Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based...Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypo-cotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.展开更多
Lodging stress results in grain yield and quality reduction in wheat. Uniconazole, a potential plant growth regulator significantly enhances lignin biosynthesis and thus provides mechanical strength to plants in order...Lodging stress results in grain yield and quality reduction in wheat. Uniconazole, a potential plant growth regulator significantly enhances lignin biosynthesis and thus provides mechanical strength to plants in order to cope with lodging stress. A field study was conducted during the 2015–2016 and 2016–2017 growing seasons, to investigate the effects of uniconazole sole application or with micronutrient on the lignin biosynthesis, lodging resistance, and production of winter wheat. In the first experiment, uniconazole at concentrations of 0(CK), 15(US1), 30(US2), and 45(US3) mg L^-1 was applied as sole seed soaking, while in the second experiment with manganese(Mn) at concentration of 0.06 g L^-1 Mn, 0.06 g L^-1 Mn+ 15 mg L^-1 uniconazole(UMS1), 0.06 g L^-1 Mn+30 mg L^-1 uniconazole(UMS2), and 0.06 g L^-1 Mn+45 mg L^-1 uniconazole(UMS3), respectively. Uniconazole sole application or with micronutrient significantly increased the lignin content by improving the lignin-related enzyme activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, tyrosine ammonialyase, and peroxidase, ameliorating basal internode characteristics, and breaking strength. The spike length, spike diameter, spikes/plant, weight/spike, yield/spike, and grain yield increased and then decreased with uniconazole application at a higher concentration, where their maximum values were recorded with UMS2 and US2 treatments. The lignin accumulation was positively correlated with lignin-related enzyme activities and breaking strength while, negatively correlated with lodging rate. Uniconazole significantly improved the lignin biosynthesis, lodging resistance, and grain yield of winter wheat and the treatments which showed the greatest effects were uniconazole seed soaking with micronutrient at a concentration of 30 mg L^-1 and 0.06 g L^-1 , and uniconazole sole seed soaking at a concentration of 30 mg L^-1 .展开更多
Plants are the ultimate source of nutrients in the human diet. To ensure adequate availability of high quality food for an increasing world population, traits including improved tolerance of stresses and nutrient leve...Plants are the ultimate source of nutrients in the human diet. To ensure adequate availability of high quality food for an increasing world population, traits including improved tolerance of stresses and nutrient levels need to be selected in crops, both individually and in combination. Here we report the identification of SIMX1 encoding a MIXTA-like MYB transcription factor in tomato that simultaneously modulates drought resistance and metabolic processes through regulating key structural and regulatory genes of the corre- sponding pathways. Over-expression of SIMX1 results in substantially increased drought tolerance and improved fruit quality, while knocking down SIMX1 resulted in the opposite phenotypes. Our study indicates an effective way with multiplebeneficial traits by genetic engineering of a single regulatory gene and can be a novel approach to breeding crops.展开更多
Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the sal...Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.展开更多
Large-scale crop monocultures facilitate the proliferation and increasing prevalence of diseases and pest insects.Many studies highlight the impacts of plant diversification upon pathogens,and the population dynamics ...Large-scale crop monocultures facilitate the proliferation and increasing prevalence of diseases and pest insects.Many studies highlight the impacts of plant diversification upon pathogens,and the population dynamics of insects and beneficial organism in agricultural ecosystems.These studies provide evidence that habitat manipulation techniques such as intercropping,relay,and rotation can significantly improve disease and pest management.This review introduces the concept of crop diversity,considers recent insights and mechanisms underlying crop diversity,and discusses its potential for improving sustainable agricultural practices.Recently,the phytobiomes resulting from increased crop diversity are increasingly recognized for their contribution to disease and pest control.Further,understanding the interactions between pathogens or pests with their host phytobiome may lead to novel options for the prevention of pests.Recent advances in the agricultural systems include:(i)a better understanding of the mechanisms of interactions between crop species and genotypes;(ii)ecological progress including a better understanding of the context-dependency of those interactions;and(iii)the role of microtopographic variation in agricultural systems for priming basal resistance to multiple pests and pathogens by intercropped crops.We also highlight recent progress in China and the potential options for habitat management and design that enhance the ecological role of biodiversity in agroecosystems.展开更多
