金环蛇(Bungarus fasciatus)蛇毒经磷酸纤维素P11柱层析分离所得的第8峰(即类心脏毒素),再经磺乙基—葡聚糖凝胶G—25柱层析分离得8A、8B。再将8A、8B分别经葡聚糖凝胶G—50柱层析,得纯化的类心脏毒素A、B。经聚丙烯酰胺凝胶电泳鉴定为...金环蛇(Bungarus fasciatus)蛇毒经磷酸纤维素P11柱层析分离所得的第8峰(即类心脏毒素),再经磺乙基—葡聚糖凝胶G—25柱层析分离得8A、8B。再将8A、8B分别经葡聚糖凝胶G—50柱层析,得纯化的类心脏毒素A、B。经聚丙烯酰胺凝胶电泳鉴定为单一的条带。无酶活性。其氨基酸组成及含量基本与Shiau Lin 1975等所测定的结果一致。展开更多
应用快原子轰击酶谱分析检验了东亚马氏钳蝎哺乳动物类神经毒素Ⅲ的氨基酸序列,对前人用 Edman 降解法测定的序列作了两处修正:(1)第60位氨基酸残基是脯氨酸而不是色氨酸;(2)(?)-端氨基酸是组氨酸而不是甘氨酸.分别用羧肽酶 B 降解和部...应用快原子轰击酶谱分析检验了东亚马氏钳蝎哺乳动物类神经毒素Ⅲ的氨基酸序列,对前人用 Edman 降解法测定的序列作了两处修正:(1)第60位氨基酸残基是脯氨酸而不是色氨酸;(2)(?)-端氨基酸是组氨酸而不是甘氨酸.分别用羧肽酶 B 降解和部分胰蛋白酶酶解后的质谱数据进一步证实了以上的修正.在分析中采用了化学修饰方法以减少表面抑制效应,从而得到完整的快原子轰击酶谱.展开更多
The neurotoxins from scorpions are one kind of low molecular toxic proteins which can bind with sodium channels of excitable membranes. They have aroused intense scientific interest because of the potential use of sco...The neurotoxins from scorpions are one kind of low molecular toxic proteins which can bind with sodium channels of excitable membranes. They have aroused intense scientific interest because of the potential use of scorpion toxins as tools for neurobiological studies. We reported previously the isolation and purification of neurotoxins of scorpion venom which was collected from Yidu region, Shandong Province, (Bi-展开更多
文摘金环蛇(Bungarus fasciatus)蛇毒经磷酸纤维素P11柱层析分离所得的第8峰(即类心脏毒素),再经磺乙基—葡聚糖凝胶G—25柱层析分离得8A、8B。再将8A、8B分别经葡聚糖凝胶G—50柱层析,得纯化的类心脏毒素A、B。经聚丙烯酰胺凝胶电泳鉴定为单一的条带。无酶活性。其氨基酸组成及含量基本与Shiau Lin 1975等所测定的结果一致。
文摘应用快原子轰击酶谱分析检验了东亚马氏钳蝎哺乳动物类神经毒素Ⅲ的氨基酸序列,对前人用 Edman 降解法测定的序列作了两处修正:(1)第60位氨基酸残基是脯氨酸而不是色氨酸;(2)(?)-端氨基酸是组氨酸而不是甘氨酸.分别用羧肽酶 B 降解和部分胰蛋白酶酶解后的质谱数据进一步证实了以上的修正.在分析中采用了化学修饰方法以减少表面抑制效应,从而得到完整的快原子轰击酶谱.
基金Project supported by the National Natural Science Foundation of China (3860166)
文摘The neurotoxins from scorpions are one kind of low molecular toxic proteins which can bind with sodium channels of excitable membranes. They have aroused intense scientific interest because of the potential use of scorpion toxins as tools for neurobiological studies. We reported previously the isolation and purification of neurotoxins of scorpion venom which was collected from Yidu region, Shandong Province, (Bi-
