Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our k...Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease diagnosis.In this review,we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in vivo.In doing so,we highlight the importance of each biological species and their role in biological systems and for disease progression.We then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research area.Overall,we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.展开更多
基金the National Natural Science Foundation of China(21788102,91853201)the Shanghai Municipal Science and Technology Major Project(2018SHZDZX03)+9 种基金the International Cooperation Program of Shanghai Science and Technology Committee(17520750100)the Projects from the Shanghai Science and Techonology Commission(19441905000)the Fundamental Research Funds for the Central Universities(222201717003)the Programme of Introducing Talents of Discipline to Universities(B16017)for financial supportthe National Natural Science Foundation of China(22107028)National Postdoctoral Program for Innovative Talents(BX20190115)Shanghai Post-doctoral Excellence Program(2019044)China Postdoctoral Science Foundation(2020M681206)for financial supportthe Project funded by China Postdoctoral Science Foundation(2020M681196)the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering,Henan Normal University for support(2020ZD01)。
文摘Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease diagnosis.In this review,we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in vivo.In doing so,we highlight the importance of each biological species and their role in biological systems and for disease progression.We then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research area.Overall,we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.