Background:Endoscopic ultrasound (EUS) is a useful diagnostic and therapeutic tool in the pediatric population.Given the high accuracy and sensitivity of EUS,it is particularly effective in evaluating pancreaticobilia...Background:Endoscopic ultrasound (EUS) is a useful diagnostic and therapeutic tool in the pediatric population.Given the high accuracy and sensitivity of EUS,it is particularly effective in evaluating pancreaticobiliary disease.Published literature in the use of pediatric EUS is limited.Therefore we aimed to review the current literature for EUS indications,safety,and effectiveness for the pediatric population.Data sources:English language articles on the use of pediatric endoscopic ultrasound in evaluating pancreaticobiliary diseases were retrieved from PubMed/MEDLINE.Results:We analyzed various retrospective studies and case series publications.Data were extrapolated for pediatric patients with pancreaticobiliary diseases.Conclusions:EUS offers superior imaging,.It is comparible to magnetic resonance imaging and/or pancreatic-protocol computed tomography.In the current literature,there are a variety of pancreaticobiliary conditions where EUS was utilized to make a diagnosis.These include recurrent pancreatitis,congenital anomalies,microlithiasis,pancreatic pseudocysts,and pancreatic mass lesions.EUS was shown to be a safe and cost-effective modality with both diagnostic and therapeutic capabilities in the pediatric population.EUS is now increasingly being recognized as a standard of care when evaluating pancreaticobiliary conditions in children.展开更多
Background:Cotton is an important fiber crop worldwide.The yield potential of current genotypes of cotton can be exploited through hybridization.However,to develop superior hybrids with high yield and fiber quality tr...Background:Cotton is an important fiber crop worldwide.The yield potential of current genotypes of cotton can be exploited through hybridization.However,to develop superior hybrids with high yield and fiber quality traits,information of genetic control of traits is prerequisite.Therefore,genetic analysis plays pivotal role in plant breeding.Results:In present study,North Carolina II mating design was used to cross 5 female parents with 6 male parents to produce 30 intraspecific F1cotton hybrids.All plant materials were tested in three different ecological regions of China during the year of 2016-2017.Additive-dominance-environment(ADE)genetic model was used to estimate the genetic effects and genotypic and phenotypic correlation of yield and fiber quality traits.Results showed that yield traits except lint percentage were mainly controlled by genetic and environment interaction effects,whereas lint percentage and fiber quality traits were determined by main genetic effects.Moreover,dominant and additiveen vironine nt in teraction effects had more influence on yield traits,whereas additive and domi nance-e nviron ment interaction effects were found to be predominant for fiber traits.Broad-sense and its interaction heritability were significant for all yield and most of fiber quality traits.Narrow-sense and its interaction heritability were non-significant for boll number and seed cotton yield.Correlation analysis indicated that seed cotton yield had significant positive correlation with other yield attributes and non-significant with fiber quality traits.All fiber quality traits had signiflcant positive correlation with each other except micronaire.Conclusions:Results of current study provide important information about genetic control of yield and fiber quality traits.Further,this study identified that parental lines,e.g.,SJ48-1,ZB-1,851-2,and DT-8 can be utilized to improve yield and fiber quality traits in cotton.展开更多
Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization.Our previous study reported that manipulation of Sl MX1 gene enha...Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization.Our previous study reported that manipulation of Sl MX1 gene enhanced carotenoids accumulation and drought resistance in tomato.Here,RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of Sl MX1 involved in stress resistance and enhanced secondary metabolites.Physiological analysis showed that overexpression of Sl MX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic(fungus,bacteria,virus and insects) stresses in tomato.This research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome density.In addition,we reported two types of trichome which seems to be aberrant types in tomato.This study unravels the mechanism of regulation of Sl MX1,which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.展开更多
Depression is the most prevalent debilitating mental illness; it is characterized as a disorder of mood, cognitive function, and neurovegetative function. About one in ten individuals experience depression at some sta...Depression is the most prevalent debilitating mental illness; it is characterized as a disorder of mood, cognitive function, and neurovegetative function. About one in ten individuals experience depression at some stage of their lives. Antidepressant drugs are used to reduce the symptoms but relapse occurs in ~ 20% of patients. However, alternate therapies like brain stimulation techniques have shown promising results in this regard. This review covers the brain stimulation techniques electroconvulsive therapy, transcranial direct current stimulation, repetitive transcranial magnetic stimulation, vagus nerve stimulation, and deep brain stimulation, which are used as alternatives to antide- pressant drugs, and elucidates their research and clinical outcomes.展开更多
文摘Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypo-cotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.
