Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control.Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as ...Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control.Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against Meloidogyne incognita.Among the 294 isolates screened,23 significantly reduced galls formed by M.incognita in greenhouse test.The 10 most effective isolates were Fusarium(5),Trichoderma(1),Chaetomium(1),Acremonium(1),Paecilomyces(1),and Phyllosticta(1) .Their control efficacies were repeatedly tested and their colonizations as well as in vitro activity against M.incognita were studied.They reduced the number of galls by 24.0%-58.4%in the first screening and 15.6%-44.3%in the repeated test,respectively.Phyllosticta Ph511 and Chaetomium Ch1001 had high colonizations on both the roots and the aboveground parts of cucumber seedlings.Fusarium isolates had colonization preference on the roots,their root colonizations ranging from 20.1%to 47.3%of the total root area.Trichoderma Tr882,Paecilomyces Pa972,and Acremonium Ac985 had low colonizations on both the roots and the aboveground parts.Acremonium Ac985,Chaetomium Ch1001,Paecilomyces Pa972,and Phyllosticta Ph511 produced compounds affecting motility of the second stage juveniles of M.incognita.Based on these results,Chae-tomium Ch1001 was considered to have the highest potential as a seed treatment agent for M.incognita biocontrol.展开更多
With a unique and large size of testing results of 1,842 samples collected from 12 wastewater treatment plants (WWTP) for 14 months through from low to high prevalence of COVID-19,the sensitivity of RT-qPCR detection ...With a unique and large size of testing results of 1,842 samples collected from 12 wastewater treatment plants (WWTP) for 14 months through from low to high prevalence of COVID-19,the sensitivity of RT-qPCR detection of SARS-CoV-2 RNA in wastewater that correspond to the communities was computed by using Probit analysis.This study determined the number of new COVID-19 cases per 100,000 population required to detect SARS-CoV-2RNA in wastewater at defined probabilities and provided an evidence-based framework of wastewater-based epidemiology surveillance (WBE).Input data were positive and negative test results of SARS-CoV-2 RNA in wastewater samples and the corresponding new COVID-19 case rates per 100,000 population served by each WWTP.The analyses determined that RT-qPCR-based SARS-CoV-2 RNA detection threshold at 50%,80%and 99%probability required a median of 8 (range:4-19),18 (9-43),and 38 (17-97) of new COVID-19 cases/100,000,respectively.Namely,the positive detection rate at 50%,80%and 99%probability were 0.01%,0.02%,and 0.04%averagely for new cases in the population.This study improves understanding of the performance of WBE SARS-CoV-2 RNA detection using the large datasets and prolonged study period.Estimated COVID-19 burden at a community level that would result in a positive detection of SARS-CoV-2 in wastewater is critical to support WBE application as a supplementary warning/monitoring system for COVID-19 prevention and control.展开更多
Binomial N-mixture models are commonly applied to estimate abundance unaffected by imperfect detection,but are known to be sensitive to violations of assumptions.One of the model’s assumptions,the independence of det...Binomial N-mixture models are commonly applied to estimate abundance unaffected by imperfect detection,but are known to be sensitive to violations of assumptions.One of the model’s assumptions,the independence of detections has rarely been tested.It requires that during a survey,detection of one individual does not affect detection of another individual.This assumption can be frequently violated in passerine birds,which exhibit territorial behaviour by singing,since neighbouring individuals are likely to motivate each other to vocalize,leading to non-independence in singing activity and in the following detection rate.Here,we explored this phenomenon with the generalized,binomial version of the N-mixture model,where detection probability is decomposed into availability probability φ-which can be interpreted as per capita song rate or the probability of vocalising-and actual detection probability p,given vocalisations take place.Using repeated counts of the Collared Flycatcher(Ficedula albicollis)as a case study,and treating the maximum observed counts C_(maxi) at a site i as an explanatory covariate for φ,we showed that per capita song rates increased with observed counts at a site.Hence,if song rates vary due to local abundance,including C_(maxi)as an explanatory variable for song rate addressed with φ,helps to explain this variation(which otherwise goes undetected)and improves inferences under the model.This had strong effects on the resulting abundance estimates:if this relationship was ignored in the models,total estimated population sizes were consequently lower by as much as 22-27%,compared to when this effect was included.Since it is likely that song rates may commonly be density-dependent in birds manifesting territorial behaviours by singing,further tests addressing violations of independence assumptions in these models are needed.As suggested by Kery and Royle(2016),despite some form of circularity likely being involved,modelling heterogeneity in the detection process with the help of C_(maxi)in st展开更多
