To investigate telomerase activity in rabbit bone marrow stromal cells （BMSCs） during their committed differentiation in vitro along neural pathway and the effect of glial cell line-derived neurotrophic factor （GDN...To investigate telomerase activity in rabbit bone marrow stromal cells （BMSCs） during their committed differentiation in vitro along neural pathway and the effect of glial cell line-derived neurotrophic factor （GDNF） on the expression of telomerase. Methods ： BMSCs were acquired from rabbit marrow and divided into control group, GDNF （10 ng/ml） group. Cytokine . NSCs medium （prepared by our lab, Patent No. ZL02134314. 4） supplemented with 10% fetal bovine serum （FBS） was used to induce BMSCs differentiation along neural pathway. Fluorescent was employed to identify the expressions of Nestin, neuronspecific endase （NSE）, and gial fibrillary acidic protein （GFAP）. The growth curves of the cells and the status of cell cycles were analyzed, respectively. During the differentiation, telomerase activitys were detected using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay （TRAP-ELISA）. Results： BMSCs were successfully induced to differentiate along neural pathway and expressed specific markers of fetal neural epithelium, mature neuron and glial cells. Telomeruse activities were undetectahle in BMSCs during differentiation along neural pathway. Similar changes of cell growth curves, cell cycle status and telomeruse expression were observed in the two groups. Conclusions ： Rabbit BMSCs do not display telomeruse activity during differentiation along neural pathway. GDNF shows little impact on proliferation and telomeruse activity of BMSCs.展开更多
基金This project was supported by National Natural Science Foundation of China ( No. 30270491 ,30400464) .
文摘To investigate telomerase activity in rabbit bone marrow stromal cells （BMSCs） during their committed differentiation in vitro along neural pathway and the effect of glial cell line-derived neurotrophic factor （GDNF） on the expression of telomerase. Methods ： BMSCs were acquired from rabbit marrow and divided into control group, GDNF （10 ng/ml） group. Cytokine . NSCs medium （prepared by our lab, Patent No. ZL02134314. 4） supplemented with 10% fetal bovine serum （FBS） was used to induce BMSCs differentiation along neural pathway. Fluorescent was employed to identify the expressions of Nestin, neuronspecific endase （NSE）, and gial fibrillary acidic protein （GFAP）. The growth curves of the cells and the status of cell cycles were analyzed, respectively. During the differentiation, telomerase activitys were detected using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay （TRAP-ELISA）. Results： BMSCs were successfully induced to differentiate along neural pathway and expressed specific markers of fetal neural epithelium, mature neuron and glial cells. Telomeruse activities were undetectahle in BMSCs during differentiation along neural pathway. Similar changes of cell growth curves, cell cycle status and telomeruse expression were observed in the two groups. Conclusions ： Rabbit BMSCs do not display telomeruse activity during differentiation along neural pathway. GDNF shows little impact on proliferation and telomeruse activity of BMSCs.