基金supported by funding from the National High-Tech R&D Program of China (2013AA102902)the Program of Introducing Talents of Discipline to Universities, China (B12007)the National Natural Science Foundation of China (31601257)
文摘Lodging stress results in grain yield and quality reduction in wheat. Uniconazole, a potential plant growth regulator significantly enhances lignin biosynthesis and thus provides mechanical strength to plants in order to cope with lodging stress. A field study was conducted during the 2015–2016 and 2016–2017 growing seasons, to investigate the effects of uniconazole sole application or with micronutrient on the lignin biosynthesis, lodging resistance, and production of winter wheat. In the first experiment, uniconazole at concentrations of 0(CK), 15(US1), 30(US2), and 45(US3) mg L^-1 was applied as sole seed soaking, while in the second experiment with manganese(Mn) at concentration of 0.06 g L^-1 Mn, 0.06 g L^-1 Mn+ 15 mg L^-1 uniconazole(UMS1), 0.06 g L^-1 Mn+30 mg L^-1 uniconazole(UMS2), and 0.06 g L^-1 Mn+45 mg L^-1 uniconazole(UMS3), respectively. Uniconazole sole application or with micronutrient significantly increased the lignin content by improving the lignin-related enzyme activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, tyrosine ammonialyase, and peroxidase, ameliorating basal internode characteristics, and breaking strength. The spike length, spike diameter, spikes/plant, weight/spike, yield/spike, and grain yield increased and then decreased with uniconazole application at a higher concentration, where their maximum values were recorded with UMS2 and US2 treatments. The lignin accumulation was positively correlated with lignin-related enzyme activities and breaking strength while, negatively correlated with lodging rate. Uniconazole significantly improved the lignin biosynthesis, lodging resistance, and grain yield of winter wheat and the treatments which showed the greatest effects were uniconazole seed soaking with micronutrient at a concentration of 30 mg L^-1 and 0.06 g L^-1 , and uniconazole sole seed soaking at a concentration of 30 mg L^-1 .
基金supported by the Major State Basic Research Development Program of China (2011CB100601)
文摘Plants are the ultimate source of nutrients in the human diet. To ensure adequate availability of high quality food for an increasing world population, traits including improved tolerance of stresses and nutrient levels need to be selected in crops, both individually and in combination. Here we report the identification of SIMX1 encoding a MIXTA-like MYB transcription factor in tomato that simultaneously modulates drought resistance and metabolic processes through regulating key structural and regulatory genes of the corre- sponding pathways. Over-expression of SIMX1 results in substantially increased drought tolerance and improved fruit quality, while knocking down SIMX1 resulted in the opposite phenotypes. Our study indicates an effective way with multiplebeneficial traits by genetic engineering of a single regulatory gene and can be a novel approach to breeding crops.
基金funded by the Key-Area Research and Development Program of Guangdong Province,China(Grant No.2020B020219004)the Hainan Yazhou Bay Seed Lab(Grant No.B21HJ0216)the Agricultural Science and Technology Innovation Program and the Cooperation and Innovation Mission,China(Grant No.CAAS-ZDXT202001)。
文摘Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.
基金financially supported by the National Key Research & Development Program of China (2017YFD0200400)
文摘Large-scale crop monocultures facilitate the proliferation and increasing prevalence of diseases and pest insects.Many studies highlight the impacts of plant diversification upon pathogens,and the population dynamics of insects and beneficial organism in agricultural ecosystems.These studies provide evidence that habitat manipulation techniques such as intercropping,relay,and rotation can significantly improve disease and pest management.This review introduces the concept of crop diversity,considers recent insights and mechanisms underlying crop diversity,and discusses its potential for improving sustainable agricultural practices.Recently,the phytobiomes resulting from increased crop diversity are increasingly recognized for their contribution to disease and pest control.Further,understanding the interactions between pathogens or pests with their host phytobiome may lead to novel options for the prevention of pests.Recent advances in the agricultural systems include:(i)a better understanding of the mechanisms of interactions between crop species and genotypes;(ii)ecological progress including a better understanding of the context-dependency of those interactions;and(iii)the role of microtopographic variation in agricultural systems for priming basal resistance to multiple pests and pathogens by intercropped crops.We also highlight recent progress in China and the potential options for habitat management and design that enhance the ecological role of biodiversity in agroecosystems.