In order to determine the level of resistance of sugar beet varieties against Rhizoctonia solani AG 2-21IIB and AG 4, a methodology was implemented under greenhouse conditions that contemplated the most important crit...In order to determine the level of resistance of sugar beet varieties against Rhizoctonia solani AG 2-21IIB and AG 4, a methodology was implemented under greenhouse conditions that contemplated the most important criteria regarding to plant-pathogen interaction. The effect of plant growth stage on the development of the disease was evaluated. Seven sugar beet varieties were tested for resistance to R. solani AG 2-2IIIB and AG 4. To detect differences in leaf temperature between/L solani inoculated plants and non-infected plants, an infrared （IR） camera was tested. High incidence of R. solani AG 2-2IIIB and AG 4 in sugar beet plants was evident when the fungal inoculum was applied to two and four weeks old plants. At four weeks after sowing, it was the optimum time to inoculate sugar beet plants in order to generate R. solani infection, since at this time all plants were infected. Significant differences were detected regarding disease incidence between sugar beet varieties inoculated with different anastomosis groups. Leaf temperature was significant different between inoculated and non-inoculated plants, demonstrated that this technique could be a new tool for breeders to screen for resistance of new varieties.展开更多
Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still th...Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still the gold standard methods for the quantification of protein impurities.However,this technique has several limitations and does,among others,not enable the precise identification of proteins.In this context,mass spectrometry(MS)became an alternative and orthogonal method that delivers qualitative and quantitative information on all identified HCPs.However,in order to be routinely implemented in biopharmaceutical companies,liquid chromatography-MS based methods still need to be standardized to provide highest sensitivity and robust and accurate quantification.Here,we present a promising MS-based analytical workflow coupling the use of an innovative quantification standard,the HCP Profiler solution,with a spectral library-based data-independent acquisition(DIA)method and strict data validation criteria.The performances of the HCP Profiler solution were compared to more conventional standard protein spikes and the DIA approach was benchmarked against a classical datadependent acquisition on a series of samples produced at various stages of the manufacturing process.While we also explored spectral library-free DIA interpretation,the spectral library-based approach still showed highest accuracy and reproducibility(coefficients of variation<10%)with a sensitivity down to the sub-ng/mg mAb level.Thus,this workflow is today mature to be used as a robust and straightforward method to support mAb manufacturing process developments and drug products quality control.展开更多
The results of our research on the cuticular and internal lipids of Blattella germanica males provide new information on variation in the composition of the cuticular and internal lipids ofB. germanica males after exp...The results of our research on the cuticular and internal lipids of Blattella germanica males provide new information on variation in the composition of the cuticular and internal lipids ofB. germanica males after exposure to the presence of the insecticide. gas chromatography and gas chromatography-mass spectrometry analyses were used to identify and quantify the cuticular and internal lipid composition in males and males exposed to insecticide. There were significantly more acids having an even number of carbon atoms in the molecule, and these were also generally in higher concentrations. The following acids were in a higher concentration： C16：0 and C18：1, C18：2, C18：0. In both males and males exposed to insecticide, 24 fatty acids ranging from C6 to C22 were determined. However, there was a significantly higher content of fatty acids in the surface lipids of B. germanica males after exposure to insecticide. Our results indicate a higher content of n-alkanes, sterols, particularly cholesterol, fatty acids, and fatty acid methyl esters in the B. germanica surface after exposure to chlorpyrifos than in males that were not exposed.展开更多
