目的:在成功制备小鼠抗人IL-13Rα2高亲和力单克隆抗体(mAb)的基础上,通过分子克隆方法获得该抗体可变区基因序列。方法:从1株小鼠抗人IL-13Rα2 mAb杂交瘤细胞LX147-7中提取总RNA,以此为模板反转录获得cDNA,用针对小鼠mAb重链和轻链可...目的:在成功制备小鼠抗人IL-13Rα2高亲和力单克隆抗体(mAb)的基础上,通过分子克隆方法获得该抗体可变区基因序列。方法:从1株小鼠抗人IL-13Rα2 mAb杂交瘤细胞LX147-7中提取总RNA,以此为模板反转录获得cDNA,用针对小鼠mAb重链和轻链可变区基因序列的特异性引物分别进行PCR反应。将PCR产物连入克隆载体,经筛选阳性克隆,PCR和酶切鉴定正确后送测序,测序结果进行生物信息学分析。结果:成功克隆了抗人IL-13Rα2 mAb LX147-7重链和轻链的可变区基因。结论:获得了抗人IL-13Rα2 mAb LX147-7重链和轻链的可变区基因序列,为构建相关基因工程抗体打下了良好基础。展开更多
To investigate the static compressive properties and mechanical damage evolution of rubber cement-based materials(RCBMs) with dry-and wet-curing conditions, uniaxial compression and cyclic loading-unloading tests were...To investigate the static compressive properties and mechanical damage evolution of rubber cement-based materials(RCBMs) with dry-and wet-curing conditions, uniaxial compression and cyclic loading-unloading tests were carried out on rubber cement mortar(RCM). The mechanical properties of the uniaxial compression specimens cured at 95%(wet-curing) and 50%(dry-curing) relative humidities and cyclic loading-unloading specimens cured at wet-curing were analyzed. Under uniaxial compression, the peak stress loss ratio is higher for dry-curing than for wet-curing. The peak strain decreases with the increase of rubber content, and the peak strain increases with the decrease of curing humidity. Under cyclic loading-unloading, the variation trends of residual strain differences of the normal cement mortar and RCM at each cyclic level with the number of cycles are basically the same, but the failure modes are different. The analysis of the internal mesostructure by a scanning electron microscope(SEM) shows that initial damage is further enhanced by reducing curing humidity and adding rubber aggregate. The damage constitutive model based on strain equivalence principle and statistical theories was used to describe the uniaxial compression characteristics of RCM, and the law of mechanical damage evolution was predicted.展开更多
文摘目的:在成功制备小鼠抗人IL-13Rα2高亲和力单克隆抗体(mAb)的基础上,通过分子克隆方法获得该抗体可变区基因序列。方法:从1株小鼠抗人IL-13Rα2 mAb杂交瘤细胞LX147-7中提取总RNA,以此为模板反转录获得cDNA,用针对小鼠mAb重链和轻链可变区基因序列的特异性引物分别进行PCR反应。将PCR产物连入克隆载体,经筛选阳性克隆,PCR和酶切鉴定正确后送测序,测序结果进行生物信息学分析。结果:成功克隆了抗人IL-13Rα2 mAb LX147-7重链和轻链的可变区基因。结论:获得了抗人IL-13Rα2 mAb LX147-7重链和轻链的可变区基因序列,为构建相关基因工程抗体打下了良好基础。
基金Projects(52008003,52074009)supported by the National Natural Science Foundation of ChinaProject(201904a07020081)supported by the Key Research and Development Program Project of Anhui Province,ChinaProject(1908085QE213)supported by the Nature Science Foundation of Anhui Province,China。
文摘To investigate the static compressive properties and mechanical damage evolution of rubber cement-based materials(RCBMs) with dry-and wet-curing conditions, uniaxial compression and cyclic loading-unloading tests were carried out on rubber cement mortar(RCM). The mechanical properties of the uniaxial compression specimens cured at 95%(wet-curing) and 50%(dry-curing) relative humidities and cyclic loading-unloading specimens cured at wet-curing were analyzed. Under uniaxial compression, the peak stress loss ratio is higher for dry-curing than for wet-curing. The peak strain decreases with the increase of rubber content, and the peak strain increases with the decrease of curing humidity. Under cyclic loading-unloading, the variation trends of residual strain differences of the normal cement mortar and RCM at each cyclic level with the number of cycles are basically the same, but the failure modes are different. The analysis of the internal mesostructure by a scanning electron microscope(SEM) shows that initial damage is further enhanced by reducing curing humidity and adding rubber aggregate. The damage constitutive model based on strain equivalence principle and statistical theories was used to describe the uniaxial compression characteristics of RCM, and the law of mechanical damage evolution was predicted.
