Science Foundation,China(No.2020GXNSFAA297054)。