Aim:Patients with Ehlers-Danlos Syndrome(EDS)are considered to have an increased risk for wound healing complications.Surgeons may therefore be hesitant to offer elective surgeries,including gender-affirming surgeries...Aim:Patients with Ehlers-Danlos Syndrome(EDS)are considered to have an increased risk for wound healing complications.Surgeons may therefore be hesitant to offer elective surgeries,including gender-affirming surgeries(GAS),to EDS patients.At our center,we frequently encountered patients presenting for GAS evaluation with the co-existing diagnosis of EDS.This study aims to establish the prevalence of EDS diagnosis in our GAS patients and compare their post-operative complications to patients without EDS diagnosis.Methods:This is a single-institution retrospective case-control study on all patients who underwent GAS from 2016-2020.Data include EDS diagnosis,demographics,operation,and complications(including minor wound healing issues).Results:Of 1363 patients presenting for GAS,36(2.6%)had EDS diagnoses and were matched with 108 control patients.Major complications requiring surgical intervention in the OR occurred in 6 patients(4.2%),(2.8% EDS vs.5.4%controls;P=0.63),while 8.3% of EDS and 14% of controls required minor interventions(P=0.38).The rate of wound healing issues of any severity was 28% in EDS vs.47% in control groups(P=0.04).Conclusion:The prevalence of EDS diagnosis in our patient population is 132 times the highest reported prevalence in the general population.Wound healing issues and the need for additional post-operative interventions in the group with EDS diagnosis were not significantly different from the control group.Our findings suggest that patients with a diagnosis of EDS undergoing GAS have comparable outcomes to patients without EDS.Concerns for post-operative complications should not be a barrier to offering GAS to patients presenting with an EDS diagnosis.展开更多
There is a need to standardize methods associated with the dispersion of nanomaterials in nano（eco）toxicological investigations. The first step toward this goal is to understand the degree of variability that exists...There is a need to standardize methods associated with the dispersion of nanomaterials in nano（eco）toxicological investigations. The first step toward this goal is to understand the degree of variability that exists in nanomaterial dispersions prepared by using different protocols. Using two case studies, the degree of variability in TiO2 nanomaterial dispersions was assessed by differential centrifugal sedimentation （DCS） methods. Case study 1 addresses the variability that arises from variations within one protocol, investigating the effects of dispersion aging, sonication exposure time, sonication in the presence/absence of an ice bath, material subsampling, particle concentration and having a pre-wetting step. Case study 2 addresses the variability between four different protocols developed through past research activities and projects. The results indicate that there is a large degree of variability （relative standard deviation （RSD） of mean particle diameter = 26%） in the dispersion ofTiO2 nanornaterials between the four different protocols studied and that several steps in a dispersion protocol are potential sources of variation, with final particle concentration being the most significant. The implication of this study is clear： for the purpose of data comparability, there is a need to provide the exact details of all steps involved in a dispersion protocol.展开更多
Digital networked communications are the key to all Internet-of-things applications, but especially to smart metering systems and the smart grid. In order to ensure a safe operation of systems and the privacy of users...Digital networked communications are the key to all Internet-of-things applications, but especially to smart metering systems and the smart grid. In order to ensure a safe operation of systems and the privacy of users, the transport layer security （TLS） protocol, a mature and well standardized solution for secure communications, may be used. We implemented the TLS protocol in its latest version in a way suitable for embedded and resource-constrained systems. This paper outlines the challenges and opportunities of deploying TLS in smart metering and smart grid applications and presents performance results of our TLS implementation. Our analysis shows that given an appropriate implementation and configuration, deploying TLS in constrained smart metering systems is possible with acceptable overhead.展开更多
Seemingly contradictory findings between studies are a major issue in nanoecotoxicological research and have been explained as a result of the lack of comparability between assay methods, with dispersion of nanomateri...Seemingly contradictory findings between studies are a major issue in nanoecotoxicological research and have been explained as a result of the lack of comparability between assay methods, with dispersion of nanomaterials being identified as a key factor. Here we show the use of a multivariate method, principal component analysis （PCA）, as a tool in protocol development and categorization of dispersion quality. Results show the significance of particle concentration within a protocol, and its effect on repeatability. Our results suggest that future studies should involve the use of PCA as a powerful data exploration tool to facilitate method development, comparability and integration of data across different laboratories.展开更多
Mister Chairman,Ladies and Gentlemen,dear Friends. It is a great honor and pleasure to open such an important and prominent conference as Far East Nondestructive Testing Forum.
