目的对大鼠中度脊髓损伤后两种后肢行为学评估标准进行评价。方法24只雄性Wistar大鼠随机分为假手术组和损伤组。损伤组采用Allen's打击法制作脊髓损伤模型,假手术组仅行椎板切除术。术后1、3、7、14、21天分别采用Tarlov评分和BBB(...目的对大鼠中度脊髓损伤后两种后肢行为学评估标准进行评价。方法24只雄性Wistar大鼠随机分为假手术组和损伤组。损伤组采用Allen's打击法制作脊髓损伤模型,假手术组仅行椎板切除术。术后1、3、7、14、21天分别采用Tarlov评分和BBB(basso,beattie and bresnahan)评分对两组大鼠后肢运动功能恢复情况进行评估。结果两组大鼠BBB评分值在伤后各时间点差异均非常显著(P<0.01),Tarlov评分值在伤后各时间点有显著性差异(P<0.05),但只在1、3、7天时差异才非常显著(P<0.01);评分值按百分制换算后,假手术组大鼠两组评分值之间的一致性较好,仅在伤后7天时有显著性差异(P<0.05),损伤组大鼠两组评分值之间的差异较大,伤后1、3、7天时差异非常显著(P<0.01)。结论BBB评分的敏感性和区分度优于Tarlov评分,能更准确、客观地反映大鼠中度脊髓损伤后运动功能恢复情况。展开更多
To find a new source of seed cells for constructing tissue-engineered intervertebral disc, nucleus pulposus （NP） cells and mesenchymal stem cells （MSCs） were isolated from New Zealand white rabbits. The nucleus pu...To find a new source of seed cells for constructing tissue-engineered intervertebral disc, nucleus pulposus （NP） cells and mesenchymal stem cells （MSCs） were isolated from New Zealand white rabbits. The nucleus pulposus cells population was fluorescence-ladled and co-cultured with MSCs with or without direct contact. Morphological changes were observed every 12 h. Semi-quantitaive reverse transcriptase-polymerase chain reaction was performed to assess the expression levels of Sox-9, aggreacan and type Ⅱ collagen every 24 h after the co-culture. MSCs treated with direct contact rounded up and presented a ring-like appearance. The expression of marker genes was significantly increased when cells were co-cultured with direct contact for 24 h. No significant change was found after coculture without direct contact. Co-culture of NP cells and MSCs with direct contact is a reliable method for generating large amount of NP cells used for cell-based tissue engineering therapy.展开更多
重症多发伤是由外界高能量暴力造成的2个以上解剖部位的严重机体创伤,患者伤情复杂,容易导致多器官功能障碍,获得及时、有效的治疗是改善其预后的重要保证[1]。"损伤控制"是以分阶段的手术方法来管理重症多发伤患者的一种分...重症多发伤是由外界高能量暴力造成的2个以上解剖部位的严重机体创伤,患者伤情复杂,容易导致多器官功能障碍,获得及时、有效的治疗是改善其预后的重要保证[1]。"损伤控制"是以分阶段的手术方法来管理重症多发伤患者的一种分步救治策略,其主要内容包括初期稳定生命体征的救治措施,重症监护室(intensive care unit,ICU)的复苏治疗和后期对明确伤害的修复和重建[2]。在我国,由于医疗资源分布不均匀,偏远地区的重症多发伤患者常因得不到及时、有效的治疗而延误病情。展开更多
文摘目的对大鼠中度脊髓损伤后两种后肢行为学评估标准进行评价。方法24只雄性Wistar大鼠随机分为假手术组和损伤组。损伤组采用Allen's打击法制作脊髓损伤模型,假手术组仅行椎板切除术。术后1、3、7、14、21天分别采用Tarlov评分和BBB(basso,beattie and bresnahan)评分对两组大鼠后肢运动功能恢复情况进行评估。结果两组大鼠BBB评分值在伤后各时间点差异均非常显著(P<0.01),Tarlov评分值在伤后各时间点有显著性差异(P<0.05),但只在1、3、7天时差异才非常显著(P<0.01);评分值按百分制换算后,假手术组大鼠两组评分值之间的一致性较好,仅在伤后7天时有显著性差异(P<0.05),损伤组大鼠两组评分值之间的差异较大,伤后1、3、7天时差异非常显著(P<0.01)。结论BBB评分的敏感性和区分度优于Tarlov评分,能更准确、客观地反映大鼠中度脊髓损伤后运动功能恢复情况。
基金a grant from the National Natural Sciences Foundation of China (No. 30772206)
文摘To find a new source of seed cells for constructing tissue-engineered intervertebral disc, nucleus pulposus （NP） cells and mesenchymal stem cells （MSCs） were isolated from New Zealand white rabbits. The nucleus pulposus cells population was fluorescence-ladled and co-cultured with MSCs with or without direct contact. Morphological changes were observed every 12 h. Semi-quantitaive reverse transcriptase-polymerase chain reaction was performed to assess the expression levels of Sox-9, aggreacan and type Ⅱ collagen every 24 h after the co-culture. MSCs treated with direct contact rounded up and presented a ring-like appearance. The expression of marker genes was significantly increased when cells were co-cultured with direct contact for 24 h. No significant change was found after coculture without direct contact. Co-culture of NP cells and MSCs with direct contact is a reliable method for generating large amount of NP cells used for cell-based tissue engineering therapy.
文摘重症多发伤是由外界高能量暴力造成的2个以上解剖部位的严重机体创伤,患者伤情复杂,容易导致多器官功能障碍,获得及时、有效的治疗是改善其预后的重要保证[1]。"损伤控制"是以分阶段的手术方法来管理重症多发伤患者的一种分步救治策略,其主要内容包括初期稳定生命体征的救治措施,重症监护室(intensive care unit,ICU)的复苏治疗和后期对明确伤害的修复和重建[2]。在我国,由于医疗资源分布不均匀,偏远地区的重症多发伤患者常因得不到及时、有效的治疗而延误病情。