文摘Background:Endoscopic ultrasound (EUS) is a useful diagnostic and therapeutic tool in the pediatric population.Given the high accuracy and sensitivity of EUS,it is particularly effective in evaluating pancreaticobiliary disease.Published literature in the use of pediatric EUS is limited.Therefore we aimed to review the current literature for EUS indications,safety,and effectiveness for the pediatric population.Data sources:English language articles on the use of pediatric endoscopic ultrasound in evaluating pancreaticobiliary diseases were retrieved from PubMed/MEDLINE.Results:We analyzed various retrospective studies and case series publications.Data were extrapolated for pediatric patients with pancreaticobiliary diseases.Conclusions:EUS offers superior imaging,.It is comparible to magnetic resonance imaging and/or pancreatic-protocol computed tomography.In the current literature,there are a variety of pancreaticobiliary conditions where EUS was utilized to make a diagnosis.These include recurrent pancreatitis,congenital anomalies,microlithiasis,pancreatic pseudocysts,and pancreatic mass lesions.EUS was shown to be a safe and cost-effective modality with both diagnostic and therapeutic capabilities in the pediatric population.EUS is now increasingly being recognized as a standard of care when evaluating pancreaticobiliary conditions in children.
基金supported by National Key Research and Development Program of China(2016YFD0101400)
文摘Background:Cotton is an important fiber crop worldwide.The yield potential of current genotypes of cotton can be exploited through hybridization.However,to develop superior hybrids with high yield and fiber quality traits,information of genetic control of traits is prerequisite.Therefore,genetic analysis plays pivotal role in plant breeding.Results:In present study,North Carolina II mating design was used to cross 5 female parents with 6 male parents to produce 30 intraspecific F1cotton hybrids.All plant materials were tested in three different ecological regions of China during the year of 2016-2017.Additive-dominance-environment(ADE)genetic model was used to estimate the genetic effects and genotypic and phenotypic correlation of yield and fiber quality traits.Results showed that yield traits except lint percentage were mainly controlled by genetic and environment interaction effects,whereas lint percentage and fiber quality traits were determined by main genetic effects.Moreover,dominant and additiveen vironine nt in teraction effects had more influence on yield traits,whereas additive and domi nance-e nviron ment interaction effects were found to be predominant for fiber traits.Broad-sense and its interaction heritability were significant for all yield and most of fiber quality traits.Narrow-sense and its interaction heritability were non-significant for boll number and seed cotton yield.Correlation analysis indicated that seed cotton yield had significant positive correlation with other yield attributes and non-significant with fiber quality traits.All fiber quality traits had signiflcant positive correlation with each other except micronaire.Conclusions:Results of current study provide important information about genetic control of yield and fiber quality traits.Further,this study identified that parental lines,e.g.,SJ48-1,ZB-1,851-2,and DT-8 can be utilized to improve yield and fiber quality traits in cotton.
基金supported by the Major State Basic Research Development Program of China(2011CB100601)the National Natural Science Foundation of China(31070267)the Program for New Century Excellent Talents in University of Ministry of Education in China(NCET-09-0401)
文摘Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization.Our previous study reported that manipulation of Sl MX1 gene enhanced carotenoids accumulation and drought resistance in tomato.Here,RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of Sl MX1 involved in stress resistance and enhanced secondary metabolites.Physiological analysis showed that overexpression of Sl MX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic(fungus,bacteria,virus and insects) stresses in tomato.This research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome density.In addition,we reported two types of trichome which seems to be aberrant types in tomato.This study unravels the mechanism of regulation of Sl MX1,which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.
文摘Depression is the most prevalent debilitating mental illness; it is characterized as a disorder of mood, cognitive function, and neurovegetative function. About one in ten individuals experience depression at some stage of their lives. Antidepressant drugs are used to reduce the symptoms but relapse occurs in ~ 20% of patients. However, alternate therapies like brain stimulation techniques have shown promising results in this regard. This review covers the brain stimulation techniques electroconvulsive therapy, transcranial direct current stimulation, repetitive transcranial magnetic stimulation, vagus nerve stimulation, and deep brain stimulation, which are used as alternatives to antide- pressant drugs, and elucidates their research and clinical outcomes.