Wireless M-Bus according to EN-13757-4 is a major contender for local metrological network （LMN） in smart metering and smart grid applications, as it holds the promise of flexible and optimized solutions. It enjoys ...Wireless M-Bus according to EN-13757-4 is a major contender for local metrological network （LMN） in smart metering and smart grid applications, as it holds the promise of flexible and optimized solutions. It enjoys wide popularity in continental Europe, but increasingly in many other regions of the world. However, Wireless M-Bus is characterized by a wide variety of different operation modes （C-, F-, N-, P-, Q-, R-, S-, and T-modes）, which work in different frequency bands （i.e., 868 MHz, 433 MHz, and 169 MHz）. Its application layer can be enhanced by extensions, being defined from vendor alliances, like the Open Metering System （OMS） Group, or from national bodies.展开更多
Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests （four from Great Britain and two from Finland） were compared using Ward＇s method with reference to： （...Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests （four from Great Britain and two from Finland） were compared using Ward＇s method with reference to： （l） similarity of DNA-random amplified microsatellite （RAMS） ladders, （2） cellulase and peroxidase production and （3） acceleration of dry mass wood loss in Norway spruce The activity of the enzymes was tested in the initial phase of wood decay （30 d after inoculation） and indicated as the most active isolates： VFI0 and FCl5 for cellulase and FC16 and VFI0 for peroxidase production. The assessment of loss of wood six months after inoculation indicated isolate FC 15 as the most active. P. gigantea isolates similar in terms of enzyme activity indicated different patterns ofDNA microsatellite loci. At the same time, DNA-RAMS revealed similarities in isolates with different abilities to produce enzymes. However, some similarities and differences between isolates according to wood decay were found. No plain relationships between molecular characteristics and enzyme activity of the strains tested were observed. The results differentiated activity of tested isolates and suggested benefits of selecting P. gigantea strains for commercial use basing mainly on the assessment of wood loss activity.展开更多
Different environmental conditions in which, equipment based on differential ion mobility spectrometry must operate, forced designers and engineers to analyze the impact of, among other things, external factors on the...Different environmental conditions in which, equipment based on differential ion mobility spectrometry must operate, forced designers and engineers to analyze the impact of, among other things, external factors on their performance and efficiency. These devices, thanks to the DMS technology, can identify and characterize the ions contained in the gaseous state. However, many areas of this technique remain undiscovered, which should be examined in more detail. One such aspect is the influence of the temperature of gaseous medium, containing tested analyte. The research presented in this article shows why temperature is an important factor on the distribution of generated peaks. The results are shown for different spectra generated carrier gas temperatures (50°C - 80°C) in which ions were toxic warfare agents. Based on those graphs, it can be stated that increase of the temperature allows for better separation of the peaks from the background. Because of the similar ion mobility of the analyte and background ions for high and low electric field, DMS device can send false alarms, due to the poor interpretations of passing a signal to them. So to be able to accurately assess the level of risk due to the presence of substances BST in air, the test medium was added to isobutanol and isopropanol additives. They help better analyze and separate measured ions.展开更多
The nucleon excitation spectrum remains poorly known, with the masses, widths, EM couplings and even existence of many states not well established. A program of experiments using meson photoproduction off the nucleon ...The nucleon excitation spectrum remains poorly known, with the masses, widths, EM couplings and even existence of many states not well established. A program of experiments using meson photoproduction off the nucleon is being carried out to improve this situation. A new large acceptance recoil polarimeter has been developed by the Edinburgh group for use in such reactions with the Crystal Ball at MAMI. This work summarizes the procedure used to measure recoil polarization with the new device and presents some preliminary results for the double polarization observable Cx in the reaction γp→pπ, compared to the current partial wave analysis.展开更多
