Gossypol-induced alterations of aminophospholipid composition in human sperm wereobserved and the effects of some factors on these alterations were investigated.The altera-tions of aminophospholipid composition of hum...Gossypol-induced alterations of aminophospholipid composition in human sperm wereobserved and the effects of some factors on these alterations were investigated.The altera-tions of aminophospholipid composition of human sperm induced by gossypoI included aprogressive decrease in the levels of phosphatidylethanolamine（PE）and phosphatidylserine（PS）when gossypol concentrations ranged from 5-500μmol/L,and a progressive increase inthe level of（LPE）at lower gossypol concentrations（5-50μmol/L）.The conversion of PEinto lysophosphatidylethanolamine（LPE）was strongly enhanced by Ca<sup>2+</sup>and inhibitedby 0.5mmol/L EDTA,while PMSF,NEM,iodoacetamide and Zn<sup>2+</sup>had no effect.Thisconversion was weaker in sperm with stripped surface proteins than in normal sperm.In addition,three surface proteins（MW 80,60 and 40 kD）were fixed on the plasmamembrane by gossypol treatment.The significance of gossypol action and the possibilitythat sperm PLA<sub>2</sub>, PE and.some surface proteins might play an important role in the physio-logical acrosome reaetion of human sperm,as well as in oocyte penetration,are discussed.展开更多
The fibroneetin from human early placenta (5-8 weeks) (tFN) has been isolated.According to solubilio it could be divided into PBS-soluble and PBS-insotubte. Quan-titative assay by EL1SA indicated that the PBS soluble ...The fibroneetin from human early placenta (5-8 weeks) (tFN) has been isolated.According to solubilio it could be divided into PBS-soluble and PBS-insotubte. Quan-titative assay by EL1SA indicated that the PBS soluble tFN was about 25.4% of thetotal FN-content, an average of 0.35rag in per gram tissues (wet weight),SDS-PAG E, Western blots and the analysis of the affinity to MoAbs against three functional demains of amniotie fluid fibronectin (amFN) and the binding capacity to.five lectins showed that the tFN was different from human ptasma fibronectin( pFN ) aswelt as human term palcenta.,fibronectin( tpFN). The preliminary result suaggested tous that there might be a special fibronectin isotype in human earlv ptaeenta which maybe as a target for immunocontraception.展开更多
文摘Gossypol-induced alterations of aminophospholipid composition in human sperm wereobserved and the effects of some factors on these alterations were investigated.The altera-tions of aminophospholipid composition of human sperm induced by gossypoI included aprogressive decrease in the levels of phosphatidylethanolamine（PE）and phosphatidylserine（PS）when gossypol concentrations ranged from 5-500μmol/L,and a progressive increase inthe level of（LPE）at lower gossypol concentrations（5-50μmol/L）.The conversion of PEinto lysophosphatidylethanolamine（LPE）was strongly enhanced by Ca<sup>2+</sup>and inhibitedby 0.5mmol/L EDTA,while PMSF,NEM,iodoacetamide and Zn<sup>2+</sup>had no effect.Thisconversion was weaker in sperm with stripped surface proteins than in normal sperm.In addition,three surface proteins（MW 80,60 and 40 kD）were fixed on the plasmamembrane by gossypol treatment.The significance of gossypol action and the possibilitythat sperm PLA<sub>2</sub>, PE and.some surface proteins might play an important role in the physio-logical acrosome reaetion of human sperm,as well as in oocyte penetration,are discussed.
文摘The fibroneetin from human early placenta (5-8 weeks) (tFN) has been isolated.According to solubilio it could be divided into PBS-soluble and PBS-insotubte. Quan-titative assay by EL1SA indicated that the PBS soluble tFN was about 25.4% of thetotal FN-content, an average of 0.35rag in per gram tissues (wet weight),SDS-PAG E, Western blots and the analysis of the affinity to MoAbs against three functional demains of amniotie fluid fibronectin (amFN) and the binding capacity to.five lectins showed that the tFN was different from human ptasma fibronectin( pFN ) aswelt as human term palcenta.,fibronectin( tpFN). The preliminary result suaggested tous that there might be a special fibronectin isotype in human earlv ptaeenta which maybe as a target for immunocontraception.
