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BLOC1S1促进山羊精原干细胞增殖 被引量:2
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作者 万仕成 张梦菲 +9 位作者 陈文博 韩苗 杨栋慧 王聪亮 吴文萍 王瑜琪 李娜 朱海鲸 ahmed hamed Arisha 华进联 《生物工程学报》 CAS CSCD 北大核心 2023年第12期4901-4914,共14页
随着基因编辑技术迅速发展,研究精原干细胞(spermatogonial stem cells,SSCs)对精子发生及其调控机制研究、转基因动物研发、基因治疗和不孕不育症的治疗以及珍稀物种的保护均具有重要意义。溶酶体相关细胞器生物合成复合体1亚基1(bioge... 随着基因编辑技术迅速发展,研究精原干细胞(spermatogonial stem cells,SSCs)对精子发生及其调控机制研究、转基因动物研发、基因治疗和不孕不育症的治疗以及珍稀物种的保护均具有重要意义。溶酶体相关细胞器生物合成复合体1亚基1(biogenesis of lysosome-related organelles complex 1subunit 1,BLOC1S1)具有抗布鲁氏菌的潜能,研究BLOC1S1对山羊SSCs的影响不仅能探究BLOC1S1促进SSCs增殖的能力,还能为其抗病育种研究提供细胞学基础。本研究通过同源重组构建BLOC1S1过表达载体,通过慢病毒包装、转染与嘌呤霉素筛选成功构建了山羊精原干细胞BLOC1S1过表达细胞株。通过实时荧光定量PCR(real time quantitative PCR,RT-qPCR)检测BLOC1S1的过表达效率为18倍,同时细胞生长曲线、流式细胞术检测细胞周期、5-乙炔基-2’脱氧尿嘧啶核苷(5-ethynyl-2’-deoxyuridine,EdU)染色等实验结果表明,BLOC1S1能显著增加山羊SSCs的增殖活性。RT-qPCR、免疫荧光染色、Western blotting结果显示与增殖相关的基因(PCNA、CDK2、CCND1)上调,同时调控精原干细胞增殖的关键基因EIF2S3Y的表达量也上调。本研究成功构建了山羊精原干细胞BLOC1S1过表达细胞株,提高其增殖能力,并且这种促增殖能力可能是通过EIF2S3Y/ERK通路实现的。本研究为探究BLOC1S1对山羊精原干细胞的调控作用奠定了细胞学基础,并为进一步研究BLOC1S1的生物学功能提供细胞平台,为繁育BLOC1S1修饰抗病山羊奠定了基础。 展开更多
关键词 山羊 溶酶体相关细胞器生物合成复合体1亚基1(BLOC1S1) 精原干细胞 增殖
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Wear Characteristics of Functionally Graded Tubes Reinforced with Silicon Carbide and Alumina: A Comparative Study
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作者 SALEH Bassiouny Ibrahim ahmed Mahmoud hamed 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2021年第S01期76-83,共8页
In the current study,an effort has been taken to manufacture and compare the characteristics of functionally graded tubes. Two samples have been produced with a commercially pure aluminium matrix reinforced by 10%(in ... In the current study,an effort has been taken to manufacture and compare the characteristics of functionally graded tubes. Two samples have been produced with a commercially pure aluminium matrix reinforced by 10%(in weight) SiC and Al_(2)O_(3)with a 16 μm average size via horizontal centrifugal casting. In addition to microstructure analysis,the mechanical and wear characteristics have been investigated across the thickness of the produced tubes.The results showed that the addition of SiC and Al_(2)O_(3)particles relative to the unreinforced matrix(pure Al)significantly enhance the mechanical properties and wear resistance of the produced tubes. Thus,these tubes are the best choice for automobile applications. 展开更多
关键词 functionally graded materials centrifugal casting pure aluminium wear characteristics
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