The spectrum and properties of the excited states of the nucleon are still poorly established despite decades of study. These proceedings describe new measurements of pseudo-scalar meson photoproduction at the Crystal...The spectrum and properties of the excited states of the nucleon are still poorly established despite decades of study. These proceedings describe new measurements of pseudo-scalar meson photoproduction at the Crystal Ball at MAMI, employing a new large acceptance nucleon recoil polarimeter. The double- and single-polarisation observables obtained will provide valuable and unique data to be used as part of the world effort to improve our knowledge of this fundamental spectrum.展开更多
基金Project supported by the German Academic Exchange Agency (DAAD),the China Scholarship Council(CSC),the Special Fund for Agro-scientific Research in the Public Interest(No.nyhezx07-050-6)the Science and Technology Department of Zhejiang Province of China(No.2008C22062)the Zhejiang Provincial Natural Science Foundation of China(No.R307396)
文摘Seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control.Endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against Meloidogyne incognita.Among the 294 isolates screened,23 significantly reduced galls formed by M.incognita in greenhouse test.The 10 most effective isolates were Fusarium(5),Trichoderma(1),Chaetomium(1),Acremonium(1),Paecilomyces(1),and Phyllosticta(1) .Their control efficacies were repeatedly tested and their colonizations as well as in vitro activity against M.incognita were studied.They reduced the number of galls by 24.0%-58.4%in the first screening and 15.6%-44.3%in the repeated test,respectively.Phyllosticta Ph511 and Chaetomium Ch1001 had high colonizations on both the roots and the aboveground parts of cucumber seedlings.Fusarium isolates had colonization preference on the roots,their root colonizations ranging from 20.1%to 47.3%of the total root area.Trichoderma Tr882,Paecilomyces Pa972,and Acremonium Ac985 had low colonizations on both the roots and the aboveground parts.Acremonium Ac985,Chaetomium Ch1001,Paecilomyces Pa972,and Phyllosticta Ph511 produced compounds affecting motility of the second stage juveniles of M.incognita.Based on these results,Chae-tomium Ch1001 was considered to have the highest potential as a seed treatment agent for M.incognita biocontrol.
基金supported by research grants from the Canadian Institutes of Health Research [grant number VR5-172701, 2020]Alberta Innovates and Alberta Health [grant number RES0051047, 2020]。
文摘With a unique and large size of testing results of 1,842 samples collected from 12 wastewater treatment plants (WWTP) for 14 months through from low to high prevalence of COVID-19,the sensitivity of RT-qPCR detection of SARS-CoV-2 RNA in wastewater that correspond to the communities was computed by using Probit analysis.This study determined the number of new COVID-19 cases per 100,000 population required to detect SARS-CoV-2RNA in wastewater at defined probabilities and provided an evidence-based framework of wastewater-based epidemiology surveillance (WBE).Input data were positive and negative test results of SARS-CoV-2 RNA in wastewater samples and the corresponding new COVID-19 case rates per 100,000 population served by each WWTP.The analyses determined that RT-qPCR-based SARS-CoV-2 RNA detection threshold at 50%,80%and 99%probability required a median of 8 (range:4-19),18 (9-43),and 38 (17-97) of new COVID-19 cases/100,000,respectively.Namely,the positive detection rate at 50%,80%and 99%probability were 0.01%,0.02%,and 0.04%averagely for new cases in the population.This study improves understanding of the performance of WBE SARS-CoV-2 RNA detection using the large datasets and prolonged study period.Estimated COVID-19 burden at a community level that would result in a positive detection of SARS-CoV-2 in wastewater is critical to support WBE application as a supplementary warning/monitoring system for COVID-19 prevention and control.
