[Objective] The aim was to understand genetic diversity and genetic differ- entiation situation of wild V. philippinarum population in the coast of China. [Methed] Based on PCR technology, CO I gene fragments of the m...[Objective] The aim was to understand genetic diversity and genetic differ- entiation situation of wild V. philippinarum population in the coast of China. [Methed] Based on PCR technology, CO I gene fragments of the mitochondria were amplified from two populations of Yantai and Weihai for sequence analysis. [Result] Fifty sam- ples all obtained CO I gene fragment sequence of 684 bp. A total of 42 haplotypes were defined, and 62 variation loci were detected. The haplotypes diversity of two populations was both high, while the nucleotide diversity was low. The phylogenetic tree of haplotypes established by maximum likelihood （ML） method showed that the samples of two geographic populations presented diffused distribution and had no obvious population differentiation. [Conclusion] The result indicated that the gene ex- change of marine animal is easy, and the separation among different seas is weak.展开更多
基金Supported by National Natural Science Foundation of China (30800605)Natural Science Foundation of Shandong Province (ZR2009DZ003)~~
文摘[Objective] The aim was to understand genetic diversity and genetic differ- entiation situation of wild V. philippinarum population in the coast of China. [Methed] Based on PCR technology, CO I gene fragments of the mitochondria were amplified from two populations of Yantai and Weihai for sequence analysis. [Result] Fifty sam- ples all obtained CO I gene fragment sequence of 684 bp. A total of 42 haplotypes were defined, and 62 variation loci were detected. The haplotypes diversity of two populations was both high, while the nucleotide diversity was low. The phylogenetic tree of haplotypes established by maximum likelihood （ML） method showed that the samples of two geographic populations presented diffused distribution and had no obvious population differentiation. [Conclusion] The result indicated that the gene ex- change of marine animal is easy, and the separation among different seas is weak.