基金funded by the General Directorate for Environmental Protection,Poland,within the inventory of birds in the SPA Natura 2000 Napiwodzko-Ramucka Forest。
文摘Binomial N-mixture models are commonly applied to estimate abundance unaffected by imperfect detection,but are known to be sensitive to violations of assumptions.One of the model’s assumptions,the independence of detections has rarely been tested.It requires that during a survey,detection of one individual does not affect detection of another individual.This assumption can be frequently violated in passerine birds,which exhibit territorial behaviour by singing,since neighbouring individuals are likely to motivate each other to vocalize,leading to non-independence in singing activity and in the following detection rate.Here,we explored this phenomenon with the generalized,binomial version of the N-mixture model,where detection probability is decomposed into availability probability φ-which can be interpreted as per capita song rate or the probability of vocalising-and actual detection probability p,given vocalisations take place.Using repeated counts of the Collared Flycatcher(Ficedula albicollis)as a case study,and treating the maximum observed counts C_(maxi) at a site i as an explanatory covariate for φ,we showed that per capita song rates increased with observed counts at a site.Hence,if song rates vary due to local abundance,including C_(maxi)as an explanatory variable for song rate addressed with φ,helps to explain this variation(which otherwise goes undetected)and improves inferences under the model.This had strong effects on the resulting abundance estimates:if this relationship was ignored in the models,total estimated population sizes were consequently lower by as much as 22-27%,compared to when this effect was included.Since it is likely that song rates may commonly be density-dependent in birds manifesting territorial behaviours by singing,further tests addressing violations of independence assumptions in these models are needed.As suggested by Kery and Royle(2016),despite some form of circularity likely being involved,modelling heterogeneity in the detection process with the help of C_(maxi)in st
文摘In order to determine the level of resistance of sugar beet varieties against Rhizoctonia solani AG 2-21IIB and AG 4, a methodology was implemented under greenhouse conditions that contemplated the most important criteria regarding to plant-pathogen interaction. The effect of plant growth stage on the development of the disease was evaluated. Seven sugar beet varieties were tested for resistance to R. solani AG 2-2IIIB and AG 4. To detect differences in leaf temperature between/L solani inoculated plants and non-infected plants, an infrared （IR） camera was tested. High incidence of R. solani AG 2-2IIIB and AG 4 in sugar beet plants was evident when the fungal inoculum was applied to two and four weeks old plants. At four weeks after sowing, it was the optimum time to inoculate sugar beet plants in order to generate R. solani infection, since at this time all plants were infected. Significant differences were detected regarding disease incidence between sugar beet varieties inoculated with different anastomosis groups. Leaf temperature was significant different between inoculated and non-inoculated plants, demonstrated that this technique could be a new tool for breeders to screen for resistance of new varieties.
基金supported by the“Association Nationale de la Recherche et de la Technologie”and UCB Pharma S.A.(Belgium and France)via the CIFRE fellowship of Steve Hessmannsupported by the“Agence Nationale de la Recherche”via the French Proteomic Infrastructure ProFI FR2048(ANR-10-INBS-08-03).
文摘Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still the gold standard methods for the quantification of protein impurities.However,this technique has several limitations and does,among others,not enable the precise identification of proteins.In this context,mass spectrometry(MS)became an alternative and orthogonal method that delivers qualitative and quantitative information on all identified HCPs.However,in order to be routinely implemented in biopharmaceutical companies,liquid chromatography-MS based methods still need to be standardized to provide highest sensitivity and robust and accurate quantification.Here,we present a promising MS-based analytical workflow coupling the use of an innovative quantification standard,the HCP Profiler solution,with a spectral library-based data-independent acquisition(DIA)method and strict data validation criteria.The performances of the HCP Profiler solution were compared to more conventional standard protein spikes and the DIA approach was benchmarked against a classical datadependent acquisition on a series of samples produced at various stages of the manufacturing process.While we also explored spectral library-free DIA interpretation,the spectral library-based approach still showed highest accuracy and reproducibility(coefficients of variation<10%)with a sensitivity down to the sub-ng/mg mAb level.Thus,this workflow is today mature to be used as a robust and straightforward method to support mAb manufacturing process developments and drug products quality control.
文摘The results of our research on the cuticular and internal lipids of Blattella germanica males provide new information on variation in the composition of the cuticular and internal lipids ofB. germanica males after exposure to the presence of the insecticide. gas chromatography and gas chromatography-mass spectrometry analyses were used to identify and quantify the cuticular and internal lipid composition in males and males exposed to insecticide. There were significantly more acids having an even number of carbon atoms in the molecule, and these were also generally in higher concentrations. The following acids were in a higher concentration： C16：0 and C18：1, C18：2, C18：0. In both males and males exposed to insecticide, 24 fatty acids ranging from C6 to C22 were determined. However, there was a significantly higher content of fatty acids in the surface lipids of B. germanica males after exposure to insecticide. Our results indicate a higher content of n-alkanes, sterols, particularly cholesterol, fatty acids, and fatty acid methyl esters in the B. germanica surface after exposure to chlorpyrifos than in males that were not exposed.
文摘Aim:Patients with Ehlers-Danlos Syndrome(EDS)are considered to have an increased risk for wound healing complications.Surgeons may therefore be hesitant to offer elective surgeries,including gender-affirming surgeries(GAS),to EDS patients.At our center,we frequently encountered patients presenting for GAS evaluation with the co-existing diagnosis of EDS.This study aims to establish the prevalence of EDS diagnosis in our GAS patients and compare their post-operative complications to patients without EDS diagnosis.Methods:This is a single-institution retrospective case-control study on all patients who underwent GAS from 2016-2020.Data include EDS diagnosis,demographics,operation,and complications(including minor wound healing issues).Results:Of 1363 patients presenting for GAS,36(2.6%)had EDS diagnoses and were matched with 108 control patients.Major complications requiring surgical intervention in the OR occurred in 6 patients(4.2%),(2.8% EDS vs.5.4%controls;P=0.63),while 8.3% of EDS and 14% of controls required minor interventions(P=0.38).The rate of wound healing issues of any severity was 28% in EDS vs.47% in control groups(P=0.04).Conclusion:The prevalence of EDS diagnosis in our patient population is 132 times the highest reported prevalence in the general population.Wound healing issues and the need for additional post-operative interventions in the group with EDS diagnosis were not significantly different from the control group.Our findings suggest that patients with a diagnosis of EDS undergoing GAS have comparable outcomes to patients without EDS.Concerns for post-operative complications should not be a barrier to offering GAS to patients presenting with an EDS diagnosis.
文摘There is a need to standardize methods associated with the dispersion of nanomaterials in nano（eco）toxicological investigations. The first step toward this goal is to understand the degree of variability that exists in nanomaterial dispersions prepared by using different protocols. Using two case studies, the degree of variability in TiO2 nanomaterial dispersions was assessed by differential centrifugal sedimentation （DCS） methods. Case study 1 addresses the variability that arises from variations within one protocol, investigating the effects of dispersion aging, sonication exposure time, sonication in the presence/absence of an ice bath, material subsampling, particle concentration and having a pre-wetting step. Case study 2 addresses the variability between four different protocols developed through past research activities and projects. The results indicate that there is a large degree of variability （relative standard deviation （RSD） of mean particle diameter = 26%） in the dispersion ofTiO2 nanornaterials between the four different protocols studied and that several steps in a dispersion protocol are potential sources of variation, with final particle concentration being the most significant. The implication of this study is clear： for the purpose of data comparability, there is a need to provide the exact details of all steps involved in a dispersion protocol.
基金supported in part by the Federal Ministry of Economics and Energy as a cooperative ZIM-KF project under Grant No.KF2471305ED2the good cooperation with the project partner SSV Software Systems GmbH
文摘Digital networked communications are the key to all Internet-of-things applications, but especially to smart metering systems and the smart grid. In order to ensure a safe operation of systems and the privacy of users, the transport layer security （TLS） protocol, a mature and well standardized solution for secure communications, may be used. We implemented the TLS protocol in its latest version in a way suitable for embedded and resource-constrained systems. This paper outlines the challenges and opportunities of deploying TLS in smart metering and smart grid applications and presents performance results of our TLS implementation. Our analysis shows that given an appropriate implementation and configuration, deploying TLS in constrained smart metering systems is possible with acceptable overhead.
基金Framework 7 Projects(MARINA and NanoReg)DEFRA for providing funding for this study
文摘Seemingly contradictory findings between studies are a major issue in nanoecotoxicological research and have been explained as a result of the lack of comparability between assay methods, with dispersion of nanomaterials being identified as a key factor. Here we show the use of a multivariate method, principal component analysis （PCA）, as a tool in protocol development and categorization of dispersion quality. Results show the significance of particle concentration within a protocol, and its effect on repeatability. Our results suggest that future studies should involve the use of PCA as a powerful data exploration tool to facilitate method development, comparability and integration of data across different laboratories.
文摘Mister Chairman,Ladies and Gentlemen,dear Friends. It is a great honor and pleasure to open such an important and prominent conference as Far East Nondestructive Testing Forum.
基金supported by the German Federal Ministry of Economics and Technology under Grant No.16IN0594(DEMAX project)the ARTE-MIS-JU and the Participating Member States under Grant No.100266(ME~3GAS project)the European Commission under Grant No.FP7-SME-2011-286753(WiMBex project)
文摘Wireless M-Bus according to EN-13757-4 is a major contender for local metrological network （LMN） in smart metering and smart grid applications, as it holds the promise of flexible and optimized solutions. It enjoys wide popularity in continental Europe, but increasingly in many other regions of the world. However, Wireless M-Bus is characterized by a wide variety of different operation modes （C-, F-, N-, P-, Q-, R-, S-, and T-modes）, which work in different frequency bands （i.e., 868 MHz, 433 MHz, and 169 MHz）. Its application layer can be enhanced by extensions, being defined from vendor alliances, like the Open Metering System （OMS） Group, or from national bodies.
文摘Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests （four from Great Britain and two from Finland） were compared using Ward＇s method with reference to： （l） similarity of DNA-random amplified microsatellite （RAMS） ladders, （2） cellulase and peroxidase production and （3） acceleration of dry mass wood loss in Norway spruce The activity of the enzymes was tested in the initial phase of wood decay （30 d after inoculation） and indicated as the most active isolates： VFI0 and FCl5 for cellulase and FC16 and VFI0 for peroxidase production. The assessment of loss of wood six months after inoculation indicated isolate FC 15 as the most active. P. gigantea isolates similar in terms of enzyme activity indicated different patterns ofDNA microsatellite loci. At the same time, DNA-RAMS revealed similarities in isolates with different abilities to produce enzymes. However, some similarities and differences between isolates according to wood decay were found. No plain relationships between molecular characteristics and enzyme activity of the strains tested were observed. The results differentiated activity of tested isolates and suggested benefits of selecting P. gigantea strains for commercial use basing mainly on the assessment of wood loss activity.
文摘Different environmental conditions in which, equipment based on differential ion mobility spectrometry must operate, forced designers and engineers to analyze the impact of, among other things, external factors on their performance and efficiency. These devices, thanks to the DMS technology, can identify and characterize the ions contained in the gaseous state. However, many areas of this technique remain undiscovered, which should be examined in more detail. One such aspect is the influence of the temperature of gaseous medium, containing tested analyte. The research presented in this article shows why temperature is an important factor on the distribution of generated peaks. The results are shown for different spectra generated carrier gas temperatures (50°C - 80°C) in which ions were toxic warfare agents. Based on those graphs, it can be stated that increase of the temperature allows for better separation of the peaks from the background. Because of the similar ion mobility of the analyte and background ions for high and low electric field, DMS device can send false alarms, due to the poor interpretations of passing a signal to them. So to be able to accurately assess the level of risk due to the presence of substances BST in air, the test medium was added to isobutanol and isopropanol additives. They help better analyze and separate measured ions.
文摘The nucleon excitation spectrum remains poorly known, with the masses, widths, EM couplings and even existence of many states not well established. A program of experiments using meson photoproduction off the nucleon is being carried out to improve this situation. A new large acceptance recoil polarimeter has been developed by the Edinburgh group for use in such reactions with the Crystal Ball at MAMI. This work summarizes the procedure used to measure recoil polarization with the new device and presents some preliminary results for the double polarization observable Cx in the reaction γp→pπ, compared to the current partial wave analysis.
文摘The spectrum and properties of the excited states of the nucleon are still poorly established despite decades of study. These proceedings describe new measurements of pseudo-scalar meson photoproduction at the Crystal Ball at MAMI, employing a new large acceptance nucleon recoil polarimeter. The double- and single-polarisation observables obtained will provide valuable and unique data to be used as part of the world effort to improve our knowledge of this fundamental spectrum.
